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The map label for this gene is rbsB [H]

Identifier: 195977614

GI number: 195977614

Start: 493904

End: 494872

Strand: Direct

Name: rbsB [H]

Synonym: Sez_0473

Alternate gene names: 195977614

Gene position: 493904-494872 (Clockwise)

Preceding gene: 195977613

Following gene: 195977615

Centisome position: 24.4

GC content: 43.14

Gene sequence:

>969_bases
ATGACATTTGGTAAAAAACTAGGCTTTTTAGCCCTTATTCTCTCAATGGTGCTTGTACTTGGTGCTTGTGGCAAGACTGG
TCTAGGTGGGTCATCATCAGAATCAACCAAAGAGACCACTAAAAAGGAAGCCAAGGATTTGAAGCTCGGAGTTTCCATTT
CGACAACTAACAATCCTTATTTTGTAGCCATGAAGGACGGTCTTGATAAGGCTGCCAAGAAAACTGGTGTCGCTATCAAG
GTTGCAGACGCTCAAGACGATGCTGCTCGTCAAGCAGATGATGTGCAAAACTTTATCAGTCAACGGGTGGATGCCCTTCT
CATCAATCCAGTTGATTCAGATGCGATTGTCCCCGTTATTAAGGCAGCAAATACTGCTCATATCCCAGTCATCTTGATTG
ACCGAGGTAGCAATGGCGGTGAGGTCTTGACAACCGTTGCTTCTGACAACGTAGAAGCAGGTAAAATGGCAGCTGATTTT
ATTGTCAAAAAGCTTGGTGATAAGGCCAAGACTTTTGAATTGTCAGGTGTTCCAGGAGCTTCAGCTACTGTTGATAGAGG
CAATGGCTTTAATAGCATTGCAAAAGAACACTTGACAGTTTTGTCTAGTCAGTCAGCAGGCTTTGATCGTGCAAAAGCTC
TTAATACAGCTCAAAACATGATACAGGCTAATAAAGATGTTCAAGTTATTTTTGCTCAAAATGATGAAATGGCGCTAGGA
GCTGCTCAGGCTGTGAAGGCTGCTGGTCTGAAAAATGTCCTTATTGTTGGTATTGATGGTCAGCCAGATGCTCATCAGGC
TATTGAAAAAGGTGATCTCACAGCCACTATTGCTCAACAGCCGGCCAAGATGGGTGAGATTGCCATTCAAGCAGCTGTTG
ACCACTATCAGGGTAAGGCTATTGACAAGGAAACAGTATCTCCTATCTACCTTGTAACAAAAGAAAATGTTGATAAGTAC
AACTGGTAA

Upstream 100 bases:

>100_bases
GTGTGTCTGCATTCTGGCAGCAGGTTGTCAAGGGGATCGTTATTCTGATTGCTGTCTTGCTTGATCGTTTTAAAGTAGCA
AAATAAAAGGAGGCTAACAA

Downstream 100 bases:

>100_bases
TAGAGCTAGTAGCTATCTAGCAGACCCTTTTAGAGAAGGCTTGCGAACTTAGCTGGTCAGCAGAAGCATCAAAAATAGCT
GCTTGTCTGGTGGACAGGTA

Product: D-ribose-binding protein precursor RbsB

Products: ADP; phosphate; ribose [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 322; Mature: 321

Protein sequence:

>322_residues
MTFGKKLGFLALILSMVLVLGACGKTGLGGSSSESTKETTKKEAKDLKLGVSISTTNNPYFVAMKDGLDKAAKKTGVAIK
VADAQDDAARQADDVQNFISQRVDALLINPVDSDAIVPVIKAANTAHIPVILIDRGSNGGEVLTTVASDNVEAGKMAADF
IVKKLGDKAKTFELSGVPGASATVDRGNGFNSIAKEHLTVLSSQSAGFDRAKALNTAQNMIQANKDVQVIFAQNDEMALG
AAQAVKAAGLKNVLIVGIDGQPDAHQAIEKGDLTATIAQQPAKMGEIAIQAAVDHYQGKAIDKETVSPIYLVTKENVDKY
NW

Sequences:

>Translated_322_residues
MTFGKKLGFLALILSMVLVLGACGKTGLGGSSSESTKETTKKEAKDLKLGVSISTTNNPYFVAMKDGLDKAAKKTGVAIK
VADAQDDAARQADDVQNFISQRVDALLINPVDSDAIVPVIKAANTAHIPVILIDRGSNGGEVLTTVASDNVEAGKMAADF
IVKKLGDKAKTFELSGVPGASATVDRGNGFNSIAKEHLTVLSSQSAGFDRAKALNTAQNMIQANKDVQVIFAQNDEMALG
AAQAVKAAGLKNVLIVGIDGQPDAHQAIEKGDLTATIAQQPAKMGEIAIQAAVDHYQGKAIDKETVSPIYLVTKENVDKY
NW
>Mature_321_residues
TFGKKLGFLALILSMVLVLGACGKTGLGGSSSESTKETTKKEAKDLKLGVSISTTNNPYFVAMKDGLDKAAKKTGVAIKV
ADAQDDAARQADDVQNFISQRVDALLINPVDSDAIVPVIKAANTAHIPVILIDRGSNGGEVLTTVASDNVEAGKMAADFI
VKKLGDKAKTFELSGVPGASATVDRGNGFNSIAKEHLTVLSSQSAGFDRAKALNTAQNMIQANKDVQVIFAQNDEMALGA
AQAVKAAGLKNVLIVGIDGQPDAHQAIEKGDLTATIAQQPAKMGEIAIQAAVDHYQGKAIDKETVSPIYLVTKENVDKYN
W

Specific function: Involved in the high-affinity D-ribose membrane transport system [H]

COG id: COG1879

COG function: function code G; ABC-type sugar transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 2 family [H]

Homologues:

Organism=Escherichia coli, GI1790192, Length=273, Percent_Identity=45.4212454212454, Blast_Score=231, Evalue=5e-62,
Organism=Escherichia coli, GI1790674, Length=226, Percent_Identity=36.7256637168142, Blast_Score=141, Evalue=7e-35,
Organism=Escherichia coli, GI1790526, Length=276, Percent_Identity=32.6086956521739, Blast_Score=124, Evalue=7e-30,
Organism=Escherichia coli, GI1788473, Length=291, Percent_Identity=26.1168384879725, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1789990, Length=295, Percent_Identity=26.1016949152542, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: 3940 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 5900 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 1520 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001761 [H]

Pfam domain/function: PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 33737; Mature: 33606

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFGKKLGFLALILSMVLVLGACGKTGLGGSSSESTKETTKKEAKDLKLGVSISTTNNPY
CCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCE
FVAMKDGLDKAAKKTGVAIKVADAQDDAARQADDVQNFISQRVDALLINPVDSDAIVPVI
EEEECCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHE
KAANTAHIPVILIDRGSNGGEVLTTVASDNVEAGKMAADFIVKKLGDKAKTFELSGVPGA
ECCCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCC
SATVDRGNGFNSIAKEHLTVLSSQSAGFDRAKALNTAQNMIQANKDVQVIFAQNDEMALG
CCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
AAQAVKAAGLKNVLIVGIDGQPDAHQAIEKGDLTATIAQQPAKMGEIAIQAAVDHYQGKA
HHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCEEHHHHCCHHHHHHHHHHHHHHHCCCC
IDKETVSPIYLVTKENVDKYNW
CCCCCCCCEEEEEECCCCCCCC
>Mature Secondary Structure 
TFGKKLGFLALILSMVLVLGACGKTGLGGSSSESTKETTKKEAKDLKLGVSISTTNNPY
CCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHEEEEEEEEECCCCE
FVAMKDGLDKAAKKTGVAIKVADAQDDAARQADDVQNFISQRVDALLINPVDSDAIVPVI
EEEECCCHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHCCEEEECCCCCCCHHHHE
KAANTAHIPVILIDRGSNGGEVLTTVASDNVEAGKMAADFIVKKLGDKAKTFELSGVPGA
ECCCCCCEEEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCC
SATVDRGNGFNSIAKEHLTVLSSQSAGFDRAKALNTAQNMIQANKDVQVIFAQNDEMALG
CCEEECCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHH
AAQAVKAAGLKNVLIVGIDGQPDAHQAIEKGDLTATIAQQPAKMGEIAIQAAVDHYQGKA
HHHHHHHCCCCEEEEEEECCCCCHHHHHHHCCCEEHHHHCCHHHHHHHHHHHHHHHCCCC
IDKETVSPIYLVTKENVDKYNW
CCCCCCCCEEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; ribose [Periplasm]; H2O [C]

Specific reaction: ATP + ribose [Periplasm] + H2O = ADP + phosphate + ribose [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9353933; 9384377; 7921236 [H]