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The map label for this gene is polA [H]

Identifier: 197103637

GI number: 197103637

Start: 166671

End: 167288

Strand: Direct

Name: polA [H]

Synonym: PHZ_c0171

Alternate gene names: 197103637

Gene position: 166671-167288 (Clockwise)

Preceding gene: 197103636

Following gene: 197103638

Centisome position: 4.17

GC content: 68.28

Gene sequence:

>618_bases
GTGACCACTTTCGTTCATGAGGGGGACCTGCCCGCCGACGTGCTGGCCAGCGCCTCGTCCGTCGCCATCGATTCCGAGAC
CATGGGCCTGCGCCTGGGCCGCGACCCCTTGTGCGTCGTGCAGCTCTCGGACGGCGGCGGCGACGCCCACCTCGTGAAGC
TGGACCGGACCACCTACGACGCGCCGAACCTGAAGCGGCTGCTGACCGATCCGGCGGTGACCAAGATCTTCCACTTCGGC
CGCTTCGACATCGCCATGTTCTGGCTGCACCTGGGCGTGCTGACGGCCCCGGTCTACTGCACCAAGATCGCCTCGAAGAT
CGCCCGCACCTACACCGACCGGCACGGCCTGAAGGACATCTCCAAGGAGCTGCTGGGCGTCGACATGTCCAAGGCCCAGC
AGAGCTCGGACTGGGGCGCGGGCAAGCTCTCGGACGAACAGGTCGCCTACGCCGCCTCGGACGTGCTGCACCTGCACGCC
CTGAAGGACCGGCTGCAGCAGATGCTGGTCCGCGAGGGCCGCGACAGCCTGGCCCAGGCCTGCTTCGACTTCCTGCCGCA
CCGCGCCGTCCTCGACGTCGCCGGGTGGGAGGACGTCGACATCTTCGCCCATTCCTAG

Upstream 100 bases:

>100_bases
CCTGTCGTCCCGGCCGGCGCGGAAACGCCATGGAATTCAACGCCCATCGCGCGACACTCGGAAAGCGCGGGGCGGGCCGA
TGTCGGAGTCTCAAGGCGAC

Downstream 100 bases:

>100_bases
AGGCCGGCCATGACCGCGTCCCACGCCTCGATCGACGCCACGCCTCCCCGGCGCGCCGACTTCGAGCGCTGGCGGCGGCG
CTCGAAGCTGATCCGGCTGC

Product: ribonuclease D

Products: NA

Alternate protein names: POL I [H]

Number of amino acids: Translated: 205; Mature: 204

Protein sequence:

>205_residues
MTTFVHEGDLPADVLASASSVAIDSETMGLRLGRDPLCVVQLSDGGGDAHLVKLDRTTYDAPNLKRLLTDPAVTKIFHFG
RFDIAMFWLHLGVLTAPVYCTKIASKIARTYTDRHGLKDISKELLGVDMSKAQQSSDWGAGKLSDEQVAYAASDVLHLHA
LKDRLQQMLVREGRDSLAQACFDFLPHRAVLDVAGWEDVDIFAHS

Sequences:

>Translated_205_residues
MTTFVHEGDLPADVLASASSVAIDSETMGLRLGRDPLCVVQLSDGGGDAHLVKLDRTTYDAPNLKRLLTDPAVTKIFHFG
RFDIAMFWLHLGVLTAPVYCTKIASKIARTYTDRHGLKDISKELLGVDMSKAQQSSDWGAGKLSDEQVAYAASDVLHLHA
LKDRLQQMLVREGRDSLAQACFDFLPHRAVLDVAGWEDVDIFAHS
>Mature_204_residues
TTFVHEGDLPADVLASASSVAIDSETMGLRLGRDPLCVVQLSDGGGDAHLVKLDRTTYDAPNLKRLLTDPAVTKIFHFGR
FDIAMFWLHLGVLTAPVYCTKIASKIARTYTDRHGLKDISKELLGVDMSKAQQSSDWGAGKLSDEQVAYAASDVLHLHAL
KDRLQQMLVREGRDSLAQACFDFLPHRAVLDVAGWEDVDIFAHS

Specific function: Cleaves Multimeric tRNA Precursor At The Spacer Region. [C]

COG id: COG0349

COG function: function code J; Ribonuclease D

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 3'-5' exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1788105, Length=148, Percent_Identity=32.4324324324324, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002562
- InterPro:   IPR019760
- InterPro:   IPR001098
- InterPro:   IPR002298
- InterPro:   IPR012337 [H]

Pfam domain/function: PF01612 3_5_exonuc; PF00476 DNA_pol_A [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 22521; Mature: 22390

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTFVHEGDLPADVLASASSVAIDSETMGLRLGRDPLCVVQLSDGGGDAHLVKLDRTTYD
CCCCCCCCCCCHHHHHCCCHHEECCHHCCEEECCCCEEEEEECCCCCCEEEEEEECCCCC
APNLKRLLTDPAVTKIFHFGRFDIAMFWLHLGVLTAPVYCTKIASKIARTYTDRHGLKDI
CCCHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SKELLGVDMSKAQQSSDWGAGKLSDEQVAYAASDVLHLHALKDRLQQMLVREGRDSLAQA
HHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
CFDFLPHRAVLDVAGWEDVDIFAHS
HHHHCCCHHEEECCCCCCCEEEECC
>Mature Secondary Structure 
TTFVHEGDLPADVLASASSVAIDSETMGLRLGRDPLCVVQLSDGGGDAHLVKLDRTTYD
CCCCCCCCCCHHHHHCCCHHEECCHHCCEEECCCCEEEEEECCCCCCEEEEEEECCCCC
APNLKRLLTDPAVTKIFHFGRFDIAMFWLHLGVLTAPVYCTKIASKIARTYTDRHGLKDI
CCCHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHH
SKELLGVDMSKAQQSSDWGAGKLSDEQVAYAASDVLHLHALKDRLQQMLVREGRDSLAQA
HHHHHCCCHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
CFDFLPHRAVLDVAGWEDVDIFAHS
HHHHCCCHHEEECCCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]