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The map label for this gene is dapE

Identifier: 197103845

GI number: 197103845

Start: 383734

End: 384876

Strand: Reverse

Name: dapE

Synonym: PHZ_c0379

Alternate gene names: 197103845

Gene position: 384876-383734 (Counterclockwise)

Preceding gene: 197103849

Following gene: 197103842

Centisome position: 9.63

GC content: 71.65

Gene sequence:

>1143_bases
ATGAGCGCCGCCATAGACGCCGTCGAGCTTTCCCGCGAGCTGATCCGCAAGCCCTCCGTCACGCCCGCCGACGAGGGAGC
CATGGACGTGGTCGAACGCACCCTCGCAGGGCTGGGCTTCGCCTGCCGCCGGATGCGGTTCGGCGAGATCGAGAATCTCT
ACGCCCGCTACGGGACCGCCCGGCCGAACCTCTGCTTCGCCGGTCACACCGACGTGGTGCCGGTGGGCGACGCGGCCGCC
TGGTCCAAGGACGCCTTCGCCGCCGACGTGGTGGACGGGGTCCTGATCGGCCGCGGCGCCGTCGACATGAAGAGCGCCAT
CGCCGCCTTCGCCGCGGCCGCCGCCGAGGCGATCGCCGCCGGCCGGGTGACGGGCTCGGTCTCGTTCCTGATCACCGGCG
ACGAGGAGGGCGTGGCCACCCACGGCACCAAGAAGGTCGTCGAGGCGCTGCTGGCCGAGGGCGAGGCCATCGACCACTGC
GTGGTGGGCGAGCCGACCTCGGCCGAGTCGTTCGGCGACATGGTCAAGGTGGGCCGGCGCGGCTCGATCAACGCCGAGAT
CCTTGTCGAGGGAATCCAGGGGCACGTGGCCTATCCGCACCGGGCGGCCAACCCGGTCCCGGTGCTGGTTCGCCTGCTGG
CGGCCCTGCAGGATCGGGCGCTGGACGAGGGTTACCCCGAGTTCCAGCCCTCGAACCTGGAAGTGACCATGATCGACGTG
CCGAACACGGCGACGAACGTGATCCCCGGCACGGCGAAGGCGCGGCTCAACATCCGCTTCAATCCGAACCACACCGGCCA
GGCGCTGGCCGACTGGATCGCGGCCGAGGCGCGGAAGGCGGCCGACGGCTTCAAGGGCAAGGTCACCGTCACGCCCCAGA
TCAGCGGCGAGGCGTTCCTGACCGAGCGCGGCCCGTTCACCGAGCTGGTGGCGGCGGCCGTCAAGGACGTCACCGGCGCC
GAGCCCGAGCTCTCCACCTCGGGCGGGACGTCCGACGCCCGCTTCATCCGGGCGCTGTGCCCCGTCGTCGAGGTGGGCCT
CGTCGGCCGCACCATGCACCAGGTGGACGAGCGCGCGCCGGTGGACGAGATCCGCCGCCTGCAGGCCGTCTACGCCGCCA
TCATCGCCCGCTACTTCGCCTGA

Upstream 100 bases:

>100_bases
TGCGGATAGCTGCAGCCCGAGCGCGTCTCCAGCGTCTCGATGTCGATCCTCGGCATGGTCCCCCCTCGCCTCGCGTCCCA
ATGGCAGGTAATGCGGCGGC

Downstream 100 bases:

>100_bases
GGGGCCGCGGCGGGCGCCAGGCGCTCCAGCCGCATCACGAACGGCGGGTCGGCGTCCGGGTCGGCGCTGCGCGAGACGAC
GCCGGTGGCGCTCACCTTCT

Product: succinyl-diaminopimelate desuccinylase

Products: NA

Alternate protein names: SDAP desuccinylase; N-succinyl-LL-2,6-diaminoheptanedioate amidohydrolase

Number of amino acids: Translated: 380; Mature: 379

Protein sequence:

>380_residues
MSAAIDAVELSRELIRKPSVTPADEGAMDVVERTLAGLGFACRRMRFGEIENLYARYGTARPNLCFAGHTDVVPVGDAAA
WSKDAFAADVVDGVLIGRGAVDMKSAIAAFAAAAAEAIAAGRVTGSVSFLITGDEEGVATHGTKKVVEALLAEGEAIDHC
VVGEPTSAESFGDMVKVGRRGSINAEILVEGIQGHVAYPHRAANPVPVLVRLLAALQDRALDEGYPEFQPSNLEVTMIDV
PNTATNVIPGTAKARLNIRFNPNHTGQALADWIAAEARKAADGFKGKVTVTPQISGEAFLTERGPFTELVAAAVKDVTGA
EPELSTSGGTSDARFIRALCPVVEVGLVGRTMHQVDERAPVDEIRRLQAVYAAIIARYFA

Sequences:

>Translated_380_residues
MSAAIDAVELSRELIRKPSVTPADEGAMDVVERTLAGLGFACRRMRFGEIENLYARYGTARPNLCFAGHTDVVPVGDAAA
WSKDAFAADVVDGVLIGRGAVDMKSAIAAFAAAAAEAIAAGRVTGSVSFLITGDEEGVATHGTKKVVEALLAEGEAIDHC
VVGEPTSAESFGDMVKVGRRGSINAEILVEGIQGHVAYPHRAANPVPVLVRLLAALQDRALDEGYPEFQPSNLEVTMIDV
PNTATNVIPGTAKARLNIRFNPNHTGQALADWIAAEARKAADGFKGKVTVTPQISGEAFLTERGPFTELVAAAVKDVTGA
EPELSTSGGTSDARFIRALCPVVEVGLVGRTMHQVDERAPVDEIRRLQAVYAAIIARYFA
>Mature_379_residues
SAAIDAVELSRELIRKPSVTPADEGAMDVVERTLAGLGFACRRMRFGEIENLYARYGTARPNLCFAGHTDVVPVGDAAAW
SKDAFAADVVDGVLIGRGAVDMKSAIAAFAAAAAEAIAAGRVTGSVSFLITGDEEGVATHGTKKVVEALLAEGEAIDHCV
VGEPTSAESFGDMVKVGRRGSINAEILVEGIQGHVAYPHRAANPVPVLVRLLAALQDRALDEGYPEFQPSNLEVTMIDVP
NTATNVIPGTAKARLNIRFNPNHTGQALADWIAAEARKAADGFKGKVTVTPQISGEAFLTERGPFTELVAAAVKDVTGAE
PELSTSGGTSDARFIRALCPVVEVGLVGRTMHQVDERAPVDEIRRLQAVYAAIIARYFA

Specific function: Catalyzes the hydrolysis of N-succinyl-L,L- diaminopimelic acid (SDAP), forming succinate and LL-2,6- diaminoheptanedioate (DAP), an intermediate involved in the bacterial biosynthesis of lysine and meso-diaminopimelic acid, an essential component of bact

COG id: COG0624

COG function: function code E; Acetylornithine deacetylase/Succinyl-diaminopimelate desuccinylase and related deacylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20A family. DapE subfamily

Homologues:

Organism=Escherichia coli, GI1788816, Length=375, Percent_Identity=40.5333333333333, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI1790395, Length=399, Percent_Identity=26.8170426065163, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DAPE_PHEZH (B4RDU4)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129222.1
- ProteinModelPortal:   B4RDU4
- MEROPS:   M20.010
- GeneID:   6789869
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0379
- HOGENOM:   HBG728841
- OMA:   PMVFEDT
- ProtClustDB:   PRK13009
- GO:   GO:0006508
- HAMAP:   MF_01690
- InterPro:   IPR001261
- InterPro:   IPR005941
- InterPro:   IPR002933
- InterPro:   IPR011650
- TIGRFAMs:   TIGR01246

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20; SSF55031 Peptidase_M20_dimer

EC number: =3.5.1.18

Molecular weight: Translated: 40016; Mature: 39885

Theoretical pI: Translated: 4.95; Mature: 4.95

Prosite motif: PS00758 ARGE_DAPE_CPG2_1; PS00759 ARGE_DAPE_CPG2_2

Important sites: ACT_SITE 71-71 ACT_SITE 135-135

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAAIDAVELSRELIRKPSVTPADEGAMDVVERTLAGLGFACRRMRFGEIENLYARYGTA
CCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPNLCFAGHTDVVPVGDAAAWSKDAFAADVVDGVLIGRGAVDMKSAIAAFAAAAAEAIAA
CCCEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GRVTGSVSFLITGDEEGVATHGTKKVVEALLAEGEAIDHCVVGEPTSAESFGDMVKVGRR
CCEECEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCC
GSINAEILVEGIQGHVAYPHRAANPVPVLVRLLAALQDRALDEGYPEFQPSNLEVTMIDV
CCCCHHEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
PNTATNVIPGTAKARLNIRFNPNHTGQALADWIAAEARKAADGFKGKVTVTPQISGEAFL
CCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEE
TERGPFTELVAAAVKDVTGAEPELSTSGGTSDARFIRALCPVVEVGLVGRTMHQVDERAP
ECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VDEIRRLQAVYAAIIARYFA
HHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAAIDAVELSRELIRKPSVTPADEGAMDVVERTLAGLGFACRRMRFGEIENLYARYGTA
CCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
RPNLCFAGHTDVVPVGDAAAWSKDAFAADVVDGVLIGRGAVDMKSAIAAFAAAAAEAIAA
CCCEEECCCCCEEECCCCCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHH
GRVTGSVSFLITGDEEGVATHGTKKVVEALLAEGEAIDHCVVGEPTSAESFGDMVKVGRR
CCEECEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCCCEEECCCCCCHHHHHHHHHHCCC
GSINAEILVEGIQGHVAYPHRAANPVPVLVRLLAALQDRALDEGYPEFQPSNLEVTMIDV
CCCCHHEEEEECCCCEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEC
PNTATNVIPGTAKARLNIRFNPNHTGQALADWIAAEARKAADGFKGKVTVTPQISGEAFL
CCCCCCCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCEEE
TERGPFTELVAAAVKDVTGAEPELSTSGGTSDARFIRALCPVVEVGLVGRTMHQVDERAP
ECCCCHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VDEIRRLQAVYAAIIARYFA
HHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA