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The map label for this gene is radA [H]

Identifier: 195977371

GI number: 195977371

Start: 246279

End: 247640

Strand: Direct

Name: radA [H]

Synonym: Sez_0224

Alternate gene names: 195977371

Gene position: 246279-247640 (Clockwise)

Preceding gene: 195977370

Following gene: 195977372

Centisome position: 12.17

GC content: 48.38

Gene sequence:

>1362_bases
ATGGCTAAGAAAAAGGCAAGGTTTATCTGTGGGGAATGTGGCTATCAGTCACCCAAATATCTGGGGCGCTGCCCTAATTG
CTCTGCTTGGTCTTCCTTTGTAGAGGAGACAGAGGTCAAGGACGTCAAAAATGCCCGTATTAGCCTGACTGGAGAAAAGA
GTAAGCCCATCAAGCTAAAGCATGTCAATGGCGTTCGCTACCAGCGTATCCAGACTGAGATGAATGAGTTCAATCGTGTG
CTGGGCGGAGGTGTTGTACCGGGGAGCTTGGTTTTGATTGGTGGTGATCCTGGTATTGGTAAGTCAACGCTGCTCTTGCA
GGTTTCTATCCAGCTTGCGGATAAGGGAACTGTTTTATATGTCTCTGGAGAGGAATCGGCAGAGCAGATCAAGATGCGCA
GTGATCGTTTGGGACAGACCGACAATGATTTTTACCTCTATGCGGAGACCAATATGCAGGCTATTCGAGCAGAGATTGAA
GCCATTCAGCCGGATTTTTTGATTATTGATTCTATTCAGACGATCATGAGTCCGGAGATTTCGGGTGTTCAGGGCTCGGT
CAGTCAGGTGAGAGAGGTCACTGCTGAGCTGCTCCAGCTAGCTAAGTCTAATCATATTGCAACCTTTATTGTAGGACATG
TCACCAAGGAAGGCACCCTAGCAGGGCCTCGCATGCTGGAGCATATGGTTGATACGGTCCTTTATTTTGAGGGCGAGAGG
CATCATACCTTTCGTATCTTGCGAGCGGTTAAAAATCGTTTTGGCTCAACCAATGAGATTGGTATTTTTGACATGCAATC
AGGTGGGCTAGTGGAGGTTCTCAATCCCAGTCAGGTGTTCTTAGAGGAACGATTAGATGGGGCAACAGGCTCTGCTGTGG
TTGTCACCATGGAGGGGAGCAGACCGATTTTGGCAGAGGTGCAGGCCTTAGTCACACCGACTGTTTTTGGCAATGCTAGA
CGAACCACTACTGGATTAGACTTTAATCGTGTTAGCCTGATTATGGCTGTCCTAGAAAAGCGCTGCGGCCTGCTGCTACA
AAATCAAGATGCCTACCTCAAATCAGCAGGAGGTGTGAAGCTAGATGAGCCTGCTATTGATTTGGCAGTTGCTGTGGCTA
TTGCTTCTAGCTACAAGGAAAAACCAACCAACCCTCAGGAGGCCTTCTTAGGAGAGATTGGCTTGACGGGCGAGATTCGG
CGGGTCACTCGTATTGAGCAGCGGATCAATGAGGCTGCAAAGCTTGGCTTTACCAAGCTTTACGCTCCTAAAAGCTCATT
GCAGGGCATTGACATACCAAGTGGGATTGAGGTTGTTGGTGTAATGACAGTAGGAGAGGTCTTAAAGGCAGTCTTTGCTT
AG

Upstream 100 bases:

>100_bases
AGCGGACAGGTGGCTTTGGTTCGACAGGAAGATAGGGTGTCAGTGGGCTGTGTCAGCAGTTGCAGCCTATTAGTGTTTAG
ATTCAAGGAGGGAATGTGCT

Downstream 100 bases:

>100_bases
GCTTGCTGCTTTTGATGTGAGGGTTAGGACAGTTATTTCTAGCCCTTTTTGGTTTGCCAGTAGCTATTGGTAGCTTTCTT
ATGGTGTCTCATAAGGCTAA

Product: DNA repair protein RadA

Products: NA

Alternate protein names: DNA repair protein sms homolog [H]

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MAKKKARFICGECGYQSPKYLGRCPNCSAWSSFVEETEVKDVKNARISLTGEKSKPIKLKHVNGVRYQRIQTEMNEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSIQLADKGTVLYVSGEESAEQIKMRSDRLGQTDNDFYLYAETNMQAIRAEIE
AIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELLQLAKSNHIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFDMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQALVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKLYAPKSSLQGIDIPSGIEVVGVMTVGEVLKAVFA

Sequences:

>Translated_453_residues
MAKKKARFICGECGYQSPKYLGRCPNCSAWSSFVEETEVKDVKNARISLTGEKSKPIKLKHVNGVRYQRIQTEMNEFNRV
LGGGVVPGSLVLIGGDPGIGKSTLLLQVSIQLADKGTVLYVSGEESAEQIKMRSDRLGQTDNDFYLYAETNMQAIRAEIE
AIQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELLQLAKSNHIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
HHTFRILRAVKNRFGSTNEIGIFDMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQALVTPTVFGNAR
RTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQEAFLGEIGLTGEIR
RVTRIEQRINEAAKLGFTKLYAPKSSLQGIDIPSGIEVVGVMTVGEVLKAVFA
>Mature_452_residues
AKKKARFICGECGYQSPKYLGRCPNCSAWSSFVEETEVKDVKNARISLTGEKSKPIKLKHVNGVRYQRIQTEMNEFNRVL
GGGVVPGSLVLIGGDPGIGKSTLLLQVSIQLADKGTVLYVSGEESAEQIKMRSDRLGQTDNDFYLYAETNMQAIRAEIEA
IQPDFLIIDSIQTIMSPEISGVQGSVSQVREVTAELLQLAKSNHIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGERH
HTFRILRAVKNRFGSTNEIGIFDMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGSRPILAEVQALVTPTVFGNARR
TTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVKLDEPAIDLAVAVAIASSYKEKPTNPQEAFLGEIGLTGEIRR
VTRIEQRINEAAKLGFTKLYAPKSSLQGIDIPSGIEVVGVMTVGEVLKAVFA

Specific function: May play a role in the repair of endogenous alkylation damage [H]

COG id: COG1066

COG function: function code O; Predicted ATP-dependent serine protease

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the recA family. RadA subfamily [H]

Homologues:

Organism=Escherichia coli, GI1790850, Length=459, Percent_Identity=41.6122004357298, Blast_Score=366, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR014774
- InterPro:   IPR004504
- InterPro:   IPR008269
- InterPro:   IPR020568 [H]

Pfam domain/function: PF06745 KaiC; PF05362 Lon_C [H]

EC number: NA

Molecular weight: Translated: 49334; Mature: 49203

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKKKARFICGECGYQSPKYLGRCPNCSAWSSFVEETEVKDVKNARISLTGEKSKPIKLK
CCCCCCCEEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEE
HVNGVRYQRIQTEMNEFNRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSIQLADKGTVLY
ECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEEEEECCCEEEE
VSGEESAEQIKMRSDRLGQTDNDFYLYAETNMQAIRAEIEAIQPDFLIIDSIQTIMSPEI
EECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECHHHHHCCCC
SGVQGSVSQVREVTAELLQLAKSNHIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHEECCCC
HHTFRILRAVKNRFGSTNEIGIFDMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGS
HHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHHCCCCCCCEEEEEECCC
RPILAEVQALVTPTVFGNARRTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVK
CCHHHHHHHHHCCHHCCCCCCEECCCCHHHHHHHHHHHHHHCCEEEECCCHHHHCCCCCE
LDEPAIDLAVAVAIASSYKEKPTNPQEAFLGEIGLTGEIRRVTRIEQRINEAAKLGFTKL
ECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEE
YAPKSSLQGIDIPSGIEVVGVMTVGEVLKAVFA
ECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHC
>Mature Secondary Structure 
AKKKARFICGECGYQSPKYLGRCPNCSAWSSFVEETEVKDVKNARISLTGEKSKPIKLK
CCCCCCEEECCCCCCCCHHHCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCCEEEE
HVNGVRYQRIQTEMNEFNRVLGGGVVPGSLVLIGGDPGIGKSTLLLQVSIQLADKGTVLY
ECCCCHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCCCCCCEEEEEEEEEEECCCEEEE
VSGEESAEQIKMRSDRLGQTDNDFYLYAETNMQAIRAEIEAIQPDFLIIDSIQTIMSPEI
EECCCCHHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHHHCCCCEEEEECHHHHHCCCC
SGVQGSVSQVREVTAELLQLAKSNHIATFIVGHVTKEGTLAGPRMLEHMVDTVLYFEGER
CCCCCHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCCCCHHHHHHHHHHHHEECCCC
HHTFRILRAVKNRFGSTNEIGIFDMQSGGLVEVLNPSQVFLEERLDGATGSAVVVTMEGS
HHHHHHHHHHHHHCCCCCCEEEEEECCCCEEEECCCHHHHHHHHCCCCCCCEEEEEECCC
RPILAEVQALVTPTVFGNARRTTTGLDFNRVSLIMAVLEKRCGLLLQNQDAYLKSAGGVK
CCHHHHHHHHHCCHHCCCCCCEECCCCHHHHHHHHHHHHHHCCEEEECCCHHHHCCCCCE
LDEPAIDLAVAVAIASSYKEKPTNPQEAFLGEIGLTGEIRRVTRIEQRINEAAKLGFTKL
ECCCHHHHHHHHHHHHHHCCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCEE
YAPKSSLQGIDIPSGIEVVGVMTVGEVLKAVFA
ECCCCCCCCCCCCCCCEEEEHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11296296 [H]