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The map label for this gene is tgt [H]

Identifier: 195977336

GI number: 195977336

Start: 207978

End: 209186

Strand: Direct

Name: tgt [H]

Synonym: Sez_0189

Alternate gene names: 195977336

Gene position: 207978-209186 (Clockwise)

Preceding gene: 195977333

Following gene: 195977337

Centisome position: 10.27

GC content: 47.81

Gene sequence:

>1209_bases
GTGCTTTGTAAGTGGTATGCAAAACGCTCAAGACTGTTTATCTTGATAGCAAGGAAAAAAGAAACAATGACTAATTATCC
TATTACTTATCGTTTGATTAAAAAGGAAAAGCACACAGGTGCTCGTCTCGGTGAGATTATCACCCCGCATGGGACCTTTC
CGACTCCCATGTTTATGCCGGTAGGGACACAGGCGACGGTTAAGACACAGTCTCCTGAGGAGCTCAAGGAGATTGGGTCA
GGTATTATTTTATCAAACACCTATCACCTATGGCTAAGACCAGGAGATGAGCTGATTGCGCGTGCAGGTGGTCTCCACAA
ATTTATGAACTGGGATCAGGCAATCCTGACAGACTCAGGCGGCTTTCAGGTTTACTCCTTGGCAGACTCCAGAAATATCA
CTGAGGAGGGGGTGACCTTTAAAAATCACCTGAACGGCTCAAAGATGTTTTTATCACCAGAGAAGGCTATCTCTATTCAA
AATAACCTAGGCTCTGACATTATGATGAGCTTTGATGAGTGCCCGCAATTTTACCAGCCCTATGATTATGTGAAAAAGTC
AATTGAGCGCACCAGCCGCTGGGCAGAGCGTGGTCTGAAGGCCCATCGTCGTCCTCATGACCAAGGCCTCTTTGGTATTG
TGCAAGGGGCAGGCTTTGAGGATCTGCGTCGTCAGTCTGCTGCGGACCTGGTGGGCATGGATTTTCCGGGCTATTCTATC
GGTGGCTTGGCAGTTGGGGAGAGCCATGCTGAGATGAATGCTGTCCTTGATTTTACAACGCCACTTTTACCTGAAAATAA
GCCACGCTACCTGATGGGGGTAGGTGCACCTGATAGCCTGATTGATGGGGTGATTCGTGGGGTTGACATGTTTGACTGCG
TCCTGCCAACCCGTATCGCTCGCAATGGCACCTGCATGACCAGCGAGGGCCGTCTCGTTGTGAAAAATGCTAAGTTTGCA
GAGGACTTTACCCCACTTGACCATCATTGTGACTGCTACACCTGTCAGCATTACACACGTGCTTACCTACGTCATCTCCT
AAAGGCTGATGAGACCTTTGGCATGCGCTTGACCAGTTACCATAACCTATACTTCTTAGTAAACCTCATGAAGCAGGTGC
GTCAAGCTATCTTGGACGATAACCTTTTAGAGTTTCGTCAGGATTTCCTTGAGCGCTATGGCTACAACAGCTCCAGTCGT
AATTTTTAA

Upstream 100 bases:

>100_bases
CCGTCATTGTTCAGAGCTATTGTCTCTTTGAGGGCATGACAAGTCAGGGATTTTTTGATAAAATGATAGGGCTCTAAACA
AGCCCATACCGCTTGGCTAA

Downstream 100 bases:

>100_bases
GCTGATAGCCGCCTAAGAGCGGTAGCTTTCCACAATAACAGATAATAGCAGCAAGGGTCTGGGCTTTCGCCTAGGCCTTT
TAAGCCTGCCTATCAAAAGA

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MLCKWYAKRSRLFILIARKKETMTNYPITYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKEIGS
GIILSNTYHLWLRPGDELIARAGGLHKFMNWDQAILTDSGGFQVYSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQ
NNLGSDIMMSFDECPQFYQPYDYVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVGMDFPGYSI
GGLAVGESHAEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFA
EDFTPLDHHCDCYTCQHYTRAYLRHLLKADETFGMRLTSYHNLYFLVNLMKQVRQAILDDNLLEFRQDFLERYGYNSSSR
NF

Sequences:

>Translated_402_residues
MLCKWYAKRSRLFILIARKKETMTNYPITYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKEIGS
GIILSNTYHLWLRPGDELIARAGGLHKFMNWDQAILTDSGGFQVYSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQ
NNLGSDIMMSFDECPQFYQPYDYVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVGMDFPGYSI
GGLAVGESHAEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFA
EDFTPLDHHCDCYTCQHYTRAYLRHLLKADETFGMRLTSYHNLYFLVNLMKQVRQAILDDNLLEFRQDFLERYGYNSSSR
NF
>Mature_402_residues
MLCKWYAKRSRLFILIARKKETMTNYPITYRLIKKEKHTGARLGEIITPHGTFPTPMFMPVGTQATVKTQSPEELKEIGS
GIILSNTYHLWLRPGDELIARAGGLHKFMNWDQAILTDSGGFQVYSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQ
NNLGSDIMMSFDECPQFYQPYDYVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVGMDFPGYSI
GGLAVGESHAEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIARNGTCMTSEGRLVVKNAKFA
EDFTPLDHHCDCYTCQHYTRAYLRHLLKADETFGMRLTSYHNLYFLVNLMKQVRQAILDDNLLEFRQDFLERYGYNSSSR
NF

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=374, Percent_Identity=44.1176470588235, Blast_Score=338, Evalue=6e-93,
Organism=Escherichia coli, GI1786607, Length=355, Percent_Identity=51.2676056338028, Blast_Score=380, Evalue=1e-107,
Organism=Caenorhabditis elegans, GI17544680, Length=354, Percent_Identity=45.1977401129944, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI25146502, Length=207, Percent_Identity=30.4347826086957, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI19920510, Length=367, Percent_Identity=44.141689373297, Blast_Score=324, Evalue=7e-89,
Organism=Drosophila melanogaster, GI21355311, Length=222, Percent_Identity=26.5765765765766, Blast_Score=89, Evalue=4e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 45742; Mature: 45742

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLCKWYAKRSRLFILIARKKETMTNYPITYRLIKKEKHTGARLGEIITPHGTFPTPMFMP
CCCHHHCCCCCEEEEEEECCCCCCCCCEEEEEEEHHCCCCHHHCCEECCCCCCCCCEEEE
VGTQATVKTQSPEELKEIGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQAILTDSG
CCCCEEEECCCHHHHHHHCCCEEEECEEEEEECCCHHHHHHHCCHHHHHCCCCEEEECCC
GFQVYSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQP
CEEEEEECCCCCCCCCCCCHHHCCCCCEEEECHHHHEEECCCCCHHHHHHHHHCHHHCCC
YDYVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVGMDFPGYSI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHEEECCCCCCCC
GGLAVGESHAEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIA
CCEEECCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHH
RNGTCMTSEGRLVVKNAKFAEDFTPLDHHCDCYTCQHYTRAYLRHLLKADETFGMRLTSY
CCCCEEECCCEEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHH
HNLYFLVNLMKQVRQAILDDNLLEFRQDFLERYGYNSSSRNF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MLCKWYAKRSRLFILIARKKETMTNYPITYRLIKKEKHTGARLGEIITPHGTFPTPMFMP
CCCHHHCCCCCEEEEEEECCCCCCCCCEEEEEEEHHCCCCHHHCCEECCCCCCCCCEEEE
VGTQATVKTQSPEELKEIGSGIILSNTYHLWLRPGDELIARAGGLHKFMNWDQAILTDSG
CCCCEEEECCCHHHHHHHCCCEEEECEEEEEECCCHHHHHHHCCHHHHHCCCCEEEECCC
GFQVYSLADSRNITEEGVTFKNHLNGSKMFLSPEKAISIQNNLGSDIMMSFDECPQFYQP
CEEEEEECCCCCCCCCCCCHHHCCCCCEEEECHHHHEEECCCCCHHHHHHHHHCHHHCCC
YDYVKKSIERTSRWAERGLKAHRRPHDQGLFGIVQGAGFEDLRRQSAADLVGMDFPGYSI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEECCCCHHHHHHHHHHHEEECCCCCCCC
GGLAVGESHAEMNAVLDFTTPLLPENKPRYLMGVGAPDSLIDGVIRGVDMFDCVLPTRIA
CCEEECCCHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHCCHHHHHHHHHHHH
RNGTCMTSEGRLVVKNAKFAEDFTPLDHHCDCYTCQHYTRAYLRHLLKADETFGMRLTSY
CCCCEEECCCEEEEECCCHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCEEEHHH
HNLYFLVNLMKQVRQAILDDNLLEFRQDFLERYGYNSSSRNF
HHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA