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The map label for this gene is dinB [H]

Identifier: 195977472

GI number: 195977472

Start: 347357

End: 348460

Strand: Direct

Name: dinB [H]

Synonym: Sez_0327

Alternate gene names: 195977472

Gene position: 347357-348460 (Clockwise)

Preceding gene: 195977470

Following gene: 195977477

Centisome position: 17.16

GC content: 45.02

Gene sequence:

>1104_bases
ATGCTTATTTTTCCGCTGATTAATGACACCTCACGCAAGATTATACACATTGACATGGATGCTTTTTTTGCTGCGGTGGA
AGAAAGAGATAATCCCGAATTAAAGGGCAAGCCGGTGGTGATCGGTAAGGACCCTAGAGAAAGTGGTGGACGGGGCGTTG
TATCAACCTGTAACTACGAGGCAAGAAGGTTTGGCATTCACTCTGCAATGAGCTCAAAGGAGGCCTATGAGCGTTGTCCT
CAGGCTATTTTTATCTCTGGAGATCATGCTAAGTACCGTAGGATTGGCTATGATATCAGAGAGATTTTCAGGCGTTATAC
GGATCAGGTGGAGCCTGTGTCTATTGATGAGGCTTATTTAGACGTGACAAGCAATAAGCTGGGCATCAAGTCTGCCTTAA
AGATTGCAAGGCTGATTCAGTATGATATTTGGAATAGCCTGCATTTGACCTGCTCTGCTGGTGTTTCTTATAACAAATTT
TTAGCCAAGCTAGCTAGTGATTTTGAAAAGCCACATGGTCTGACCCTAGTGCTTCCTGATGATGCTCTGCCATTCTTAGA
AAAGCTTCCGATTGAAAAGTTTCATGGCGTTGGTAAGCGAACAGTTGAAAGGCTGCATGAGATGGGGATTTACACTGGGC
AGGACCTCTTGCGTGTTCCTGAGATGACCCTGATTGATCAGTTTGGACGCTTTGGCTATGAGCTTTATCGAAAGGCCAGA
GGCATTAGCCATTCGCCAGTCAAGCCTAATCGTATTCGCAAATCCATCAGCAGTGAGCGAACCTATGCCAAGCTCCTCTA
TCAAGAGGCTGATATTAAGGCGGAGATTTCCAAAAATGCCAGCCGGGTCGCTGATTTGCTTGAGGCGAATAAAAAGCTAG
GAAGGATCATTGTTTTGAAGGTTCGGTATGCTGACTTTACGACCTTGACCAAGCGGGTTAGCCTACCTGAGTTAACAAGA
GATGCCAGGCTCATTGATCAGACGGCTAGAGAGCTTTTTGATAGCTTGGAGGAGCATAAGGAAGGAATCCGCTTATTGGG
AGTCACAATGACCGGATTAAAGGATAAAGTGACAGATATTTTGTTGGACCTATCATTCAACTAA

Upstream 100 bases:

>100_bases
CTTTTATATTACAGTTTGCTGAAACAGTTGAAAACTGTAATACAATGCGCTATCTTGGCTTTAAAGGCCTAAAGGAGTAT
AATGTAGGAGGGAGGGACCT

Downstream 100 bases:

>100_bases
CAAAAAAAGCAAGAGGACATTATCAATTGTCCTCTTGCTTTTTTAGTGGTTAAAAAAGGCTCTTTGTCAACCGTAGTGGG
CTGACAAAGAGCCATATTTG

Product: DNA polymerase IV

Products: NA

Alternate protein names: Pol IV [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPELKGKPVVIGKDPRESGGRGVVSTCNYEARRFGIHSAMSSKEAYERCP
QAIFISGDHAKYRRIGYDIREIFRRYTDQVEPVSIDEAYLDVTSNKLGIKSALKIARLIQYDIWNSLHLTCSAGVSYNKF
LAKLASDFEKPHGLTLVLPDDALPFLEKLPIEKFHGVGKRTVERLHEMGIYTGQDLLRVPEMTLIDQFGRFGYELYRKAR
GISHSPVKPNRIRKSISSERTYAKLLYQEADIKAEISKNASRVADLLEANKKLGRIIVLKVRYADFTTLTKRVSLPELTR
DARLIDQTARELFDSLEEHKEGIRLLGVTMTGLKDKVTDILLDLSFN

Sequences:

>Translated_367_residues
MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPELKGKPVVIGKDPRESGGRGVVSTCNYEARRFGIHSAMSSKEAYERCP
QAIFISGDHAKYRRIGYDIREIFRRYTDQVEPVSIDEAYLDVTSNKLGIKSALKIARLIQYDIWNSLHLTCSAGVSYNKF
LAKLASDFEKPHGLTLVLPDDALPFLEKLPIEKFHGVGKRTVERLHEMGIYTGQDLLRVPEMTLIDQFGRFGYELYRKAR
GISHSPVKPNRIRKSISSERTYAKLLYQEADIKAEISKNASRVADLLEANKKLGRIIVLKVRYADFTTLTKRVSLPELTR
DARLIDQTARELFDSLEEHKEGIRLLGVTMTGLKDKVTDILLDLSFN
>Mature_367_residues
MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPELKGKPVVIGKDPRESGGRGVVSTCNYEARRFGIHSAMSSKEAYERCP
QAIFISGDHAKYRRIGYDIREIFRRYTDQVEPVSIDEAYLDVTSNKLGIKSALKIARLIQYDIWNSLHLTCSAGVSYNKF
LAKLASDFEKPHGLTLVLPDDALPFLEKLPIEKFHGVGKRTVERLHEMGIYTGQDLLRVPEMTLIDQFGRFGYELYRKAR
GISHSPVKPNRIRKSISSERTYAKLLYQEADIKAEISKNASRVADLLEANKKLGRIIVLKVRYADFTTLTKRVSLPELTR
DARLIDQTARELFDSLEEHKEGIRLLGVTMTGLKDKVTDILLDLSFN

Specific function: Poorly processive, error-prone DNA polymerase involved in untargeted mutagenesis. Copies undamaged DNA at stalled replication forks, which arise in vivo from mismatched or misaligned primer ends. These misaligned primers can be extended by polIV. Exhibits

COG id: COG0389

COG function: function code L; Nucleotidyltransferase/DNA polymerase involved in DNA repair

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 umuC domain [H]

Homologues:

Organism=Homo sapiens, GI154350220, Length=299, Percent_Identity=29.7658862876254, Blast_Score=134, Evalue=2e-31,
Organism=Homo sapiens, GI84043967, Length=405, Percent_Identity=25.679012345679, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI7706681, Length=406, Percent_Identity=25.615763546798, Blast_Score=131, Evalue=9e-31,
Organism=Homo sapiens, GI5729982, Length=321, Percent_Identity=28.3489096573209, Blast_Score=107, Evalue=2e-23,
Organism=Homo sapiens, GI7705344, Length=113, Percent_Identity=46.0176991150442, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1786425, Length=350, Percent_Identity=41.4285714285714, Blast_Score=240, Evalue=9e-65,
Organism=Escherichia coli, GI1787432, Length=355, Percent_Identity=24.5070422535211, Blast_Score=86, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI193205700, Length=411, Percent_Identity=28.4671532846715, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17537959, Length=377, Percent_Identity=26.790450928382, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI115534089, Length=324, Percent_Identity=28.7037037037037, Blast_Score=95, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI193205702, Length=225, Percent_Identity=32, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19923006, Length=411, Percent_Identity=23.3576642335766, Blast_Score=116, Evalue=2e-26,
Organism=Drosophila melanogaster, GI21355641, Length=291, Percent_Identity=29.2096219931272, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24644984, Length=291, Percent_Identity=29.2096219931272, Blast_Score=109, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24668444, Length=126, Percent_Identity=34.1269841269841, Blast_Score=82, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017962
- InterPro:   IPR017961
- InterPro:   IPR001126
- InterPro:   IPR017963
- InterPro:   IPR022880 [H]

Pfam domain/function: PF00817 IMS [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 41780; Mature: 41780

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: PS50173 UMUC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPELKGKPVVIGKDPRESGGRGVVSTCNYE
CEEEEEECCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCH
ARRFGIHSAMSSKEAYERCPQAIFISGDHAKYRRIGYDIREIFRRYTDQVEPVSIDEAYL
HHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHH
DVTSNKLGIKSALKIARLIQYDIWNSLHLTCSAGVSYNKFLAKLASDFEKPHGLTLVLPD
EECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECC
DALPFLEKLPIEKFHGVGKRTVERLHEMGIYTGQDLLRVPEMTLIDQFGRFGYELYRKAR
CCCHHHHHCCHHHHHCCCHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHC
GISHSPVKPNRIRKSISSERTYAKLLYQEADIKAEISKNASRVADLLEANKKLGRIIVLK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHCCEEEEEE
VRYADFTTLTKRVSLPELTRDARLIDQTARELFDSLEEHKEGIRLLGVTMTGLKDKVTDI
EECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHCHHHHHHHH
LLDLSFN
HHEECCC
>Mature Secondary Structure
MLIFPLINDTSRKIIHIDMDAFFAAVEERDNPELKGKPVVIGKDPRESGGRGVVSTCNYE
CEEEEEECCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCEEEECCCCCCCCCCEEEECCCH
ARRFGIHSAMSSKEAYERCPQAIFISGDHAKYRRIGYDIREIFRRYTDQVEPVSIDEAYL
HHHHHHHHHHHHHHHHHHCCCEEEEECCCHHHHHHCHHHHHHHHHHHCCCCCCCCCHHHH
DVTSNKLGIKSALKIARLIQYDIWNSLHLTCSAGVSYNKFLAKLASDFEKPHGLTLVLPD
EECCCCCCHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHCCCCCEEEEECC
DALPFLEKLPIEKFHGVGKRTVERLHEMGIYTGQDLLRVPEMTLIDQFGRFGYELYRKAR
CCCHHHHHCCHHHHHCCCHHHHHHHHHCCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHC
GISHSPVKPNRIRKSISSERTYAKLLYQEADIKAEISKNASRVADLLEANKKLGRIIVLK
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHCCEEEEEE
VRYADFTTLTKRVSLPELTRDARLIDQTARELFDSLEEHKEGIRLLGVTMTGLKDKVTDI
EECCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEEHHCHHHHHHHH
LLDLSFN
HHEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12122206; 12799345 [H]