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The map label for this gene is grpE [H]

Identifier: 195977544

GI number: 195977544

Start: 429260

End: 429829

Strand: Direct

Name: grpE [H]

Synonym: Sez_0401

Alternate gene names: 195977544

Gene position: 429260-429829 (Clockwise)

Preceding gene: 195977543

Following gene: 195977545

Centisome position: 21.21

GC content: 45.79

Gene sequence:

>570_bases
TTGTCTAAAAAACCAAAAGAAGAGCTCAAGGATCAAGAGGACATTCTTGATGAGGCTGCTGAGGAAGCAGTAGAGCCAGT
TGAAGAGGCTGATCAATCAGCTGATACTGTTGCTGATGAAGCAGCCAAAGAGCTATCTGAGCTAGAGCAGGCTCTCCAGC
GAGCAGAGGATTTTGAAAATAAATACCTCAGAGCTCACGCAGAAATGCAAAATATTCAGCGTCGTGCTAATGAAGAACGC
CAAAGCTTGCAGCGCTATCGCTCACAGGATTTAGCCAAAAAAATCCTTCCAAGCTTAGATAACTTGGAGCGTGCTTTAGC
TGTTGATGGTCTGACAGATGATGTCAAAAAAGGCCTAGAAATGGTGCAAGAGAGCCTTGTTCAAGCCCTCAAAGAAGAAG
GCATTGAAGAGGTTCCTGTGGAAGCCTTTGACCACAACCTGCACATGGCAGTCCAAACCCTACCAGCAGATGATGACCAC
CCAGCAGATAGTATTGCTCAGGTTTTCCAAAAAGGCTATAAGCTTCATGAGCGCTTACTAAGACCAGCGATGGTGGTCGT
ATACAACTAG

Upstream 100 bases:

>100_bases
TGAAGCTAAGAGATTATTACCGGTACTTAAACAGCAACCATTATGAGGTTCACTAAGGCCTTAATATGATTTAAGAAAAT
GTAAGAAAGGGGTAGACACC

Downstream 100 bases:

>100_bases
TCACTAATGATGGCTATTAAAATGGCTCATCATTAGACGGCAACCGCTGTGGCGGCTTGCCTAGACTTCTTACTAGTTCG
ATAGCAAAATGGTATCGATT

Product: heat shock protein GrpE

Products: NA

Alternate protein names: HSP-70 cofactor [H]

Number of amino acids: Translated: 189; Mature: 188

Protein sequence:

>189_residues
MSKKPKEELKDQEDILDEAAEEAVEPVEEADQSADTVADEAAKELSELEQALQRAEDFENKYLRAHAEMQNIQRRANEER
QSLQRYRSQDLAKKILPSLDNLERALAVDGLTDDVKKGLEMVQESLVQALKEEGIEEVPVEAFDHNLHMAVQTLPADDDH
PADSIAQVFQKGYKLHERLLRPAMVVVYN

Sequences:

>Translated_189_residues
MSKKPKEELKDQEDILDEAAEEAVEPVEEADQSADTVADEAAKELSELEQALQRAEDFENKYLRAHAEMQNIQRRANEER
QSLQRYRSQDLAKKILPSLDNLERALAVDGLTDDVKKGLEMVQESLVQALKEEGIEEVPVEAFDHNLHMAVQTLPADDDH
PADSIAQVFQKGYKLHERLLRPAMVVVYN
>Mature_188_residues
SKKPKEELKDQEDILDEAAEEAVEPVEEADQSADTVADEAAKELSELEQALQRAEDFENKYLRAHAEMQNIQRRANEERQ
SLQRYRSQDLAKKILPSLDNLERALAVDGLTDDVKKGLEMVQESLVQALKEEGIEEVPVEAFDHNLHMAVQTLPADDDHP
ADSIAQVFQKGYKLHERLLRPAMVVVYN

Specific function: Participates actively in the response to hyperosmotic and heat shock by preventing the aggregation of stress-denatured proteins, in association with dnaK and grpE. It is the nucleotide exchange factor for dnaK and may function as a thermosensor. Unfolded

COG id: COG0576

COG function: function code O; Molecular chaperone GrpE (heat shock protein)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the grpE family [H]

Homologues:

Organism=Escherichia coli, GI1788967, Length=187, Percent_Identity=32.0855614973262, Blast_Score=75, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324806, Length=152, Percent_Identity=30.2631578947368, Blast_Score=66, Evalue=4e-12,
Organism=Drosophila melanogaster, GI24653432, Length=173, Percent_Identity=30.0578034682081, Blast_Score=67, Evalue=6e-12,

Paralogues:

None

Copy number: 2359 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000740
- InterPro:   IPR013805
- InterPro:   IPR009012 [H]

Pfam domain/function: PF01025 GrpE [H]

EC number: NA

Molecular weight: Translated: 21476; Mature: 21345

Theoretical pI: Translated: 4.28; Mature: 4.28

Prosite motif: PS01071 GRPE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKKPKEELKDQEDILDEAAEEAVEPVEEADQSADTVADEAAKELSELEQALQRAEDFEN
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYLRAHAEMQNIQRRANEERQSLQRYRSQDLAKKILPSLDNLERALAVDGLTDDVKKGLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHH
MVQESLVQALKEEGIEEVPVEAFDHNLHMAVQTLPADDDHPADSIAQVFQKGYKLHERLL
HHHHHHHHHHHHCCCHHCCHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
RPAMVVVYN
HHHHHHCCC
>Mature Secondary Structure 
SKKPKEELKDQEDILDEAAEEAVEPVEEADQSADTVADEAAKELSELEQALQRAEDFEN
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KYLRAHAEMQNIQRRANEERQSLQRYRSQDLAKKILPSLDNLERALAVDGLTDDVKKGLE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCHHHHHHHHH
MVQESLVQALKEEGIEEVPVEAFDHNLHMAVQTLPADDDHPADSIAQVFQKGYKLHERLL
HHHHHHHHHHHHCCCHHCCHHHHHCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
RPAMVVVYN
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA