Click here to switch to the map view.

The map label for this gene is lysS [H]

Identifier: 195978451

GI number: 195978451

Start: 1388200

End: 1389705

Strand: Direct

Name: lysS [H]

Synonym: Sez_1346

Alternate gene names: 195978451

Gene position: 1388200-1389705 (Clockwise)

Preceding gene: 195978431

Following gene: 195978453

Centisome position: 68.58

GC content: 44.82

Gene sequence:

>1506_bases
TTGGAGGAAAACATGTCAAATCAACACATCGAAGAATTAAATGATCAACAAATTGTCCGTCGTGAAAAAATGGCAGCCTT
AGCTGAGCAAGGCATTGATCCATTTGGCAAACGCTTCGACCGAACAACTAATTCTGGTGAATTAAAGGAAAAATACTCAG
ACAAAACCAAAGAGGATTTACAAGAGCTTCAAGCCACTGCGATTATTGCTGGTCGTCTGATGACCAAGCGTGGTAAAGGA
AAGGTGGGCTTTGCTCATCTTCAGGATCGTCAAGGGCAAATCCAGATCTACGTTCGCAAGGATTCAGTTGGTGATGAAAA
CTATGACATTTTCAAGAAAGCTGACCTTGGTGATTTTATCGGGGTCGAAGGCGACATTATGCGTACTGATATGGGTGAGC
TGTCTATCAAGGCGACAAAACTAACCCACCTCTCCAAATCTCTACGTCCGCTACCAGAGAAATTTCATGGCTTAACTGAT
ATCGAAACCATTTATCGCAAACGTCATCTGGATTTGATTTCAAACCGCAGCAGCTTTGAGCGGTTCGTCACACGTTCAAA
AATGATTTCTGAAATTCGTCGTTATCTTGATGGCCTTGGCTTTTTAGAAGTGGAGACACCTGTCCTTCATAATGAAGCTG
GTGGTGCTGCTGCTCGTCCCTTTATCACTCACCACAATGCCCAAGATATTGACATGGTGCTTCGTATTGCAACAGAGCTG
CACCTCAAACGTCTCATCGTCGGTGGCATGGAACGTGTCTATGAAATTGGACGTATCTTCCGCAATGAGGGAATGGATGC
CACACACAACCCTGAATTCACTTCTATTGAGGTTTATGAAGCCTATGCAGACTATCTAGATATGATGAATCTAACCGAGG
GTATCATTCAGCATGCTGCTAAAGCCGTTAGGGGAGAGGGACCAGTTGATTATCAAGGAACTGAGATTAAGATCAATGAG
CCCTTTAAGCGTATCCATATGGTTGATGCCATCAAGGAGGTAACTGGTATTGACTTCTGGCCTGAGATGACTTTTGAGGA
AGCAACAGCTCTTGCTAACGAAAAGCATGTGCCACTTGAAAAGCACTTTACCTCTGTTGGTCATATCATTAATGCCTTCT
TTGAAGCCTTTGTCGAAGACACTCTCATTCAACCAACCTTTGTTTACGGACACCCCGTAGAGGTCTCACCACTCGCTAAG
AAAAATCCTGAGGACAGCCGCTTCACAGACCGCTTTGAGCTCTTTATCATGACCAAGGAGTACGCTAATGCCTTTACCGA
GCTAAATGATCCAATTGATCAGCTGTCACGCTTTAAGGCACAGGCAGCAGCCAAGGAGCTTGGAGACGATGAAGCAACCG
GTATTGACTACGACTTCGTTGAAGCCCTTGAGTACGGTATGCCACCAACAGGCGGGCTTGGAATCGGTATTGACCGTCTT
TGTATGCTGCTAACCAATACAACAACAATTCGCGATGTGCTCTTGTTCCCAACAATGAAGCCATAA

Upstream 100 bases:

>100_bases
ATACAAGTCAAAGGTCTATCATCTGATCTTCGATGATAGAATTCCCGCGAAATATAGGTGTATTCTATAGTCTTTTGTGT
TACAATAGGAAGGAAAAGTA

Downstream 100 bases:

>100_bases
AAAGCGACTGGCCACCAGTGATTAAAAAGAGCTGTCTAAAGCTGACTATAGCCAGCGCTAGCCAATAGGCTTTAGCGGCG
GCCATGATCTTAAGCCACTT

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 501; Mature: 501

Protein sequence:

>501_residues
MEENMSNQHIEELNDQQIVRREKMAALAEQGIDPFGKRFDRTTNSGELKEKYSDKTKEDLQELQATAIIAGRLMTKRGKG
KVGFAHLQDRQGQIQIYVRKDSVGDENYDIFKKADLGDFIGVEGDIMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTD
IETIYRKRHLDLISNRSSFERFVTRSKMISEIRRYLDGLGFLEVETPVLHNEAGGAAARPFITHHNAQDIDMVLRIATEL
HLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYEAYADYLDMMNLTEGIIQHAAKAVRGEGPVDYQGTEIKINE
PFKRIHMVDAIKEVTGIDFWPEMTFEEATALANEKHVPLEKHFTSVGHIINAFFEAFVEDTLIQPTFVYGHPVEVSPLAK
KNPEDSRFTDRFELFIMTKEYANAFTELNDPIDQLSRFKAQAAAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRL
CMLLTNTTTIRDVLLFPTMKP

Sequences:

>Translated_501_residues
MEENMSNQHIEELNDQQIVRREKMAALAEQGIDPFGKRFDRTTNSGELKEKYSDKTKEDLQELQATAIIAGRLMTKRGKG
KVGFAHLQDRQGQIQIYVRKDSVGDENYDIFKKADLGDFIGVEGDIMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTD
IETIYRKRHLDLISNRSSFERFVTRSKMISEIRRYLDGLGFLEVETPVLHNEAGGAAARPFITHHNAQDIDMVLRIATEL
HLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYEAYADYLDMMNLTEGIIQHAAKAVRGEGPVDYQGTEIKINE
PFKRIHMVDAIKEVTGIDFWPEMTFEEATALANEKHVPLEKHFTSVGHIINAFFEAFVEDTLIQPTFVYGHPVEVSPLAK
KNPEDSRFTDRFELFIMTKEYANAFTELNDPIDQLSRFKAQAAAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRL
CMLLTNTTTIRDVLLFPTMKP
>Mature_501_residues
MEENMSNQHIEELNDQQIVRREKMAALAEQGIDPFGKRFDRTTNSGELKEKYSDKTKEDLQELQATAIIAGRLMTKRGKG
KVGFAHLQDRQGQIQIYVRKDSVGDENYDIFKKADLGDFIGVEGDIMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTD
IETIYRKRHLDLISNRSSFERFVTRSKMISEIRRYLDGLGFLEVETPVLHNEAGGAAARPFITHHNAQDIDMVLRIATEL
HLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYEAYADYLDMMNLTEGIIQHAAKAVRGEGPVDYQGTEIKINE
PFKRIHMVDAIKEVTGIDFWPEMTFEEATALANEKHVPLEKHFTSVGHIINAFFEAFVEDTLIQPTFVYGHPVEVSPLAK
KNPEDSRFTDRFELFIMTKEYANAFTELNDPIDQLSRFKAQAAAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRL
CMLLTNTTTIRDVLLFPTMKP

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI5031815, Length=501, Percent_Identity=42.315369261477, Blast_Score=374, Evalue=1e-104,
Organism=Homo sapiens, GI194272210, Length=507, Percent_Identity=42.2090729783037, Blast_Score=374, Evalue=1e-103,
Organism=Homo sapiens, GI40789249, Length=284, Percent_Identity=24.6478873239437, Blast_Score=86, Evalue=1e-16,
Organism=Escherichia coli, GI1790571, Length=503, Percent_Identity=50.0994035785288, Blast_Score=474, Evalue=1e-135,
Organism=Escherichia coli, GI1789256, Length=510, Percent_Identity=49.4117647058824, Blast_Score=471, Evalue=1e-134,
Organism=Escherichia coli, GI87082379, Length=327, Percent_Identity=29.9694189602446, Blast_Score=137, Evalue=2e-33,
Organism=Escherichia coli, GI1788173, Length=273, Percent_Identity=25.6410256410256, Blast_Score=84, Evalue=3e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=500, Percent_Identity=43.4, Blast_Score=387, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17535927, Length=500, Percent_Identity=43.4, Blast_Score=387, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI71994340, Length=500, Percent_Identity=43.4, Blast_Score=386, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI32566633, Length=264, Percent_Identity=26.8939393939394, Blast_Score=76, Evalue=5e-14,
Organism=Saccharomyces cerevisiae, GI6320242, Length=494, Percent_Identity=43.9271255060729, Blast_Score=385, Evalue=1e-108,
Organism=Saccharomyces cerevisiae, GI6324256, Length=479, Percent_Identity=34.446764091858, Blast_Score=233, Evalue=6e-62,
Organism=Saccharomyces cerevisiae, GI6321807, Length=445, Percent_Identity=24.2696629213483, Blast_Score=68, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24640849, Length=487, Percent_Identity=42.299794661191, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24640851, Length=487, Percent_Identity=42.299794661191, Blast_Score=375, Evalue=1e-104,
Organism=Drosophila melanogaster, GI24584738, Length=310, Percent_Identity=26.1290322580645, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 56919; Mature: 56919

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEENMSNQHIEELNDQQIVRREKMAALAEQGIDPFGKRFDRTTNSGELKEKYSDKTKEDL
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHCCHHHHHH
QELQATAIIAGRLMTKRGKGKVGFAHLQDRQGQIQIYVRKDSVGDENYDIFKKADLGDFI
HHHHHHHHHHHHHHHCCCCCCEEEHHHCCCCCEEEEEEEECCCCCCCCCHHCCCCCCCCC
GVEGDIMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETIYRKRHLDLISNRSSFE
CCCCCEEECCCCHHEEEHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCHHHHH
RFVTRSKMISEIRRYLDGLGFLEVETPVLHNEAGGAAARPFITHHNAQDIDMVLRIATEL
HHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYEAYADYLDMMNLTEGIIQHAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
KAVRGEGPVDYQGTEIKINEPFKRIHMVDAIKEVTGIDFWPEMTFEEATALANEKHVPLE
HHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHH
KHFTSVGHIINAFFEAFVEDTLIQPTFVYGHPVEVSPLAKKNPEDSRFTDRFELFIMTKE
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCHHCCEEEEEEEEHH
YANAFTELNDPIDQLSRFKAQAAAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRL
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHH
CMLLTNTTTIRDVLLFPTMKP
HHHHHCCHHHHHHHHCCCCCC
>Mature Secondary Structure
MEENMSNQHIEELNDQQIVRREKMAALAEQGIDPFGKRFDRTTNSGELKEKYSDKTKEDL
CCCCCCHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCHHHHHHCCHHHHHH
QELQATAIIAGRLMTKRGKGKVGFAHLQDRQGQIQIYVRKDSVGDENYDIFKKADLGDFI
HHHHHHHHHHHHHHHCCCCCCEEEHHHCCCCCEEEEEEEECCCCCCCCCHHCCCCCCCCC
GVEGDIMRTDMGELSIKATKLTHLSKSLRPLPEKFHGLTDIETIYRKRHLDLISNRSSFE
CCCCCEEECCCCHHEEEHHHHHHHHHHHCCCHHHHCCCHHHHHHHHHHHHHHHHCHHHHH
RFVTRSKMISEIRRYLDGLGFLEVETPVLHNEAGGAAARPFITHHNAQDIDMVLRIATEL
HHHHHHHHHHHHHHHHCCCCEEEECCCEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHH
HLKRLIVGGMERVYEIGRIFRNEGMDATHNPEFTSIEVYEAYADYLDMMNLTEGIIQHAA
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHH
KAVRGEGPVDYQGTEIKINEPFKRIHMVDAIKEVTGIDFWPEMTFEEATALANEKHVPLE
HHHCCCCCCCCCCCEEEECCHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCCCCHH
KHFTSVGHIINAFFEAFVEDTLIQPTFVYGHPVEVSPLAKKNPEDSRFTDRFELFIMTKE
HHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCCCCCCCCHHCCEEEEEEEEHH
YANAFTELNDPIDQLSRFKAQAAAKELGDDEATGIDYDFVEALEYGMPPTGGLGIGIDRL
HHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHH
CMLLTNTTTIRDVLLFPTMKP
HHHHHCCHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]