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The map label for this gene is nusB [H]

Identifier: 195977499

GI number: 195977499

Start: 374394

End: 374813

Strand: Direct

Name: nusB [H]

Synonym: Sez_0354

Alternate gene names: 195977499

Gene position: 374394-374813 (Clockwise)

Preceding gene: 195977498

Following gene: 195977500

Centisome position: 18.5

GC content: 43.1

Gene sequence:

>420_bases
TTGCGTGAGCGTGCTTTTCAAGCTTTATTTACTATGGAGATGGGCGGAGATTTCCTGCTTGCCTCACAGTTTGCCTATGA
CTATGACAAGGAAGTAGCTGACGACAAGCAGCCCTCTGAGTTGCCTGTTTTTCTGCTGAATCTTGTCAATGGCGTTATGG
ATCATAAGGCTGAGCTTGATGAGGTCATCAAGAAAAACCTCAAAGCAGGCTGGTCCATTGAACGCTTGACAGTGGTAGAC
AAGACAATGCTGCGTCTTGGGCTCTTTGAGATGACCTTGTTTGAGGAGACACCAGATCGTGTAGCCCTAAATGAAATCAT
CGAAATTGCTAAAAAATATTCTGATGATACTTCAGCTAAGTTTATTAATGGCTTACTTAGTCAGTTTGTTTCTGATGAGT
CAGTTGCAGTGACAGACTAA

Upstream 100 bases:

>100_bases
GAGGGGATCGTTCCTGAAAAGACACCAAAGCCAGAAATGAAATCATTGTTTGATGAGGATTTTTTGGATGACTAAGCGTT
TTCAACATTCGAGAAGAGAT

Downstream 100 bases:

>100_bases
CAAGCTAATGACAGCTTCTTAAATTACTAAAGCCCTATAGTTCTGTAGTAAAATCACCTACTACAGAACTATAGGGCTTT
TTGAATACGCAAAAACATCT

Product: transcription antitermination protein NusB

Products: NA

Alternate protein names: Protein nusB [H]

Number of amino acids: Translated: 139; Mature: 139

Protein sequence:

>139_residues
MRERAFQALFTMEMGGDFLLASQFAYDYDKEVADDKQPSELPVFLLNLVNGVMDHKAELDEVIKKNLKAGWSIERLTVVD
KTMLRLGLFEMTLFEETPDRVALNEIIEIAKKYSDDTSAKFINGLLSQFVSDESVAVTD

Sequences:

>Translated_139_residues
MRERAFQALFTMEMGGDFLLASQFAYDYDKEVADDKQPSELPVFLLNLVNGVMDHKAELDEVIKKNLKAGWSIERLTVVD
KTMLRLGLFEMTLFEETPDRVALNEIIEIAKKYSDDTSAKFINGLLSQFVSDESVAVTD
>Mature_139_residues
MRERAFQALFTMEMGGDFLLASQFAYDYDKEVADDKQPSELPVFLLNLVNGVMDHKAELDEVIKKNLKAGWSIERLTVVD
KTMLRLGLFEMTLFEETPDRVALNEIIEIAKKYSDDTSAKFINGLLSQFVSDESVAVTD

Specific function: Involved in the transcription termination process [H]

COG id: COG0781

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nusB family [H]

Homologues:

Organism=Escherichia coli, GI1786618, Length=126, Percent_Identity=30.1587301587302, Blast_Score=59, Evalue=8e-11,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011605
- InterPro:   IPR006027 [H]

Pfam domain/function: PF01029 NusB [H]

EC number: NA

Molecular weight: Translated: 15776; Mature: 15776

Theoretical pI: Translated: 4.22; Mature: 4.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
4.3 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
4.3 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRERAFQALFTMEMGGDFLLASQFAYDYDKEVADDKQPSELPVFLLNLVNGVMDHKAELD
CCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EVIKKNLKAGWSIERLTVVDKTMLRLGLFEMTLFEETPDRVALNEIIEIAKKYSDDTSAK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHH
FINGLLSQFVSDESVAVTD
HHHHHHHHHHCCCCEECCC
>Mature Secondary Structure
MRERAFQALFTMEMGGDFLLASQFAYDYDKEVADDKQPSELPVFLLNLVNGVMDHKAELD
CCHHHHHHHHHHHCCCCEEHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHH
EVIKKNLKAGWSIERLTVVDKTMLRLGLFEMTLFEETPDRVALNEIIEIAKKYSDDTSAK
HHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHH
FINGLLSQFVSDESVAVTD
HHHHHHHHHHCCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA