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The map label for this gene is pgk [H]

Identifier: 195977419

GI number: 195977419

Start: 294516

End: 295712

Strand: Direct

Name: pgk [H]

Synonym: Sez_0272

Alternate gene names: 195977419

Gene position: 294516-295712 (Clockwise)

Preceding gene: 195977418

Following gene: 195977420

Centisome position: 14.55

GC content: 42.86

Gene sequence:

>1197_bases
ATGGCTAAATTGACTGTTAAAGACCTTGACCTAAAGGGTAAAAAAGTGCTCGTTCGCGTTGACTTTAATGTCCCTTTGAA
AAATGGTGTTATTACAAACGATAACCGTATTTCTGCAGCACTTCCAACAATCAAATACATTATTGAGCATGGTGGTCGCG
CTATCCTTTTCTCACACCTAGGACGTGTAAAGGAAGAGGCAGACAAGGAAGGCAAATCATTGGCGCCAGTAGCGAAAAAC
TTGGCTGAAAAGCTTGGTCAAGAGGTTATTTTCCCTGGCAGCACACGTGGGGCAGAACTTGAAGCTGCTATTGATGCTCT
TGAGGATGGACAGGTTCTTTTGGTTGAAAACACTCGCTTTGAAGATATTGACGGTAAAAAGGAATCTAAAAACGATCCAG
AGCTTGGTAAATACTGGGCATCACTAGGAGAAGGGATTTTTGTTAATGATGCCTTTGGTACAGCTCACCGCACTCATGCA
TCAAACGTTGGTATCTCTGCAAACGTTGAAAAAGCAGTGGCTGGCTTCCTTCTTGAAAATGAAATTGCCTATATCAAAGA
AGCTGTTGAAGCTCCAGAGCGTCCATTTGTCGCTATCCTTGGTGGCTCAAAAGTTTCTGACAAGATTGGTGTGATTGAAA
ATCTCCTTGAAAAAGCTGATAAGGTGCTTATCGGTGGTGGTATGACCTATACCTTCTACAAAGCTCAAGGCATTGAAATT
GGAAACTCACTTTGTGAAGAGGACAAGCTTGATGTGGCAAAATCACTTCTTGAAAAATCAAACGGCAAGCTTATTTTGCC
AGTTGATTCTAAGGAAGCAAACGCTTTTGCCGATTACACTGAAGTGAAGGTAACTGAGGGTGAAGCAGTTGATGCAGGCT
TCCTTGGACTTGACATTGGTCCAAAATCTATCGCTAAATTTGATGAAGCTCTTACAGGTGCTAAAACAGTTGTTTGGAAT
GGTCCTATGGGTGTCTTTGAAAATCCTGATTTCCAAGAAGGTACAATTGGTGTGATGGACGCTATTGTGAAGCAGCCAGG
TGTTAAGTCAATCATCGGTGGAGGTGATTCAGCAGCGGCAGCCATCAACCTTGGACGTGCAGATAAATTCTCATGGATTA
GTACAGGTGGAGGTGCTTCAATGGAGCTCCTTGAAGGTAAGGAGCTTCCAGGCTTAGCAGCTCTTACAGAAAAATAA

Upstream 100 bases:

>100_bases
AAAATTGTGAAATAGTTAATTTATTGTTTTCAGATTTTATCCTTATCAAAAGAATTGTGTTATAATAGAGTGTACTAAAA
AATATTAAGGAGTCTATGAA

Downstream 100 bases:

>100_bases
GCTGATACCATTTCAGTGCTGTCAGTCGTAGGTTGCTATATGCCTTGTTAAGGGCTAAGACAGCTGAAGACTCACAAAGG
TGGCTTATGTTGCTGGCGGT

Product: phosphoglycerate kinase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MAKLTVKDLDLKGKKVLVRVDFNVPLKNGVITNDNRISAALPTIKYIIEHGGRAILFSHLGRVKEEADKEGKSLAPVAKN
LAEKLGQEVIFPGSTRGAELEAAIDALEDGQVLLVENTRFEDIDGKKESKNDPELGKYWASLGEGIFVNDAFGTAHRTHA
SNVGISANVEKAVAGFLLENEIAYIKEAVEAPERPFVAILGGSKVSDKIGVIENLLEKADKVLIGGGMTYTFYKAQGIEI
GNSLCEEDKLDVAKSLLEKSNGKLILPVDSKEANAFADYTEVKVTEGEAVDAGFLGLDIGPKSIAKFDEALTGAKTVVWN
GPMGVFENPDFQEGTIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKELPGLAALTEK

Sequences:

>Translated_398_residues
MAKLTVKDLDLKGKKVLVRVDFNVPLKNGVITNDNRISAALPTIKYIIEHGGRAILFSHLGRVKEEADKEGKSLAPVAKN
LAEKLGQEVIFPGSTRGAELEAAIDALEDGQVLLVENTRFEDIDGKKESKNDPELGKYWASLGEGIFVNDAFGTAHRTHA
SNVGISANVEKAVAGFLLENEIAYIKEAVEAPERPFVAILGGSKVSDKIGVIENLLEKADKVLIGGGMTYTFYKAQGIEI
GNSLCEEDKLDVAKSLLEKSNGKLILPVDSKEANAFADYTEVKVTEGEAVDAGFLGLDIGPKSIAKFDEALTGAKTVVWN
GPMGVFENPDFQEGTIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKELPGLAALTEK
>Mature_397_residues
AKLTVKDLDLKGKKVLVRVDFNVPLKNGVITNDNRISAALPTIKYIIEHGGRAILFSHLGRVKEEADKEGKSLAPVAKNL
AEKLGQEVIFPGSTRGAELEAAIDALEDGQVLLVENTRFEDIDGKKESKNDPELGKYWASLGEGIFVNDAFGTAHRTHAS
NVGISANVEKAVAGFLLENEIAYIKEAVEAPERPFVAILGGSKVSDKIGVIENLLEKADKVLIGGGMTYTFYKAQGIEIG
NSLCEEDKLDVAKSLLEKSNGKLILPVDSKEANAFADYTEVKVTEGEAVDAGFLGLDIGPKSIAKFDEALTGAKTVVWNG
PMGVFENPDFQEGTIGVMDAIVKQPGVKSIIGGGDSAAAAINLGRADKFSWISTGGGASMELLEGKELPGLAALTEK

Specific function: Second phase of glycolysis; second step. [C]

COG id: COG0126

COG function: function code G; 3-phosphoglycerate kinase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the phosphoglycerate kinase family [H]

Homologues:

Organism=Homo sapiens, GI4505763, Length=412, Percent_Identity=45.873786407767, Blast_Score=332, Evalue=3e-91,
Organism=Homo sapiens, GI31543397, Length=412, Percent_Identity=44.4174757281553, Blast_Score=322, Evalue=3e-88,
Organism=Escherichia coli, GI1789294, Length=400, Percent_Identity=42.75, Blast_Score=281, Evalue=4e-77,
Organism=Caenorhabditis elegans, GI17508823, Length=414, Percent_Identity=47.3429951690821, Blast_Score=337, Evalue=4e-93,
Organism=Saccharomyces cerevisiae, GI6319857, Length=414, Percent_Identity=49.0338164251208, Blast_Score=347, Evalue=3e-96,
Organism=Drosophila melanogaster, GI17136394, Length=413, Percent_Identity=45.5205811138014, Blast_Score=311, Evalue=7e-85,
Organism=Drosophila melanogaster, GI24581203, Length=417, Percent_Identity=44.3645083932854, Blast_Score=298, Evalue=4e-81,

Paralogues:

None

Copy number: 2990 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1460 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001576
- InterPro:   IPR015901
- InterPro:   IPR015911
- InterPro:   IPR015824 [H]

Pfam domain/function: PF00162 PGK [H]

EC number: =2.7.2.3 [H]

Molecular weight: Translated: 42241; Mature: 42109

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00111 PGLYCERATE_KINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKLTVKDLDLKGKKVLVRVDFNVPLKNGVITNDNRISAALPTIKYIIEHGGRAILFSHL
CCEEEEECCCCCCCEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEHHHHH
GRVKEEADKEGKSLAPVAKNLAEKLGQEVIFPGSTRGAELEAAIDALEDGQVLLVENTRF
HHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCC
EDIDGKKESKNDPELGKYWASLGEGIFVNDAFGTAHRTHASNVGISANVEKAVAGFLLEN
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHCCCCCCCCCCCHHHHHHHHHHHH
EIAYIKEAVEAPERPFVAILGGSKVSDKIGVIENLLEKADKVLIGGGMTYTFYKAQGIEI
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCEEEECCCEEEEEEEECCCHH
GNSLCEEDKLDVAKSLLEKSNGKLILPVDSKEANAFADYTEVKVTEGEAVDAGFLGLDIG
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEECCCEECCCEEEECCC
PKSIAKFDEALTGAKTVVWNGPMGVFENPDFQEGTIGVMDAIVKQPGVKSIIGGGDSAAA
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCEE
AINLGRADKFSWISTGGGASMELLEGKELPGLAALTEK
EEECCCCCCCEEEECCCCCEEEEECCCCCCCCHHCCCC
>Mature Secondary Structure 
AKLTVKDLDLKGKKVLVRVDFNVPLKNGVITNDNRISAALPTIKYIIEHGGRAILFSHL
CEEEEECCCCCCCEEEEEEECCCCCCCCEEECCCCCHHHHHHHHHHHHCCCCEEHHHHH
GRVKEEADKEGKSLAPVAKNLAEKLGQEVIFPGSTRGAELEAAIDALEDGQVLLVENTRF
HHHHHHHHHCCCCHHHHHHHHHHHCCCEEECCCCCCCCHHHHHHHHCCCCCEEEEECCCC
EDIDGKKESKNDPELGKYWASLGEGIFVNDAFGTAHRTHASNVGISANVEKAVAGFLLEN
CCCCCCCCCCCCCHHHHHHHHHCCCEEEECCCCCCHHCCCCCCCCCCCHHHHHHHHHHHH
EIAYIKEAVEAPERPFVAILGGSKVSDKIGVIENLLEKADKVLIGGGMTYTFYKAQGIEI
HHHHHHHHHCCCCCCEEEEECCCCCCHHHHHHHHHHHHHCEEEECCCEEEEEEEECCCHH
GNSLCEEDKLDVAKSLLEKSNGKLILPVDSKEANAFADYTEVKVTEGEAVDAGFLGLDIG
HHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCCCCCCCCEEEEEECCCEECCCEEEECCC
PKSIAKFDEALTGAKTVVWNGPMGVFENPDFQEGTIGVMDAIVKQPGVKSIIGGGDSAAA
HHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCHHHHHHHHCCCCCHHHHCCCCCCEE
AINLGRADKFSWISTGGGASMELLEGKELPGLAALTEK
EEECCCCCCCEEEECCCCCEEEEECCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA