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The map label for this gene is opuAB [H]

Identifier: 195977325

GI number: 195977325

Start: 192328

End: 194058

Strand: Direct

Name: opuAB [H]

Synonym: Sez_0178

Alternate gene names: 195977325

Gene position: 192328-194058 (Clockwise)

Preceding gene: 195977324

Following gene: 195977327

Centisome position: 9.5

GC content: 46.16

Gene sequence:

>1731_bases
TTGGAAAACCTATTACAAACAACTCTACCAGTGGCACAGCTGGTTGAGCAACTAACTGAGTGGCTGACAAAAACCTTTTC
AGGCTTCTTTGATCTGCTGCAGCTTGTGGGCAATACTTTGATGGATTGGATCACGCAGACCCTTTTGTTTATCAATCCGT
TACTGTTTATGCTATTGGTTACCTGTGCGATGTTTTTCTTGGCAAGAAAAAAATGGCCTTTGCCGATCTTTACCCTCCTG
GGTCTGTTATTTGTCTATAACCAAGGTCTGTGGGCAGAGTTGATCAATACCTTTACGCTGGTTTTGGTAGCAAGCCTCAT
CTCTGTCTTGCTTGGTATTCCCTTGGGCATTTGGATGGCTAAAAGTAAGACTGTTCACCAGGTGATTAATCCAATGCTGG
ACCTTATGCAGACCATGCCAGCCTTTGTTTATTTGATTCCGGCTGTAGCCTTTTTTGGCATTGGTATGGTCCCTGGTGTC
TTTGCATCGGTTATCTTTGCCTTGCCGCCGACTGTTCGCTTTACCAATTTAGCGATTCGTCATATTCCGACAGAATTGGT
TGAGGCTTCAGATGCCTTTGGCTCAACCCCTAAACAAAAATTACTGAAGGTCGAATTACCCTTGGCAAAGCATACGATTA
TGGCAGGTGTTAATCAGACCATGATGTTGGCTCTATCAATGGTTGTGACAGGCTCAATGATTGGTGCTCCAGGTCTTGGA
CGTGAGGTCCTATCGGCCTTGCAGCATGCTGATATTGGGCGTGGTTTTGTTAGTGGCTTGGCTCTTGTTATCTTGGCGAT
TATTTTGGATCGTATGACCCAGCATTTTAACGGTAAGCCACAAGAAAGGACGCAGACTGGCAAAACCAAGAAATGGCTTG
GTCTAGCTGCTTTAGCTGTCTTTTTGTTGTCAGCTTTGGGACGTGGCTTTGCTGCGATGTCATCAAGCTCATCAGACAAG
GGGCAAAAGGTGACCATTGCTTATGTGCAGTGGGATTCTGAGGTGGCTTCTACTCATGTTATCGCTCAGGTCTTAAGAGA
TGAGGGCTATCAGGTCACCTTGACGCCACTTGATAATGCTGTGATGTGGCAAACCATCGCAAATGGCGATGCAGACTTTT
CAACAAGTGCCTGGTTACCAGTGACCCACCAGCAGCAGTATCAAAAATATCAGGATAAGCTGGACAACCTTGGCCCTAAC
CTGAAGGGAACAAAGCTAGGACTTGCTGTGCCAGCCTATATGAGCGATGTGAACAGCATAGAGGAGCTTTCTGACCAAGC
CAATCAACAGATTATTGGGATTGAACCGGGAGCTGGTATTATGACTGCTGCTGATAAGACGCAAAGGGCCTATTCCAACT
TGGCAGATTGGGAGCTCATTGCTGCTTCGACAGGTGCTATGACCACCTCACTTGATCAAGCCATCAAGAAAAAAGAGCCG
ATCGTTGTGACGGCTTGGTCTCCTCATTGGATATTTGCTAAGTACGATTTGAAATACCTGGCAGATCCAAAGAAAACCTT
TGGTTCAAAGGAAAATATCAATACCATTGCACGTAAGGGCCTAAAGGCCGATCTGCCAGCTGTTCATAGGATTGTTGATC
ACTTTTACTGGACTCAAGAGGATATGGAGGCTGTCATGCTTGACATCAATCAGGGCATGACACCAGAAGCCGCAGCCAAG
AAATGGGTGGCCAGCCATGCTGATAAGGTGGCCAAATGGACTCAGTCATAA

Upstream 100 bases:

>100_bases
AGCAGCACCGCTTGTTAGGGGTTATTATTCGTGGGCGTGTCCTTGAAGCCTTGGCTAATATTCCAGATGACGACCTTAAT
TAGAATTGAGAGACTGTATT

Downstream 100 bases:

>100_bases
GGCTGCTCGCTCAGATGGAGCTTAGCCTAATCATGGATTTTGCAGAAGGCTTTCCGTCCATATCAGTTGAGGAAAGTCGT
TGTATAACAGATGATGTCAT

Product: glycine betaine transport system permease protein OpuAB

Products: ADP; phosphate; L-proline [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 576; Mature: 576

Protein sequence:

>576_residues
MENLLQTTLPVAQLVEQLTEWLTKTFSGFFDLLQLVGNTLMDWITQTLLFINPLLFMLLVTCAMFFLARKKWPLPIFTLL
GLLFVYNQGLWAELINTFTLVLVASLISVLLGIPLGIWMAKSKTVHQVINPMLDLMQTMPAFVYLIPAVAFFGIGMVPGV
FASVIFALPPTVRFTNLAIRHIPTELVEASDAFGSTPKQKLLKVELPLAKHTIMAGVNQTMMLALSMVVTGSMIGAPGLG
REVLSALQHADIGRGFVSGLALVILAIILDRMTQHFNGKPQERTQTGKTKKWLGLAALAVFLLSALGRGFAAMSSSSSDK
GQKVTIAYVQWDSEVASTHVIAQVLRDEGYQVTLTPLDNAVMWQTIANGDADFSTSAWLPVTHQQQYQKYQDKLDNLGPN
LKGTKLGLAVPAYMSDVNSIEELSDQANQQIIGIEPGAGIMTAADKTQRAYSNLADWELIAASTGAMTTSLDQAIKKKEP
IVVTAWSPHWIFAKYDLKYLADPKKTFGSKENINTIARKGLKADLPAVHRIVDHFYWTQEDMEAVMLDINQGMTPEAAAK
KWVASHADKVAKWTQS

Sequences:

>Translated_576_residues
MENLLQTTLPVAQLVEQLTEWLTKTFSGFFDLLQLVGNTLMDWITQTLLFINPLLFMLLVTCAMFFLARKKWPLPIFTLL
GLLFVYNQGLWAELINTFTLVLVASLISVLLGIPLGIWMAKSKTVHQVINPMLDLMQTMPAFVYLIPAVAFFGIGMVPGV
FASVIFALPPTVRFTNLAIRHIPTELVEASDAFGSTPKQKLLKVELPLAKHTIMAGVNQTMMLALSMVVTGSMIGAPGLG
REVLSALQHADIGRGFVSGLALVILAIILDRMTQHFNGKPQERTQTGKTKKWLGLAALAVFLLSALGRGFAAMSSSSSDK
GQKVTIAYVQWDSEVASTHVIAQVLRDEGYQVTLTPLDNAVMWQTIANGDADFSTSAWLPVTHQQQYQKYQDKLDNLGPN
LKGTKLGLAVPAYMSDVNSIEELSDQANQQIIGIEPGAGIMTAADKTQRAYSNLADWELIAASTGAMTTSLDQAIKKKEP
IVVTAWSPHWIFAKYDLKYLADPKKTFGSKENINTIARKGLKADLPAVHRIVDHFYWTQEDMEAVMLDINQGMTPEAAAK
KWVASHADKVAKWTQS
>Mature_576_residues
MENLLQTTLPVAQLVEQLTEWLTKTFSGFFDLLQLVGNTLMDWITQTLLFINPLLFMLLVTCAMFFLARKKWPLPIFTLL
GLLFVYNQGLWAELINTFTLVLVASLISVLLGIPLGIWMAKSKTVHQVINPMLDLMQTMPAFVYLIPAVAFFGIGMVPGV
FASVIFALPPTVRFTNLAIRHIPTELVEASDAFGSTPKQKLLKVELPLAKHTIMAGVNQTMMLALSMVVTGSMIGAPGLG
REVLSALQHADIGRGFVSGLALVILAIILDRMTQHFNGKPQERTQTGKTKKWLGLAALAVFLLSALGRGFAAMSSSSSDK
GQKVTIAYVQWDSEVASTHVIAQVLRDEGYQVTLTPLDNAVMWQTIANGDADFSTSAWLPVTHQQQYQKYQDKLDNLGPN
LKGTKLGLAVPAYMSDVNSIEELSDQANQQIIGIEPGAGIMTAADKTQRAYSNLADWELIAASTGAMTTSLDQAIKKKEP
IVVTAWSPHWIFAKYDLKYLADPKKTFGSKENINTIARKGLKADLPAVHRIVDHFYWTQEDMEAVMLDINQGMTPEAAAK
KWVASHADKVAKWTQS

Specific function: Involved in a multicomponent binding-protein-dependent transport system for glycine betaine; probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4176

COG function: function code E; ABC-type proline/glycine betaine transport system, permease component

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1789033, Length=292, Percent_Identity=41.7808219178082, Blast_Score=208, Evalue=9e-55,
Organism=Escherichia coli, GI1788451, Length=191, Percent_Identity=29.8429319371728, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1788449, Length=198, Percent_Identity=28.2828282828283, Blast_Score=69, Evalue=6e-13,
Organism=Escherichia coli, GI1786564, Length=158, Percent_Identity=29.746835443038, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 63367; Mature: 63367

Theoretical pI: Translated: 7.87; Mature: 7.87

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure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HHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-proline [Periplasm]; H2O [C]

Specific reaction: ATP + L-proline [Periplasm] + H2O = ADP + phosphate + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7622480; 8969502; 9384377 [H]