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The map label for this gene is pbpF [H]

Identifier: 195977305

GI number: 195977305

Start: 169909

End: 172230

Strand: Direct

Name: pbpF [H]

Synonym: Sez_0158

Alternate gene names: 195977305

Gene position: 169909-172230 (Clockwise)

Preceding gene: 195977304

Following gene: 195977306

Centisome position: 8.39

GC content: 44.79

Gene sequence:

>2322_bases
ATGACATTTTTAGAATTATTACAAAAGAAATTTTTTCCAAAAAAATACCAAGAAAAACAGGCCTTAAAGGAAAGGGCAGC
GCAGCATATGGCTGACAAAGAGGCTGCTGTTGCTGCTTTGGCCGATCAGGAGGCACAGGCAGGGCAGACTAGGGGGCAAT
CAGTCCTTCAACGCAGCAAGTCTTATGGGGCTAAAAGGAAAAAGCAGCCGGTCTGGCTTCAAAGGCTAGAGGCGATTTTG
CCATCGTCACAAAACCCTATTCGCCGCTTTCTTCGTCGGTACCATATTGGTAAAATCCTGTTGATTTTGATGGGTACCTT
TGTCTTGTTGCTGGGGTCTTACCTTTTTTACCTCTCAAAGACAGCCAAGGTTTCTGATTTGCAGGACGCTCTTAAGGCGA
CGACAGTCATCTATGACCGCAAGGGAGAATATGCAGGCAGTCTTTCTGGTCAAAAGGGAACTTATGTAGAGCTTGACGCT
ATTTCAGATGATTTGGAAAAGGCGGTTATTGCCACAGAGGACCGTACCTTTTATACCAACAATGGTATTAATTTCAAGCG
CTTTTTATTGGCTATTGTGACCGCAGGCCGCTTTGGTGGAGGCTCGACCATTACTCAGCAGTTGGCTAAAAATGCTTATT
TGTCTCAGGAGCAGAGCATTAAAAGAAAGGCGCGTGAGTTTTTCTTAGCACTTGAATTGACCAAAAAATACAGTAAAAAT
GACATTCTGACCATGTACCTGAATAATTCCTACTTTGGTAATGGTATTTGGGGTGTTGAGGACGCCAGCCAAAAATATTT
TGGTACCTCAGCAGCTAATCTAACCTTGGACGAGGCAGCAACCCTTGCCGGTATGCTCAAGGGACCTGAAATTTACAATC
CCTATTACTCAATCGAAAATGCAACTAATCGTAGAGATACCGTTTTGTCAGCTATGGTTGACGCTGGGAAAATCAGCAAG
CAGCAGGCACAAGAGGCCCAAGCTATTGGTATGGGCAACCGTTTAGCAGACACCTATGTCGGCAAGACAGATGATTATCA
GTACCCATCTTATTTTGATGCTGTTATCAATGAGGCTATCTCGCGCTATGGCATCTCAGAAAAGGATATTGTCAATGACG
GCTACAAGATTTACACAGAGTTAGATCAAAATTACCAAGCAGGCATGCAGACCACCTTTAATGAGGACAGTTTATTTCCT
GTGTCTGAGTTTGACGGCAGCTCAGCTGAGGCAGCTAGTGTGGCTCTAGATCCTAAAACCGGGGCTGTCAGAGGGCTTGT
TGGTCGTGTCAATAGCACAGAAAACCAGCCCTTTAGAAGCTTTAACTATGCTACTCAAGCCAAGCGCAGCCCTGCTTCCA
CGATCAAGCCACTAGTTGTTTATACTCCTGCTGTTGCAGCAGGCTGGTCGCTGGAGCACGAACTGCCAAACAGCGTTCAG
GATTTTGGCGGCTATCAGCCGCATAATTATGGCAATTACGAATCAGAAACAGTTCCTATGTATCAGGCTTTGGCTAATTC
CTACAATATTCCTGCTGTTTCAATTCTGGATCAGTTGGGGGTGGATAAGGCCTTTAGCTATGGTGAAAAATTTGGTTTGA
AGATGAGTGATACCAAAAAGGAGCTGGGAGTGGCTCTTGGTAGTGGTGTCACCACCAATCCGCTTGAGATGGCACAGGCC
TATGCAGTCTTTGCCAATGGCGGTGTCAAGCATATTGCCCACCTGATTACCAGAATTGAAAATGCTAAGGGCAAGGTTAT
CAAGACCTTTACAGACAAAACCAAGCGGGTTATCAGCCAATCAGTGGCTGATAAGATGACTAGCATGATGCTTGGAACCT
TTTCCAATGGTACAGCTGTTAATGCCAATGTCTATGGCTATACTCTGGCTGGTAAAACAGGGACCACAGAAACTGACTTT
AACCCGGACCTTTCCGGTGATCAGTGGGTCATTGGCTACACGCCAGACGTTGTCATCAGTCAATGGGTCGGCTTCAATCA
AACCGATGAGCATCATTATTTATCAGATTCAAGCGCGGGCACTGCCTCAGCAATTTTTAGCACACAAGCTTCCTATATAT
TGCCATATACCAAAGGAAGTCAATTCAAGATCGATAACGCTTACGCTCAAAACGGTATTTCAGCGGTGTATGGGATTAAT
GAGACCAATACACAAGCGGGTATTGATTCTCAATCCATCATTGATAGTCTTAGAAAGTCAGCCGAGGAAGCTTCAAAGTC
TATTTCAGAGGCAGTTGATCAATCTGGTCTCAGAGAAAAGGCGCAAAGTATCTGGAAGAGTATTGTTGACTACTTTAGAT
AG

Upstream 100 bases:

>100_bases
CCAGACGGCTTGCTATTTCAGGTAGATGGGGTTGCTTATTCAAAGCATATCTCCGTATCCTAAACAATATGACTTGGAGG
CTTAGTAGTATCATAAGGCT

Downstream 100 bases:

>100_bases
CTTGCGAAAATATCTAAATCGTGTTAGAATAATCACTACGGAGGCGTTATGGCACAGAAAAAAGCAAGCCTAGCGTGTGT
AGAATGTGGCAGCCGCAATT

Product: multimodular transpeptidase-transglycosylase

Products: NA

Alternate protein names: PBP-1F; Penicillin-binding protein F [H]

Number of amino acids: Translated: 773; Mature: 772

Protein sequence:

>773_residues
MTFLELLQKKFFPKKYQEKQALKERAAQHMADKEAAVAALADQEAQAGQTRGQSVLQRSKSYGAKRKKQPVWLQRLEAIL
PSSQNPIRRFLRRYHIGKILLILMGTFVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDRKGEYAGSLSGQKGTYVELDA
ISDDLEKAVIATEDRTFYTNNGINFKRFLLAIVTAGRFGGGSTITQQLAKNAYLSQEQSIKRKAREFFLALELTKKYSKN
DILTMYLNNSYFGNGIWGVEDASQKYFGTSAANLTLDEAATLAGMLKGPEIYNPYYSIENATNRRDTVLSAMVDAGKISK
QQAQEAQAIGMGNRLADTYVGKTDDYQYPSYFDAVINEAISRYGISEKDIVNDGYKIYTELDQNYQAGMQTTFNEDSLFP
VSEFDGSSAEAASVALDPKTGAVRGLVGRVNSTENQPFRSFNYATQAKRSPASTIKPLVVYTPAVAAGWSLEHELPNSVQ
DFGGYQPHNYGNYESETVPMYQALANSYNIPAVSILDQLGVDKAFSYGEKFGLKMSDTKKELGVALGSGVTTNPLEMAQA
YAVFANGGVKHIAHLITRIENAKGKVIKTFTDKTKRVISQSVADKMTSMMLGTFSNGTAVNANVYGYTLAGKTGTTETDF
NPDLSGDQWVIGYTPDVVISQWVGFNQTDEHHYLSDSSAGTASAIFSTQASYILPYTKGSQFKIDNAYAQNGISAVYGIN
ETNTQAGIDSQSIIDSLRKSAEEASKSISEAVDQSGLREKAQSIWKSIVDYFR

Sequences:

>Translated_773_residues
MTFLELLQKKFFPKKYQEKQALKERAAQHMADKEAAVAALADQEAQAGQTRGQSVLQRSKSYGAKRKKQPVWLQRLEAIL
PSSQNPIRRFLRRYHIGKILLILMGTFVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDRKGEYAGSLSGQKGTYVELDA
ISDDLEKAVIATEDRTFYTNNGINFKRFLLAIVTAGRFGGGSTITQQLAKNAYLSQEQSIKRKAREFFLALELTKKYSKN
DILTMYLNNSYFGNGIWGVEDASQKYFGTSAANLTLDEAATLAGMLKGPEIYNPYYSIENATNRRDTVLSAMVDAGKISK
QQAQEAQAIGMGNRLADTYVGKTDDYQYPSYFDAVINEAISRYGISEKDIVNDGYKIYTELDQNYQAGMQTTFNEDSLFP
VSEFDGSSAEAASVALDPKTGAVRGLVGRVNSTENQPFRSFNYATQAKRSPASTIKPLVVYTPAVAAGWSLEHELPNSVQ
DFGGYQPHNYGNYESETVPMYQALANSYNIPAVSILDQLGVDKAFSYGEKFGLKMSDTKKELGVALGSGVTTNPLEMAQA
YAVFANGGVKHIAHLITRIENAKGKVIKTFTDKTKRVISQSVADKMTSMMLGTFSNGTAVNANVYGYTLAGKTGTTETDF
NPDLSGDQWVIGYTPDVVISQWVGFNQTDEHHYLSDSSAGTASAIFSTQASYILPYTKGSQFKIDNAYAQNGISAVYGIN
ETNTQAGIDSQSIIDSLRKSAEEASKSISEAVDQSGLREKAQSIWKSIVDYFR
>Mature_772_residues
TFLELLQKKFFPKKYQEKQALKERAAQHMADKEAAVAALADQEAQAGQTRGQSVLQRSKSYGAKRKKQPVWLQRLEAILP
SSQNPIRRFLRRYHIGKILLILMGTFVLLLGSYLFYLSKTAKVSDLQDALKATTVIYDRKGEYAGSLSGQKGTYVELDAI
SDDLEKAVIATEDRTFYTNNGINFKRFLLAIVTAGRFGGGSTITQQLAKNAYLSQEQSIKRKAREFFLALELTKKYSKND
ILTMYLNNSYFGNGIWGVEDASQKYFGTSAANLTLDEAATLAGMLKGPEIYNPYYSIENATNRRDTVLSAMVDAGKISKQ
QAQEAQAIGMGNRLADTYVGKTDDYQYPSYFDAVINEAISRYGISEKDIVNDGYKIYTELDQNYQAGMQTTFNEDSLFPV
SEFDGSSAEAASVALDPKTGAVRGLVGRVNSTENQPFRSFNYATQAKRSPASTIKPLVVYTPAVAAGWSLEHELPNSVQD
FGGYQPHNYGNYESETVPMYQALANSYNIPAVSILDQLGVDKAFSYGEKFGLKMSDTKKELGVALGSGVTTNPLEMAQAY
AVFANGGVKHIAHLITRIENAKGKVIKTFTDKTKRVISQSVADKMTSMMLGTFSNGTAVNANVYGYTLAGKTGTTETDFN
PDLSGDQWVIGYTPDVVISQWVGFNQTDEHHYLSDSSAGTASAIFSTQASYILPYTKGSQFKIDNAYAQNGISAVYGINE
TNTQAGIDSQSIIDSLRKSAEEASKSISEAVDQSGLREKAQSIWKSIVDYFR

Specific function: Cell wall formation. May be involved in outgrowth of the germinated spore or it could function in the synthesis of the germ cell wall [H]

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=538, Percent_Identity=31.4126394052045, Blast_Score=183, Evalue=5e-47,
Organism=Escherichia coli, GI87082258, Length=315, Percent_Identity=33.968253968254, Blast_Score=172, Evalue=7e-44,
Organism=Escherichia coli, GI1788867, Length=524, Percent_Identity=28.4351145038168, Blast_Score=132, Evalue=1e-31,
Organism=Escherichia coli, GI1789601, Length=139, Percent_Identity=41.726618705036, Blast_Score=95, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011816
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: 2.4.1.129; 3.4.-.- [C]

Molecular weight: Translated: 85070; Mature: 84939

Theoretical pI: Translated: 8.28; Mature: 8.28

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTFLELLQKKFFPKKYQEKQALKERAAQHMADKEAAVAALADQEAQAGQTRGQSVLQRSK
CCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYGAKRKKQPVWLQRLEAILPSSQNPIRRFLRRYHIGKILLILMGTFVLLLGSYLFYLSK
HHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TAKVSDLQDALKATTVIYDRKGEYAGSLSGQKGTYVELDAISDDLEKAVIATEDRTFYTN
CCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHEEECCCEEEEC
NGINFKRFLLAIVTAGRFGGGSTITQQLAKNAYLSQEQSIKRKAREFFLALELTKKYSKN
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DILTMYLNNSYFGNGIWGVEDASQKYFGTSAANLTLDEAATLAGMLKGPEIYNPYYSIEN
CEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCC
ATNRRDTVLSAMVDAGKISKQQAQEAQAIGMGNRLADTYVGKTDDYQYPSYFDAVINEAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHH
SRYGISEKDIVNDGYKIYTELDQNYQAGMQTTFNEDSLFPVSEFDGSSAEAASVALDPKT
HHCCCCHHHHHCCCCEEEEHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
GAVRGLVGRVNSTENQPFRSFNYATQAKRSPASTIKPLVVYTPAVAAGWSLEHELPNSVQ
CHHHHHHHCCCCCCCCCCHHCCHHHHHCCCCHHHCCCEEEECCCHHCCCCCCHHCCCHHH
DFGGYQPHNYGNYESETVPMYQALANSYNIPAVSILDQLGVDKAFSYGEKFGLKMSDTKK
HHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCHH
ELGVALGSGVTTNPLEMAQAYAVFANGGVKHIAHLITRIENAKGKVIKTFTDKTKRVISQ
HHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHH
SVADKMTSMMLGTFSNGTAVNANVYGYTLAGKTGTTETDFNPDLSGDQWVIGYTPDVVIS
HHHHHHHHHHHHCCCCCCEEECEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCHHHHHH
QWVGFNQTDEHHYLSDSSAGTASAIFSTQASYILPYTKGSQFKIDNAYAQNGISAVYGIN
HHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCEEECHHHHHCCCEEEECCC
ETNTQAGIDSQSIIDSLRKSAEEASKSISEAVDQSGLREKAQSIWKSIVDYFR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
TFLELLQKKFFPKKYQEKQALKERAAQHMADKEAAVAALADQEAQAGQTRGQSVLQRSK
CHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SYGAKRKKQPVWLQRLEAILPSSQNPIRRFLRRYHIGKILLILMGTFVLLLGSYLFYLSK
HHCCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
TAKVSDLQDALKATTVIYDRKGEYAGSLSGQKGTYVELDAISDDLEKAVIATEDRTFYTN
CCHHHHHHHHHHHHHHEEECCCCCCCCCCCCCCCEEEECCHHHHHHHHHEEECCCEEEEC
NGINFKRFLLAIVTAGRFGGGSTITQQLAKNAYLSQEQSIKRKAREFFLALELTKKYSKN
CCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
DILTMYLNNSYFGNGIWGVEDASQKYFGTSAANLTLDEAATLAGMLKGPEIYNPYYSIEN
CEEEEEECCCCCCCCCCCCCCCCHHHCCCCCCCEEHHHHHHHHHHHCCCCCCCCCCCCCC
ATNRRDTVLSAMVDAGKISKQQAQEAQAIGMGNRLADTYVGKTDDYQYPSYFDAVINEAI
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCCCCCHHHHHHHHHHH
SRYGISEKDIVNDGYKIYTELDQNYQAGMQTTFNEDSLFPVSEFDGSSAEAASVALDPKT
HHCCCCHHHHHCCCCEEEEHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCC
GAVRGLVGRVNSTENQPFRSFNYATQAKRSPASTIKPLVVYTPAVAAGWSLEHELPNSVQ
CHHHHHHHCCCCCCCCCCHHCCHHHHHCCCCHHHCCCEEEECCCHHCCCCCCHHCCCHHH
DFGGYQPHNYGNYESETVPMYQALANSYNIPAVSILDQLGVDKAFSYGEKFGLKMSDTKK
HHCCCCCCCCCCCCCCCHHHHHHHHCCCCCCHHHHHHHHCHHHHHHHHHHCCCCCCCCHH
ELGVALGSGVTTNPLEMAQAYAVFANGGVKHIAHLITRIENAKGKVIKTFTDKTKRVISQ
HHHHHHCCCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCEEEHHHHHHHHHHHH
SVADKMTSMMLGTFSNGTAVNANVYGYTLAGKTGTTETDFNPDLSGDQWVIGYTPDVVIS
HHHHHHHHHHHHCCCCCCEEECEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCHHHHHH
QWVGFNQTDEHHYLSDSSAGTASAIFSTQASYILPYTKGSQFKIDNAYAQNGISAVYGIN
HHCCCCCCCCCCCCCCCCCCCHHHHHHCCCCEEEEECCCCCEEECHHHHHCCCEEEECCC
ETNTQAGIDSQSIIDSLRKSAEEASKSISEAVDQSGLREKAQSIWKSIVDYFR
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377; 8335642; 1459957 [H]