Click here to switch to the map view.

The map label for this gene is iscS [H]

Identifier: 195978169

GI number: 195978169

Start: 1082676

End: 1083803

Strand: Direct

Name: iscS [H]

Synonym: Sez_1048

Alternate gene names: 195978169

Gene position: 1082676-1083803 (Clockwise)

Preceding gene: 195978168

Following gene: 195978170

Centisome position: 53.49

GC content: 44.24

Gene sequence:

>1128_bases
ATGATTTATTTTGATAATGCTGCTACAACACCGCTCAGTCAAGCCGCTATCAAGGCGATGACTGAGGCTATGGCTGATGA
TTTTGGGAACCCATCTAGTATTCATGCTTATGGGCGGCAGGCAAATAAACAGTTAAGAGAGTGCCGAAAAGCTATTGCCA
ACTGCCTAGGAGCTGCAGAGAGCCACATTATCTTTACCTCAGGTGGTACTGAAAGTAACAATACGGCTATCAAGGGCTAT
GCCCTAGCTAATCAGGAAAAAGGCAAGCACCTAATTACAACTGCTATTGAGCATCATTCAGTCTTAAGCACAATGGCCTA
CCTGGAAGACCGATTTGGCTTTGAGGTAACCTACCTTGAGGCTAAAAACGGAATCATTGATCTAGACCAGCTCAAAGCTG
CCCTAAGAGAGGATACAATCTTAGTCAGCATGATGTTTGCCAACAACGAGACTGGAGATTTGTTGCCTATTCAGGCTGTT
GGCAGCATGCTAAAGCAGCATCAGGCTGTCTTTCATGTTGATGCTGTCCAAGCTATTGGCAAGGTTCCAATACAGCCAGC
AGATTTGGGGATTGATTTGCTCTCTGCCTCTGCCCATAAATTTCATGGACCAAAGGGGACTGGTTTTTTATACAATAAAG
GCTTAAAGCTGGACCCCCTCTTACATGGCGGCAGTCAAGAAAACAAACGAAGAGCCGGCACTGAAAACCTGATTAATATC
ATCGGCATGACCCAAGCATTAGATGAGGCTGTCTCAAGGCTGCCACAGCATTACCAGCATGCTAAAGAGCTATCACAGCT
CTTGCTTAGTGAATTGGAAGGTATTCCTTATCAGCTCAATCATAATGAGTGTCATCTTCCCTATATCATTAATATCGGCT
TTCCCAGTTACCAAAATCATGTCTTACTGACTCAGCTTGATTTAGCAGGCTTTGCTGTATCATCAGGTTCTGCTTGTACA
GCTGGTGCTGTTGAGCCAAGTCACGTTTTGGCAGCTTATTTCGGCAAGCAGTCCCATCGTCTCACAGAAGCTATTCGAAT
TAGCTTTTCAGAGCAAAATACTCAAAAAGACGTTATCGCACTAGCAACCCAGCTCAAACATATTTTAGGAGGATCATATG
GCATTTGA

Upstream 100 bases:

>100_bases
TTAGTGCCAGTGATTTAATTGCAGAGGCTATCATTCGCATTCACGAAAGAAAACCACTAAGCCCACTGTTTTCTTACAAA
CCTGAAAGCAATAACTAATC

Downstream 100 bases:

>100_bases
AAAAGAAATTGCCCTTAAAGACTGCAAATACAGCTACAGGATTAGCCCAAAGATTAAAAAATACACCCTAAGAGACACAA
CCTTTAGTCAGACCAAGATT

Product: probable cysteine desulfurase IscS

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 375; Mature: 375

Protein sequence:

>375_residues
MIYFDNAATTPLSQAAIKAMTEAMADDFGNPSSIHAYGRQANKQLRECRKAIANCLGAAESHIIFTSGGTESNNTAIKGY
ALANQEKGKHLITTAIEHHSVLSTMAYLEDRFGFEVTYLEAKNGIIDLDQLKAALREDTILVSMMFANNETGDLLPIQAV
GSMLKQHQAVFHVDAVQAIGKVPIQPADLGIDLLSASAHKFHGPKGTGFLYNKGLKLDPLLHGGSQENKRRAGTENLINI
IGMTQALDEAVSRLPQHYQHAKELSQLLLSELEGIPYQLNHNECHLPYIINIGFPSYQNHVLLTQLDLAGFAVSSGSACT
AGAVEPSHVLAAYFGKQSHRLTEAIRISFSEQNTQKDVIALATQLKHILGGSYGI

Sequences:

>Translated_375_residues
MIYFDNAATTPLSQAAIKAMTEAMADDFGNPSSIHAYGRQANKQLRECRKAIANCLGAAESHIIFTSGGTESNNTAIKGY
ALANQEKGKHLITTAIEHHSVLSTMAYLEDRFGFEVTYLEAKNGIIDLDQLKAALREDTILVSMMFANNETGDLLPIQAV
GSMLKQHQAVFHVDAVQAIGKVPIQPADLGIDLLSASAHKFHGPKGTGFLYNKGLKLDPLLHGGSQENKRRAGTENLINI
IGMTQALDEAVSRLPQHYQHAKELSQLLLSELEGIPYQLNHNECHLPYIINIGFPSYQNHVLLTQLDLAGFAVSSGSACT
AGAVEPSHVLAAYFGKQSHRLTEAIRISFSEQNTQKDVIALATQLKHILGGSYGI
>Mature_375_residues
MIYFDNAATTPLSQAAIKAMTEAMADDFGNPSSIHAYGRQANKQLRECRKAIANCLGAAESHIIFTSGGTESNNTAIKGY
ALANQEKGKHLITTAIEHHSVLSTMAYLEDRFGFEVTYLEAKNGIIDLDQLKAALREDTILVSMMFANNETGDLLPIQAV
GSMLKQHQAVFHVDAVQAIGKVPIQPADLGIDLLSASAHKFHGPKGTGFLYNKGLKLDPLLHGGSQENKRRAGTENLINI
IGMTQALDEAVSRLPQHYQHAKELSQLLLSELEGIPYQLNHNECHLPYIINIGFPSYQNHVLLTQLDLAGFAVSSGSACT
AGAVEPSHVLAAYFGKQSHRLTEAIRISFSEQNTQKDVIALATQLKHILGGSYGI

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine [H]

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily [H]

Homologues:

Organism=Homo sapiens, GI32307132, Length=365, Percent_Identity=38.9041095890411, Blast_Score=237, Evalue=2e-62,
Organism=Homo sapiens, GI156713448, Length=412, Percent_Identity=32.5242718446602, Blast_Score=184, Evalue=9e-47,
Organism=Escherichia coli, GI48994898, Length=366, Percent_Identity=41.5300546448087, Blast_Score=250, Evalue=8e-68,
Organism=Escherichia coli, GI1787970, Length=391, Percent_Identity=25.8312020460358, Blast_Score=95, Evalue=6e-21,
Organism=Escherichia coli, GI1789175, Length=389, Percent_Identity=25.706940874036, Blast_Score=94, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI25143064, Length=366, Percent_Identity=39.0710382513661, Blast_Score=243, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17533177, Length=328, Percent_Identity=31.0975609756098, Blast_Score=140, Evalue=1e-33,
Organism=Saccharomyces cerevisiae, GI6319831, Length=365, Percent_Identity=38.0821917808219, Blast_Score=219, Evalue=7e-58,
Organism=Drosophila melanogaster, GI20129463, Length=376, Percent_Identity=36.7021276595745, Blast_Score=226, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422 [H]

Pfam domain/function: PF00266 Aminotran_5 [H]

EC number: =2.8.1.7 [H]

Molecular weight: Translated: 40684; Mature: 40684

Theoretical pI: Translated: 6.70; Mature: 6.70

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIYFDNAATTPLSQAAIKAMTEAMADDFGNPSSIHAYGRQANKQLRECRKAIANCLGAAE
CEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SHIIFTSGGTESNNTAIKGYALANQEKGKHLITTAIEHHSVLSTMAYLEDRFGFEVTYLE
CEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
AKNGIIDLDQLKAALREDTILVSMMFANNETGDLLPIQAVGSMLKQHQAVFHVDAVQAIG
CCCCEEEHHHHHHHHHCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
KVPIQPADLGIDLLSASAHKFHGPKGTGFLYNKGLKLDPLLHGGSQENKRRAGTENLINI
CCCCCCHHCCHHHHHCCHHHCCCCCCCCEEEECCCEECCEECCCCCCHHHHCCHHHHHHH
IGMTQALDEAVSRLPQHYQHAKELSQLLLSELEGIPYQLNHNECHLPYIINIGFPSYQNH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCCC
VLLTQLDLAGFAVSSGSACTAGAVEPSHVLAAYFGKQSHRLTEAIRISFSEQNTQKDVIA
EEEEEECHHCEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHEECCCCCCHHHHHH
LATQLKHILGGSYGI
HHHHHHHHHCCCCCC
>Mature Secondary Structure
MIYFDNAATTPLSQAAIKAMTEAMADDFGNPSSIHAYGRQANKQLRECRKAIANCLGAAE
CEEECCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SHIIFTSGGTESNNTAIKGYALANQEKGKHLITTAIEHHSVLSTMAYLEDRFGFEVTYLE
CEEEEECCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
AKNGIIDLDQLKAALREDTILVSMMFANNETGDLLPIQAVGSMLKQHQAVFHVDAVQAIG
CCCCEEEHHHHHHHHHCCEEEEEEEECCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHC
KVPIQPADLGIDLLSASAHKFHGPKGTGFLYNKGLKLDPLLHGGSQENKRRAGTENLINI
CCCCCCHHCCHHHHHCCHHHCCCCCCCCEEEECCCEECCEECCCCCCHHHHCCHHHHHHH
IGMTQALDEAVSRLPQHYQHAKELSQLLLSELEGIPYQLNHNECHLPYIINIGFPSYQNH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCEEEEECCCCCCCC
VLLTQLDLAGFAVSSGSACTAGAVEPSHVLAAYFGKQSHRLTEAIRISFSEQNTQKDVIA
EEEEEECHHCEEECCCCCCCCCCCCHHHHHHHHHCCHHHHHHHHHHEECCCCCCHHHHHH
LATQLKHILGGSYGI
HHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]