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The map label for this gene is prfC [H]

Identifier: 195977781

GI number: 195977781

Start: 665324

End: 666868

Strand: Direct

Name: prfC [H]

Synonym: Sez_0645

Alternate gene names: 195977781

Gene position: 665324-666868 (Clockwise)

Preceding gene: 195977780

Following gene: 195977782

Centisome position: 32.87

GC content: 42.91

Gene sequence:

>1545_bases
ATGTCAATTACAGAAGAAATTAAAAAGCGTCGAACCTTTGCGATTATCAGTCACCCAGATGCTGGTAAAACAACTATTAC
CGAACAGTTACTTTATTTTGGTGGTGAGATTCGTGAGGCTGGGACCGTTAAAGGAAAAAAATCTGGTCAATTTGCTAAGT
CAGATTGGATGGATATTGAAAAGCAACGTGGGATTTCAGTTACTTCATCAGTTATGCAGTTTGACTATGCTGGGAAGCGT
ATCAATATCTTAGATACACCTGGTCACGAGGATTTTTCAGAGGACACCTATCGTACCTTGATGGCAGTTGATGCTGCTGT
GATGGTTGTTGACTCGGCTAAAGGGATCGAAGCACAAACAAAAAAGCTCTTTGAGGTTGTTAAGCATCGTAACATACCTG
TTTTTACGTTTATTAATAAATTAGATCGTGACGGACGTGAGCCTTTGGAGTTGTTGGAGGAGCTAGAAGAGGTCCTTGGG
ATTGCTTCTTATCCTATGAACTGGCCAATTGGTATGGGACGGGCTTTTGAAGGTTTGTATGATTTGCACAACAAGCGCTT
GGAGCTCTATAAGGGAGAGCAGCGGTTTGCTGATTTAGAGGAGGGGGCAAGGATTTTTGCCAGCAACCCTTTTTATGAGC
AGGTGCTTGAAGATATTGAGCTCTTGGAGGAGGCAGGTAATGACTTTTCAGAGCAGGCTATTTTAGATGGTCAGCTGACT
CCGGTATTCTTTGGCTCTGCCCTAACAAATTTTGGGGTACAAACCTTTTTAGATACCTTTTTAGCCTTTGCTCCGGAGCC
TCATGGGCATAAAACAACAGAGGAGCAGATGATTGATCCTCTCAATCAGGATTTTTCAGGCTTTGTCTTTAAGATTCAGG
CTAACATGGACCCTAAGCATCGTGACCGCATTGCCTTTGTGCGTATTGTCTCTGGAGAGTTTCAAAAGGGAATGGCTGTA
AAGCTGTCTCGTACAGGTAAAAGCGTTAAGCTATCAAATGTTACTCAGTTTATGGCTGAGTCTCGTGAAAATGTCCAAAA
TGCAGTTGCCGGTGATATTATTGGGGTCTACGATACAGGGACTTATCAGGTTGGAGATACCTTGATTGTTGGTAAAAACC
AGTTTGAATTTGAGCCATTGCCGACCTTTACCCCAGAGATTTTTATGAAGGTTGCACCTAAGAATGTCATGAAGCAAAAA
TCCTTCCACAAAGGTATTGAGCAGCTTGTACAGGAGGGGGCTGTTCAGCTTTATAAGAATTATCAGACAGGTGAATACAT
GCTGGGCGCAGTTGGCCAGCTGCAGTTTGAGGTTTTCAAGCACCGTATGGAGGGTGAGTACAATGCAGAGGTCATCATGA
CACCAATGGGCAAAAAGACTGTTCGTTGGATTTCAGAGGAGGATTTGGACCAACGAATGTCATCAAGCCGAAACATTTTG
GCAAGGGATCGCTTTGATCAGCCTGTTTTTCTCTTTGAAAATGACTTTGCGCTTCGTTGGTTTGCAGATAAATATCCAGA
TGTTAAGCTAGAAGCAAAAATGTAG

Upstream 100 bases:

>100_bases
AGTATTTAGGAGTGAGATCATGTAGACATAACTGTGACGTGTGACTTGAAGCTATTAATCAGAGTCTTGCTCTGCAATGT
GAACAAATAAGGAAAAAAGA

Downstream 100 bases:

>100_bases
CAGCTAAGCGGATTGGGGGAACCTAGTCCGTTTTTGTGCTTCTGCTGCTAAGAAAAGGCAAGCTATTGGCATAAGGAGCT
TTCAGGAATCCTAATTGCTG

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 514; Mature: 513

Protein sequence:

>514_residues
MSITEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKSGQFAKSDWMDIEKQRGISVTSSVMQFDYAGKR
INILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGEQRFADLEEGARIFASNPFYEQVLEDIELLEEAGNDFSEQAILDGQLT
PVFFGSALTNFGVQTFLDTFLAFAPEPHGHKTTEEQMIDPLNQDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFQKGMAV
KLSRTGKSVKLSNVTQFMAESRENVQNAVAGDIIGVYDTGTYQVGDTLIVGKNQFEFEPLPTFTPEIFMKVAPKNVMKQK
SFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAEVIMTPMGKKTVRWISEEDLDQRMSSSRNIL
ARDRFDQPVFLFENDFALRWFADKYPDVKLEAKM

Sequences:

>Translated_514_residues
MSITEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKSGQFAKSDWMDIEKQRGISVTSSVMQFDYAGKR
INILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLG
IASYPMNWPIGMGRAFEGLYDLHNKRLELYKGEQRFADLEEGARIFASNPFYEQVLEDIELLEEAGNDFSEQAILDGQLT
PVFFGSALTNFGVQTFLDTFLAFAPEPHGHKTTEEQMIDPLNQDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFQKGMAV
KLSRTGKSVKLSNVTQFMAESRENVQNAVAGDIIGVYDTGTYQVGDTLIVGKNQFEFEPLPTFTPEIFMKVAPKNVMKQK
SFHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAEVIMTPMGKKTVRWISEEDLDQRMSSSRNIL
ARDRFDQPVFLFENDFALRWFADKYPDVKLEAKM
>Mature_513_residues
SITEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKSGQFAKSDWMDIEKQRGISVTSSVMQFDYAGKRI
NILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQTKKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLGI
ASYPMNWPIGMGRAFEGLYDLHNKRLELYKGEQRFADLEEGARIFASNPFYEQVLEDIELLEEAGNDFSEQAILDGQLTP
VFFGSALTNFGVQTFLDTFLAFAPEPHGHKTTEEQMIDPLNQDFSGFVFKIQANMDPKHRDRIAFVRIVSGEFQKGMAVK
LSRTGKSVKLSNVTQFMAESRENVQNAVAGDIIGVYDTGTYQVGDTLIVGKNQFEFEPLPTFTPEIFMKVAPKNVMKQKS
FHKGIEQLVQEGAVQLYKNYQTGEYMLGAVGQLQFEVFKHRMEGEYNAEVIMTPMGKKTVRWISEEDLDQRMSSSRNILA
RDRFDQPVFLFENDFALRWFADKYPDVKLEAKM

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=485, Percent_Identity=30.7216494845361, Blast_Score=211, Evalue=1e-54,
Organism=Homo sapiens, GI19923640, Length=515, Percent_Identity=26.6019417475728, Blast_Score=171, Evalue=2e-42,
Organism=Homo sapiens, GI25306283, Length=399, Percent_Identity=29.0726817042606, Blast_Score=157, Evalue=2e-38,
Organism=Homo sapiens, GI25306287, Length=491, Percent_Identity=25.6619144602851, Blast_Score=149, Evalue=7e-36,
Organism=Homo sapiens, GI157426893, Length=157, Percent_Identity=34.3949044585987, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI94966754, Length=137, Percent_Identity=31.3868613138686, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI4503483, Length=150, Percent_Identity=30, Blast_Score=75, Evalue=1e-13,
Organism=Escherichia coli, GI1790835, Length=517, Percent_Identity=46.615087040619, Blast_Score=483, Evalue=1e-137,
Organism=Escherichia coli, GI1789738, Length=491, Percent_Identity=28.7169042769857, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI1788922, Length=160, Percent_Identity=31.875, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI48994988, Length=184, Percent_Identity=30.9782608695652, Blast_Score=92, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17533571, Length=492, Percent_Identity=29.6747967479675, Blast_Score=210, Evalue=1e-54,
Organism=Caenorhabditis elegans, GI17556745, Length=478, Percent_Identity=25.9414225941423, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17557151, Length=137, Percent_Identity=33.5766423357664, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17506493, Length=162, Percent_Identity=30.2469135802469, Blast_Score=77, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI71988819, Length=133, Percent_Identity=29.3233082706767, Blast_Score=72, Evalue=7e-13,
Organism=Caenorhabditis elegans, GI71988811, Length=133, Percent_Identity=29.3233082706767, Blast_Score=72, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6323098, Length=492, Percent_Identity=28.2520325203252, Blast_Score=197, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6322359, Length=515, Percent_Identity=27.1844660194175, Blast_Score=166, Evalue=9e-42,
Organism=Saccharomyces cerevisiae, GI6324707, Length=158, Percent_Identity=28.4810126582279, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6320593, Length=158, Percent_Identity=28.4810126582279, Blast_Score=78, Evalue=3e-15,
Organism=Saccharomyces cerevisiae, GI6323320, Length=138, Percent_Identity=29.7101449275362, Blast_Score=75, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6324761, Length=210, Percent_Identity=27.6190476190476, Blast_Score=65, Evalue=3e-11,
Organism=Drosophila melanogaster, GI24582462, Length=484, Percent_Identity=28.099173553719, Blast_Score=202, Evalue=4e-52,
Organism=Drosophila melanogaster, GI221458488, Length=510, Percent_Identity=26.2745098039216, Blast_Score=166, Evalue=4e-41,
Organism=Drosophila melanogaster, GI78706572, Length=159, Percent_Identity=33.3333333333333, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24585709, Length=158, Percent_Identity=29.746835443038, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24585711, Length=158, Percent_Identity=29.746835443038, Blast_Score=77, Evalue=3e-14,
Organism=Drosophila melanogaster, GI24585713, Length=158, Percent_Identity=29.746835443038, Blast_Score=77, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR002127
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2 [H]

EC number: NA

Molecular weight: Translated: 58518; Mature: 58387

Theoretical pI: Translated: 4.96; Mature: 4.96

Prosite motif: PS00732 RIBOSOMAL_S16 ; PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSITEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKSGQFAKSDWMDIE
CCHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
KQRGISVTSSVMQFDYAGKRINILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQT
HHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHEEEEECCCCCHHHH
KKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLGIASYPMNWPIGMGRAFEGLY
HHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
DLHNKRLELYKGEQRFADLEEGARIFASNPFYEQVLEDIELLEEAGNDFSEQAILDGQLT
HHHHHHHHHHCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHEECCCCC
PVFFGSALTNFGVQTFLDTFLAFAPEPHGHKTTEEQMIDPLNQDFSGFVFKIQANMDPKH
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCCC
RDRIAFVRIVSGEFQKGMAVKLSRTGKSVKLSNVTQFMAESRENVQNAVAGDIIGVYDTG
CCCEEEEEEECCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
TYQVGDTLIVGKNQFEFEPLPTFTPEIFMKVAPKNVMKQKSFHKGIEQLVQEGAVQLYKN
CEEECCEEEECCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
YQTGEYMLGAVGQLQFEVFKHRMEGEYNAEVIMTPMGKKTVRWISEEDLDQRMSSSRNIL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCHHHHHHHHHHCCCEE
ARDRFDQPVFLFENDFALRWFADKYPDVKLEAKM
ECCCCCCCEEEEECCEEEEEECCCCCCEEEEECC
>Mature Secondary Structure 
SITEEIKKRRTFAIISHPDAGKTTITEQLLYFGGEIREAGTVKGKKSGQFAKSDWMDIE
CHHHHHHHCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHH
KQRGISVTSSVMQFDYAGKRINILDTPGHEDFSEDTYRTLMAVDAAVMVVDSAKGIEAQT
HHCCCHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHEEEEECCCCCHHHH
KKLFEVVKHRNIPVFTFINKLDRDGREPLELLEELEEVLGIASYPMNWPIGMGRAFEGLY
HHHHHHHHHCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
DLHNKRLELYKGEQRFADLEEGARIFASNPFYEQVLEDIELLEEAGNDFSEQAILDGQLT
HHHHHHHHHHCCHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHHCCCHHHHHEECCCCC
PVFFGSALTNFGVQTFLDTFLAFAPEPHGHKTTEEQMIDPLNQDFSGFVFKIQANMDPKH
HHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCHHHHHCCCCCCCCCEEEEEECCCCCCC
RDRIAFVRIVSGEFQKGMAVKLSRTGKSVKLSNVTQFMAESRENVQNAVAGDIIGVYDTG
CCCEEEEEEECCCCCCCCEEEEECCCCCEEHHHHHHHHHHHHHHHHHHHHCCEEEEEECC
TYQVGDTLIVGKNQFEFEPLPTFTPEIFMKVAPKNVMKQKSFHKGIEQLVQEGAVQLYKN
CEEECCEEEECCCCCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
YQTGEYMLGAVGQLQFEVFKHRMEGEYNAEVIMTPMGKKTVRWISEEDLDQRMSSSRNIL
CCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHHHHCCHHHHHHHHHHCCCEE
ARDRFDQPVFLFENDFALRWFADKYPDVKLEAKM
ECCCCCCCEEEEECCEEEEEECCCCCCEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA