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The map label for this gene is ptsI [H]

Identifier: 195977895

GI number: 195977895

Start: 793008

End: 794741

Strand: Direct

Name: ptsI [H]

Synonym: Sez_0765

Alternate gene names: 195977895

Gene position: 793008-794741 (Clockwise)

Preceding gene: 195977894

Following gene: 195977896

Centisome position: 39.18

GC content: 44.58

Gene sequence:

>1734_bases
ATGACAGAAATGCTTAAAGGAATCGCAGCCTCTGACGGTGTTGCTGTTGCCAAAGCATATCTGCTAGTTCAGCCGGATTT
GTCATTTGAGACTATCACAGTCGAGGATACAAATGCAGAAGAAGCTCGCCTTGATGTTGCATTACAAGCTTCACAAGACG
AGCTTTCTGTTATCCGAGAAAAAGCAGTAGAAAGCTTAGGCGAAGAGGCGGCAGCAGTTTTTGATGCCCATTTGATGGTT
CTTGCTGATCCGGAGATGATCAGCCAAATTAAAGAGACTATTCGTGCGAAGCAAACGAATGCCGAAACAGGTCTAAAAGA
AGTGACTGACATGTTCATCACAATCTTTGAAGGCATGGAAGACAATCCTTATATGCAAGAGCGTGCGGCTGATATTCGAG
ATGTCGCTAAGCGCGTCTTGGCTCATTTATTAGGTGTTAAGCTTCCAAATCCAGCTACAATTGATGAAGAAGCAATTGTT
ATTGCACATGATTTGACTCCTTCAGATACTGCACAGCTTAACAAACAATTTGTAAAAGCATTTGTAACGAACATTGGCGG
ACGTACGAGTCATTCGGCTATCATGGCCCGTACACTTGAGATTGCGGCTGTGCTTGGTACAAATGATATTACTAAGCGTG
TCAAAGATGGTGACCTTATTGCTGTTAACGGTATCACTGGTGAGGTTATTATTGACCCAAGTGAGGAGCAGATTCTTGCT
TTCAAGGAAGCTGGAGAGGCTTATGCGAAGCAAAAGGCTGAGTGGGCTCTGCTTAAGGACGCACAAACTGTCACAGCAGA
TGGCAAGCACTTTGAATTAGCAGCAAACATCGGTACCCCTAAGGACGTTGAAGGTGTCAACGATAATGGTGCTGAGGCGG
TAGGTCTTTACCGTACTGAGTTTCTTTATATGGATTCTCAAGATTTCCCAACCGAAGATGAGCAATATGAAGCCTACAAG
GCTGTTCTTGAAGGAATGAACGGCAAGCCTGTTGTTGTTCGAACAATGGATATCGGTGGGGACAAGGAGCTTCCTTACTT
TGATTTACCAAAGGAAATGAACCCATTCCTTGGTTTTCGTGCCTTACGTATTTCAATCTCTGAAACTGGTGATGCCATGT
TCCGCACACAAATTCGTGCTTTGCTGCGTGCTTCTGTACATGGTCAATTGCGTATCATGTTCCCGATGGTTGCCCTTCTA
AAGGAATTTCGTGCTGCTAAAGCTGTATTTGAAGAAGAAAAGGCTAGCTTGCTGGCTGAGGGTGTTGCTGTTGCAGACGA
TATTCAGGTTGGTATCATGATTGAGATCCCAGCAGCAGCAATGCTTGCTGATCAGTTTGCCAAGGAGGTTGACTTCTTCT
CAATTGGTACAAATGACCTTATCCAGTACACAATGGCAGCAGACCGCATGAACGAGCAAGTGTCATACCTTTACCAGCCA
TATAACCCATCAATCCTTCGTTTGATTAACAATGTTATCAAAGCAGCTCATGCTGAAGGTAAGTGGGCTGGTATGTGTGG
TGAAATGGCAGGTGATCAACAGGCTGTGCCGCTTCTTGTTGGTATGGGGCTTGATGAATTTTCAATGTCAGCAACATCTG
TGCTTCGCACACGTAGCCTGATGAAAAAGCTTGATTCAGCGAAAATGGCAGAGTATGCTCATCGTGCACTCACAGAATGC
TCAACTGCTGAAGAGGTTCTTGAGCTTGCCAAGGAGTATATTACAGAAGCTTAA

Upstream 100 bases:

>100_bases
CAAGGTGCTGACGTAACAATCACAGCAGAAGGTGCTGATGCAGATGACGCTATTGCAGCAATCGAAGAAACTATGACTAA
AGAAGGATTGGCATAAGACT

Downstream 100 bases:

>100_bases
TAGAAATAACGAGGAGCTGGTTTTGTCCAGCTCTTTTTATTAGAAAGATGATTAATCAAATCTGGATGAAAGCTTGCTTT
GTAAAGTAGTTTGATGTTTC

Product: phosphoenolpyruvate-protein phosphotransferase PtsI

Products: NA

Alternate protein names: Phosphotransferase system, enzyme I [H]

Number of amino acids: Translated: 577; Mature: 576

Protein sequence:

>577_residues
MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARLDVALQASQDELSVIREKAVESLGEEAAAVFDAHLMV
LADPEMISQIKETIRAKQTNAETGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATIDEEAIV
IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDLIAVNGITGEVIIDPSEEQILA
FKEAGEAYAKQKAEWALLKDAQTVTADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYK
AVLEGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRIMFPMVALL
KEFRAAKAVFEEEKASLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMAEYAHRALTEC
STAEEVLELAKEYITEA

Sequences:

>Translated_577_residues
MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARLDVALQASQDELSVIREKAVESLGEEAAAVFDAHLMV
LADPEMISQIKETIRAKQTNAETGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATIDEEAIV
IAHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDLIAVNGITGEVIIDPSEEQILA
FKEAGEAYAKQKAEWALLKDAQTVTADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYK
AVLEGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRIMFPMVALL
KEFRAAKAVFEEEKASLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMAEYAHRALTEC
STAEEVLELAKEYITEA
>Mature_576_residues
TEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARLDVALQASQDELSVIREKAVESLGEEAAAVFDAHLMVL
ADPEMISQIKETIRAKQTNAETGLKEVTDMFITIFEGMEDNPYMQERAADIRDVAKRVLAHLLGVKLPNPATIDEEAIVI
AHDLTPSDTAQLNKQFVKAFVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDLIAVNGITGEVIIDPSEEQILAF
KEAGEAYAKQKAEWALLKDAQTVTADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTEFLYMDSQDFPTEDEQYEAYKA
VLEGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFRALRISISETGDAMFRTQIRALLRASVHGQLRIMFPMVALLK
EFRAAKAVFEEEKASLLAEGVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQPY
NPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSLMKKLDSAKMAEYAHRALTECS
TAEEVLELAKEYITEA

Specific function: General (non sugar-specific) component of the phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS). This major carbohydrate active-transport system catalyzes the phosphorylation of incoming sugar substrates concomitantly with their tr

COG id: COG1080

COG function: function code G; Phosphoenolpyruvate-protein kinase (PTS system EI component in bacteria)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the PEP-utilizing enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1788756, Length=575, Percent_Identity=47.304347826087, Blast_Score=526, Evalue=1e-150,
Organism=Escherichia coli, GI48994992, Length=507, Percent_Identity=37.4753451676529, Blast_Score=337, Evalue=1e-93,
Organism=Escherichia coli, GI1788726, Length=588, Percent_Identity=35.8843537414966, Blast_Score=328, Evalue=6e-91,
Organism=Escherichia coli, GI1789193, Length=576, Percent_Identity=32.6388888888889, Blast_Score=276, Evalue=3e-75,
Organism=Escherichia coli, GI1787994, Length=421, Percent_Identity=29.6912114014252, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI226510935, Length=176, Percent_Identity=30.6818181818182, Blast_Score=78, Evalue=2e-15,

Paralogues:

None

Copy number: 360 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2659 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008279
- InterPro:   IPR006318
- InterPro:   IPR018274
- InterPro:   IPR023151
- InterPro:   IPR000121
- InterPro:   IPR008731
- InterPro:   IPR015813 [H]

Pfam domain/function: PF05524 PEP-utilisers_N; PF00391 PEP-utilizers; PF02896 PEP-utilizers_C [H]

EC number: =2.7.3.9 [H]

Molecular weight: Translated: 63185; Mature: 63054

Theoretical pI: Translated: 4.35; Mature: 4.35

Prosite motif: PS00370 PEP_ENZYMES_PHOS_SITE ; PS00742 PEP_ENZYMES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARLDVALQASQDELSVIRE
CCHHHHHHCCCCCHHEEEEEEEECCCCCEEEEEEECCCCCHHHEEEEEECCHHHHHHHHH
KAVESLGEEAAAVFDAHLMVLADPEMISQIKETIRAKQTNAETGLKEVTDMFITIFEGME
HHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
DNPYMQERAADIRDVAKRVLAHLLGVKLPNPATIDEEAIVIAHDLTPSDTAQLNKQFVKA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
FVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDLIAVNGITGEVIIDPSEEQILA
HHHHCCCCCCHHHHHHHHHHHHHEECCCHHHHHCCCCCEEEEECCCEEEEECCCHHHEEH
FKEAGEAYAKQKAEWALLKDAQTVTADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTE
HHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEE
FLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFR
EEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEE
ALRISISETGDAMFRTQIRALLRASVHGQLRIMFPMVALLKEFRAAKAVFEEEKASLLAE
EEEEEECCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
CCEEECCCEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCC
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSL
CCCHHHHHHHHHHHHHCCCCCHHHCHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
MKKLDSAKMAEYAHRALTECSTAEEVLELAKEYITEA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TEMLKGIAASDGVAVAKAYLLVQPDLSFETITVEDTNAEEARLDVALQASQDELSVIRE
CHHHHHHCCCCCHHEEEEEEEECCCCCEEEEEEECCCCCHHHEEEEEECCHHHHHHHHH
KAVESLGEEAAAVFDAHLMVLADPEMISQIKETIRAKQTNAETGLKEVTDMFITIFEGME
HHHHHHHHHHHHHHHHEEEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCC
DNPYMQERAADIRDVAKRVLAHLLGVKLPNPATIDEEAIVIAHDLTPSDTAQLNKQFVKA
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHH
FVTNIGGRTSHSAIMARTLEIAAVLGTNDITKRVKDGDLIAVNGITGEVIIDPSEEQILA
HHHHCCCCCCHHHHHHHHHHHHHEECCCHHHHHCCCCCEEEEECCCEEEEECCCHHHEEH
FKEAGEAYAKQKAEWALLKDAQTVTADGKHFELAANIGTPKDVEGVNDNGAEAVGLYRTE
HHHHHHHHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEEE
FLYMDSQDFPTEDEQYEAYKAVLEGMNGKPVVVRTMDIGGDKELPYFDLPKEMNPFLGFR
EEEECCCCCCCCCHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCCCCCCCHHCCHHHCEE
ALRISISETGDAMFRTQIRALLRASVHGQLRIMFPMVALLKEFRAAKAVFEEEKASLLAE
EEEEEECCCCHHHHHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
GVAVADDIQVGIMIEIPAAAMLADQFAKEVDFFSIGTNDLIQYTMAADRMNEQVSYLYQP
CCEEECCCEEEEEEECCHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHHHHHHHHHHCCC
YNPSILRLINNVIKAAHAEGKWAGMCGEMAGDQQAVPLLVGMGLDEFSMSATSVLRTRSL
CCCHHHHHHHHHHHHHCCCCCHHHCHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHH
MKKLDSAKMAEYAHRALTECSTAEEVLELAKEYITEA
HHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA