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The map label for this gene is rlmH

Identifier: 197103712

GI number: 197103712

Start: 248367

End: 248831

Strand: Reverse

Name: rlmH

Synonym: PHZ_c0246

Alternate gene names: 197103712

Gene position: 248831-248367 (Counterclockwise)

Preceding gene: 197103713

Following gene: 197103711

Centisome position: 6.23

GC content: 73.98

Gene sequence:

>465_bases
ATGAGGATCAGCATCGTCGCGATCGGCCGCCTCGCGCGCTCGCCCGAGACCGAGCTCGTGAAGCTGTACGTCGAGCGGGC
GACCAACGCCGGCCGCGCGCTGGGCCTCGGCCCTGTCGAGGTGGTGGAGGTCGAGAGCCGCAAGCCGGGCAAGGCGGCCG
AGGCCGAGGCGCTGCGCGCGCACCTCTCCGACGCCCACGTGATCGCCTGCGACGAGCGGGGGGCGGCGCGGCCCTCCCGC
GCCTTCGCCGAGGAGATCGGCCGGCTGCGGGACCAGGGGGTGCGCCGGTTGGTGTTCCTGATCGGCGGCGCCGACGGGCT
CGACCCGGCGCTGGTCGCCCAGGCGAACGGCAAGCTGGCGTTCGGCCCCCAGACCTGGCCCCACGCCCTGGCCCGCGCCA
TGCTGGCCGAGCAGGTCTATCGGGCGGTCTCAATTCTGGCCGGATCGCCGTATCATAGGGACTGA

Upstream 100 bases:

>100_bases
CCGGTCGGCAAGGCCGGCCGCGCGGCCGTCTACTGCCGCTACCACCCCGGGATGACGGCGGCCCTGACGGTCGGCTAGCG
AATGGGGCTATGAACGCCCC

Downstream 100 bases:

>100_bases
TGCGCGGGCGGATCCTGATCTTCGGCGGACTTGCGGCGGCGGCGCTCTCGGCCGTGGCCTTCGCCGCGACCACCGACCAG
CTCACCCGGCTGAACCTGGC

Product: rRNA large subunit methyltransferase

Products: NA

Alternate protein names: 23S rRNA m3Psi1915 methyltransferase; rRNA (pseudouridine-N3-)-methyltransferase rlmH

Number of amino acids: Translated: 154; Mature: 154

Protein sequence:

>154_residues
MRISIVAIGRLARSPETELVKLYVERATNAGRALGLGPVEVVEVESRKPGKAAEAEALRAHLSDAHVIACDERGAARPSR
AFAEEIGRLRDQGVRRLVFLIGGADGLDPALVAQANGKLAFGPQTWPHALARAMLAEQVYRAVSILAGSPYHRD

Sequences:

>Translated_154_residues
MRISIVAIGRLARSPETELVKLYVERATNAGRALGLGPVEVVEVESRKPGKAAEAEALRAHLSDAHVIACDERGAARPSR
AFAEEIGRLRDQGVRRLVFLIGGADGLDPALVAQANGKLAFGPQTWPHALARAMLAEQVYRAVSILAGSPYHRD
>Mature_154_residues
MRISIVAIGRLARSPETELVKLYVERATNAGRALGLGPVEVVEVESRKPGKAAEAEALRAHLSDAHVIACDERGAARPSR
AFAEEIGRLRDQGVRRLVFLIGGADGLDPALVAQANGKLAFGPQTWPHALARAMLAEQVYRAVSILAGSPYHRD

Specific function: Specifically methylates the pseudouridine at position 1915 (m3Psi1915) in 23S rRNA

COG id: COG1576

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase rlmH family

Homologues:

Organism=Escherichia coli, GI1786855, Length=125, Percent_Identity=34.4, Blast_Score=67, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMH_PHEZH (B4RD56)

Other databases:

- EMBL:   CP000747
- RefSeq:   YP_002129089.1
- ProteinModelPortal:   B4RD56
- GeneID:   6789736
- GenomeReviews:   CP000747_GR
- KEGG:   pzu:PHZ_c0246
- HOGENOM:   HBG622807
- OMA:   ILSGHPY
- ProtClustDB:   PRK00103
- GO:   GO:0005737
- HAMAP:   MF_00658
- InterPro:   IPR003742
- PIRSF:   PIRSF004505

Pfam domain/function: PF02590 SPOUT_MTase

EC number: NA

Molecular weight: Translated: 16497; Mature: 16497

Theoretical pI: Translated: 9.68; Mature: 9.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRISIVAIGRLARSPETELVKLYVERATNAGRALGLGPVEVVEVESRKPGKAAEAEALRA
CEEEEEEECHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
HLSDAHVIACDERGAARPSRAFAEEIGRLRDQGVRRLVFLIGGADGLDPALVAQANGKLA
HCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEHEEECCCCCCHHEEEECCCCEE
FGPQTWPHALARAMLAEQVYRAVSILAGSPYHRD
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MRISIVAIGRLARSPETELVKLYVERATNAGRALGLGPVEVVEVESRKPGKAAEAEALRA
CEEEEEEECHHHCCCCHHHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCHHHHHHHHH
HLSDAHVIACDERGAARPSRAFAEEIGRLRDQGVRRLVFLIGGADGLDPALVAQANGKLA
HCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHEEEHEEECCCCCCHHEEEECCCCEE
FGPQTWPHALARAMLAEQVYRAVSILAGSPYHRD
ECCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA