Click here to switch to the map view.

The map label for this gene is apbE [H]

Identifier: 195978157

GI number: 195978157

Start: 1070027

End: 1070968

Strand: Direct

Name: apbE [H]

Synonym: Sez_1034

Alternate gene names: 195978157

Gene position: 1070027-1070968 (Clockwise)

Preceding gene: 195978156

Following gene: 195978162

Centisome position: 52.86

GC content: 42.04

Gene sequence:

>942_bases
ATGATAGCTACTGATCAGATCAAAATGATGGGAACAATCATTGATCTTTACATTGACTCACCAAAGGCAAAGCAGCAAGC
AGCTACTATCCGAGAGCTACTAACCCTTTACAATCACCGTTTTTCAGCTAATGACGACAGCTCTGAATTAATGGCAATTA
ACCATATGGCAGGTATTCAGCCTGTGCGTGTGCATTCAGATTTGTTTGACTTAATCTCAATCGGAAAAGAGCATAGCTTG
GCTTGTCCAAGCAACCTTAATATCGCGCTTGGTCCACTAGTACAAAGCTGGCGAATTGGTTTTTCAGACGCTAAGCTTCC
AGCTGAAGCGACTATTGGTCACAAACTAAGACTAACCAACCCTAATCATATCATCTTAAATCAAAGCCTTGGAAGCATTT
TTTTGAAAGAAAAGGGCATGTCTATCGACTTAGGTGCCCTAGCAAAGGGCTATATTGCAGATAAGCTCATGGCATTTCTT
ATCAAAGACGGTATCACTGCTGCTCTTATCAATCTAGGTGGAAATGTCCTTGTGCATGGTACCAACAAGCAGCGACATAA
TGGCTTGTTTTACGTTGGTATCCAGCAGCCCAATCAAAAAAGAGGACAGCATATCGGTGTGATTAAGGTCAACAATAACT
CTGTAGTGACCTCAGGAATCAGCGAGAGACAATTACAAATCAAAGACAAAACTTATCATCACCTTCTTGATCCTCACACT
GGCTATCCTATCAAGACCAATATGGCTAGCCTAACTATTGTCAGCCCATCCTCACTTGATGGTGAGCTATGGAGTACTCG
TCTTTTTGGCTTAGAGCATTCCTTTGTCCTAAGTCAGCTAAATAAGCTGCCTGAGGTGGAAGGCATTATCATTACCAAAG
ATGGTCAGGTTACTATCTCAGACGGGTTAAAGGGAGCCTTTCGGCTACATTATAAGCACTAA

Upstream 100 bases:

>100_bases
TGAGGGGACCTATTTCCCGCATGGTGAGATGAAAACCAAAGCCTAATACCTAAGTCCCACTTATCTAGGTGGGCTTTTCC
TATTTGAAGAGGAGGGCTTG

Downstream 100 bases:

>100_bases
AAATCAGCTAAGTGACACTACCTAGCTGATTTTTTAGATACAAGGTGTACTCCAAGCGACCTTCAATGCCAAGTCATAAT
TGCCTGTCTAACGACTCATC

Product: thiamin biosynthesis lipoprotein ApbE

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MIATDQIKMMGTIIDLYIDSPKAKQQAATIRELLTLYNHRFSANDDSSELMAINHMAGIQPVRVHSDLFDLISIGKEHSL
ACPSNLNIALGPLVQSWRIGFSDAKLPAEATIGHKLRLTNPNHIILNQSLGSIFLKEKGMSIDLGALAKGYIADKLMAFL
IKDGITAALINLGGNVLVHGTNKQRHNGLFYVGIQQPNQKRGQHIGVIKVNNNSVVTSGISERQLQIKDKTYHHLLDPHT
GYPIKTNMASLTIVSPSSLDGELWSTRLFGLEHSFVLSQLNKLPEVEGIIITKDGQVTISDGLKGAFRLHYKH

Sequences:

>Translated_313_residues
MIATDQIKMMGTIIDLYIDSPKAKQQAATIRELLTLYNHRFSANDDSSELMAINHMAGIQPVRVHSDLFDLISIGKEHSL
ACPSNLNIALGPLVQSWRIGFSDAKLPAEATIGHKLRLTNPNHIILNQSLGSIFLKEKGMSIDLGALAKGYIADKLMAFL
IKDGITAALINLGGNVLVHGTNKQRHNGLFYVGIQQPNQKRGQHIGVIKVNNNSVVTSGISERQLQIKDKTYHHLLDPHT
GYPIKTNMASLTIVSPSSLDGELWSTRLFGLEHSFVLSQLNKLPEVEGIIITKDGQVTISDGLKGAFRLHYKH
>Mature_313_residues
MIATDQIKMMGTIIDLYIDSPKAKQQAATIRELLTLYNHRFSANDDSSELMAINHMAGIQPVRVHSDLFDLISIGKEHSL
ACPSNLNIALGPLVQSWRIGFSDAKLPAEATIGHKLRLTNPNHIILNQSLGSIFLKEKGMSIDLGALAKGYIADKLMAFL
IKDGITAALINLGGNVLVHGTNKQRHNGLFYVGIQQPNQKRGQHIGVIKVNNNSVVTSGISERQLQIKDKTYHHLLDPHT
GYPIKTNMASLTIVSPSSLDGELWSTRLFGLEHSFVLSQLNKLPEVEGIIITKDGQVTISDGLKGAFRLHYKH

Specific function: Involved in the conversion of aminoimidazole ribotide (AIR), a purine intermediate, to the 4-amino-5-hydroxymethyl-2- methyl pyrimidine (HMP) moiety of thiamine [H]

COG id: COG1477

COG function: function code H; Membrane-associated lipoprotein involved in thiamine biosynthesis

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ApbE family [H]

Homologues:

Organism=Escherichia coli, GI1788543, Length=272, Percent_Identity=27.9411764705882, Blast_Score=107, Evalue=9e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003374 [H]

Pfam domain/function: PF02424 ApbE [H]

EC number: NA

Molecular weight: Translated: 34419; Mature: 34419

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIATDQIKMMGTIIDLYIDSPKAKQQAATIRELLTLYNHRFSANDDSSELMAINHMAGIQ
CCCCHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHCCCC
PVRVHSDLFDLISIGKEHSLACPSNLNIALGPLVQSWRIGFSDAKLPAEATIGHKLRLTN
EEEEHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHCCCCCCCCCCCCCCCCCEEEECC
PNHIILNQSLGSIFLKEKGMSIDLGALAKGYIADKLMAFLIKDGITAALINLGGNVLVHG
CCEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEC
TNKQRHNGLFYVGIQQPNQKRGQHIGVIKVNNNSVVTSGISERQLQIKDKTYHHLLDPHT
CCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEEECCCCCCEEEECCCHHHHHCCCCC
GYPIKTNMASLTIVSPSSLDGELWSTRLFGLEHSFVLSQLNKLPEVEGIIITKDGQVTIS
CCCEEECCEEEEEECCCCCCCHHHHHEECCCCHHHHHHHHHCCCCCCEEEEECCCCEEEC
DGLKGAFRLHYKH
CCCCEEEEEEECC
>Mature Secondary Structure
MIATDQIKMMGTIIDLYIDSPKAKQQAATIRELLTLYNHRFSANDDSSELMAINHMAGIQ
CCCCHHHHHHEEEEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEHHHCCCC
PVRVHSDLFDLISIGKEHSLACPSNLNIALGPLVQSWRIGFSDAKLPAEATIGHKLRLTN
EEEEHHHHHHHHHCCCCCCCCCCCCCCEEECCHHHHHCCCCCCCCCCCCCCCCCEEEECC
PNHIILNQSLGSIFLKEKGMSIDLGALAKGYIADKLMAFLIKDGITAALINLGGNVLVHG
CCEEEEECCCCEEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEC
TNKQRHNGLFYVGIQQPNQKRGQHIGVIKVNNNSVVTSGISERQLQIKDKTYHHLLDPHT
CCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCEEEECCCCCCEEEECCCHHHHHCCCCC
GYPIKTNMASLTIVSPSSLDGELWSTRLFGLEHSFVLSQLNKLPEVEGIIITKDGQVTIS
CCCEEECCEEEEEECCCCCCCHHHHHEECCCCHHHHHHHHHCCCCCCEEEEECCCCEEEC
DGLKGAFRLHYKH
CCCCEEEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9665876 [H]