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The map label for this gene is rpoA [H]

Identifier: 195977234

GI number: 195977234

Start: 82533

End: 83471

Strand: Direct

Name: rpoA [H]

Synonym: Sez_0082

Alternate gene names: 195977234

Gene position: 82533-83471 (Clockwise)

Preceding gene: 195977233

Following gene: 195977235

Centisome position: 4.08

GC content: 38.34

Gene sequence:

>939_bases
ATGATTGAGTTTGAAAAACCAATAATAACAAAAATTGATGAAAATAAAGATTACGGTAGATTTGTAATCGAACCACTTGA
ACGTGGATATGGTACAACTCTAGGTAATTCACTTCGTCGTGTACTCTTGTCTTCACTTCCAGGTGCAGCTGTTACATCAA
TCAGAATTGATGGAGTACTACACGAATTTGATACAATCCCAGGTGTACGTGAAGATGTCATGCAAATTATCCTTAATGTT
AAGGGACTTGCTGTGAAATCATACGTCGAAGACGAAAAGATTATCGAGCTTGATGTAGAAGGCCCAGCAGAGGTAACAGC
TGGAGATATTCTCACAGATAGCGATATCGAACTTGTTAACCCTGATCATTATCTTTTTACTATTGCTGATGGTTATACAT
TGAAGGCTTCAATGACTGTCGCTAAAAAACGCGGCTATGTACCAGCAGAGGGTAATAAAAAAGATGATGCACCTGTGGGG
ACGTTGGCAGTAGATTCAATCTATACGCCAGTAAAAAAGGTTAATTATCAAGTTGAGCCTGCTCGTGTAGGTAGTAATGA
CGGCTTTGATAAATTGACTATTGAAATCATGACAAACGGAACAATTATTCCCGAAGATGCCTTAGGTTTATCTGCTCGAG
TTTTAATCGAACACTTGAATTTATTTACTGATTTAACAGATGTTGCAAAGGCAACTGAAGTGATGAAGGAAGCTGAAAAA
GTGAACGATGAAAAGGTACTTGATCGCACTATTGAGGAACTTGATTTGTCCGTACGCTCATATAACTGCTTGAAACGTGC
CGGTATTAATACTGTCTTCGATTTAACAGAAAAATCTGAGCCTGAAATGATGAAGGTCCGTAACCTTGGACGTAAGAGTC
TTGAGGAAGTAAAGGTCAAGCTTGCTGACTTAGGTTTGGGACTAAAAAACGATAAATAA

Upstream 100 bases:

>100_bases
GTGACTCCTGTACCGCATAATGGTGCTCGTCCTCCAAAACGTCGTCGTGTATAATCACAGTAACAAATAGTACACAAGTT
TCGTTTCGAGGGGTGAAAGA

Downstream 100 bases:

>100_bases
CATAGGAGGATAAAATGGCTTACCGTAAACTAGGACGCACTAGCTCACAACGTAAAGCAATGCTTCGTGACTTAACGACA
GATCTTTTGATTAACGAATC

Product: DNA-directed RNA polymerase subunit alpha

Products: NA

Alternate protein names: RNAP subunit alpha; RNA polymerase subunit alpha; Transcriptase subunit alpha [H]

Number of amino acids: Translated: 312; Mature: 312

Protein sequence:

>312_residues
MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLSSLPGAAVTSIRIDGVLHEFDTIPGVREDVMQIILNV
KGLAVKSYVEDEKIIELDVEGPAEVTAGDILTDSDIELVNPDHYLFTIADGYTLKASMTVAKKRGYVPAEGNKKDDAPVG
TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEHLNLFTDLTDVAKATEVMKEAEK
VNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLTEKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK

Sequences:

>Translated_312_residues
MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLSSLPGAAVTSIRIDGVLHEFDTIPGVREDVMQIILNV
KGLAVKSYVEDEKIIELDVEGPAEVTAGDILTDSDIELVNPDHYLFTIADGYTLKASMTVAKKRGYVPAEGNKKDDAPVG
TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEHLNLFTDLTDVAKATEVMKEAEK
VNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLTEKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK
>Mature_312_residues
MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLSSLPGAAVTSIRIDGVLHEFDTIPGVREDVMQIILNV
KGLAVKSYVEDEKIIELDVEGPAEVTAGDILTDSDIELVNPDHYLFTIADGYTLKASMTVAKKRGYVPAEGNKKDDAPVG
TLAVDSIYTPVKKVNYQVEPARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEHLNLFTDLTDVAKATEVMKEAEK
VNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLTEKSEPEMMKVRNLGRKSLEEVKVKLADLGLGLKNDK

Specific function: DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates [H]

COG id: COG0202

COG function: function code K; DNA-directed RNA polymerase, alpha subunit/40 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA polymerase alpha chain family [H]

Homologues:

Organism=Escherichia coli, GI1789690, Length=311, Percent_Identity=45.9807073954984, Blast_Score=263, Evalue=1e-71,

Paralogues:

None

Copy number: 850 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 560 Molecules/Cell In: Stationary-Phase, Rich-Media (Based on E. coli). 6847 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 7,000 Molecules/Cell In: Glucose minimal media

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011261
- InterPro:   IPR011262
- InterPro:   IPR009025
- InterPro:   IPR011263
- InterPro:   IPR011260
- InterPro:   IPR011773
- ProDom:   PD001179 [H]

Pfam domain/function: PF01000 RNA_pol_A_bac; PF03118 RNA_pol_A_CTD; PF01193 RNA_pol_L [H]

EC number: =2.7.7.6 [H]

Molecular weight: Translated: 34484; Mature: 34484

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLSSLPGAAVTSIRIDGVL
CCCCCCCHHEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHH
HEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGPAEVTAGDILTDSDIELVN
HHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCCCCCCCEEECCCCEEEC
PDHYLFTIADGYTLKASMTVAKKRGYVPAEGNKKDDAPVGTLAVDSIYTPVKKVNYQVEP
CCEEEEEEECCEEEEEEEEEHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCEEECC
ARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEHLNLFTDLTDVAKATEVMKEAEK
CCCCCCCCCCEEEEEEEECCEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLTEKSEPEMMKVRNLGRKSLEEVKVK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHH
LADLGLGLKNDK
HHHHCCCCCCCC
>Mature Secondary Structure
MIEFEKPIITKIDENKDYGRFVIEPLERGYGTTLGNSLRRVLLSSLPGAAVTSIRIDGVL
CCCCCCCHHEEECCCCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCEEEEEEHHHH
HEFDTIPGVREDVMQIILNVKGLAVKSYVEDEKIIELDVEGPAEVTAGDILTDSDIELVN
HHHHCCCCHHHHHHHHHHHCCCHHHHHHCCCCEEEEEECCCCCCCCCCCEEECCCCEEEC
PDHYLFTIADGYTLKASMTVAKKRGYVPAEGNKKDDAPVGTLAVDSIYTPVKKVNYQVEP
CCEEEEEEECCEEEEEEEEEHHHCCCCCCCCCCCCCCCCCEEEHHHHHHHHHHCCEEECC
ARVGSNDGFDKLTIEIMTNGTIIPEDALGLSARVLIEHLNLFTDLTDVAKATEVMKEAEK
CCCCCCCCCCEEEEEEEECCEECCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VNDEKVLDRTIEELDLSVRSYNCLKRAGINTVFDLTEKSEPEMMKVRNLGRKSLEEVKVK
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCHHHHHHHCCHHHHHHHHHH
LADLGLGLKNDK
HHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA