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The map label for this gene is emrB [H]

Identifier: 197103490

GI number: 197103490

Start: 20595

End: 22223

Strand: Direct

Name: emrB [H]

Synonym: PHZ_c0024

Alternate gene names: 197103490

Gene position: 20595-22223 (Clockwise)

Preceding gene: 197103489

Following gene: 197103491

Centisome position: 0.52

GC content: 68.14

Gene sequence:

>1629_bases
GTGACCGAAGCCGCCATCCATCCCGACAGCGGCCAGGCCAAGGCCGCCGCCCAGCCGCACGCCTACATGGGCGCGGGCGG
CGAGGCCGTGGACTGGATGAAGATCTTCCTGGGCTTCGGCGGGATGGTCATCGGCCAGTTCATGGCCATGCTGGACATCC
AGATCGTGGCCAGCTCGCTGGTCCAGATCCAGGCCGGCATCGGCGCGACCGCCGACGAGATCAGCTGGGTGCAGACCATC
TACCTCCTGGCCGAGGTGGTGATCATGCCGCTCACCGCGTACCTGACGAAGATGTGGGGCACGCGGTCGTTCTACGTGAT
CGCGGTCGTCGGCTTCATCGTCACCTCGGTCGCGGTGGGCCTGTCGTCCAGCGTCGGAATGATGATCTTCTTCCGGGCGC
TGCAGGGCGTGTTCGCCGGCGCGATGATCCCGCCGATCTTCGCCACGGCCATGACCGTATTCCCGCCCGACAAGCGGATC
ACCGCCAACGTCATCGTGGGCCTGATCGTCACTCTCGCGCCGACCGTTGGGCCGACCTTGGGCGGCCACCTGACCGAGGC
GCTGAACTGGCGCTGGCTGTTCTTCATCAACGTGCCGGTGGGCGCGCTGGTGATCTTCCTGGTCGGCCGCTACGGCGACT
TCGACAAGGGCGACCCCAGCCTCTCGAAGGGCATCGACTGGTGGGGCCTGGGCCTGATGACGGTCTTCCTGCTCTCGATG
CAGTACGTGCTCGAAGAGGGGAACGACGAGGGCTGGTTCGAGGCGGACCTGATCCTGTGGCTGACGGTGACCGCGGCGCT
CACGGGCGTGGCGTTCATCTGGCGTCAGCTCGCCTACCGGCAGCCGATCGTGTCGCTGACCCCGTTCCGGGACCGCAACT
TCACCCTCGGGATCATCATGACCTTCATCTCGGGGGCCAGCCTGTTCGGGGGCACCTTCCTGCTGCCGATCTTCCTGGGA
CAGATCCGCGGCTTCTCCTCGGCCGAGGTCGGCACGACGATGCTGGTCTCGGGCCTGTCGATGTTCCTGAGCGCGCCGCT
GGCGGGACGGCTGGTGCGGATGGTGGACGCCCGGATATCCATGGTCGTCGGATTCGCCCTGGCGGCCTGGGCGATCCAGC
TCGGCGTCCGGGTCACAGACGAATGGGGGTTCCGTGAGTTTTTCGTGCTGCAGGCGGTGCGCGGGTTCGGCACCATGATC
GCGATGATCGCCTCGCAGCAGATGGCCGTCTCCACCCTGCCGGTCAGCGCCATGAAGGACGCCTCGGGCCTCATCAACCT
GGTGCGCAACGTGGCCGGCGCGATCGGCCTGGCGCTCCTGTCGACCATCCTCACCCACCAGGCGGCGGTGCACTACGCCG
ACCTGGCCTCGGCGGTCAGCATCGCCAACCCCCAGAGCACGGCCATGTTCGACGGCCTCGTCCAGATGATGACCGCCGGC
GGCATGGCCGATCCCGAGGGCGGGGCGCGCAAGGCCTTCAGCATGATGATGCACCGGCAGGCCTCGACCCTCAGCTTCGG
CGACGCCTTCGGCGTGCTGGCGATGGGCTGCTGGGTCGCCGCAGCCCTGGCGTTCTTCGCCCGGCCCGGGCGGCCGGGCG
CGGCCCCGGCCAACACGGAGGCGCACTGA

Upstream 100 bases:

>100_bases
AAGGTCGATGTGACCGAGAAGACCGGCCCCTCCTTCGCCGAGGCCGGCGAGATCCCCGCCCGCTACGCCCGCGAGGGCGC
CTCGCGCTGAGCTGCGGGTC

Downstream 100 bases:

>100_bases
TGGCGCGCGTCACGGGCCAGATCGACGTCGCGAAGAGCGAGGCCATCCTGGATGCAGCCCTCGACGTCTTCGTCGAGCGG
GGCGTCTCGGCGGCGATGGA

Product: multidrug efflux transporter, permease protein

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 542; Mature: 541

Protein sequence:

>542_residues
MTEAAIHPDSGQAKAAAQPHAYMGAGGEAVDWMKIFLGFGGMVIGQFMAMLDIQIVASSLVQIQAGIGATADEISWVQTI
YLLAEVVIMPLTAYLTKMWGTRSFYVIAVVGFIVTSVAVGLSSSVGMMIFFRALQGVFAGAMIPPIFATAMTVFPPDKRI
TANVIVGLIVTLAPTVGPTLGGHLTEALNWRWLFFINVPVGALVIFLVGRYGDFDKGDPSLSKGIDWWGLGLMTVFLLSM
QYVLEEGNDEGWFEADLILWLTVTAALTGVAFIWRQLAYRQPIVSLTPFRDRNFTLGIIMTFISGASLFGGTFLLPIFLG
QIRGFSSAEVGTTMLVSGLSMFLSAPLAGRLVRMVDARISMVVGFALAAWAIQLGVRVTDEWGFREFFVLQAVRGFGTMI
AMIASQQMAVSTLPVSAMKDASGLINLVRNVAGAIGLALLSTILTHQAAVHYADLASAVSIANPQSTAMFDGLVQMMTAG
GMADPEGGARKAFSMMMHRQASTLSFGDAFGVLAMGCWVAAALAFFARPGRPGAAPANTEAH

Sequences:

>Translated_542_residues
MTEAAIHPDSGQAKAAAQPHAYMGAGGEAVDWMKIFLGFGGMVIGQFMAMLDIQIVASSLVQIQAGIGATADEISWVQTI
YLLAEVVIMPLTAYLTKMWGTRSFYVIAVVGFIVTSVAVGLSSSVGMMIFFRALQGVFAGAMIPPIFATAMTVFPPDKRI
TANVIVGLIVTLAPTVGPTLGGHLTEALNWRWLFFINVPVGALVIFLVGRYGDFDKGDPSLSKGIDWWGLGLMTVFLLSM
QYVLEEGNDEGWFEADLILWLTVTAALTGVAFIWRQLAYRQPIVSLTPFRDRNFTLGIIMTFISGASLFGGTFLLPIFLG
QIRGFSSAEVGTTMLVSGLSMFLSAPLAGRLVRMVDARISMVVGFALAAWAIQLGVRVTDEWGFREFFVLQAVRGFGTMI
AMIASQQMAVSTLPVSAMKDASGLINLVRNVAGAIGLALLSTILTHQAAVHYADLASAVSIANPQSTAMFDGLVQMMTAG
GMADPEGGARKAFSMMMHRQASTLSFGDAFGVLAMGCWVAAALAFFARPGRPGAAPANTEAH
>Mature_541_residues
TEAAIHPDSGQAKAAAQPHAYMGAGGEAVDWMKIFLGFGGMVIGQFMAMLDIQIVASSLVQIQAGIGATADEISWVQTIY
LLAEVVIMPLTAYLTKMWGTRSFYVIAVVGFIVTSVAVGLSSSVGMMIFFRALQGVFAGAMIPPIFATAMTVFPPDKRIT
ANVIVGLIVTLAPTVGPTLGGHLTEALNWRWLFFINVPVGALVIFLVGRYGDFDKGDPSLSKGIDWWGLGLMTVFLLSMQ
YVLEEGNDEGWFEADLILWLTVTAALTGVAFIWRQLAYRQPIVSLTPFRDRNFTLGIIMTFISGASLFGGTFLLPIFLGQ
IRGFSSAEVGTTMLVSGLSMFLSAPLAGRLVRMVDARISMVVGFALAAWAIQLGVRVTDEWGFREFFVLQAVRGFGTMIA
MIASQQMAVSTLPVSAMKDASGLINLVRNVAGAIGLALLSTILTHQAAVHYADLASAVSIANPQSTAMFDGLVQMMTAGG
MADPEGGARKAFSMMMHRQASTLSFGDAFGVLAMGCWVAAALAFFARPGRPGAAPANTEAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=489, Percent_Identity=29.6523517382413, Blast_Score=207, Evalue=1e-54,
Organism=Escherichia coli, GI1788710, Length=493, Percent_Identity=27.5862068965517, Blast_Score=187, Evalue=2e-48,
Organism=Escherichia coli, GI87081983, Length=327, Percent_Identity=28.1345565749235, Blast_Score=99, Evalue=7e-22,
Organism=Escherichia coli, GI1790195, Length=337, Percent_Identity=23.1454005934718, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1788392, Length=295, Percent_Identity=27.7966101694915, Blast_Score=75, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6322958, Length=218, Percent_Identity=25.2293577981651, Blast_Score=69, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=132, Percent_Identity=32.5757575757576, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 57722; Mature: 57590

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
5.7 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
5.5 %Met     (Mature Protein)
5.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CC
>Mature Secondary Structure 
TEAAIHPDSGQAKAAAQPHAYMGAGGEAVDWMKIFLGFGGMVIGQFMAMLDIQIVASSL
CCCCCCCCCCCCCCCCCCCHHCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHH
VQIQAGIGATADEISWVQTIYLLAEVVIMPLTAYLTKMWGTRSFYVIAVVGFIVTSVAVG
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
LSSSVGMMIFFRALQGVFAGAMIPPIFATAMTVFPPDKRITANVIVGLIVTLAPTVGPTL
HCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCC
GGHLTEALNWRWLFFINVPVGALVIFLVGRYGDFDKGDPSLSKGIDWWGLGLMTVFLLSM
CHHHHHHCCCEEEEEEECCHHHHHHHHHHCCCCCCCCCCCHHHCCCHHHHHHHHHHHHHH
QYVLEEGNDEGWFEADLILWLTVTAALTGVAFIWRQLAYRQPIVSLTPFRDRNFTLGIIM
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCHHHHHH
TFISGASLFGGTFLLPIFLGQIRGFSSAEVGTTMLVSGLSMFLSAPLAGRLVRMVDARIS
HHHCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
MVVGFALAAWAIQLGVRVTDEWGFREFFVLQAVRGFGTMIAMIASQQMAVSTLPVSAMKD
HHHHHHHHHHHHHHCCEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHH
ASGLINLVRNVAGAIGLALLSTILTHQAAVHYADLASAVSIANPQSTAMFDGLVQMMTAG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHC
GMADPEGGARKAFSMMMHRQASTLSFGDAFGVLAMGCWVAAALAFFARPGRPGAAPANTE
CCCCCCCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
AH
CC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]