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The map label for this gene is rsmF [H]

Identifier: 195978037

GI number: 195978037

Start: 952182

End: 953498

Strand: Direct

Name: rsmF [H]

Synonym: Sez_0910

Alternate gene names: 195978037

Gene position: 952182-953498 (Clockwise)

Preceding gene: 195978036

Following gene: 195978038

Centisome position: 47.04

GC content: 44.5

Gene sequence:

>1317_bases
ATGAAAAGCATGCAATTACCAAACGATTTTATTGAAAAATACCAACAGCTGTTAGGCTCAGAAGCAAAAGCTTTTTTTGA
GAGCTTTGATCAGCCTGCTATCAGTGCCTATAGGATAAATCCCTTAAAACACCAGTCGGTCTGCCTTGATTCTCCTATTC
CTGATACACCATGGGGTTACTACGGCAGGGTATCAGGAAAGTCTGCTGACCACGTGTCAGGCGTGGTGTATTCCCAAGAG
CCTGCTGCTCAAATGGTTGCTCAGGTGGCCTCACCTGCCAAGGGAACTAGAGTGTTGGATTTGGCAGCAGCGCCAGGTGG
CAAGTCAACTCAGTTACTTGCCTATCTAGAGAATACAGGACTTTTAGTGAGTAATGAGATTTCGACCAAGCGAAGTAAGG
CTTTAGTTGAAAATATAGAGCGCTTTGGTGCTAGAAATGTTGTTGTGACAAATGAGTCAGCTGACAGGCTGGCAGCTGTT
TTTCCAGAATATTTTGACACTATTGTCTTTGATGGGCCTTGCTCTGGCGAGGGCATGTTTCGCAAGGATCCAAATGCTAT
GAGCTACTGGCACAAGGATTACCCAGCAGCATGTGCTAACCTGCAAAGGTCTATTTTATCAGAGGCGATACCTATGCTAA
AGCCAGGTGGTCAGCTGATTTATTCGACCTGCACCTGGTCGCCTGAAGAAAACGAAGAGGTTGTTCGCTGGCTACTAGAA
AGCTATGATTTCCTTGAGCTGGAGCATATCCCTAAGATCAACGGCATGTCAGAGGGAATCGGTATGCCACAGGCAGCTAG
AATGTACCCTCATCGCTTTAAAGGAGAGGGACAGTTTGTTGCCAAATTAAAGGATACTAGATCAACGATTTCCTCTTCAA
ACTACAAATCTCCAAGGTCTAACCTCACTCAAGAGCAGCTGAGGCTGTGGCAGGATTTTGAAAAAAATCATTTGAACATA
ACCTTGTCTGGCCTATTACAGGTCTTTGGTGATCGTTTGTACTTGTTACCAGATGGCTTACCCTGTCTTCGATCGCTTAA
AATAGCAAGAAATGGTCTGGAATTAGGCACTTTTAAGAAAAAACGCTTTGAGCCTTCCTATGCTCTGGGCTTGGCATTAA
GACCTGAAGAGGTCAGCTTATCTGTTGAGATTAACGAAGAGGACTTTGTCACATACGCCTCAGGAAATACCATTACCTTA
CCAGAATCTTATGCTACAGATTGGTATCAGCTGGCCATTAACGGAAATGGTCTGGGCTTTGCTAAAATAGTTGGTCGCAC
CCTTAAAAATAGCTTCCCTAAGGGCTTACGTTTTTAG

Upstream 100 bases:

>100_bases
GACTTTCAGTCTAGGCAGAAGGTGATGTTTGTTCCCAAGTCCAAGCTAGCCACCATTCAATCCCTGCTACATATAAACGA
GAACAATCAGTAAGATTAAG

Downstream 100 bases:

>100_bases
GGAATGGCATAGCATTAAATTTTTATAAATTTAAATAAAACTTCTTCCAAAGCATTGTTAGATATGTTACTATAAAGAAT
ATGCTGAAGCTTAATTCTTC

Product: tRNA and rRNA cytosine-C5-methylases

Products: NA

Alternate protein names: 16S rRNA m5C1407 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmF [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MKSMQLPNDFIEKYQQLLGSEAKAFFESFDQPAISAYRINPLKHQSVCLDSPIPDTPWGYYGRVSGKSADHVSGVVYSQE
PAAQMVAQVASPAKGTRVLDLAAAPGGKSTQLLAYLENTGLLVSNEISTKRSKALVENIERFGARNVVVTNESADRLAAV
FPEYFDTIVFDGPCSGEGMFRKDPNAMSYWHKDYPAACANLQRSILSEAIPMLKPGGQLIYSTCTWSPEENEEVVRWLLE
SYDFLELEHIPKINGMSEGIGMPQAARMYPHRFKGEGQFVAKLKDTRSTISSSNYKSPRSNLTQEQLRLWQDFEKNHLNI
TLSGLLQVFGDRLYLLPDGLPCLRSLKIARNGLELGTFKKKRFEPSYALGLALRPEEVSLSVEINEEDFVTYASGNTITL
PESYATDWYQLAINGNGLGFAKIVGRTLKNSFPKGLRF

Sequences:

>Translated_438_residues
MKSMQLPNDFIEKYQQLLGSEAKAFFESFDQPAISAYRINPLKHQSVCLDSPIPDTPWGYYGRVSGKSADHVSGVVYSQE
PAAQMVAQVASPAKGTRVLDLAAAPGGKSTQLLAYLENTGLLVSNEISTKRSKALVENIERFGARNVVVTNESADRLAAV
FPEYFDTIVFDGPCSGEGMFRKDPNAMSYWHKDYPAACANLQRSILSEAIPMLKPGGQLIYSTCTWSPEENEEVVRWLLE
SYDFLELEHIPKINGMSEGIGMPQAARMYPHRFKGEGQFVAKLKDTRSTISSSNYKSPRSNLTQEQLRLWQDFEKNHLNI
TLSGLLQVFGDRLYLLPDGLPCLRSLKIARNGLELGTFKKKRFEPSYALGLALRPEEVSLSVEINEEDFVTYASGNTITL
PESYATDWYQLAINGNGLGFAKIVGRTLKNSFPKGLRF
>Mature_438_residues
MKSMQLPNDFIEKYQQLLGSEAKAFFESFDQPAISAYRINPLKHQSVCLDSPIPDTPWGYYGRVSGKSADHVSGVVYSQE
PAAQMVAQVASPAKGTRVLDLAAAPGGKSTQLLAYLENTGLLVSNEISTKRSKALVENIERFGARNVVVTNESADRLAAV
FPEYFDTIVFDGPCSGEGMFRKDPNAMSYWHKDYPAACANLQRSILSEAIPMLKPGGQLIYSTCTWSPEENEEVVRWLLE
SYDFLELEHIPKINGMSEGIGMPQAARMYPHRFKGEGQFVAKLKDTRSTISSSNYKSPRSNLTQEQLRLWQDFEKNHLNI
TLSGLLQVFGDRLYLLPDGLPCLRSLKIARNGLELGTFKKKRFEPSYALGLALRPEEVSLSVEINEEDFVTYASGNTITL
PESYATDWYQLAINGNGLGFAKIVGRTLKNSFPKGLRF

Specific function: Specifically methylates the cytosine at position 1407 (m5C1407) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=305, Percent_Identity=28.1967213114754, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI76150623, Length=305, Percent_Identity=28.1967213114754, Blast_Score=116, Evalue=4e-26,
Organism=Homo sapiens, GI32698918, Length=195, Percent_Identity=33.3333333333333, Blast_Score=105, Evalue=7e-23,
Organism=Homo sapiens, GI39995082, Length=194, Percent_Identity=29.3814432989691, Blast_Score=90, Evalue=5e-18,
Organism=Homo sapiens, GI301336155, Length=194, Percent_Identity=29.3814432989691, Blast_Score=89, Evalue=6e-18,
Organism=Homo sapiens, GI11545785, Length=211, Percent_Identity=33.6492890995261, Blast_Score=86, Evalue=7e-17,
Organism=Homo sapiens, GI270288816, Length=254, Percent_Identity=27.9527559055118, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI270288818, Length=227, Percent_Identity=29.0748898678414, Blast_Score=73, Evalue=4e-13,
Organism=Homo sapiens, GI8922322, Length=179, Percent_Identity=30.7262569832402, Blast_Score=73, Evalue=6e-13,
Organism=Homo sapiens, GI23199998, Length=227, Percent_Identity=29.0748898678414, Blast_Score=73, Evalue=6e-13,
Organism=Escherichia coli, GI87081985, Length=387, Percent_Identity=33.0749354005168, Blast_Score=186, Evalue=2e-48,
Organism=Escherichia coli, GI2367212, Length=214, Percent_Identity=32.2429906542056, Blast_Score=94, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI17536757, Length=312, Percent_Identity=29.8076923076923, Blast_Score=127, Evalue=8e-30,
Organism=Caenorhabditis elegans, GI25143471, Length=177, Percent_Identity=32.2033898305085, Blast_Score=94, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6324268, Length=294, Percent_Identity=30.2721088435374, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6319447, Length=178, Percent_Identity=33.7078651685393, Blast_Score=98, Evalue=3e-21,
Organism=Drosophila melanogaster, GI22024126, Length=237, Percent_Identity=30.8016877637131, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI21355201, Length=188, Percent_Identity=30.3191489361702, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI21356513, Length=177, Percent_Identity=28.8135593220339, Blast_Score=82, Evalue=6e-16,
Organism=Drosophila melanogaster, GI24668781, Length=177, Percent_Identity=28.8135593220339, Blast_Score=82, Evalue=7e-16,
Organism=Drosophila melanogaster, GI19920494, Length=156, Percent_Identity=32.6923076923077, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR011023 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu [H]

EC number: =2.1.1.178 [H]

Molecular weight: Translated: 48812; Mature: 48812

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSMQLPNDFIEKYQQLLGSEAKAFFESFDQPAISAYRINPLKHQSVCLDSPIPDTPWGY
CCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEECCCCCCCEEECCCCCCCCCCC
YGRVSGKSADHVSGVVYSQEPAAQMVAQVASPAKGTRVLDLAAAPGGKSTQLLAYLENTG
CEEECCCCCCHHEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEEECCC
LLVSNEISTKRSKALVENIERFGARNVVVTNESADRLAAVFPEYFDTIVFDGPCSGEGMF
EEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCCC
RKDPNAMSYWHKDYPAACANLQRSILSEAIPMLKPGGQLIYSTCTWSPEENEEVVRWLLE
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHH
SYDFLELEHIPKINGMSEGIGMPQAARMYPHRFKGEGQFVAKLKDTRSTISSSNYKSPRS
CCCCEEHHCCCCCCCCCCCCCCCHHHHHCCHHCCCCCCEEEEHHHHHHHHCCCCCCCCHH
NLTQEQLRLWQDFEKNHLNITLSGLLQVFGDRLYLLPDGLPCLRSLKIARNGLELGTFKK
CCCHHHHHHHHHHHHCCEEEEHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCCCHH
KRFEPSYALGLALRPEEVSLSVEINEEDFVTYASGNTITLPESYATDWYQLAINGNGLGF
HHCCCCCEEEEEECCCCEEEEEEECCCCEEEEECCCEEECCCCCCCCEEEEEECCCCCCH
AKIVGRTLKNSFPKGLRF
HHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKSMQLPNDFIEKYQQLLGSEAKAFFESFDQPAISAYRINPLKHQSVCLDSPIPDTPWGY
CCCCCCCHHHHHHHHHHHCHHHHHHHHHCCCCCCEEEEECCCCCCCEEECCCCCCCCCCC
YGRVSGKSADHVSGVVYSQEPAAQMVAQVASPAKGTRVLDLAAAPGGKSTQLLAYLENTG
CEEECCCCCCHHEEEEECCCHHHHHHHHHHCCCCCCEEEEEECCCCCCCCEEEEEEECCC
LLVSNEISTKRSKALVENIERFGARNVVVTNESADRLAAVFPEYFDTIVFDGPCSGEGMF
EEEECCCHHHHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHEEECCCCCCCCCC
RKDPNAMSYWHKDYPAACANLQRSILSEAIPMLKPGGQLIYSTCTWSPEENEEVVRWLLE
CCCCCHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCHHHHHHHHH
SYDFLELEHIPKINGMSEGIGMPQAARMYPHRFKGEGQFVAKLKDTRSTISSSNYKSPRS
CCCCEEHHCCCCCCCCCCCCCCCHHHHHCCHHCCCCCCEEEEHHHHHHHHCCCCCCCCHH
NLTQEQLRLWQDFEKNHLNITLSGLLQVFGDRLYLLPDGLPCLRSLKIARNGLELGTFKK
CCCHHHHHHHHHHHHCCEEEEHHHHHHHHCCEEEECCCCCHHHHHHHHHHCCCCCCCCHH
KRFEPSYALGLALRPEEVSLSVEINEEDFVTYASGNTITLPESYATDWYQLAINGNGLGF
HHCCCCCEEEEEECCCCEEEEEEECCCCEEEEECCCEEECCCCCCCCEEEEEECCCCCCH
AKIVGRTLKNSFPKGLRF
HHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA