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The map label for this gene is recG [H]

Identifier: 195977526

GI number: 195977526

Start: 408472

End: 410487

Strand: Direct

Name: recG [H]

Synonym: Sez_0381

Alternate gene names: 195977526

Gene position: 408472-410487 (Clockwise)

Preceding gene: 195977525

Following gene: 195977528

Centisome position: 20.18

GC content: 45.88

Gene sequence:

>2016_bases
ATGCTGTTAAAGAGTTCAATTGCTAATTTGAAGGGGCTTGGTCCAAAGTCAGCTGAGAAATTTCAAAAATTAGGCATTTA
TCAGATCAAGGACTTGCTGCTCTATTATCCTTTTCGCTATGAGGATTTTAAGAGCAAGTCAGCCTTAGAGCTTTTAGATG
GTGAAAAGGCTGTCCTTACAGGTAGAGTTGTGACCCCACCAAGCGTTCAATACTATGGGTTCAAGCGTAATCGCCTGAGC
TTTAAATTGAAGCAAGGGGAGCTTGTGATTAGTGTCAGCTTTTTCAATCAGCCTTACCTACTTGATAAAATCGTCTTGGA
TCAGGAGGTGGCTATCTTTGGCAGGTGGGATCAAAGAAAGGCGGCCTTGACAGGCATGAAATTACTCATGACCTTAGAAG
ATGATTTGCAGCCAGTTTATCATGTGGCGCAGGGGATTAGTCAGCCCGCCCTGATTAAGGCGATCAGATCAGCATTTGAT
AGTGGTGCCTTAGCTCAGCTGGAAGAAAATTTACCAGAGCCCTTGATTCAGAAATACCGCCTGCTGCCGAGACAAGCTGC
TGTTTATGCCATGCATTTTCCTAAACATATGGAGGATTATAAGCAGGCCCTGCGCCGTATAAAATTTGAAGAATTTTTCT
ATTTTCAGATGAATCTGCAGGTTTTAAAGCTTGCTAACAAGGCAGAGGGCAATGGGTTAGCGATTCCTTTTCATCAGCAG
GCAGTGGCAAAAAAGATTGCTGCTTTGCCCTTTTCACTGACCAGAGCTCAACAAAGAAGCCTGGAGGAAATCTTAGCAGA
TATGACCTCAGGGGCTCATATGAACCGTCTGTTGCAGGGGGATGTCGGCTCTGGCAAGACAGTGATCGCAAGCCTTGCCA
TGTATGCTGCCCAAACAGCGGGATTTCAATCGGCACTCATGGTGCCAACTGAGATTTTGGCTGAGCAACATTACGATAGC
CTTTGTCAGCTGTTTCCAGATTTGTCTATTGCTCTATTGACATCGGGTATGAAAGTAGCTGAAAAAAGAACAGCTTTATC
AGCTATTGCTGATGGGTCTGTAGATATGATTGTTGGGACACATGCCTTGATCCAGGACACAGTAACCTATCATAAGCTAG
GGCTAGTGATTACTGATGAGCAGCACCGCTTTGGGGTCAATCAGCGTCGCTTGTTTCGCGAAAAAGGCAACAATCCAGAT
GTTCTGATGATGACAGCAACTCCCATACCTAGAACCCTAGCCATAACCGCCTATGGTGAGATGGACGTTTCCATTATTGA
TGAATTACCGGCAGGGCGAAAGCCAATCATCACTCGCTGGGTCAAGCATCAGCAATTAGATAGTGTGCTGGCCTGGGTAA
AGACCGAGCTTGCTAAGGGGGCACAGGCCTATGTTATCTCTCCCTTAATCGAAGAATCTGAAACTCTGGATTTGAAAAAT
GCGGTAGCTTTAGAGGCTGAGCTGAAGGATTATTTTAAAGCTTCAGCTAGCATTGCCTTGATGCATGGCAAAATGAAGGC
AGACGACAAGGAGATGATCATGCAGCGCTTTAAGGCTAAGGAGATTGATGTCTTGGTGTCAACAACCGTTATTGAGGTTG
GTGTCAATGTGCCAAATGCCACCATCATGCTGATCATGAATGCTGACCGCTTTGGTCTTAGCCAATTGCATCAGCTGCGC
GGTCGTGTTGGGCGAGGCAGCAAGCAATCCTATGCTATTTTGGTGGCAGATCCTAAGACAGACTCAGGCAAGGAGCGTAT
GACCATCATGACAACGACTAATGATGGCTTTGTGCTGGCAGAGGCTGACCTCAAGCTGCGTGGCTCAGGAGAGATTTTTG
GGACCCGTCAATCAGGTTTACCAGAGTTCAAAACAGCTGATATTGTAGAGGATTATCATATCCTAGAGGAGGCAAGACGA
GTAGCTACTGAGATTGTTGCAGCTAAGAATTGGCAGCTAGACCCTAGATGGGCTCCGATTTTGGCTAATCTTCGTGTCGC
TGATCAGTTTGATTAG

Upstream 100 bases:

>100_bases
GCTAATACCTCAAGGCACCTACAATAAGAAAACGAGCCTCTTCCTTTTTGGAGAGGCTCTTGATTTTTGATATAATAATG
AAAAAGAAAGCGAGGTTGAG

Downstream 100 bases:

>100_bases
ATGTGAGGCTGACTTGCTGGACAGAGCTAATATAAAAACACGACAAGAGGTTCTTAGCTGACCGAGTCGTGCTTTTTGCG
CTATAGATGAGAAGTAGTAG

Product: ATP-dependent DNA helicase RecG

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 671; Mature: 671

Protein sequence:

>671_residues
MLLKSSIANLKGLGPKSAEKFQKLGIYQIKDLLLYYPFRYEDFKSKSALELLDGEKAVLTGRVVTPPSVQYYGFKRNRLS
FKLKQGELVISVSFFNQPYLLDKIVLDQEVAIFGRWDQRKAALTGMKLLMTLEDDLQPVYHVAQGISQPALIKAIRSAFD
SGALAQLEENLPEPLIQKYRLLPRQAAVYAMHFPKHMEDYKQALRRIKFEEFFYFQMNLQVLKLANKAEGNGLAIPFHQQ
AVAKKIAALPFSLTRAQQRSLEEILADMTSGAHMNRLLQGDVGSGKTVIASLAMYAAQTAGFQSALMVPTEILAEQHYDS
LCQLFPDLSIALLTSGMKVAEKRTALSAIADGSVDMIVGTHALIQDTVTYHKLGLVITDEQHRFGVNQRRLFREKGNNPD
VLMMTATPIPRTLAITAYGEMDVSIIDELPAGRKPIITRWVKHQQLDSVLAWVKTELAKGAQAYVISPLIEESETLDLKN
AVALEAELKDYFKASASIALMHGKMKADDKEMIMQRFKAKEIDVLVSTTVIEVGVNVPNATIMLIMNADRFGLSQLHQLR
GRVGRGSKQSYAILVADPKTDSGKERMTIMTTTNDGFVLAEADLKLRGSGEIFGTRQSGLPEFKTADIVEDYHILEEARR
VATEIVAAKNWQLDPRWAPILANLRVADQFD

Sequences:

>Translated_671_residues
MLLKSSIANLKGLGPKSAEKFQKLGIYQIKDLLLYYPFRYEDFKSKSALELLDGEKAVLTGRVVTPPSVQYYGFKRNRLS
FKLKQGELVISVSFFNQPYLLDKIVLDQEVAIFGRWDQRKAALTGMKLLMTLEDDLQPVYHVAQGISQPALIKAIRSAFD
SGALAQLEENLPEPLIQKYRLLPRQAAVYAMHFPKHMEDYKQALRRIKFEEFFYFQMNLQVLKLANKAEGNGLAIPFHQQ
AVAKKIAALPFSLTRAQQRSLEEILADMTSGAHMNRLLQGDVGSGKTVIASLAMYAAQTAGFQSALMVPTEILAEQHYDS
LCQLFPDLSIALLTSGMKVAEKRTALSAIADGSVDMIVGTHALIQDTVTYHKLGLVITDEQHRFGVNQRRLFREKGNNPD
VLMMTATPIPRTLAITAYGEMDVSIIDELPAGRKPIITRWVKHQQLDSVLAWVKTELAKGAQAYVISPLIEESETLDLKN
AVALEAELKDYFKASASIALMHGKMKADDKEMIMQRFKAKEIDVLVSTTVIEVGVNVPNATIMLIMNADRFGLSQLHQLR
GRVGRGSKQSYAILVADPKTDSGKERMTIMTTTNDGFVLAEADLKLRGSGEIFGTRQSGLPEFKTADIVEDYHILEEARR
VATEIVAAKNWQLDPRWAPILANLRVADQFD
>Mature_671_residues
MLLKSSIANLKGLGPKSAEKFQKLGIYQIKDLLLYYPFRYEDFKSKSALELLDGEKAVLTGRVVTPPSVQYYGFKRNRLS
FKLKQGELVISVSFFNQPYLLDKIVLDQEVAIFGRWDQRKAALTGMKLLMTLEDDLQPVYHVAQGISQPALIKAIRSAFD
SGALAQLEENLPEPLIQKYRLLPRQAAVYAMHFPKHMEDYKQALRRIKFEEFFYFQMNLQVLKLANKAEGNGLAIPFHQQ
AVAKKIAALPFSLTRAQQRSLEEILADMTSGAHMNRLLQGDVGSGKTVIASLAMYAAQTAGFQSALMVPTEILAEQHYDS
LCQLFPDLSIALLTSGMKVAEKRTALSAIADGSVDMIVGTHALIQDTVTYHKLGLVITDEQHRFGVNQRRLFREKGNNPD
VLMMTATPIPRTLAITAYGEMDVSIIDELPAGRKPIITRWVKHQQLDSVLAWVKTELAKGAQAYVISPLIEESETLDLKN
AVALEAELKDYFKASASIALMHGKMKADDKEMIMQRFKAKEIDVLVSTTVIEVGVNVPNATIMLIMNADRFGLSQLHQLR
GRVGRGSKQSYAILVADPKTDSGKERMTIMTTTNDGFVLAEADLKLRGSGEIFGTRQSGLPEFKTADIVEDYHILEEARR
VATEIVAAKNWQLDPRWAPILANLRVADQFD

Specific function: Critical role in recombination and DNA repair. Help process Holliday junction intermediates to mature products by catalyzing branch migration. Has a DNA unwinding activity characteristic of a DNA helicase with a 3' to 5' polarity. RecG unwind branched dup

COG id: COG1200

COG function: function code LK; RecG-like helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367254, Length=689, Percent_Identity=39.0420899854862, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1787357, Length=403, Percent_Identity=37.2208436724566, Blast_Score=255, Evalue=8e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR004609
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR016027 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 74937; Mature: 74937

Theoretical pI: Translated: 9.06; Mature: 9.06

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLKSSIANLKGLGPKSAEKFQKLGIYQIKDLLLYYPFRYEDFKSKSALELLDGEKAVLT
CCCHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEEE
GRVVTPPSVQYYGFKRNRLSFKLKQGELVISVSFFNQPYLLDKIVLDQEVAIFGRWDQRK
EEEECCCCCEEEEEECCCEEEEEECCCEEEEEEECCCCHHHHHHHHCCCEEEEECCCHHH
AALTGMKLLMTLEDDLQPVYHVAQGISQPALIKAIRSAFDSGALAQLEENLPEPLIQKYR
HHHHHHHHEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHH
LLPRQAAVYAMHFPKHMEDYKQALRRIKFEEFFYFQMNLQVLKLANKAEGNGLAIPFHQQ
HCCHHHHHHHHHCHHHHHHHHHHHHHCCHHHEEEEEECHHEEEECCCCCCCCEEECCHHH
AVAKKIAALPFSLTRAQQRSLEEILADMTSGAHMNRLLQGDVGSGKTVIASLAMYAAQTA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
GFQSALMVPTEILAEQHYDSLCQLFPDLSIALLTSGMKVAEKRTALSAIADGSVDMIVGT
CCCHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECC
HALIQDTVTYHKLGLVITDEQHRFGVNQRRLFREKGNNPDVLMMTATPIPRTLAITAYGE
HHHHHHHHHHEEEEEEEECCHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECC
MDVSIIDELPAGRKPIITRWVKHQQLDSVLAWVKTELAKGAQAYVISPLIEESETLDLKN
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHCCCCCCCHHH
AVALEAELKDYFKASASIALMHGKMKADDKEMIMQRFKAKEIDVLVSTTVIEVGVNVPNA
HHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCHHEEEEEEEEEEECCCCCCE
TIMLIMNADRFGLSQLHQLRGRVGRGSKQSYAILVADPKTDSGKERMTIMTTTNDGFVLA
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCEEEE
EADLKLRGSGEIFGTRQSGLPEFKTADIVEDYHILEEARRVATEIVAAKNWQLDPRWAPI
ECCEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LANLRVADQFD
EECCEECCCCC
>Mature Secondary Structure
MLLKSSIANLKGLGPKSAEKFQKLGIYQIKDLLLYYPFRYEDFKSKSALELLDGEKAVLT
CCCHHHHHHHCCCCCCHHHHHHHCCHHHHHHHHHCCCCCCCCCCCCCHHHHHCCCCEEEE
GRVVTPPSVQYYGFKRNRLSFKLKQGELVISVSFFNQPYLLDKIVLDQEVAIFGRWDQRK
EEEECCCCCEEEEEECCCEEEEEECCCEEEEEEECCCCHHHHHHHHCCCEEEEECCCHHH
AALTGMKLLMTLEDDLQPVYHVAQGISQPALIKAIRSAFDSGALAQLEENLPEPLIQKYR
HHHHHHHHEEEHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCHHHHHHHCCCHHHHHHHH
LLPRQAAVYAMHFPKHMEDYKQALRRIKFEEFFYFQMNLQVLKLANKAEGNGLAIPFHQQ
HCCHHHHHHHHHCHHHHHHHHHHHHHCCHHHEEEEEECHHEEEECCCCCCCCEEECCHHH
AVAKKIAALPFSLTRAQQRSLEEILADMTSGAHMNRLLQGDVGSGKTVIASLAMYAAQTA
HHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHC
GFQSALMVPTEILAEQHYDSLCQLFPDLSIALLTSGMKVAEKRTALSAIADGSVDMIVGT
CCCHHHHCHHHHHHHHHHHHHHHHCCCCHHHHHHCCHHHHHHHHHHHHHCCCCEEEEECC
HALIQDTVTYHKLGLVITDEQHRFGVNQRRLFREKGNNPDVLMMTATPIPRTLAITAYGE
HHHHHHHHHHEEEEEEEECCHHHCCCHHHHHHHHCCCCCCEEEEECCCCCCEEEEEEECC
MDVSIIDELPAGRKPIITRWVKHQQLDSVLAWVKTELAKGAQAYVISPLIEESETLDLKN
CCHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECHHHCCCCCCCHHH
AVALEAELKDYFKASASIALMHGKMKADDKEMIMQRFKAKEIDVLVSTTVIEVGVNVPNA
HHHHHHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCHHEEEEEEEEEEECCCCCCE
TIMLIMNADRFGLSQLHQLRGRVGRGSKQSYAILVADPKTDSGKERMTIMTTTNDGFVLA
EEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCCCEEEEEEECCCCEEEE
EADLKLRGSGEIFGTRQSGLPEFKTADIVEDYHILEEARRVATEIVAAKNWQLDPRWAPI
ECCEEEECCCCEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
LANLRVADQFD
EECCEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8830261; 11463916 [H]