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The map label for this gene is ogt [H]

Identifier: 197103585

GI number: 197103585

Start: 108881

End: 109444

Strand: Direct

Name: ogt [H]

Synonym: PHZ_c0119

Alternate gene names: 197103585

Gene position: 108881-109444 (Clockwise)

Preceding gene: 197103584

Following gene: 197103587

Centisome position: 2.72

GC content: 74.47

Gene sequence:

>564_bases
ATGATCCGGTTCGCACTCTTCGAGACGGCCATCGGCTGGGCGGGGATCGCCTGGAACGAGGCGGACAGCCAGGCGCGCCT
CGTCGCCTGCCACCTGCCCGAACGCGACGCCGAGACGGCGCGGCGCGGCTTCCGGCGCCGCTTCCCCGAGACGGCCGAGG
CCGAGCCGCCGGCGTCGCTGTCGCCGACGATCGCCGGGATCCAGGCGCTGATGCGCGGCGAGAAGGCCGACCTGTCGGCC
GCGCCGCTGGACCTGTCGCGCACGCCCCCGTTCCACGCCCGCGTCTACGAGATCGCCCGCGCGATCCCGCCGGGCGAGAC
CCTGACCTACGGCGAGATCGCCGAGAAGCTGGGCGACAAGCTGCTGGCCCGCGACGTCGGCCAGGCGCTCGGCAAGAACC
CCTGGCCGATCGTGGTGCCGTGCCACCGGGTCACCGCCGCCGGCGGCAAGCCCGGCGGCTTCTCGGCCCGCGGCGGGGTG
GACACCAAGCTGAGGCTGCTCGCCATCGAGGGCGCGAAGGCGGCCATCCCGAAGGAGACTCCCCAGGGCGACCTGTTCGC
CTGA

Upstream 100 bases:

>100_bases
GCGGCTCGTCCAGCATCAGCAGACGCCGCTCCAGGGGACCTCGACCTAGCAAGTCCGAAAGCCGCCAGCGGCTATCGGCG
ACCGGCGCTATATTGCGGTC

Downstream 100 bases:

>100_bases
GGGTCAGCTCGTCGGGAAGCCCTTGTCCTTCAGGTAGGCCTTCACGTCGGGGTCGCGGCCGCGGAAGGCGCGGTAGCCCT
CGCGCGGGTGGACCGAGTTG

Product: putative methylated-DNA-protein-cystein methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 187; Mature: 187

Protein sequence:

>187_residues
MIRFALFETAIGWAGIAWNEADSQARLVACHLPERDAETARRGFRRRFPETAEAEPPASLSPTIAGIQALMRGEKADLSA
APLDLSRTPPFHARVYEIARAIPPGETLTYGEIAEKLGDKLLARDVGQALGKNPWPIVVPCHRVTAAGGKPGGFSARGGV
DTKLRLLAIEGAKAAIPKETPQGDLFA

Sequences:

>Translated_187_residues
MIRFALFETAIGWAGIAWNEADSQARLVACHLPERDAETARRGFRRRFPETAEAEPPASLSPTIAGIQALMRGEKADLSA
APLDLSRTPPFHARVYEIARAIPPGETLTYGEIAEKLGDKLLARDVGQALGKNPWPIVVPCHRVTAAGGKPGGFSARGGV
DTKLRLLAIEGAKAAIPKETPQGDLFA
>Mature_187_residues
MIRFALFETAIGWAGIAWNEADSQARLVACHLPERDAETARRGFRRRFPETAEAEPPASLSPTIAGIQALMRGEKADLSA
APLDLSRTPPFHARVYEIARAIPPGETLTYGEIAEKLGDKLLARDVGQALGKNPWPIVVPCHRVTAAGGKPGGFSARGGV
DTKLRLLAIEGAKAAIPKETPQGDLFA

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Escherichia coli, GI1787596, Length=157, Percent_Identity=35.6687898089172, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI1788542, Length=170, Percent_Identity=29.4117647058824, Blast_Score=64, Evalue=9e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1 [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19987; Mature: 19987

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIRFALFETAIGWAGIAWNEADSQARLVACHLPERDAETARRGFRRRFPETAEAEPPASL
CEEHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCC
SPTIAGIQALMRGEKADLSAAPLDLSRTPPFHARVYEIARAIPPGETLTYGEIAEKLGDK
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
LLARDVGQALGKNPWPIVVPCHRVTAAGGKPGGFSARGGVDTKLRLLAIEGAKAAIPKET
HHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
PQGDLFA
CCCCCCC
>Mature Secondary Structure
MIRFALFETAIGWAGIAWNEADSQARLVACHLPERDAETARRGFRRRFPETAEAEPPASL
CEEHHHHHHHHCCCCEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCC
SPTIAGIQALMRGEKADLSAAPLDLSRTPPFHARVYEIARAIPPGETLTYGEIAEKLGDK
CHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCEEHHHHHHHHHHH
LLARDVGQALGKNPWPIVVPCHRVTAAGGKPGGFSARGGVDTKLRLLAIEGAKAAIPKET
HHHHHHHHHHCCCCCEEEEECCEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCC
PQGDLFA
CCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9371463 [H]