Click here to switch to the map view.

The map label for this gene is uidA [H]

Identifier: 195977820

GI number: 195977820

Start: 711529

End: 713319

Strand: Direct

Name: uidA [H]

Synonym: Sez_0686

Alternate gene names: 195977820

Gene position: 711529-713319 (Clockwise)

Preceding gene: 195977819

Following gene: 195977821

Centisome position: 35.15

GC content: 43.72

Gene sequence:

>1791_bases
ATGTTATACCCAATACTGACAAAAACAAGAAAGATTTATGATTTAGGGGGGCTCTGGCAATTTAAGCTAGGAGACCATAA
CCCCCAGGAGCTTTTAGCCTCTGATGAGCTGATGGTTGTGCCCACCTCCTTTAATGATGTGGTTGTGGATAAGGACAAGC
GCAATTACATCGGTGATTTTTGGTACGAGCGCTTTGTTGATGTGCCAGATCTTGCTGCTGATGAGGAATTGGTGCTTCGC
TTTGGCTCTGTCACGCACAATGCAACAGTCTATGTTAATGGGCAAGCCTTAGGGCATCACAAGGGAGGCTTCACCCCATT
TGAAGTGCTGGTGCCTGAGGACCTTTATCAGGATAATCAAATTAAGGTATCTGTTTGTGCCAATAATATTTTAGATTATA
CCAGCCTTCCAGTGGGCAATTATAGTGAAGAGACTCAAGAGGATGGCAGTATCAAAAAGATTGTCAAGGAGAATTTTGAC
TTCTTCAACTATGCTGGTATTCACCGTCCAGTGAAGCTTATGGTGCGGCCAAAATGCCATATCTCAGATATCACGATCAC
GAGTGACTTGTCTGAGGATCTGACATCAGCCTTAGTTAAGGTATCTGTTGAAACCTCGGTGGCTGTTGATGAGGTTAAGG
TGACGATCTTTGATGAAGAGCACAAGCTCGTTGCGCAGGCTATTGACGGCAAGGCAAGACTTGATAAGGTTCGTTTATGG
GAAGTTTTAGATGCTTATCTATACACTGCCCACGTTGAGCTCATCGTAGATGGCAATGTTGTAGATAGTTATGATGAGCC
ATTTGGTATCAGAAGTATTACAGTTGAAAAAGGACAATTTTTACTAAATGGCAAGCCTGTTTATTTCAAAGGTTTTGGTA
AGCATGAGGATACCTTTATCAACGGTCGTGGATTGAATGAAGCAGCTAATTTAATGGATATGAATCTGTTCAAGGAGCTT
GGTGCCAATTCATTTCGTACCTCACACTACCCTTACTCTGAGGAGATGATGCGTCTGGCAGATCGTATGGGAATCTTGGT
TATTGATGAGGTTCCGGCAGTAGGGCTCTTCCAAAACTTTACAGCCTCCTTGGATCTTTCGCCAAAGGACAATGGCACTT
GGTCTGTCATGAAAACTAAGGAAGCGCATGAGCAAGCAATTCGTGAGCTCGTCAAGCGTGACAAAAATCACCCTTCTGTT
GTCATGTGGGTCGTGGCCAATGAGCCTGCCAGCCATGAAGAGGGAGCACACGAATACTTTGAGCCGCTGATTGCTCTTTA
TAAGGCACTAGATCCGCAGCAGCGTCCAGTAACATTGGTTAATCTGCTATTAGCAACACCTGATCGAGATAAGGTGATGG
ATCTGGTTGATGTGATCTCCTTAAACCGTTATTATGCTTGGTATGTTGATCATGGAGACTTGATCAAAGGAGAGGTCGGG
CTGCGTCAGGAGCTATTAGAATGGCAGAGCAAATTCCCAGAAAAACCAATCCTAATGACCGAATACGGTGCAGATACGCT
TCCTGGTCTTCATTCGATGTGGGATATTCCATATACAGAGGAATTTCAGTGCCGCTTCTATGAGATGAGTCACCGCGTTT
TTGATGAGATTCCTAATCTCGTCGGAGAGCAGGTATGGAATTTTGCTGATTTTGAAACTAATCTGATGATCTTCCGCATT
CAAGGCAATCACAAGGGGCTTTTTTCTAGAAATCGCCAGCCTAAGCATGTGGTTGGTTTGTTTAAAGAACGTTGGAATAC
CATTCCCCACTACAATTATAAAAAGAAATAG

Upstream 100 bases:

>100_bases
TTGAGGGTGATAGTAATATCATGACACAAGATGACATTGATCATTTACTGGCCAAGGAATCGCATGAGATTCAACGATAA
TGTGATAAAGGAGAAATCGT

Downstream 100 bases:

>100_bases
TGTACAATAGTAGCTAGCAATAAGTAGGAAGAAGGGATTGTATGGCTAGGCCTTTAGTCGAAAAAACAGCAGAGCGCTTG
TTAAAGCTAATTTTAGAACG

Product: beta-D-glucuronidase

Products: NA

Alternate protein names: GUS; Beta-D-glucuronoside glucuronosohydrolase [H]

Number of amino acids: Translated: 596; Mature: 596

Protein sequence:

>596_residues
MLYPILTKTRKIYDLGGLWQFKLGDHNPQELLASDELMVVPTSFNDVVVDKDKRNYIGDFWYERFVDVPDLAADEELVLR
FGSVTHNATVYVNGQALGHHKGGFTPFEVLVPEDLYQDNQIKVSVCANNILDYTSLPVGNYSEETQEDGSIKKIVKENFD
FFNYAGIHRPVKLMVRPKCHISDITITSDLSEDLTSALVKVSVETSVAVDEVKVTIFDEEHKLVAQAIDGKARLDKVRLW
EVLDAYLYTAHVELIVDGNVVDSYDEPFGIRSITVEKGQFLLNGKPVYFKGFGKHEDTFINGRGLNEAANLMDMNLFKEL
GANSFRTSHYPYSEEMMRLADRMGILVIDEVPAVGLFQNFTASLDLSPKDNGTWSVMKTKEAHEQAIRELVKRDKNHPSV
VMWVVANEPASHEEGAHEYFEPLIALYKALDPQQRPVTLVNLLLATPDRDKVMDLVDVISLNRYYAWYVDHGDLIKGEVG
LRQELLEWQSKFPEKPILMTEYGADTLPGLHSMWDIPYTEEFQCRFYEMSHRVFDEIPNLVGEQVWNFADFETNLMIFRI
QGNHKGLFSRNRQPKHVVGLFKERWNTIPHYNYKKK

Sequences:

>Translated_596_residues
MLYPILTKTRKIYDLGGLWQFKLGDHNPQELLASDELMVVPTSFNDVVVDKDKRNYIGDFWYERFVDVPDLAADEELVLR
FGSVTHNATVYVNGQALGHHKGGFTPFEVLVPEDLYQDNQIKVSVCANNILDYTSLPVGNYSEETQEDGSIKKIVKENFD
FFNYAGIHRPVKLMVRPKCHISDITITSDLSEDLTSALVKVSVETSVAVDEVKVTIFDEEHKLVAQAIDGKARLDKVRLW
EVLDAYLYTAHVELIVDGNVVDSYDEPFGIRSITVEKGQFLLNGKPVYFKGFGKHEDTFINGRGLNEAANLMDMNLFKEL
GANSFRTSHYPYSEEMMRLADRMGILVIDEVPAVGLFQNFTASLDLSPKDNGTWSVMKTKEAHEQAIRELVKRDKNHPSV
VMWVVANEPASHEEGAHEYFEPLIALYKALDPQQRPVTLVNLLLATPDRDKVMDLVDVISLNRYYAWYVDHGDLIKGEVG
LRQELLEWQSKFPEKPILMTEYGADTLPGLHSMWDIPYTEEFQCRFYEMSHRVFDEIPNLVGEQVWNFADFETNLMIFRI
QGNHKGLFSRNRQPKHVVGLFKERWNTIPHYNYKKK
>Mature_596_residues
MLYPILTKTRKIYDLGGLWQFKLGDHNPQELLASDELMVVPTSFNDVVVDKDKRNYIGDFWYERFVDVPDLAADEELVLR
FGSVTHNATVYVNGQALGHHKGGFTPFEVLVPEDLYQDNQIKVSVCANNILDYTSLPVGNYSEETQEDGSIKKIVKENFD
FFNYAGIHRPVKLMVRPKCHISDITITSDLSEDLTSALVKVSVETSVAVDEVKVTIFDEEHKLVAQAIDGKARLDKVRLW
EVLDAYLYTAHVELIVDGNVVDSYDEPFGIRSITVEKGQFLLNGKPVYFKGFGKHEDTFINGRGLNEAANLMDMNLFKEL
GANSFRTSHYPYSEEMMRLADRMGILVIDEVPAVGLFQNFTASLDLSPKDNGTWSVMKTKEAHEQAIRELVKRDKNHPSV
VMWVVANEPASHEEGAHEYFEPLIALYKALDPQQRPVTLVNLLLATPDRDKVMDLVDVISLNRYYAWYVDHGDLIKGEVG
LRQELLEWQSKFPEKPILMTEYGADTLPGLHSMWDIPYTEEFQCRFYEMSHRVFDEIPNLVGEQVWNFADFETNLMIFRI
QGNHKGLFSRNRQPKHVVGLFKERWNTIPHYNYKKK

Specific function: Unknown

COG id: COG3250

COG function: function code G; Beta-galactosidase/beta-glucuronidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyl hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI268834192, Length=624, Percent_Identity=39.7435897435897, Blast_Score=397, Evalue=1e-110,
Organism=Escherichia coli, GI1787903, Length=604, Percent_Identity=43.8741721854305, Blast_Score=486, Evalue=1e-138,
Organism=Escherichia coli, GI48994920, Length=417, Percent_Identity=24.9400479616307, Blast_Score=106, Evalue=5e-24,
Organism=Escherichia coli, GI1786539, Length=430, Percent_Identity=23.7209302325581, Blast_Score=101, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI17510825, Length=610, Percent_Identity=35.2459016393443, Blast_Score=358, Evalue=3e-99,
Organism=Drosophila melanogaster, GI19922582, Length=620, Percent_Identity=38.3870967741935, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI62471735, Length=620, Percent_Identity=38.3870967741935, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24655438, Length=620, Percent_Identity=38.3870967741935, Blast_Score=402, Evalue=1e-112,
Organism=Drosophila melanogaster, GI24651745, Length=628, Percent_Identity=36.4649681528662, Blast_Score=362, Evalue=1e-100,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008979
- InterPro:   IPR006101
- InterPro:   IPR013812
- InterPro:   IPR006104
- InterPro:   IPR006102
- InterPro:   IPR006103
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF00703 Glyco_hydro_2; PF02836 Glyco_hydro_2_C; PF02837 Glyco_hydro_2_N [H]

EC number: =3.2.1.31 [H]

Molecular weight: Translated: 68405; Mature: 68405

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00719 GLYCOSYL_HYDROL_F2_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLYPILTKTRKIYDLGGLWQFKLGDHNPQELLASDELMVVPTSFNDVVVDKDKRNYIGDF
CCCCCHHCCCCEEECCCEEEEECCCCCHHHHHCCCCEEEEECCCCCEEEECCCCCCHHHH
WYERFVDVPDLAADEELVLRFGSVTHNATVYVNGQALGHHKGGFTPFEVLVPEDLYQDNQ
HHHHHCCCCCCCCCHHHHHEECCCCCCEEEEEECEEECCCCCCCCCEEEECCHHHCCCCC
IKVSVCANNILDYTSLPVGNYSEETQEDGSIKKIVKENFDFFNYAGIHRPVKLMVRPKCH
EEEEEECCCCHHHCCCCCCCCCCHHHCCCHHHHHHHHCCCCEEECCCCCCEEEEECCCCC
ISDITITSDLSEDLTSALVKVSVETSVAVDEVKVTIFDEEHKLVAQAIDGKARLDKVRLW
EEEEEEECHHHHHHHHHHHEEEECCEEEEEEEEEEEECCCHHHHHHHHCCHHHHHHHHHH
EVLDAYLYTAHVELIVDGNVVDSYDEPFGIRSITVEKGQFLLNGKPVYFKGFGKHEDTFI
HHHHHHHHEEEEEEEEECCEECCCCCCCCEEEEEEECCEEEECCCEEEEECCCCCCCEEE
NGRGLNEAANLMDMNLFKELGANSFRTSHYPYSEEMMRLADRMGILVIDEVPAVGLFQNF
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHCC
TASLDLSPKDNGTWSVMKTKEAHEQAIRELVKRDKNHPSVVMWVVANEPASHEEGAHEYF
CEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHH
EPLIALYKALDPQQRPVTLVNLLLATPDRDKVMDLVDVISLNRYYAWYVDHGDLIKGEVG
HHHHHHHHHCCCCCCCHHEEHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCEEECCHH
LRQELLEWQSKFPEKPILMTEYGADTLPGLHSMWDIPYTEEFQCRFYEMSHRVFDEIPNL
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
VGEQVWNFADFETNLMIFRIQGNHKGLFSRNRQPKHVVGLFKERWNTIPHYNYKKK
HHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure
MLYPILTKTRKIYDLGGLWQFKLGDHNPQELLASDELMVVPTSFNDVVVDKDKRNYIGDF
CCCCCHHCCCCEEECCCEEEEECCCCCHHHHHCCCCEEEEECCCCCEEEECCCCCCHHHH
WYERFVDVPDLAADEELVLRFGSVTHNATVYVNGQALGHHKGGFTPFEVLVPEDLYQDNQ
HHHHHCCCCCCCCCHHHHHEECCCCCCEEEEEECEEECCCCCCCCCEEEECCHHHCCCCC
IKVSVCANNILDYTSLPVGNYSEETQEDGSIKKIVKENFDFFNYAGIHRPVKLMVRPKCH
EEEEEECCCCHHHCCCCCCCCCCHHHCCCHHHHHHHHCCCCEEECCCCCCEEEEECCCCC
ISDITITSDLSEDLTSALVKVSVETSVAVDEVKVTIFDEEHKLVAQAIDGKARLDKVRLW
EEEEEEECHHHHHHHHHHHEEEECCEEEEEEEEEEEECCCHHHHHHHHCCHHHHHHHHHH
EVLDAYLYTAHVELIVDGNVVDSYDEPFGIRSITVEKGQFLLNGKPVYFKGFGKHEDTFI
HHHHHHHHEEEEEEEEECCEECCCCCCCCEEEEEEECCEEEECCCEEEEECCCCCCCEEE
NGRGLNEAANLMDMNLFKELGANSFRTSHYPYSEEMMRLADRMGILVIDEVPAVGLFQNF
CCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCEEEEECCCCCCHHHCC
TASLDLSPKDNGTWSVMKTKEAHEQAIRELVKRDKNHPSVVMWVVANEPASHEEGAHEYF
CEEEECCCCCCCEEEEEECHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCHHHHH
EPLIALYKALDPQQRPVTLVNLLLATPDRDKVMDLVDVISLNRYYAWYVDHGDLIKGEVG
HHHHHHHHHCCCCCCCHHEEHHHHCCCCHHHHHHHHHHHHCCCEEEEEECCCCEEECCHH
LRQELLEWQSKFPEKPILMTEYGADTLPGLHSMWDIPYTEEFQCRFYEMSHRVFDEIPNL
HHHHHHHHHHHCCCCCEEEEECCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHHHHHHHH
VGEQVWNFADFETNLMIFRIQGNHKGLFSRNRQPKHVVGLFKERWNTIPHYNYKKK
HHHHHCCCCCCCCCEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3534890; 8125312; 2103475; 9097039; 9278503 [H]