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The map label for this gene is estA [C]

Identifier: 197103562

GI number: 197103562

Start: 89525

End: 90649

Strand: Direct

Name: estA [C]

Synonym: PHZ_c0096

Alternate gene names: 197103562

Gene position: 89525-90649 (Clockwise)

Preceding gene: 197103561

Following gene: 197103563

Centisome position: 2.24

GC content: 72.89

Gene sequence:

>1125_bases
ATGGTCGAGATTGCAGGCTTCGCGCCGCCCCGGTTCGCCGCCGTCAAGGACGCCTTCGCCGCCCACTTCGAGGCGGGCGA
GGAGCTCGGGGCGCGGTTCACCCTCGTCGAGGCGGGCGAGGTCGTCCTCGACCTGTGGGCGGGCCATGCCGACCGCAAGC
GGACGCGGGCCTTCGATGAGCAGACCCTGACCTGCATCTTCTCGACGACCAAGGCCCTGGCCGCCCTGCTGGTGGCGCGG
GCGGTGGAACGCGGCCTCCTCCGCTACGACCAGCCCGTGGCCGAGGTCTGGCCCGAGTTCGCGCAAGCCGGAAAGGCGGC
GATCACCGTCGAGCAGGTCCTGTCGCACCAGGCGGGCCTGTCGGGGTTCCTGGAGCCCATGGAGCCCAGCCTGTGGGTCG
AGTGGGACGCCATCGCCGACAAGCTCGCCGCCATGGCGCCGATGTGGCCGCCGGGAACGGCCAGCGGCTACCATCCGATC
ACCTTCGGCTACCTGGCCGGCGAGGTCTTCCGCCGGACCGACGGGCGCATGATGGGCGACGCCCTGGGGGCGGATCTTCG
GGCCCCCTTCGGCCTCGACCTCTGGATCGGCCTGCCTGAGAGCGAGTTCGGCCGCCTGGCCGAGCTGCAGCGGCCCAGCG
CCCTGCCGGACTTCGGCGAGGTGAACGATCCCACCCGCGCCGCCTTCCTGACGCCCTGGGCCTCGGTCGGCGGCGTTCCG
GCCGCTGTCTGGCGCAAGACACAGGTCCCGTCGGCCAACGGCTACGCCACGGCGCTCGCCCTCGCGCGGCTGATGGGCGC
GCTGGCCGACAACGGCTGGCTCGAGGGCGGGCAGATCCTGTCGCCCGCCCTGATCGCCGAGGCGAGCCGCGAGCGCATCC
ACGGCCAGGACCTGGTGCTGCCGTTCGAGATGAGCTGGGGCGCGGGCTTCATGCGCAATCCGCCGAACAATCCCTGGGGC
CCGGGCGAGCGGACCTTCGGCCACTCGGGCTGGGGCGGGAGCTGCGCCTTCGCCGATCCGGACCGTCGACTGGGCGGCGC
CTATGTGATGAACAAGCAGGGGACCGCCCTGATGGGCGACGCCCGGCCCCGGCGGCTGATCGAGGCCGCCTACGGGGCGC
TCTGA

Upstream 100 bases:

>100_bases
CCTGCGCCGACGGGCCGCCGGCCGCGCGGGTCACGGCGGTCACGCCGGCCGAGGCGGCGGACGACGGCGCCGAGGGTTTC
GAGGAAAGGGCCACGGTCTG

Downstream 100 bases:

>100_bases
GCGCCATGTCGGACATCAAGCTGGATTTCCGCGCCGCGGGACCTACGGACCTTCCCGCTGTCCACCGGCTGGTGGAGAGC
GCCTATCGCGGCGACAGCGC

Product: esterase A

Products: A Substituted Beta- Amino Acid. [C]

Alternate protein names: Esterase; Carboxylesterase; Beta-Lactamase Class C; Esterase/Lipase LipP; Esterase A; Esterase/Lipase/Beta-Lactamase; Lactone Hydrolase; Lipase LipE; Esterase LipL; Lipase LipD; Lipase/Esterase/Beta-Lactamase; Fusion Of Enoyl-CoA Hydratase; Beta-Lactamase-Like Protein; Beta-Lactamase Protein; Carboxylesterase EstC; Serine Hydrolase; Beta-Lactamase Family

Number of amino acids: Translated: 374; Mature: 374

Protein sequence:

>374_residues
MVEIAGFAPPRFAAVKDAFAAHFEAGEELGARFTLVEAGEVVLDLWAGHADRKRTRAFDEQTLTCIFSTTKALAALLVAR
AVERGLLRYDQPVAEVWPEFAQAGKAAITVEQVLSHQAGLSGFLEPMEPSLWVEWDAIADKLAAMAPMWPPGTASGYHPI
TFGYLAGEVFRRTDGRMMGDALGADLRAPFGLDLWIGLPESEFGRLAELQRPSALPDFGEVNDPTRAAFLTPWASVGGVP
AAVWRKTQVPSANGYATALALARLMGALADNGWLEGGQILSPALIAEASRERIHGQDLVLPFEMSWGAGFMRNPPNNPWG
PGERTFGHSGWGGSCAFADPDRRLGGAYVMNKQGTALMGDARPRRLIEAAYGAL

Sequences:

>Translated_374_residues
MVEIAGFAPPRFAAVKDAFAAHFEAGEELGARFTLVEAGEVVLDLWAGHADRKRTRAFDEQTLTCIFSTTKALAALLVAR
AVERGLLRYDQPVAEVWPEFAQAGKAAITVEQVLSHQAGLSGFLEPMEPSLWVEWDAIADKLAAMAPMWPPGTASGYHPI
TFGYLAGEVFRRTDGRMMGDALGADLRAPFGLDLWIGLPESEFGRLAELQRPSALPDFGEVNDPTRAAFLTPWASVGGVP
AAVWRKTQVPSANGYATALALARLMGALADNGWLEGGQILSPALIAEASRERIHGQDLVLPFEMSWGAGFMRNPPNNPWG
PGERTFGHSGWGGSCAFADPDRRLGGAYVMNKQGTALMGDARPRRLIEAAYGAL
>Mature_374_residues
MVEIAGFAPPRFAAVKDAFAAHFEAGEELGARFTLVEAGEVVLDLWAGHADRKRTRAFDEQTLTCIFSTTKALAALLVAR
AVERGLLRYDQPVAEVWPEFAQAGKAAITVEQVLSHQAGLSGFLEPMEPSLWVEWDAIADKLAAMAPMWPPGTASGYHPI
TFGYLAGEVFRRTDGRMMGDALGADLRAPFGLDLWIGLPESEFGRLAELQRPSALPDFGEVNDPTRAAFLTPWASVGGVP
AAVWRKTQVPSANGYATALALARLMGALADNGWLEGGQILSPALIAEASRERIHGQDLVLPFEMSWGAGFMRNPPNNPWG
PGERTFGHSGWGGSCAFADPDRRLGGAYVMNKQGTALMGDARPRRLIEAAYGAL

Specific function: This Protein Is A Serine Beta-Lactamase With A Substrate Specificity For Cephalosporins. [C]

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Caenorhabditis elegans, GI17538001, Length=399, Percent_Identity=31.8295739348371, Blast_Score=174, Evalue=6e-44,
Organism=Caenorhabditis elegans, GI17535191, Length=381, Percent_Identity=31.758530183727, Blast_Score=173, Evalue=1e-43,
Organism=Caenorhabditis elegans, GI71983659, Length=372, Percent_Identity=31.4516129032258, Blast_Score=164, Evalue=7e-41,
Organism=Caenorhabditis elegans, GI17538474, Length=404, Percent_Identity=30.1980198019802, Blast_Score=162, Evalue=3e-40,
Organism=Caenorhabditis elegans, GI17535177, Length=397, Percent_Identity=27.9596977329975, Blast_Score=157, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI71983666, Length=287, Percent_Identity=32.7526132404181, Blast_Score=152, Evalue=2e-37,
Organism=Caenorhabditis elegans, GI71990590, Length=396, Percent_Identity=28.030303030303, Blast_Score=135, Evalue=5e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 3.5.2.6 [C]

Molecular weight: Translated: 40218; Mature: 40218

Theoretical pI: Translated: 5.00; Mature: 5.00

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVEIAGFAPPRFAAVKDAFAAHFEAGEELGARFTLVEAGEVVLDLWAGHADRKRTRAFDE
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHCCCCHHHHHHHHHH
QTLTCIFSTTKALAALLVARAVERGLLRYDQPVAEVWPEFAQAGKAAITVEQVLSHQAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHCH
SGFLEPMEPSLWVEWDAIADKLAAMAPMWPPGTASGYHPITFGYLAGEVFRRTDGRMMGD
HHCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
ALGADLRAPFGLDLWIGLPESEFGRLAELQRPSALPDFGEVNDPTRAAFLTPWASVGGVP
HHCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEECCHHHHCCCC
AAVWRKTQVPSANGYATALALARLMGALADNGWLEGGQILSPALIAEASRERIHGQDLVL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCHHHHHHHHHHHCCCCCEEE
PFEMSWGAGFMRNPPNNPWGPGERTFGHSGWGGSCAFADPDRRLGGAYVMNKQGTALMGD
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCCCEEECC
ARPRRLIEAAYGAL
CCHHHHHHHHHCCC
>Mature Secondary Structure
MVEIAGFAPPRFAAVKDAFAAHFEAGEELGARFTLVEAGEVVLDLWAGHADRKRTRAFDE
CEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEEECCHHHHHHHCCCCHHHHHHHHHH
QTLTCIFSTTKALAALLVARAVERGLLRYDQPVAEVWPEFAQAGKAAITVEQVLSHQAGL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCEEEHHHHHHHHHCH
SGFLEPMEPSLWVEWDAIADKLAAMAPMWPPGTASGYHPITFGYLAGEVFRRTDGRMMGD
HHCCCCCCCCEEEEHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHH
ALGADLRAPFGLDLWIGLPESEFGRLAELQRPSALPDFGEVNDPTRAAFLTPWASVGGVP
HHCCCCCCCCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCCCCHHEEECCHHHHCCCC
AAVWRKTQVPSANGYATALALARLMGALADNGWLEGGQILSPALIAEASRERIHGQDLVL
HHHHHCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCHHCHHHHHHHHHHHCCCCCEEE
PFEMSWGAGFMRNPPNNPWGPGERTFGHSGWGGSCAFADPDRRLGGAYVMNKQGTALMGD
EEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHCCCEEEECCCCCEEECC
ARPRRLIEAAYGAL
CCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: A Beta-Lactam; H2O [C]

Specific reaction: A Beta-Lactam + H2O = A Substituted Beta- Amino Acid. [C]

General reaction: Carboxylic acid amide hydrolysis [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA