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The map label for this gene is cls [C]

Identifier: 195978074

GI number: 195978074

Start: 988019

End: 989686

Strand: Direct

Name: cls [C]

Synonym: Sez_0947

Alternate gene names: 195978074

Gene position: 988019-989686 (Clockwise)

Preceding gene: 195978073

Following gene: 195978075

Centisome position: 48.81

GC content: 40.41

Gene sequence:

>1668_bases
ATGCTTGCACTAATTGCTGAAACTTTTAGCAGCAGCTTAAATCATGGCCTCCAGTTTTATGATATAATAAGAAAAAATAA
AGCTGGAGGGTTGACTATCAAAAAGAATAAAAAAGTAAGATACCTGCTCCATAAAGGGAAAAAAGGATTGCTGAGGGGGA
TTTTTAGCAGAACAACCATTATTGCTGCTTTGATCGTTCTGCAAATCTTCTTTTTATGCCAGTCCTATGCTTGGATGGAG
CAGTATCGTGTTTTAATTGCTATTATTGAGCGTATTTTTTCAATTTCAATTGTCTTGTACCTGGTGAATAGCGATATGGA
CGCTATTTCAAGGGTGACATGGCTGATTCTTATTATGATAGCCCCTTTACTAGGCTCTTTATTTCTTATTTACACTAAAT
TAGACTGGGGTTATCGTGACCTAAAGCAGCGAATTAACCATTTAGTTGACTTGTCTTCTCCTTACTTGAGAGATGATGAC
GCTATTTTACAGGCGTTGAAGGGTCATACCTCAACGACCTATCACCTCGTTCAGTATTTAGAGCGTAGTCGTGGGAATTT
TCCCATTTATCGCAAGACTCAAGCAACCTACTTTCCCATTGGAGAAGCCTTTTTTGAAGGCTTAAAAACTCAGCTTTTAT
TAGCTAAGTCCTATATTTTTCTGGAATTTTTTATCATTGCAGAGGGGCAGATGTGGGGAGAAATTTTAGGGATTTTAGAA
AAGAAGGTGCAAGAGGGAGTAGAGGTTCGCGTTCTTTTTGATGGCATGACAGAGCTGTCGACCTTATCAGCGGATTATGC
TGCCAGACTTGAACGCATTGGTATTAAGGCAAAGTCTTTTTTGCCGATCTCGCCTTTTATTTCAACCTATTACAATTATC
GGGATCATCGTAAAATTGTGGTGATTGATGGAGAGGTGGCCTTTACAGGCGGTATTAACCTAGCTGATGAGTACATTAAT
GAGGTCAAACGCTTTGGGCATTGGAAGGACGCAGGTCTTATGCTAAAGGGTGAGGCTGTTGATAGCTTTTTGATCATGTT
TTTACAAATGTGGTCAATTACCGAAAAAAGGCTGATCGTGGAGCCTTATCTATCCCAGCATACAGAGGAGGTCGCCTCAG
ATGGCTATGTCATTCCTTATGCAGACTCACCACTTGATACTGACAAAATTGGCAGAAACGTCTATATTGATATCTTGAAT
CACGCTAAGGAATATGTTTATATCATGACTCCATACCTGATTTTGGATAGCGAAACAGAGCATGCTCTCAGATTTGCAGC
AGAGCGAGGGGTGGATATTCGGATTATTATGCCAGGTAAGCCTGACAAGGCTGTTCCTTATGCCCTTGCTAAAACCTACT
ATAAAGGCTTGATCACCTCAGGAGTTAAAATCTACGAATATAGTCCGGGATTTGTGCATTCTAAGGTCTTTATTTCAGAT
GATTCTAAGGCAGTCGTTGGAACCATTAATCTGGATTATCGCAGCCTCTATCATCATTTTGAATGCGCCACCTACCTTTA
TCGTTCCTCTGTTATTGCAGACATTGTTCAGGATTTCAAAGAAACCCAGGCAAGCTCTAAGCTAGTCACAGAAAGTCAGC
TAAGGCAAAGACCTTGGTATCAGAAGCTCGTTGGTCTATTGGTTAAAACGATAGCTCCCCTCTTGTAA

Upstream 100 bases:

>100_bases
ATTGAAGGGGAATCATATCTCCTTCAATCTTTTTTAGTTTTTGAGGCAATAGGTCTTATGAGGATTACAGGCTGTAAAGG
AAGTATTGAGGTCTTTTTTA

Downstream 100 bases:

>100_bases
AGAGATGATATGTTTGCTATAATAGACCTATGCGATGAGTCGATTGTGATTTTTAAATCACAGTTGTGCTGGGGAGGTCA
TTAAGGCAGGAGCCTACTTT

Product: cardiolipin synthetase Cls

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 555; Mature: 555

Protein sequence:

>555_residues
MLALIAETFSSSLNHGLQFYDIIRKNKAGGLTIKKNKKVRYLLHKGKKGLLRGIFSRTTIIAALIVLQIFFLCQSYAWME
QYRVLIAIIERIFSISIVLYLVNSDMDAISRVTWLILIMIAPLLGSLFLIYTKLDWGYRDLKQRINHLVDLSSPYLRDDD
AILQALKGHTSTTYHLVQYLERSRGNFPIYRKTQATYFPIGEAFFEGLKTQLLLAKSYIFLEFFIIAEGQMWGEILGILE
KKVQEGVEVRVLFDGMTELSTLSADYAARLERIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEVAFTGGINLADEYIN
EVKRFGHWKDAGLMLKGEAVDSFLIMFLQMWSITEKRLIVEPYLSQHTEEVASDGYVIPYADSPLDTDKIGRNVYIDILN
HAKEYVYIMTPYLILDSETEHALRFAAERGVDIRIIMPGKPDKAVPYALAKTYYKGLITSGVKIYEYSPGFVHSKVFISD
DSKAVVGTINLDYRSLYHHFECATYLYRSSVIADIVQDFKETQASSKLVTESQLRQRPWYQKLVGLLVKTIAPLL

Sequences:

>Translated_555_residues
MLALIAETFSSSLNHGLQFYDIIRKNKAGGLTIKKNKKVRYLLHKGKKGLLRGIFSRTTIIAALIVLQIFFLCQSYAWME
QYRVLIAIIERIFSISIVLYLVNSDMDAISRVTWLILIMIAPLLGSLFLIYTKLDWGYRDLKQRINHLVDLSSPYLRDDD
AILQALKGHTSTTYHLVQYLERSRGNFPIYRKTQATYFPIGEAFFEGLKTQLLLAKSYIFLEFFIIAEGQMWGEILGILE
KKVQEGVEVRVLFDGMTELSTLSADYAARLERIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEVAFTGGINLADEYIN
EVKRFGHWKDAGLMLKGEAVDSFLIMFLQMWSITEKRLIVEPYLSQHTEEVASDGYVIPYADSPLDTDKIGRNVYIDILN
HAKEYVYIMTPYLILDSETEHALRFAAERGVDIRIIMPGKPDKAVPYALAKTYYKGLITSGVKIYEYSPGFVHSKVFISD
DSKAVVGTINLDYRSLYHHFECATYLYRSSVIADIVQDFKETQASSKLVTESQLRQRPWYQKLVGLLVKTIAPLL
>Mature_555_residues
MLALIAETFSSSLNHGLQFYDIIRKNKAGGLTIKKNKKVRYLLHKGKKGLLRGIFSRTTIIAALIVLQIFFLCQSYAWME
QYRVLIAIIERIFSISIVLYLVNSDMDAISRVTWLILIMIAPLLGSLFLIYTKLDWGYRDLKQRINHLVDLSSPYLRDDD
AILQALKGHTSTTYHLVQYLERSRGNFPIYRKTQATYFPIGEAFFEGLKTQLLLAKSYIFLEFFIIAEGQMWGEILGILE
KKVQEGVEVRVLFDGMTELSTLSADYAARLERIGIKAKSFLPISPFISTYYNYRDHRKIVVIDGEVAFTGGINLADEYIN
EVKRFGHWKDAGLMLKGEAVDSFLIMFLQMWSITEKRLIVEPYLSQHTEEVASDGYVIPYADSPLDTDKIGRNVYIDILN
HAKEYVYIMTPYLILDSETEHALRFAAERGVDIRIIMPGKPDKAVPYALAKTYYKGLITSGVKIYEYSPGFVHSKVFISD
DSKAVVGTINLDYRSLYHHFECATYLYRSSVIADIVQDFKETQASSKLVTESQLRQRPWYQKLVGLLVKTIAPLL

Specific function: Catalyzes The Reversible Phosphatidyl Group Transfer From One Phosphatidylglycerol Molecule To Another To Form Cardiolipin (Cl) (Diphosphatidylglycerol) And Glycerol. Affects Resistance To The Gyrase Inhibitor Novobiocin. [C]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=454, Percent_Identity=25.5506607929515, Blast_Score=152, Evalue=4e-38,
Organism=Escherichia coli, GI1787007, Length=350, Percent_Identity=28, Blast_Score=117, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 63590; Mature: 63590

Theoretical pI: Translated: 9.07; Mature: 9.07

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALIAETFSSSLNHGLQFYDIIRKNKAGGLTIKKNKKVRYLLHKGKKGLLRGIFSRTTI
CCEEHHHHHHHHHHCCHHHHHHHHCCCCCCEEEECCCCEEHHHHCCCHHHHHHHHHHHHH
IAALIVLQIFFLCQSYAWMEQYRVLIAIIERIFSISIVLYLVNSDMDAISRVTWLILIMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
APLLGSLFLIYTKLDWGYRDLKQRINHLVDLSSPYLRDDDAILQALKGHTSTTYHLVQYL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
ERSRGNFPIYRKTQATYFPIGEAFFEGLKTQLLLAKSYIFLEFFIIAEGQMWGEILGILE
HHCCCCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHH
KKVQEGVEVRVLFDGMTELSTLSADYAARLERIGIKAKSFLPISPFISTYYNYRDHRKIV
HHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEE
VIDGEVAFTGGINLADEYINEVKRFGHWKDAGLMLKGEAVDSFLIMFLQMWSITEKRLIV
EEECCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCEEE
EPYLSQHTEEVASDGYVIPYADSPLDTDKIGRNVYIDILNHAKEYVYIMTPYLILDSETE
CHHHHHHHHHHHCCCEEEECCCCCCCHHHCCCEEEEHHHHCCCCEEEEEECEEEEECCHH
HALRFAAERGVDIRIIMPGKPDKAVPYALAKTYYKGLITSGVKIYEYSPGFVHSKVFISD
HHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEEEEC
DSKAVVGTINLDYRSLYHHFECATYLYRSSVIADIVQDFKETQASSKLVTESQLRQRPWY
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QKLVGLLVKTIAPLL
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MLALIAETFSSSLNHGLQFYDIIRKNKAGGLTIKKNKKVRYLLHKGKKGLLRGIFSRTTI
CCEEHHHHHHHHHHCCHHHHHHHHCCCCCCEEEECCCCEEHHHHCCCHHHHHHHHHHHHH
IAALIVLQIFFLCQSYAWMEQYRVLIAIIERIFSISIVLYLVNSDMDAISRVTWLILIMI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
APLLGSLFLIYTKLDWGYRDLKQRINHLVDLSSPYLRDDDAILQALKGHTSTTYHLVQYL
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHH
ERSRGNFPIYRKTQATYFPIGEAFFEGLKTQLLLAKSYIFLEFFIIAEGQMWGEILGILE
HHCCCCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHH
KKVQEGVEVRVLFDGMTELSTLSADYAARLERIGIKAKSFLPISPFISTYYNYRDHRKIV
HHHHCCCEEEEEECCCHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCCCEEE
VIDGEVAFTGGINLADEYINEVKRFGHWKDAGLMLKGEAVDSFLIMFLQMWSITEKRLIV
EEECCEEEECCCCHHHHHHHHHHHCCCCCCCCEEEECHHHHHHHHHHHHHHHHHHCCEEE
EPYLSQHTEEVASDGYVIPYADSPLDTDKIGRNVYIDILNHAKEYVYIMTPYLILDSETE
CHHHHHHHHHHHCCCEEEECCCCCCCHHHCCCEEEEHHHHCCCCEEEEEECEEEEECCHH
HALRFAAERGVDIRIIMPGKPDKAVPYALAKTYYKGLITSGVKIYEYSPGFVHSKVFISD
HHHHHHHHCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHCCEEEEEECCCCEEEEEEEEC
DSKAVVGTINLDYRSLYHHFECATYLYRSSVIADIVQDFKETQASSKLVTESQLRQRPWY
CCCEEEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHH
QKLVGLLVKTIAPLL
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12397186; 2761387 [H]