Click here to switch to the map view.

The map label for this gene is ogt [H]

Identifier: 195977649

GI number: 195977649

Start: 526511

End: 526987

Strand: Direct

Name: ogt [H]

Synonym: Sez_0508

Alternate gene names: 195977649

Gene position: 526511-526987 (Clockwise)

Preceding gene: 195977648

Following gene: 195977650

Centisome position: 26.01

GC content: 48.01

Gene sequence:

>477_bases
GTGTATCAATCGCCCTTAGGAAAAATATCCTTGGTTGCGACTGATGTTGCTTTAATAGGAGCCTGGTTTGAAGGTCAAAG
GTATTTTCAGGCTGGGATTAAAGTGCAGCCCATCTGGCAGGAGCAGCATGCCCTGTTAAAAAGAGCCTGTTTGTGGCTTG
ATGCTTACTTTGAGGGGAAGCCCTATCCCATGCTTGATTGCTTATCTCCACAGGGTACTGATTTTCAGCGAGCTGTTTGG
CGAGAATTGCAGACGATAAGATGGGGAGAGACCTTGAGCTATGGTGAGCTTGCCAAGCGCCTGCAGTGTCGTTCGGCTCA
GGCTGTTGGTGGTGCCATAGGACGTAATCCTTTAAGTATTTTTATTCCCTGTCATCGTGTACTGGCTGCTGATGGTCGCT
TGATTGGCTATGCTGGAGGACTTGAAAAGAAGGCCTGGTTGCTCAGGCACGAGCAAATTAGTGTTAATAAGGAGTAG

Upstream 100 bases:

>100_bases
AGGGCTCCTTCTTGCCTTTAGCTAGGCTTTTTCTTATAATAAATAGTCAAGGGAATAAGCTATTATGTGGGGAGGTCAGA
TCTTGCCATTGTACGAGTGT

Downstream 100 bases:

>100_bases
CTGATGATTACCATTTACCAATACCCAAAGTGTAGTACCTGTCAAAAAGCCCTTGCTGAGCTAAAGACCTTGGTTTCTGA
GGTTGATATCATTGATATTA

Product: methylated-DNA--protein-cysteine methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 158; Mature: 158

Protein sequence:

>158_residues
MYQSPLGKISLVATDVALIGAWFEGQRYFQAGIKVQPIWQEQHALLKRACLWLDAYFEGKPYPMLDCLSPQGTDFQRAVW
RELQTIRWGETLSYGELAKRLQCRSAQAVGGAIGRNPLSIFIPCHRVLAADGRLIGYAGGLEKKAWLLRHEQISVNKE

Sequences:

>Translated_158_residues
MYQSPLGKISLVATDVALIGAWFEGQRYFQAGIKVQPIWQEQHALLKRACLWLDAYFEGKPYPMLDCLSPQGTDFQRAVW
RELQTIRWGETLSYGELAKRLQCRSAQAVGGAIGRNPLSIFIPCHRVLAADGRLIGYAGGLEKKAWLLRHEQISVNKE
>Mature_158_residues
MYQSPLGKISLVATDVALIGAWFEGQRYFQAGIKVQPIWQEQHALLKRACLWLDAYFEGKPYPMLDCLSPQGTDFQRAVW
RELQTIRWGETLSYGELAKRLQCRSAQAVGGAIGRNPLSIFIPCHRVLAADGRLIGYAGGLEKKAWLLRHEQISVNKE

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=114, Percent_Identity=42.1052631578947, Blast_Score=82, Evalue=3e-16,
Organism=Escherichia coli, GI1787596, Length=102, Percent_Identity=49.0196078431373, Blast_Score=97, Evalue=4e-22,
Organism=Escherichia coli, GI1788542, Length=98, Percent_Identity=43.8775510204082, Blast_Score=78, Evalue=2e-16,
Organism=Saccharomyces cerevisiae, GI6320001, Length=82, Percent_Identity=41.4634146341463, Blast_Score=76, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 17846; Mature: 17846

Theoretical pI: Translated: 9.20; Mature: 9.20

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQSPLGKISLVATDVALIGAWFEGQRYFQAGIKVQPIWQEQHALLKRACLWLDAYFEGK
CCCCCCCCEEHHHHHHHHHHHHHCCCHHHHCCCEEEECHHHHHHHHHHHHHHHHHHCCCC
PYPMLDCLSPQGTDFQRAVWRELQTIRWGETLSYGELAKRLQCRSAQAVGGAIGRNPLSI
CCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
FIPCHRVLAADGRLIGYAGGLEKKAWLLRHEQISVNKE
EECHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MYQSPLGKISLVATDVALIGAWFEGQRYFQAGIKVQPIWQEQHALLKRACLWLDAYFEGK
CCCCCCCCEEHHHHHHHHHHHHHCCCHHHHCCCEEEECHHHHHHHHHHHHHHHHHHCCCC
PYPMLDCLSPQGTDFQRAVWRELQTIRWGETLSYGELAKRLQCRSAQAVGGAIGRNPLSI
CCCEEECCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEE
FIPCHRVLAADGRLIGYAGGLEKKAWLLRHEQISVNKE
EECHHHHHHCCCCEEEECCCCCHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]