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The map label for this gene is vraR [H]

Identifier: 195978994

GI number: 195978994

Start: 1967744

End: 1968343

Strand: Direct

Name: vraR [H]

Synonym: Sez_1903

Alternate gene names: 195978994

Gene position: 1967744-1968343 (Clockwise)

Preceding gene: 195978993

Following gene: 195978995

Centisome position: 97.21

GC content: 38.5

Gene sequence:

>600_bases
ATGATAAAAATAGCAATTATTGATGATCATGAATTGGTCTTGCAAGGCCTATATGATCGGTTAAAAAAGGAAACACATTT
TGAGGTTGTCGGCGCTTTTACCAGCTACCAAGATTTGTTATTTTGCTTGAAATATAAGTCTGTTGACGTTTTGATTATTG
ATCTCATGCTAAAGGATATTCATGGCTTTGACTTAATTGCACAAATCCATCAGAAATGTCAGACCGTGCCAAAAATGATT
TTAATTTCAGGATTTTATGAGCCCTTGCTGCACAAAAGAGCCTTGGATCTAGGCATAAAGGCCTTTCTTCCAAAGGAATG
CTCCTACGACGAATTAATTAGCACTGTCTACAATGTTTCAAAGGGAAATCAGGTGATTCCAGACGACTTGCTGCAAAAGC
AAGAAAGCCATCTGCTAACCGAGTCAGAGATTGAGATATTGAGACTGATCTCAGATGAATACTCGAATGAAAAAATCTCC
AAAAAGCTCTATATCAGCAGAAGGACTGTTGATACCCATGTTTCAAATATATGTGCCAAGCTCAATGTAACAAGCCGAGT
GGGGGCAGTACGAGAGGCGATTAAGCTAAAGCTGATTTGA

Upstream 100 bases:

>100_bases
ACTGTTATCGCTGAAAAGTTAGCATTGCTGGGTGGTCAATTGCTTGTTGACAAACACCCTACGACTATTGTCATGCTCAT
TCCTAGAAAGCAGGTGAATT

Downstream 100 bases:

>100_bases
TAAATCAGCCAAAAAAGGCAGACAGATATTCGTAAATTACGGATGTCTGTTTTTTGATAGTGTTTTATCATAAGCCTTGG
TTATAAATAAGAAAAGGAGA

Product: TCS response regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 199; Mature: 199

Protein sequence:

>199_residues
MIKIAIIDDHELVLQGLYDRLKKETHFEVVGAFTSYQDLLFCLKYKSVDVLIIDLMLKDIHGFDLIAQIHQKCQTVPKMI
LISGFYEPLLHKRALDLGIKAFLPKECSYDELISTVYNVSKGNQVIPDDLLQKQESHLLTESEIEILRLISDEYSNEKIS
KKLYISRRTVDTHVSNICAKLNVTSRVGAVREAIKLKLI

Sequences:

>Translated_199_residues
MIKIAIIDDHELVLQGLYDRLKKETHFEVVGAFTSYQDLLFCLKYKSVDVLIIDLMLKDIHGFDLIAQIHQKCQTVPKMI
LISGFYEPLLHKRALDLGIKAFLPKECSYDELISTVYNVSKGNQVIPDDLLQKQESHLLTESEIEILRLISDEYSNEKIS
KKLYISRRTVDTHVSNICAKLNVTSRVGAVREAIKLKLI
>Mature_199_residues
MIKIAIIDDHELVLQGLYDRLKKETHFEVVGAFTSYQDLLFCLKYKSVDVLIIDLMLKDIHGFDLIAQIHQKCQTVPKMI
LISGFYEPLLHKRALDLGIKAFLPKECSYDELISTVYNVSKGNQVIPDDLLQKQESHLLTESEIEILRLISDEYSNEKIS
KKLYISRRTVDTHVSNICAKLNVTSRVGAVREAIKLKLI

Specific function: Member of the two-component regulatory system vraS/vraR involved in the control of the cell wall peptidoglycan biosynthesis. VraR is overexpressed in strain Mu3, which leads to vancomycin resistance [H]

COG id: COG2197

COG function: function code TK; Response regulator containing a CheY-like receiver domain and an HTH DNA-binding domain

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI1790102, Length=190, Percent_Identity=32.6315789473684, Blast_Score=101, Evalue=3e-23,
Organism=Escherichia coli, GI1788222, Length=195, Percent_Identity=27.1794871794872, Blast_Score=79, Evalue=2e-16,
Organism=Escherichia coli, GI1788521, Length=199, Percent_Identity=27.1356783919598, Blast_Score=71, Evalue=6e-14,
Organism=Escherichia coli, GI1786747, Length=194, Percent_Identity=26.8041237113402, Blast_Score=70, Evalue=7e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR000792
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00196 GerE; PF00072 Response_reg [H]

EC number: NA

Molecular weight: Translated: 22817; Mature: 22817

Theoretical pI: Translated: 7.18; Mature: 7.18

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS50043 HTH_LUXR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKIAIIDDHELVLQGLYDRLKKETHFEVVGAFTSYQDLLFCLKYKSVDVLIIDLMLKDI
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
HGFDLIAQIHQKCQTVPKMILISGFYEPLLHKRALDLGIKAFLPKECSYDELISTVYNVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
KGNQVIPDDLLQKQESHLLTESEIEILRLISDEYSNEKISKKLYISRRTVDTHVSNICAK
CCCEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LNVTSRVGAVREAIKLKLI
CCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIKIAIIDDHELVLQGLYDRLKKETHFEVVGAFTSYQDLLFCLKYKSVDVLIIDLMLKDI
CEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHC
HGFDLIAQIHQKCQTVPKMILISGFYEPLLHKRALDLGIKAFLPKECSYDELISTVYNVS
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHC
KGNQVIPDDLLQKQESHLLTESEIEILRLISDEYSNEKISKKLYISRRTVDTHVSNICAK
CCCEECCHHHHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHH
LNVTSRVGAVREAIKLKLI
CCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10708580 [H]