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The map label for this gene is topA [H]

Identifier: 195978104

GI number: 195978104

Start: 1017486

End: 1019627

Strand: Direct

Name: topA [H]

Synonym: Sez_0977

Alternate gene names: 195978104

Gene position: 1017486-1019627 (Clockwise)

Preceding gene: 195978103

Following gene: 195978105

Centisome position: 50.27

GC content: 41.69

Gene sequence:

>2142_bases
GTGTGTCAATTGGTAACAAAAACAGCAACTAAGTCAAAACAAGAGACGAAAAGGTCTGCTACAAAGAAAAAATCAGCAGC
TGCTAAAAAAAATCTAGTCATTGTTGAATCACCTGCTAAGGCTAAAACCATCGAAAAATATTTGGGACGTCAGTATAAGG
TCGTTGCCTCGGTTGGTCATATTCGTGATTTGAAAAAATCATCAATGTCTATTGATTTTGACAATCATTATGAGCCACAG
TATATTAATATTCGTGGTAAGGGTCCGCTGATCAATTCGTTGAAAAAAGAAGCAAAGAATGCTAAAAAGGTTTATCTAGC
AAGTGACCCGGATCGTGAGGGAGAGGCCATTTCGTGGCATTTATCACATATTTTGGGACTTGATCCTGATGATAACAATC
GTGTCGTTTTCAATGAAATCACAAAGGATGCCGTCAAAGAAGCCTTTGAAGGCCCAAGAAAAATTGATATGGATTTGGTT
GACTCACAGCAGGCACGTCGTGTACTGGATCGTATTGTTGGTTATTCCATTTCGCCAATTCTCTGGAAAAAGGTTAAAAA
GGGGCTTTCGGCAGGGCGCGTGCAATCGGTTGCTCTAAAGCTAATCATTGACAGAGAGCATGAGATTAAGGCCTTTGTTC
CTAAAGAATATTGGTCAATTGATGGTCTCTTTAAAAAAGGAACAAAAAAATTCCAAGCAAGCTTTTACGGCCTTAATGGC
AAAAAGATGAAGCTCAATACCAATGATGATGTTAAGCTTGTTTTGTCTCAATTAAGCAGTGATGATTTTATGGTTAGTAA
GGTTGAGAAAAAAGAACGCAGGCGTCATGCTCCTTTACCTTATACAACCTCCTCATTGCAGCAGGACGCAGCCAATAAGA
TTAACTTTCGGACTCGTAAAACAATGATGGTTGCTCAGCAGCTTTATGAGGGGATTAGTCTTGGTCCTAATGGAACACAA
GGGTTAATTACTTACATGCGTACGGACTCTACTCGTATCAGCCCAGTTGCTCAAAATGATGCCGCAGGCTTTATTGTTGA
ACGCTTCGGTAGTCAGTATTCTAAGCATGGCAATCGGGTTAAAAATGCTACTGGTGCTCAAGACGCTCATGAGGCTATTC
GACCATCTAATGTGAACCATACGCCAGAGGCCATTGCTAAATTTTTAGATAAGGATCAGCTAAAGCTCTACACCTTAATT
TGGAACCGCTTTGTCGCTAGTCAGATGACGGCTGCTGTGTTTGATACTGTTAAGGTTAATCTCGAGCAAAATGGTGTCCT
TTTTGTTGCCAATGGCAGCCAGATGAAGTTTGACGGTTATATGGCTGTTTACAATGATTCTGATAAAAGCAAGATGCTTC
CTGAAATGGCAGAAGGAGAAGTGGTTAAAAAGGTTACACTAACGCCTGAGCAGCACTTTACTCAACCACCAGCTCGTTAT
TCAGAAGCAAGCCTGATTAAAACACTGGAAGAAAACGGTGTTGGAAGGCCATCAACCTACGCTCCAACACTCGATGTGAT
TCAGCGTCGTTATTATGTTAAGCTGGCTGCTAAACGCTTTGAGCCAACGGAATTAGGCGAAATAGTTAATCAATTGATTG
TGGAATTCTTCCCTGATATTGTTGATGTCAAATTCACAGCTGATATGGAAGATAAACTTGATCAGATAGAGATCGGCAAG
GAAGCCTGGCAGAATGTGATTGACCAATTCTACAAGCCATTTGTTAAAGAGTTAACCAAGGCTGAAACGGAGATCGAAAA
AATTCAAATCAAGGACGAGCCGGCTGGTTTTGACTGTGAGCTGTGCGGCCACCCGATGGTTGTTAAGCTGGGTAGATTTG
GCAAGTTTTATGCTTGTAGTAATTTTCCTGAGTGCCATCATACAAAGGCTATTACAAAGGAAATTGGAGTTTCTTGTCCA
GTTTGTCAGCAAGGCCAGGTCATCGAGCGCAAAACAAAGCGTAACCGTATTTTCTATGGCTGCGACCGCTACCCTGAGTG
TGACTTTACCTCGTGGGATTTGCCGGTAGGACGGTCTTGTCCGAAGTCAGGCGATTACCTTGTTGAGAAAAAAATTCGTG
GAGGTAAGCAGGTTGTGTGTAGCAATGAAGCCTGTGACTATAAAGAAGAGATTGTAAAGTAG

Upstream 100 bases:

>100_bases
TATTCTTAACTTTTCTTCCTATTTATAGAAAATTTTTTGTTGACAGTTCTTAAAAAATGGGATATTATGCTAGAAGCTTT
AACCATAACTGTTATAGGAA

Downstream 100 bases:

>100_bases
GTTTGAACTATTTTAGCAGAGGTCCATTATAACGGAAATATAGACGATTTTCCATTATAATGGACTTTTTGGTTTTTGTC
TGTTATAATGGAAGTCATAA

Product: DNA topoisomerase I

Products: NA

Alternate protein names: DNA topoisomerase I; Omega-protein; Relaxing enzyme; Swivelase; Untwisting enzyme [H]

Number of amino acids: Translated: 713; Mature: 713

Protein sequence:

>713_residues
MCQLVTKTATKSKQETKRSATKKKSAAAKKNLVIVESPAKAKTIEKYLGRQYKVVASVGHIRDLKKSSMSIDFDNHYEPQ
YINIRGKGPLINSLKKEAKNAKKVYLASDPDREGEAISWHLSHILGLDPDDNNRVVFNEITKDAVKEAFEGPRKIDMDLV
DSQQARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDREHEIKAFVPKEYWSIDGLFKKGTKKFQASFYGLNG
KKMKLNTNDDVKLVLSQLSSDDFMVSKVEKKERRRHAPLPYTTSSLQQDAANKINFRTRKTMMVAQQLYEGISLGPNGTQ
GLITYMRTDSTRISPVAQNDAAGFIVERFGSQYSKHGNRVKNATGAQDAHEAIRPSNVNHTPEAIAKFLDKDQLKLYTLI
WNRFVASQMTAAVFDTVKVNLEQNGVLFVANGSQMKFDGYMAVYNDSDKSKMLPEMAEGEVVKKVTLTPEQHFTQPPARY
SEASLIKTLEENGVGRPSTYAPTLDVIQRRYYVKLAAKRFEPTELGEIVNQLIVEFFPDIVDVKFTADMEDKLDQIEIGK
EAWQNVIDQFYKPFVKELTKAETEIEKIQIKDEPAGFDCELCGHPMVVKLGRFGKFYACSNFPECHHTKAITKEIGVSCP
VCQQGQVIERKTKRNRIFYGCDRYPECDFTSWDLPVGRSCPKSGDYLVEKKIRGGKQVVCSNEACDYKEEIVK

Sequences:

>Translated_713_residues
MCQLVTKTATKSKQETKRSATKKKSAAAKKNLVIVESPAKAKTIEKYLGRQYKVVASVGHIRDLKKSSMSIDFDNHYEPQ
YINIRGKGPLINSLKKEAKNAKKVYLASDPDREGEAISWHLSHILGLDPDDNNRVVFNEITKDAVKEAFEGPRKIDMDLV
DSQQARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDREHEIKAFVPKEYWSIDGLFKKGTKKFQASFYGLNG
KKMKLNTNDDVKLVLSQLSSDDFMVSKVEKKERRRHAPLPYTTSSLQQDAANKINFRTRKTMMVAQQLYEGISLGPNGTQ
GLITYMRTDSTRISPVAQNDAAGFIVERFGSQYSKHGNRVKNATGAQDAHEAIRPSNVNHTPEAIAKFLDKDQLKLYTLI
WNRFVASQMTAAVFDTVKVNLEQNGVLFVANGSQMKFDGYMAVYNDSDKSKMLPEMAEGEVVKKVTLTPEQHFTQPPARY
SEASLIKTLEENGVGRPSTYAPTLDVIQRRYYVKLAAKRFEPTELGEIVNQLIVEFFPDIVDVKFTADMEDKLDQIEIGK
EAWQNVIDQFYKPFVKELTKAETEIEKIQIKDEPAGFDCELCGHPMVVKLGRFGKFYACSNFPECHHTKAITKEIGVSCP
VCQQGQVIERKTKRNRIFYGCDRYPECDFTSWDLPVGRSCPKSGDYLVEKKIRGGKQVVCSNEACDYKEEIVK
>Mature_713_residues
MCQLVTKTATKSKQETKRSATKKKSAAAKKNLVIVESPAKAKTIEKYLGRQYKVVASVGHIRDLKKSSMSIDFDNHYEPQ
YINIRGKGPLINSLKKEAKNAKKVYLASDPDREGEAISWHLSHILGLDPDDNNRVVFNEITKDAVKEAFEGPRKIDMDLV
DSQQARRVLDRIVGYSISPILWKKVKKGLSAGRVQSVALKLIIDREHEIKAFVPKEYWSIDGLFKKGTKKFQASFYGLNG
KKMKLNTNDDVKLVLSQLSSDDFMVSKVEKKERRRHAPLPYTTSSLQQDAANKINFRTRKTMMVAQQLYEGISLGPNGTQ
GLITYMRTDSTRISPVAQNDAAGFIVERFGSQYSKHGNRVKNATGAQDAHEAIRPSNVNHTPEAIAKFLDKDQLKLYTLI
WNRFVASQMTAAVFDTVKVNLEQNGVLFVANGSQMKFDGYMAVYNDSDKSKMLPEMAEGEVVKKVTLTPEQHFTQPPARY
SEASLIKTLEENGVGRPSTYAPTLDVIQRRYYVKLAAKRFEPTELGEIVNQLIVEFFPDIVDVKFTADMEDKLDQIEIGK
EAWQNVIDQFYKPFVKELTKAETEIEKIQIKDEPAGFDCELCGHPMVVKLGRFGKFYACSNFPECHHTKAITKEIGVSCP
VCQQGQVIERKTKRNRIFYGCDRYPECDFTSWDLPVGRSCPKSGDYLVEKKIRGGKQVVCSNEACDYKEEIVK

Specific function: The reaction catalyzed by topoisomerases leads to the conversion of one topological isomer of DNA to another [H]

COG id: COG0550

COG function: function code L; Topoisomerase IA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 Toprim domain [H]

Homologues:

Organism=Homo sapiens, GI10835218, Length=619, Percent_Identity=27.3021001615509, Blast_Score=157, Evalue=3e-38,
Organism=Homo sapiens, GI4507635, Length=611, Percent_Identity=21.7675941080196, Blast_Score=96, Evalue=1e-19,
Organism=Escherichia coli, GI1787529, Length=757, Percent_Identity=38.3091149273448, Blast_Score=493, Evalue=1e-140,
Organism=Escherichia coli, GI48994931, Length=110, Percent_Identity=39.0909090909091, Blast_Score=103, Evalue=6e-23,
Organism=Escherichia coli, GI1788061, Length=555, Percent_Identity=22.8828828828829, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32563869, Length=558, Percent_Identity=24.3727598566308, Blast_Score=128, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI17555378, Length=513, Percent_Identity=24.3664717348928, Blast_Score=117, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6323263, Length=498, Percent_Identity=23.6947791164659, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24585251, Length=615, Percent_Identity=22.4390243902439, Blast_Score=135, Evalue=1e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003601
- InterPro:   IPR013497
- InterPro:   IPR013824
- InterPro:   IPR013825
- InterPro:   IPR000380
- InterPro:   IPR003602
- InterPro:   IPR013498
- InterPro:   IPR005733
- InterPro:   IPR006171 [H]

Pfam domain/function: PF01131 Topoisom_bac; PF01751 Toprim; PF01396 zf-C4_Topoisom [H]

EC number: =5.99.1.2 [H]

Molecular weight: Translated: 80648; Mature: 80648

Theoretical pI: Translated: 9.57; Mature: 9.57

Prosite motif: PS00396 TOPOISOMERASE_I_PROK

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCQLVTKTATKSKQETKRSATKKKSAAAKKNLVIVESPAKAKTIEKYLGRQYKVVASVGH
CCCEECHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCEEEHHHHHH
IRDLKKSSMSIDFDNHYEPQYINIRGKGPLINSLKKEAKNAKKVYLASDPDREGEAISWH
HHHHHHHCCEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEE
LSHILGLDPDDNNRVVFNEITKDAVKEAFEGPRKIDMDLVDSQQARRVLDRIVGYSISPI
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCEECHHHHCCHHHHHHHHHHHCCCCCHH
LWKKVKKGLSAGRVQSVALKLIIDREHEIKAFVPKEYWSIDGLFKKGTKKFQASFYGLNG
HHHHHHHCCCCCHHHHHHEEEEECCCCCCEEECCHHHHCHHHHHHHCHHHHHHHEECCCC
KKMKLNTNDDVKLVLSQLSSDDFMVSKVEKKERRRHAPLPYTTSSLQQDAANKINFRTRK
CEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHH
TMMVAQQLYEGISLGPNGTQGLITYMRTDSTRISPVAQNDAAGFIVERFGSQYSKHGNRV
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
KNATGAQDAHEAIRPSNVNHTPEAIAKFLDKDQLKLYTLIWNRFVASQMTAAVFDTVKVN
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEE
LEQNGVLFVANGSQMKFDGYMAVYNDSDKSKMLPEMAEGEVVKKVTLTPEQHFTQPPARY
EECCCEEEEECCCEEEECCEEEEEECCCHHHHCCHHHCCCEEEEEECCCHHHCCCCCCCH
SEASLIKTLEENGVGRPSTYAPTLDVIQRRYYVKLAAKRFEPTELGEIVNQLIVEFFPDI
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
VDVKFTADMEDKLDQIEIGKEAWQNVIDQFYKPFVKELTKAETEIEKIQIKDEPAGFDCE
EEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHH
LCGHPMVVKLGRFGKFYACSNFPECHHTKAITKEIGVSCPVCQQGQVIERKTKRNRIFYG
CCCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEC
CDRYPECDFTSWDLPVGRSCPKSGDYLVEKKIRGGKQVVCSNEACDYKEEIVK
CCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCEEEECCCCCCHHHHHCC
>Mature Secondary Structure
MCQLVTKTATKSKQETKRSATKKKSAAAKKNLVIVESPAKAKTIEKYLGRQYKVVASVGH
CCCEECHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCEEEHHHHHH
IRDLKKSSMSIDFDNHYEPQYINIRGKGPLINSLKKEAKNAKKVYLASDPDREGEAISWH
HHHHHHHCCEECCCCCCCCCEEEEECCCCHHHHHHHHHCCCCEEEEECCCCCCCCEEEEE
LSHILGLDPDDNNRVVFNEITKDAVKEAFEGPRKIDMDLVDSQQARRVLDRIVGYSISPI
HHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCCCEECHHHHCCHHHHHHHHHHHCCCCCHH
LWKKVKKGLSAGRVQSVALKLIIDREHEIKAFVPKEYWSIDGLFKKGTKKFQASFYGLNG
HHHHHHHCCCCCHHHHHHEEEEECCCCCCEEECCHHHHCHHHHHHHCHHHHHHHEECCCC
KKMKLNTNDDVKLVLSQLSSDDFMVSKVEKKERRRHAPLPYTTSSLQQDAANKINFRTRK
CEEEECCCCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCHHHHH
TMMVAQQLYEGISLGPNGTQGLITYMRTDSTRISPVAQNDAAGFIVERFGSQYSKHGNRV
HHHHHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
KNATGAQDAHEAIRPSNVNHTPEAIAKFLDKDQLKLYTLIWNRFVASQMTAAVFDTVKVN
CCCCCCHHHHHHCCCCCCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEE
LEQNGVLFVANGSQMKFDGYMAVYNDSDKSKMLPEMAEGEVVKKVTLTPEQHFTQPPARY
EECCCEEEEECCCEEEECCEEEEEECCCHHHHCCHHHCCCEEEEEECCCHHHCCCCCCCH
SEASLIKTLEENGVGRPSTYAPTLDVIQRRYYVKLAAKRFEPTELGEIVNQLIVEFFPDI
HHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCC
VDVKFTADMEDKLDQIEIGKEAWQNVIDQFYKPFVKELTKAETEIEKIQIKDEPAGFDCE
EEEEEECCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCHH
LCGHPMVVKLGRFGKFYACSNFPECHHTKAITKEIGVSCPVCQQGQVIERKTKRNRIFYG
CCCCCCEEEECCCCCEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCEEEC
CDRYPECDFTSWDLPVGRSCPKSGDYLVEKKIRGGKQVVCSNEACDYKEEIVK
CCCCCCCCCCCCCCCCCCCCCCCCCEEHHHHHCCCCEEEECCCCCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11337471 [H]