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The map label for this gene is sucB [H]

Identifier: 197103627

GI number: 197103627

Start: 152118

End: 153398

Strand: Direct

Name: sucB [H]

Synonym: PHZ_c0161

Alternate gene names: 197103627

Gene position: 152118-153398 (Clockwise)

Preceding gene: 197103626

Following gene: 197103629

Centisome position: 3.81

GC content: 70.49

Gene sequence:

>1281_bases
ATGGCCGACATCATGACGCCAGCGCTGGGCGAATCCGTCACCGAAGCGACGGTGGCGCGCTGGACGAAGAAGGCCGGTGA
CGCCGTCCGCAAGGACGAGATCCTCGTCGAGCTCGAGACGGACAAGGTGAGCCTGGAAGTGGCCGCCCCCGCCGACGGCG
TGCTGGCCGAGATCGCCGCCGACGAAGGCGCGACGGTCGAGCCCGGCGCCGTGCTGGGCCGCATCACCGAAGGCGCCGGC
GCCCCGGCGCCGAAGGCCGAGGCGCCCAAGGCGGCCGCCCCGTCGTCCACGCCGACGCCGGTCCCGGCCGGCGAGCTGCA
GCCCGAGCCGACCCCCGGCAAGGCCGTCCCGACCTCGGCCCCGGTGCCGGATACGTCGGCTCCCCAGGGAGGGGCCAAGG
CCGAGAAGGTGCTGGCGCCGTCGGCCCAGCGGATCGTCGGCGAGAACAAGCTGGACGCCGGCGCCATTCAGGGCACCGGC
AAGGACGGCCGGGTGACCAAGGCCGACGCCCTCGCCGCGCTGGAGTCCCGCGCCAAGGCGCCGGCCGCCCCGCAGGCACC
GTCCGCGCCGCGCGAGCTGCACGAGCGCGAGGAGCGGGTCCGCATGACCCGCCTGCGCCAGACCATCGCGCGCCGGCTGA
AGGAGGCGCAGAACGCCGCGGCCATGCTGACCACCTTCAACGAGGTGGACATGACGAACGTCATGGCCCTGCGGAACCAG
TACAAGGACCAGTTCGAGAAGAAGCACGGCGTGAAGCTGGGCTTCATGAGCTTCTTCGTCCGCGCGGTGATCCACGGCCT
GAAGCAGGTGCCCGAGGTCAACGCCGAGATCGACGGCACGGACATCATCTACAAGAACCACTACGACATCTCGGTCGCGG
TGGGCACCGACAAGGGCCTCGTGACGCCCGTCGTCCGCGACGCCGACATGATGAGCCTGGCCGAGATCGAGAAGGAGATC
GGCGCGCTCGGCAAGAAGGCCCGCGACGGCCAGCTGGCGCTGGAGGACCTGCAGGGCGGCACGTTCACCATCTCGAACGG
CGGCGTGTACGGCTCGCTGATGTCGACGCCGATCCTGAACGCCCCGCAGTCGGGCATCCTCGGCATGCACAAGATCCAGG
AGCGGCCGATGGTGGTCGGCGGCCAGATCGTCGCCCGGCCGATGATGTACCTTGCGCTCAGCTACGATCACCGCATCGTC
GACGGCAAGGGCGCGGTGACCTTCCTGGTCCACGTCAAGGAAGCCATCGAGGACCCGCAGCGCCTGCTGCTGGACGTCTG
A

Upstream 100 bases:

>100_bases
GGAGCTCGAGGCGTTCCTCACGGAAGCCTTCGCCTGAACCCCATTTCCCTGACCCGCACTCTGTTTGTGTCCGAGACCCG
ATAAGGAGCGAAATCCCCAA

Downstream 100 bases:

>100_bases
GCGACACGCCTTCTTCCCTCGGGAGAGAAGGTGTCGCCGGAAGAACGACGGATGAGGGGCCGCATCGCGAGTGCGGCCCC
TCTTTCGTCCTGAAGGGCCG

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 426; Mature: 425

Protein sequence:

>426_residues
MADIMTPALGESVTEATVARWTKKAGDAVRKDEILVELETDKVSLEVAAPADGVLAEIAADEGATVEPGAVLGRITEGAG
APAPKAEAPKAAAPSSTPTPVPAGELQPEPTPGKAVPTSAPVPDTSAPQGGAKAEKVLAPSAQRIVGENKLDAGAIQGTG
KDGRVTKADALAALESRAKAPAAPQAPSAPRELHEREERVRMTRLRQTIARRLKEAQNAAAMLTTFNEVDMTNVMALRNQ
YKDQFEKKHGVKLGFMSFFVRAVIHGLKQVPEVNAEIDGTDIIYKNHYDISVAVGTDKGLVTPVVRDADMMSLAEIEKEI
GALGKKARDGQLALEDLQGGTFTISNGGVYGSLMSTPILNAPQSGILGMHKIQERPMVVGGQIVARPMMYLALSYDHRIV
DGKGAVTFLVHVKEAIEDPQRLLLDV

Sequences:

>Translated_426_residues
MADIMTPALGESVTEATVARWTKKAGDAVRKDEILVELETDKVSLEVAAPADGVLAEIAADEGATVEPGAVLGRITEGAG
APAPKAEAPKAAAPSSTPTPVPAGELQPEPTPGKAVPTSAPVPDTSAPQGGAKAEKVLAPSAQRIVGENKLDAGAIQGTG
KDGRVTKADALAALESRAKAPAAPQAPSAPRELHEREERVRMTRLRQTIARRLKEAQNAAAMLTTFNEVDMTNVMALRNQ
YKDQFEKKHGVKLGFMSFFVRAVIHGLKQVPEVNAEIDGTDIIYKNHYDISVAVGTDKGLVTPVVRDADMMSLAEIEKEI
GALGKKARDGQLALEDLQGGTFTISNGGVYGSLMSTPILNAPQSGILGMHKIQERPMVVGGQIVARPMMYLALSYDHRIV
DGKGAVTFLVHVKEAIEDPQRLLLDV
>Mature_425_residues
ADIMTPALGESVTEATVARWTKKAGDAVRKDEILVELETDKVSLEVAAPADGVLAEIAADEGATVEPGAVLGRITEGAGA
PAPKAEAPKAAAPSSTPTPVPAGELQPEPTPGKAVPTSAPVPDTSAPQGGAKAEKVLAPSAQRIVGENKLDAGAIQGTGK
DGRVTKADALAALESRAKAPAAPQAPSAPRELHEREERVRMTRLRQTIARRLKEAQNAAAMLTTFNEVDMTNVMALRNQY
KDQFEKKHGVKLGFMSFFVRAVIHGLKQVPEVNAEIDGTDIIYKNHYDISVAVGTDKGLVTPVVRDADMMSLAEIEKEIG
ALGKKARDGQLALEDLQGGTFTISNGGVYGSLMSTPILNAPQSGILGMHKIQERPMVVGGQIVARPMMYLALSYDHRIVD
GKGAVTFLVHVKEAIEDPQRLLLDV

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:2-oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2) and l

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI19923748, Length=232, Percent_Identity=58.1896551724138, Blast_Score=294, Evalue=8e-80,
Organism=Homo sapiens, GI203098753, Length=460, Percent_Identity=31.0869565217391, Blast_Score=181, Evalue=1e-45,
Organism=Homo sapiens, GI203098816, Length=460, Percent_Identity=30.8695652173913, Blast_Score=179, Evalue=3e-45,
Organism=Homo sapiens, GI31711992, Length=452, Percent_Identity=29.8672566371681, Blast_Score=173, Evalue=3e-43,
Organism=Homo sapiens, GI110671329, Length=440, Percent_Identity=27.9545454545455, Blast_Score=156, Evalue=3e-38,
Organism=Homo sapiens, GI260898739, Length=167, Percent_Identity=38.3233532934132, Blast_Score=112, Evalue=9e-25,
Organism=Escherichia coli, GI1786946, Length=427, Percent_Identity=51.9906323185012, Blast_Score=421, Evalue=1e-119,
Organism=Escherichia coli, GI1786305, Length=300, Percent_Identity=32, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25146366, Length=442, Percent_Identity=42.7601809954751, Blast_Score=325, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17560088, Length=445, Percent_Identity=31.4606741573034, Blast_Score=191, Evalue=6e-49,
Organism=Caenorhabditis elegans, GI17537937, Length=433, Percent_Identity=27.7136258660508, Blast_Score=157, Evalue=1e-38,
Organism=Caenorhabditis elegans, GI17538894, Length=314, Percent_Identity=28.6624203821656, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320352, Length=421, Percent_Identity=45.3681710213777, Blast_Score=344, Evalue=1e-95,
Organism=Saccharomyces cerevisiae, GI6324258, Length=457, Percent_Identity=30.1969365426696, Blast_Score=162, Evalue=1e-40,
Organism=Drosophila melanogaster, GI24645909, Length=223, Percent_Identity=58.2959641255605, Blast_Score=280, Evalue=1e-75,
Organism=Drosophila melanogaster, GI18859875, Length=439, Percent_Identity=29.1571753986333, Blast_Score=160, Evalue=2e-39,
Organism=Drosophila melanogaster, GI20129315, Length=237, Percent_Identity=33.7552742616034, Blast_Score=134, Evalue=1e-31,
Organism=Drosophila melanogaster, GI24582497, Length=237, Percent_Identity=33.7552742616034, Blast_Score=134, Evalue=2e-31,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 45029; Mature: 44898

Theoretical pI: Translated: 5.75; Mature: 5.75

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MADIMTPALGESVTEATVARWTKKAGDAVRKDEILVELETDKVSLEVAAPADGVLAEIAA
CCCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHC
DEGATVEPGAVLGRITEGAGAPAPKAEAPKAAAPSSTPTPVPAGELQPEPTPGKAVPTSA
CCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PVPDTSAPQGGAKAEKVLAPSAQRIVGENKLDAGAIQGTGKDGRVTKADALAALESRAKA
CCCCCCCCCCCCHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCC
PAAPQAPSAPRELHEREERVRMTRLRQTIARRLKEAQNAAAMLTTFNEVDMTNVMALRNQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
YKDQFEKKHGVKLGFMSFFVRAVIHGLKQVPEVNAEIDGTDIIYKNHYDISVAVGTDKGL
HHHHHHHHHCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCC
VTPVVRDADMMSLAEIEKEIGALGKKARDGQLALEDLQGGTFTISNGGVYGSLMSTPILN
CCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHCCCCEEEEECCCEEEHHHHCCCCC
APQSGILGMHKIQERPMVVGGQIVARPMMYLALSYDHRIVDGKGAVTFLVHVKEAIEDPQ
CCCCCCCCHHHHHCCCEEECCHHHHHHHHHHHCCCCCEEECCCCCEEHHEEHHHHHCCHH
RLLLDV
HHHCCC
>Mature Secondary Structure 
ADIMTPALGESVTEATVARWTKKAGDAVRKDEILVELETDKVSLEVAAPADGVLAEIAA
CCCCCCHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEECCEEEEEEECCCCCHHHHHHC
DEGATVEPGAVLGRITEGAGAPAPKAEAPKAAAPSSTPTPVPAGELQPEPTPGKAVPTSA
CCCCCCCCCHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PVPDTSAPQGGAKAEKVLAPSAQRIVGENKLDAGAIQGTGKDGRVTKADALAALESRAKA
CCCCCCCCCCCCHHHHHHCCCHHHHHCCCCCCCCCCCCCCCCCCEEHHHHHHHHHHHCCC
PAAPQAPSAPRELHEREERVRMTRLRQTIARRLKEAQNAAAMLTTFNEVDMTNVMALRNQ
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHH
YKDQFEKKHGVKLGFMSFFVRAVIHGLKQVPEVNAEIDGTDIIYKNHYDISVAVGTDKGL
HHHHHHHHHCCEEHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCEEEEEEECCCCC
VTPVVRDADMMSLAEIEKEIGALGKKARDGQLALEDLQGGTFTISNGGVYGSLMSTPILN
CCHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEHHHCCCCEEEEECCCEEEHHHHCCCCC
APQSGILGMHKIQERPMVVGGQIVARPMMYLALSYDHRIVDGKGAVTFLVHVKEAIEDPQ
CCCCCCCCHHHHHCCCEEECCHHHHHHHHHHHCCCCCEEECCCCCEEHHEEHHHHHCCHH
RLLLDV
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12874367 [H]