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The map label for this gene is truB [H]

Identifier: 195978032

GI number: 195978032

Start: 948854

End: 949735

Strand: Direct

Name: truB [H]

Synonym: Sez_0905

Alternate gene names: 195978032

Gene position: 948854-949735 (Clockwise)

Preceding gene: 195978031

Following gene: 195978033

Centisome position: 46.88

GC content: 44.67

Gene sequence:

>882_bases
ATGATAAACGGAATAATCAATTTAAAAAAAGAAGCAGGCATGACATCACATGACGCTGTTTTTAAGCTTAGAAGGCTATT
ACAAGAAAAAAAGATTGGACATGGAGGGACGCTTGATCCTGACGTGGTTGGTGTTTTGCCTATAGCAGTTGGAAAGGCAA
CGCGAGTCATTGAATACATGACAGAAGCAGGTAAAATCTACGAGGGAGAGGTTACTCTTGGCTATTCGACAACAACAGAG
GACACAAGCGGTGAAATTGTTGACCAAACAGCAGTATCCTCAAGCCTGACTGAAGCGATGGTTGATGAGGCAATGACACA
ATTTGTTGGTCAAATCACTCAGATCCCACCGATGTATTCTGCAGTTAAGGTCAATGGGCGCAAGCTTTATGATTATGCAC
GAGCTGGTGAAGCGGTCGAGCGCCCTAAAAGACAGGTAACGATTGACCAGTTTGAGCGGACAAGTCCCCTGACCTTTGCA
GGTGGTCTTTGCCGATTTAGCTTCAGGGTGAGCTGTAGTAAGGGGACCTATGTTCGGACCTTGGCGGTTGATCTAGGAAG
AGCGCTTGGCTATGCAAGCCATATGTCTTGGCTGGTTCGGACAGCTTCTTCGGGGTTAACTTTAGATCAGTCCTATACCT
TGTCAGAGATTGCTGAGCTGCTGGATAAAAAGGACATGAGCTTTTTATTGCCTATTGAGTGTGGTGTTAGTGATTTAGCC
AGAGTAGTCGTTAATGACCAGGAGCTGAGGGAGCTTTCCTTTGGTAGAAAAGTCGCTCTATCACGTGAGGAAAATCTGTT
AGCGGCTTTTTATCAGGACAGCTTAATCGCTATTTTAGAAAAGCAGGAAGAGCTTTACAAGCCAAGAAAGGTTTTTATTT
AG

Upstream 100 bases:

>100_bases
CAATCAGGCTTATTGATAGAGGCTTTGTTGCTTGTCAAAAGCCTTATGAAATGGTTATAATAGATAGGCTAATGATCAAA
ACAATAAACGAAAGTAACAC

Downstream 100 bases:

>100_bases
AGAAGATTGATATATGGATATACAACACATTAAAAGCTATCAGGAGATTGATGAATCAGCTGATACCGTTTTAGTCTTAG
GCTACTTTGATGGTCTGCAC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase [H]

Number of amino acids: Translated: 293; Mature: 293

Protein sequence:

>293_residues
MINGIINLKKEAGMTSHDAVFKLRRLLQEKKIGHGGTLDPDVVGVLPIAVGKATRVIEYMTEAGKIYEGEVTLGYSTTTE
DTSGEIVDQTAVSSSLTEAMVDEAMTQFVGQITQIPPMYSAVKVNGRKLYDYARAGEAVERPKRQVTIDQFERTSPLTFA
GGLCRFSFRVSCSKGTYVRTLAVDLGRALGYASHMSWLVRTASSGLTLDQSYTLSEIAELLDKKDMSFLLPIECGVSDLA
RVVVNDQELRELSFGRKVALSREENLLAAFYQDSLIAILEKQEELYKPRKVFI

Sequences:

>Translated_293_residues
MINGIINLKKEAGMTSHDAVFKLRRLLQEKKIGHGGTLDPDVVGVLPIAVGKATRVIEYMTEAGKIYEGEVTLGYSTTTE
DTSGEIVDQTAVSSSLTEAMVDEAMTQFVGQITQIPPMYSAVKVNGRKLYDYARAGEAVERPKRQVTIDQFERTSPLTFA
GGLCRFSFRVSCSKGTYVRTLAVDLGRALGYASHMSWLVRTASSGLTLDQSYTLSEIAELLDKKDMSFLLPIECGVSDLA
RVVVNDQELRELSFGRKVALSREENLLAAFYQDSLIAILEKQEELYKPRKVFI
>Mature_293_residues
MINGIINLKKEAGMTSHDAVFKLRRLLQEKKIGHGGTLDPDVVGVLPIAVGKATRVIEYMTEAGKIYEGEVTLGYSTTTE
DTSGEIVDQTAVSSSLTEAMVDEAMTQFVGQITQIPPMYSAVKVNGRKLYDYARAGEAVERPKRQVTIDQFERTSPLTFA
GGLCRFSFRVSCSKGTYVRTLAVDLGRALGYASHMSWLVRTASSGLTLDQSYTLSEIAELLDKKDMSFLLPIECGVSDLA
RVVVNDQELRELSFGRKVALSREENLLAAFYQDSLIAILEKQEELYKPRKVFI

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs [H]

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI21040257, Length=232, Percent_Identity=32.7586206896552, Blast_Score=112, Evalue=5e-25,
Organism=Homo sapiens, GI215599015, Length=270, Percent_Identity=30.3703703703704, Blast_Score=92, Evalue=8e-19,
Organism=Homo sapiens, GI4503337, Length=270, Percent_Identity=30.3703703703704, Blast_Score=92, Evalue=8e-19,
Organism=Escherichia coli, GI2367200, Length=254, Percent_Identity=36.2204724409449, Blast_Score=155, Evalue=2e-39,
Organism=Caenorhabditis elegans, GI17553978, Length=269, Percent_Identity=28.2527881040892, Blast_Score=92, Evalue=4e-19,
Organism=Saccharomyces cerevisiae, GI6323204, Length=267, Percent_Identity=28.8389513108614, Blast_Score=100, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6324037, Length=130, Percent_Identity=43.0769230769231, Blast_Score=99, Evalue=8e-22,
Organism=Drosophila melanogaster, GI281364189, Length=270, Percent_Identity=28.1481481481481, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281364187, Length=270, Percent_Identity=28.1481481481481, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281364185, Length=270, Percent_Identity=28.1481481481481, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI281364183, Length=270, Percent_Identity=28.1481481481481, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI62471759, Length=270, Percent_Identity=28.1481481481481, Blast_Score=92, Evalue=6e-19,
Organism=Drosophila melanogaster, GI17975520, Length=270, Percent_Identity=28.1481481481481, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR014780 [H]

Pfam domain/function: PF01509 TruB_N [H]

EC number: 4.2.1.70

Molecular weight: Translated: 32411; Mature: 32411

Theoretical pI: Translated: 5.85; Mature: 5.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MINGIINLKKEAGMTSHDAVFKLRRLLQEKKIGHGGTLDPDVVGVLPIAVGKATRVIEYM
CCCCCEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEAGKIYEGEVTLGYSTTTEDTSGEIVDQTAVSSSLTEAMVDEAMTQFVGQITQIPPMYS
HHCCCEEECEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AVKVNGRKLYDYARAGEAVERPKRQVTIDQFERTSPLTFAGGLCRFSFRVSCSKGTYVRT
EEEECCHHHHHHHHCCHHHHCCHHCEEHHHHHCCCCCEEECCEEEEEEEEEECCCCCHHH
LAVDLGRALGYASHMSWLVRTASSGLTLDQSYTLSEIAELLDKKDMSFLLPIECGVSDLA
HHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHCCCCCCEEEEHHCCHHHHH
RVVVNDQELRELSFGRKVALSREENLLAAFYQDSLIAILEKQEELYKPRKVFI
HHHHCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MINGIINLKKEAGMTSHDAVFKLRRLLQEKKIGHGGTLDPDVVGVLPIAVGKATRVIEYM
CCCCCEEEHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH
TEAGKIYEGEVTLGYSTTTEDTSGEIVDQTAVSSSLTEAMVDEAMTQFVGQITQIPPMYS
HHCCCEEECEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHH
AVKVNGRKLYDYARAGEAVERPKRQVTIDQFERTSPLTFAGGLCRFSFRVSCSKGTYVRT
EEEECCHHHHHHHHCCHHHHCCHHCEEHHHHHCCCCCEEECCEEEEEEEEEECCCCCHHH
LAVDLGRALGYASHMSWLVRTASSGLTLDQSYTLSEIAELLDKKDMSFLLPIECGVSDLA
HHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCHHHHHHHHCCCCCCEEEEHHCCHHHHH
RVVVNDQELRELSFGRKVALSREENLLAAFYQDSLIAILEKQEELYKPRKVFI
HHHHCHHHHHHHHCCCCEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]