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The map label for this gene is rpoE [H]

Identifier: 195977949

GI number: 195977949

Start: 858603

End: 859142

Strand: Direct

Name: rpoE [H]

Synonym: Sez_0821

Alternate gene names: 195977949

Gene position: 858603-859142 (Clockwise)

Preceding gene: 195977948

Following gene: 195977950

Centisome position: 42.42

GC content: 34.81

Gene sequence:

>540_bases
ATGATTAAGGGTGTATTGAGAGGAGACAAGCTGTTGGAATTTGAGGACATATATTTAGAATATAAAGATGATGTCTATAG
GTTTGTAAATAAGCTGTGCTGTTATCAAAATAGTTTATCAGATGATATTACTCAAGAGACTTTTCTAAAGGCTTATCGTA
ATATTGCTAAATTTCGTGGGGATAGCTCAATCAAAACATGGTTATTTACCATCGCAAAGAATACCTTCTTTTCGGAAATT
AAAAAGAAAAAATATGAGAATATGTCGATGGAAAGCTTAGAAATCGTTGATGCTAAAGGGCATGATGAACCACTAGATAA
GATAGCAAGAAGGGAGCTGTTAGAGATTACACTAACGATTATTTTTAATCTTCCAGAGAAAATGCAGGCTGTCTTTCTGG
CGAGGATTTATTCGGGAGATTCCTATGAAAAAATAGCTAAGGATATTGGCATCACAGTATCCTCTGCCAAGGTTCTTGTC
TTTAGGGCAAGAAAACATATTAAAAGACAATTACGAGAGGAGTATGGCTATGAGGTATGA

Upstream 100 bases:

>100_bases
TCAATAATAAGATGATAGATCATTACTATTTATCTGAGTTGATGACAGCTTATACTAAAAATTAAATTAATCTGTAACTT
TTGGCAGATTGGACGGTCTA

Downstream 100 bases:

>100_bases
TTGTGAGTTGATCCAGGATTTGTTGCCATTATATGTAGATGATACGTTGAGTGATTATAGTAGAGCGGTTGTTGAGGCTC
ATCTGACAAGCTGCAAGGAC

Product: RNA polymerase sigma-E factor RpoE

Products: NA

Alternate protein names: Sigma-24 [H]

Number of amino acids: Translated: 179; Mature: 179

Protein sequence:

>179_residues
MIKGVLRGDKLLEFEDIYLEYKDDVYRFVNKLCCYQNSLSDDITQETFLKAYRNIAKFRGDSSIKTWLFTIAKNTFFSEI
KKKKYENMSMESLEIVDAKGHDEPLDKIARRELLEITLTIIFNLPEKMQAVFLARIYSGDSYEKIAKDIGITVSSAKVLV
FRARKHIKRQLREEYGYEV

Sequences:

>Translated_179_residues
MIKGVLRGDKLLEFEDIYLEYKDDVYRFVNKLCCYQNSLSDDITQETFLKAYRNIAKFRGDSSIKTWLFTIAKNTFFSEI
KKKKYENMSMESLEIVDAKGHDEPLDKIARRELLEITLTIIFNLPEKMQAVFLARIYSGDSYEKIAKDIGITVSSAKVLV
FRARKHIKRQLREEYGYEV
>Mature_179_residues
MIKGVLRGDKLLEFEDIYLEYKDDVYRFVNKLCCYQNSLSDDITQETFLKAYRNIAKFRGDSSIKTWLFTIAKNTFFSEI
KKKKYENMSMESLEIVDAKGHDEPLDKIARRELLEITLTIIFNLPEKMQAVFLARIYSGDSYEKIAKDIGITVSSAKVLV
FRARKHIKRQLREEYGYEV

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in heat shock and oxidative stress response; it is believed to control protein processing

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788926, Length=180, Percent_Identity=27.7777777777778, Blast_Score=72, Evalue=3e-14,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR014286
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21050; Mature: 21050

Theoretical pI: Translated: 8.92; Mature: 8.92

Prosite motif: PS01063 SIGMA70_ECF ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKGVLRGDKLLEFEDIYLEYKDDVYRFVNKLCCYQNSLSDDITQETFLKAYRNIAKFRG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
DSSIKTWLFTIAKNTFFSEIKKKKYENMSMESLEIVDAKGHDEPLDKIARRELLEITLTI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCHHHHHHHHHHHHHHHHH
IFNLPEKMQAVFLARIYSGDSYEKIAKDIGITVSSAKVLVFRARKHIKRQLREEYGYEV
HHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MIKGVLRGDKLLEFEDIYLEYKDDVYRFVNKLCCYQNSLSDDITQETFLKAYRNIAKFRG
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCC
DSSIKTWLFTIAKNTFFSEIKKKKYENMSMESLEIVDAKGHDEPLDKIARRELLEITLTI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHEECCCCCCCHHHHHHHHHHHHHHHHH
IFNLPEKMQAVFLARIYSGDSYEKIAKDIGITVSSAKVLVFRARKHIKRQLREEYGYEV
HHCCCHHHHHHHHHHHHCCCCHHHHHHHHCCEECHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]