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The map label for this gene is prfB [H]

Identifier: 195977853

GI number: 195977853

Start: 751054

End: 752070

Strand: Direct

Name: prfB [H]

Synonym: Sez_0721

Alternate gene names: 195977853

Gene position: 751054-752070 (Clockwise)

Preceding gene: 195977851

Following gene: 195977854

Centisome position: 37.1

GC content: 45.33

Gene sequence:

>1017_bases
TTGGAGGAGGATATAGCTCTTTTAGAAAATAAGATGACAGAGCCTGATTTTTGGAATGATAATATGGCCGCTCAAAAAAC
CTCTCAGGAATTAAACGAGCTCAAGCTCACCTATCAAACCTTTCATGATATGCAGGAGCTATCTGATGAGACAGAGCTTT
ATTTAGAAATGCTTGACGAGGACGATAGCGTCAAGGAAGAGCTAGAAGCAAGCCTGATTAAGCTCAGCCAGATGCTAGCA
AGCTATGAAATGACGCTGCTTTTGTCTGAGCCCTATGATCATAGCAATGCAATCCTAGAAATTCACCCAGGTTCAGGTGG
CACTGAAGCACAAGATTGGGCAGACATGCTTTTTCGCATGTATACTCGCTTTGGAAATGCCAAGGGCTTTAAGGTAGAAA
CTCTGGACTATCAAGCAGGAGACGAGGCAGGAATTAAGTCTGTGACCCTTTCTATTGAAGGGCCAAATGCTTATGGCTTC
TTAAAATCAGAAATGGGAGTACATCGTCTGGTGCGTATTTCTCCTTTTGACTCTGCTAAGCGTCGTCACACCTCCTTTGT
TTCAGTTGAAGTCATGCCAGAGCTTGATGATACCATCGAGGTGGAGATACGTGATGATGACATTAAGATGGACACCTTCC
GCTCAGGTGGTGCTGGTGGGCAAAATGTCAACAAGGTCTCAACTGGGGTACGCTTGACCCATATCCCAACAGGAATCGTA
GTGGCGTCAACAGTTGACCGCACCCAATATGGCAACCGTGATCGCGCTATGAAAATGCTTCAAGCCAAGCTTTATCAGTT
AGAGCAGGAGAAAAAGGCAGAAGAGGTCAATGCCCTCAAGGGAGATAAAAAGGAGATCACCTGGGGCAGTCAGATCAGGT
CCTATGTCTTTACGCCTTATACGATGGTGAAGGATCATCGTACCAATTTTGAGGTGGCTCAGGTTGATAAGGTGATGGAC
GGAGATATTGAGGGTTTTATTGACGCTTATCTCAAATGGCGCATGGGTGAGGATTAA

Upstream 100 bases:

>100_bases
AGAAGAGGTAATCGTATGGAAGTGGCAGAAATGCGCCAGCAAATAGTAGAAAACAAAGAGAAGTTGACTAGCTTCAGGAG
GTCTCTTTGACTTAGAACGC

Downstream 100 bases:

>100_bases
AGTGATCCCCACTCAGATAAACACTAACAGCTTAGAGAAGGAAGAATAAGATGGCATTAATTGAAATGAAGGGTGTTTCT
AAGAAATACCGTCGCTCGAC

Product: peptide chain release factor 2

Products: NA

Alternate protein names: RF-2 [H]

Number of amino acids: Translated: 338; Mature: 338

Protein sequence:

>338_residues
MEEDIALLENKMTEPDFWNDNMAAQKTSQELNELKLTYQTFHDMQELSDETELYLEMLDEDDSVKEELEASLIKLSQMLA
SYEMTLLLSEPYDHSNAILEIHPGSGGTEAQDWADMLFRMYTRFGNAKGFKVETLDYQAGDEAGIKSVTLSIEGPNAYGF
LKSEMGVHRLVRISPFDSAKRRHTSFVSVEVMPELDDTIEVEIRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIV
VASTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAEEVNALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFEVAQVDKVMD
GDIEGFIDAYLKWRMGED

Sequences:

>Translated_338_residues
MEEDIALLENKMTEPDFWNDNMAAQKTSQELNELKLTYQTFHDMQELSDETELYLEMLDEDDSVKEELEASLIKLSQMLA
SYEMTLLLSEPYDHSNAILEIHPGSGGTEAQDWADMLFRMYTRFGNAKGFKVETLDYQAGDEAGIKSVTLSIEGPNAYGF
LKSEMGVHRLVRISPFDSAKRRHTSFVSVEVMPELDDTIEVEIRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIV
VASTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAEEVNALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFEVAQVDKVMD
GDIEGFIDAYLKWRMGED
>Mature_338_residues
MEEDIALLENKMTEPDFWNDNMAAQKTSQELNELKLTYQTFHDMQELSDETELYLEMLDEDDSVKEELEASLIKLSQMLA
SYEMTLLLSEPYDHSNAILEIHPGSGGTEAQDWADMLFRMYTRFGNAKGFKVETLDYQAGDEAGIKSVTLSIEGPNAYGF
LKSEMGVHRLVRISPFDSAKRRHTSFVSVEVMPELDDTIEVEIRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIV
VASTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAEEVNALKGDKKEITWGSQIRSYVFTPYTMVKDHRTNFEVAQVDKVMD
GDIEGFIDAYLKWRMGED

Specific function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=280, Percent_Identity=36.0714285714286, Blast_Score=155, Evalue=4e-38,
Organism=Homo sapiens, GI34577120, Length=305, Percent_Identity=32.7868852459016, Blast_Score=155, Evalue=7e-38,
Organism=Homo sapiens, GI166795305, Length=190, Percent_Identity=35.7894736842105, Blast_Score=111, Evalue=1e-24,
Organism=Escherichia coli, GI2367172, Length=324, Percent_Identity=46.6049382716049, Blast_Score=295, Evalue=4e-81,
Organism=Escherichia coli, GI1787462, Length=314, Percent_Identity=35.031847133758, Blast_Score=184, Evalue=6e-48,
Organism=Caenorhabditis elegans, GI17542784, Length=278, Percent_Identity=31.294964028777, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6321295, Length=311, Percent_Identity=31.8327974276527, Blast_Score=142, Evalue=5e-35,
Organism=Drosophila melanogaster, GI19921226, Length=272, Percent_Identity=33.0882352941176, Blast_Score=144, Evalue=6e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR020853
- InterPro:   IPR004374 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 38483; Mature: 38483

Theoretical pI: Translated: 4.38; Mature: 4.38

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
5.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
5.0 %Met     (Mature Protein)
5.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEEDIALLENKMTEPDFWNDNMAAQKTSQELNELKLTYQTFHDMQELSDETELYLEMLDE
CCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCC
DDSVKEELEASLIKLSQMLASYEMTLLLSEPYDHSNAILEIHPGSGGTEAQDWADMLFRM
CCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
YTRFGNAKGFKVETLDYQAGDEAGIKSVTLSIEGPNAYGFLKSEMGVHRLVRISPFDSAK
HHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCEEHHHHCCCEEEEEECCCCHHH
RRHTSFVSVEVMPELDDTIEVEIRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIV
HHCCEEEEEEEECCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHCCEEEEECCCCEE
VASTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAEEVNALKGDKKEITWGSQIRSYVFTPY
EEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCH
TMVKDHRTNFEVAQVDKVMDGDIEGFIDAYLKWRMGED
HEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEEDIALLENKMTEPDFWNDNMAAQKTSQELNELKLTYQTFHDMQELSDETELYLEMLDE
CCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHCC
DDSVKEELEASLIKLSQMLASYEMTLLLSEPYDHSNAILEIHPGSGGTEAQDWADMLFRM
CCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCCCCCCEEEEECCCCCCCHHHHHHHHHHH
YTRFGNAKGFKVETLDYQAGDEAGIKSVTLSIEGPNAYGFLKSEMGVHRLVRISPFDSAK
HHHHCCCCCCEEEEECCCCCCCCCCEEEEEEEECCCCCEEHHHHCCCEEEEEECCCCHHH
RRHTSFVSVEVMPELDDTIEVEIRDDDIKMDTFRSGGAGGQNVNKVSTGVRLTHIPTGIV
HHCCEEEEEEEECCCCCEEEEEEECCCEEEECCCCCCCCCCCCHHHHCCEEEEECCCCEE
VASTVDRTQYGNRDRAMKMLQAKLYQLEQEKKAEEVNALKGDKKEITWGSQIRSYVFTPY
EEEEHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCH
TMVKDHRTNFEVAQVDKVMDGDIEGFIDAYLKWRMGED
HEECCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA