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The map label for this gene is metG [H]

Identifier: 195977657

GI number: 195977657

Start: 538365

End: 540383

Strand: Direct

Name: metG [H]

Synonym: Sez_0516

Alternate gene names: 195977657

Gene position: 538365-540383 (Clockwise)

Preceding gene: 195977656

Following gene: 195977658

Centisome position: 26.6

GC content: 44.82

Gene sequence:

>2019_bases
TTGAAAGGACGTAAGATGACTAAGAAAAAACCATTTTATATTACAACTCCTATTTACTACCCGTCAGGTAAGCTTCATAT
TGGCTCCGCCTATACTACGATTGCCTGTGATGTTCTTGCTCGCTATAAGCGTATGATGGGTTATGACGTTTTTTATTTGA
CTGGTCTTGATGAGCATGGTCAAAAGATTCAGGATAAGGCACAAGAAGCCGGCTTATCCCCTCAAGCTTATGTTGATGGC
ATGGCTGAGGAGGTCAAGGCACTATGGAAGCTCCTTGATATTTCTTATGATAAGTTTATCCGCACCACTGACGACTATCA
TGAGGAGGTGGTTGCTGCTGTATTTGAGAGGTTGCTGGCGCAGGATGATATTTATTTAGGGACCTATTCAGGCTGGTATT
CTGTTTCAGATGAGGAATTTTTTACAGAAAGTCAGTTAGCTGAGATTTATCGCGATGAAGCTGGCAAGGTCATTGGTGGT
GTGGCACCATCAGGGCATGAGGTCGAATGGGTCTCTGAAGAATCTTATTTTTTACGCCTGAGCAAGTATGCGGATAGATT
GGTTGCCTTCTTTAAGGCTCAGCCTGATTTTATTCAGCCGGACGGTCGCATGAATGAAATGCTTAAAAACTTCATTGAGC
CAGGTCTGGAGGATCTAGCGGTGAGTCGAACAACCTTTACCTGGGGAGTTCCTGTACCATCAGATCCTAAGCACGTTGTT
TATGTCTGGATCGATGCTCTCTTAAACTATGCAACAGCACTAGGCTACGGTCAGAAGGATCATGCTAACTTCGATAAGTT
CTGGAGTGGTAGGGTTTTCCATATGGTTGGTAAGGATATTTTGCGTTTCCACAGCATTTATTGGCCAATTTTGTTGATGA
TGCTTGATTTACCAATGCCAGAGCGTTTGATTGCTCATGGCTGGTTTGTCATGAAGGATGGTAAAATGTCTAAATCAAAG
GGAAATGTGATTTACCCTGAAATGCTGGTTGAGCGTTTTGGTCTTGATCCTCTGCGTTATTACCTCATGCGTTCCTTGCC
GGTTGGCTCTGATGGCACCTTTACCCCAGAGGATTATGTTGGTCGGATTAATTATGAGCTGGCTAATGACCTTGGTAACC
TTTTGAACCGCACAGTTGCCATGATTAATAAATACTTTGACGGCAGGGTTCCTGCTTATGTCAAAAACGTTACAGCCTTT
GATGCGGATCTGGAGCAGGTTGTGGCTGATCATATTTCCGAATACCACAAGCAAATGGAGGCTGTTGATTACCCACGTGC
CTTAGAGGCCGTATGGACGATTATTTCACGCACGAACAAATACATTGACGAGACAGCTCCTTGGGTGCTTGCCAAAGCAG
CTGATAGTAAGGAGCAGCTAGCCAGTGTGATGGCACACTTAGCTGCAAGTCTGCGCGTGGTGGCTCATTTGATTTGGCCA
TTTATGATGACGACCTCAGCTGCTATGATGGAGCAGCTAGGTCTAGATGCCACGTATCATCTGGAAGAGCTTGATTTAGC
AGATCTTCCGACAGGTGTGACGGTTGTTGCCAAAGGAGAGCCGATCTTTCCTCGTCTTGATATGGAAGCAGAGATTGACT
ATATTAAAGAGCAAATGAAAATGAGTACAGCTAAGCCGCAAGAAGCAGACTGGGATCCTGAAAAGGTAGCCCTTAAGTCT
GAAAAGGACACGATTACTTTTGAGGCCTTTGATGCAGTTGAGATTCGTGTGGCAGAGGTAAAAGAGGTGTCACGAGTTGA
AGGCTCTGAAAAATTGCTACGCTTTAGATTAGATGCAGGTGATGGCTCTGATCGTCAAATCTTATCAGGTATTGCAGCCT
ATTACCCAAATGAACAAGAGCTCGTCGGCAAGAAGGTGCAAATTGTTGCCAACCTCAAGCCGCGAAAAATGATGAAAAAA
TATATCAGTCAGGGCATGCTTTTGTCAGCAGAGCATGATGGCAAGCTGACAGTGCTGACGGTTGACCCGAGTGTGCCAAA
TGGTGCGATTATTGGCTGA

Upstream 100 bases:

>100_bases
TTTATGCTAAAATTATGGTATAATGTCAGTTGATAGCTGACAGGACTCAGCTTGGGAATGGGTATTGCCCTAGTCCCTGA
TTACAAAGGTTTCATTAGCC

Downstream 100 bases:

>100_bases
TTGGGCTTTTAGATGACAATATCCCGTATGGGTCTTGCTAATAGATTGATTAGCAGGAGCATACGGGATTTTTGGTGTCT
TAAAAACGAAATAAACAACA

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 672; Mature: 672

Protein sequence:

>672_residues
MKGRKMTKKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGYDVFYLTGLDEHGQKIQDKAQEAGLSPQAYVDG
MAEEVKALWKLLDISYDKFIRTTDDYHEEVVAAVFERLLAQDDIYLGTYSGWYSVSDEEFFTESQLAEIYRDEAGKVIGG
VAPSGHEVEWVSEESYFLRLSKYADRLVAFFKAQPDFIQPDGRMNEMLKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVV
YVWIDALLNYATALGYGQKDHANFDKFWSGRVFHMVGKDILRFHSIYWPILLMMLDLPMPERLIAHGWFVMKDGKMSKSK
GNVIYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGRVPAYVKNVTAF
DADLEQVVADHISEYHKQMEAVDYPRALEAVWTIISRTNKYIDETAPWVLAKAADSKEQLASVMAHLAASLRVVAHLIWP
FMMTTSAAMMEQLGLDATYHLEELDLADLPTGVTVVAKGEPIFPRLDMEAEIDYIKEQMKMSTAKPQEADWDPEKVALKS
EKDTITFEAFDAVEIRVAEVKEVSRVEGSEKLLRFRLDAGDGSDRQILSGIAAYYPNEQELVGKKVQIVANLKPRKMMKK
YISQGMLLSAEHDGKLTVLTVDPSVPNGAIIG

Sequences:

>Translated_672_residues
MKGRKMTKKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGYDVFYLTGLDEHGQKIQDKAQEAGLSPQAYVDG
MAEEVKALWKLLDISYDKFIRTTDDYHEEVVAAVFERLLAQDDIYLGTYSGWYSVSDEEFFTESQLAEIYRDEAGKVIGG
VAPSGHEVEWVSEESYFLRLSKYADRLVAFFKAQPDFIQPDGRMNEMLKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVV
YVWIDALLNYATALGYGQKDHANFDKFWSGRVFHMVGKDILRFHSIYWPILLMMLDLPMPERLIAHGWFVMKDGKMSKSK
GNVIYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGRVPAYVKNVTAF
DADLEQVVADHISEYHKQMEAVDYPRALEAVWTIISRTNKYIDETAPWVLAKAADSKEQLASVMAHLAASLRVVAHLIWP
FMMTTSAAMMEQLGLDATYHLEELDLADLPTGVTVVAKGEPIFPRLDMEAEIDYIKEQMKMSTAKPQEADWDPEKVALKS
EKDTITFEAFDAVEIRVAEVKEVSRVEGSEKLLRFRLDAGDGSDRQILSGIAAYYPNEQELVGKKVQIVANLKPRKMMKK
YISQGMLLSAEHDGKLTVLTVDPSVPNGAIIG
>Mature_672_residues
MKGRKMTKKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGYDVFYLTGLDEHGQKIQDKAQEAGLSPQAYVDG
MAEEVKALWKLLDISYDKFIRTTDDYHEEVVAAVFERLLAQDDIYLGTYSGWYSVSDEEFFTESQLAEIYRDEAGKVIGG
VAPSGHEVEWVSEESYFLRLSKYADRLVAFFKAQPDFIQPDGRMNEMLKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVV
YVWIDALLNYATALGYGQKDHANFDKFWSGRVFHMVGKDILRFHSIYWPILLMMLDLPMPERLIAHGWFVMKDGKMSKSK
GNVIYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYVGRINYELANDLGNLLNRTVAMINKYFDGRVPAYVKNVTAF
DADLEQVVADHISEYHKQMEAVDYPRALEAVWTIISRTNKYIDETAPWVLAKAADSKEQLASVMAHLAASLRVVAHLIWP
FMMTTSAAMMEQLGLDATYHLEELDLADLPTGVTVVAKGEPIFPRLDMEAEIDYIKEQMKMSTAKPQEADWDPEKVALKS
EKDTITFEAFDAVEIRVAEVKEVSRVEGSEKLLRFRLDAGDGSDRQILSGIAAYYPNEQELVGKKVQIVANLKPRKMMKK
YISQGMLLSAEHDGKLTVLTVDPSVPNGAIIG

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI24308436, Length=553, Percent_Identity=32.5497287522604, Blast_Score=280, Evalue=5e-75,
Organism=Homo sapiens, GI14043022, Length=568, Percent_Identity=26.9366197183099, Blast_Score=180, Evalue=3e-45,
Organism=Escherichia coli, GI1788432, Length=683, Percent_Identity=28.696925329429, Blast_Score=273, Evalue=3e-74,
Organism=Caenorhabditis elegans, GI212645096, Length=506, Percent_Identity=31.0276679841897, Blast_Score=252, Evalue=4e-67,
Organism=Caenorhabditis elegans, GI17540794, Length=556, Percent_Identity=26.0791366906475, Blast_Score=182, Evalue=5e-46,
Organism=Caenorhabditis elegans, GI71992781, Length=389, Percent_Identity=30.3341902313625, Blast_Score=179, Evalue=5e-45,
Organism=Saccharomyces cerevisiae, GI6321610, Length=509, Percent_Identity=32.2200392927308, Blast_Score=268, Evalue=2e-72,
Organism=Saccharomyces cerevisiae, GI6321704, Length=558, Percent_Identity=24.5519713261649, Blast_Score=150, Evalue=5e-37,
Organism=Drosophila melanogaster, GI21358483, Length=499, Percent_Identity=32.8657314629259, Blast_Score=251, Evalue=2e-66,
Organism=Drosophila melanogaster, GI24655311, Length=556, Percent_Identity=25.8992805755396, Blast_Score=172, Evalue=1e-42,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 76175; Mature: 76175

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
4.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKGRKMTKKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGYDVFYLTGLDEHG
CCCCCCCCCCCEEEECCEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHH
QKIQDKAQEAGLSPQAYVDGMAEEVKALWKLLDISYDKFIRTTDDYHEEVVAAVFERLLA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHC
QDDIYLGTYSGWYSVSDEEFFTESQLAEIYRDEAGKVIGGVAPSGHEVEWVSEESYFLRL
CCCEEEEECCCCEECCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEECCCHHHHHH
SKYADRLVAFFKAQPDFIQPDGRMNEMLKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEE
YVWIDALLNYATALGYGQKDHANFDKFWSGRVFHMVGKDILRFHSIYWPILLMMLDLPMP
EHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ERLIAHGWFVMKDGKMSKSKGNVIYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYV
HHHHHCCCEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHH
GRINYELANDLGNLLNRTVAMINKYFDGRVPAYVKNVTAFDADLEQVVADHISEYHKQME
HHEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVDYPRALEAVWTIISRTNKYIDETAPWVLAKAADSKEQLASVMAHLAASLRVVAHLIWP
HHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
FMMTTSAAMMEQLGLDATYHLEELDLADLPTGVTVVAKGEPIFPRLDMEAEIDYIKEQMK
HHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
MSTAKPQEADWDPEKVALKSEKDTITFEAFDAVEIRVAEVKEVSRVEGSEKLLRFRLDAG
HHCCCCCCCCCCHHHEEECCCCCEEEEEECCCCEEEHHHHHHHHHCCCHHHEEEEEECCC
DGSDRQILSGIAAYYPNEQELVGKKVQIVANLKPRKMMKKYISQGMLLSAEHDGKLTVLT
CCCHHHHHHHHHHHCCCHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCEEEEEE
VDPSVPNGAIIG
ECCCCCCCCCCC
>Mature Secondary Structure
MKGRKMTKKKPFYITTPIYYPSGKLHIGSAYTTIACDVLARYKRMMGYDVFYLTGLDEHG
CCCCCCCCCCCEEEECCEEECCCCEEECCHHHHHHHHHHHHHHHHHCCCEEEEECCCHHH
QKIQDKAQEAGLSPQAYVDGMAEEVKALWKLLDISYDKFIRTTDDYHEEVVAAVFERLLA
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHCCCHHHHHHHHHHHHHHHHC
QDDIYLGTYSGWYSVSDEEFFTESQLAEIYRDEAGKVIGGVAPSGHEVEWVSEESYFLRL
CCCEEEEECCCCEECCCHHHHHHHHHHHHHHHHCCCEEECCCCCCCCEEEECCCHHHHHH
SKYADRLVAFFKAQPDFIQPDGRMNEMLKNFIEPGLEDLAVSRTTFTWGVPVPSDPKHVV
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHEECEEEEECCCCCCCCCEEE
YVWIDALLNYATALGYGQKDHANFDKFWSGRVFHMVGKDILRFHSIYWPILLMMLDLPMP
EHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
ERLIAHGWFVMKDGKMSKSKGNVIYPEMLVERFGLDPLRYYLMRSLPVGSDGTFTPEDYV
HHHHHCCCEEEECCCCCCCCCCEECHHHHHHHHCCCHHHHHHHHHCCCCCCCCCCCHHHH
GRINYELANDLGNLLNRTVAMINKYFDGRVPAYVKNVTAFDADLEQVVADHISEYHKQME
HHEHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AVDYPRALEAVWTIISRTNKYIDETAPWVLAKAADSKEQLASVMAHLAASLRVVAHLIWP
HHCCHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHH
FMMTTSAAMMEQLGLDATYHLEELDLADLPTGVTVVAKGEPIFPRLDMEAEIDYIKEQMK
HHHHHHHHHHHHHCCCCEEEHHHCCCCCCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHH
MSTAKPQEADWDPEKVALKSEKDTITFEAFDAVEIRVAEVKEVSRVEGSEKLLRFRLDAG
HHCCCCCCCCCCHHHEEECCCCCEEEEEECCCCEEEHHHHHHHHHCCCHHHEEEEEECCC
DGSDRQILSGIAAYYPNEQELVGKKVQIVANLKPRKMMKKYISQGMLLSAEHDGKLTVLT
CCCHHHHHHHHHHHCCCHHHHCCCCEEEEECCCHHHHHHHHHCCCEEEEECCCCEEEEEE
VDPSVPNGAIIG
ECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]