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The map label for this gene is thnsl [H]

Identifier: 195977204

GI number: 195977204

Start: 65600

End: 67075

Strand: Direct

Name: thnsl [H]

Synonym: Sez_0052

Alternate gene names: 195977204

Gene position: 65600-67075 (Clockwise)

Preceding gene: 195977203

Following gene: 195977205

Centisome position: 3.24

GC content: 43.77

Gene sequence:

>1476_bases
ATGTACCAATCAACAAGAAATGCAGCTAATAAGGTAACGGCTAGTCAAGCGATTTTAGGTGGCCTAGCAGACGATGGTGG
CTTGTTTACCCCTACAAGCATGCCAGTGTTGGATCTAGATTTTGAGGAGCTAAAAACCAAGTCCTATCAAGAGGTTACCA
AGCTTATTTTATCAGCCTTTTTTGATGACTATACCGAAGCAGAGTTAGATGCTTGTATCTCTTCTGCCTATGATGATAAG
TTTGATACAGCAGTGATAGCACCTCTAGCTACGTTAAAGGATTATCATAATTTGGAGCTATTTCACGGCCCAACCATTGC
ATTTAAAGACATGGCTCTATCTATTTTGCCTTATCTCTTGACAACTGCTGCTAAAAAGCAGGGTGTAGAGCGAAAAATTG
CGATTTTAACCGCTACCTCAGGTGATACTGGTAAGGCAGCGATGGCAGGTTTTGCTGATGTTCCGGGAACGGATATTATT
GTCTTTTATCCTAAGTCAGGCGTTAGCAGGATTCAGGAGCTGCAGATGATCACACAGGCCGGTCACAATACCCATGTTGT
GGCGATTGAAGGCAATTTTGATGATGCTCAAACAGAGGTCAAGCGCCTGTTTAATGACCCTGATTTGCGTGAGCGGCTAG
CAGCTCAACAGATACAGCTGTCTTCGGCCAATTCGATGAATATTGGTCGTTTGATTCCTCAGGTTGTCTATTATATTTAT
GCTTATGCCCAGTTGGTAAGGCATCAGCGACTTGAAGCTGGAGAGACGATTAACATAGTAGTGCCGACAGGAAATTTTGG
CAATCTACTGGCTGCCTACTACGCCAAAGAAATTGGACTTCCTGTTGCCAAATTCATCTGTGCTTCCAATGAAAACAATG
TCTTGACAGATTTCTTTGCTAGTGGTAGGTATGATAAAAAGCGTCCCTTCAAGGTGACTACTAGTCCATCAATGGATATT
TTGGTGTCTTCAAATTTAGAGCGCTTGGTTTTTCATTTACTAGGCAATGATGCCCAAAAGACAGCAGTATTAATGAAGAG
CTTGGCAGAAACAGGTCAGTATCAGCTAGCTGATGCAGATCAGTCTATCCTTGATTTATTTGCGGCAGGCTTTGCAACAG
AGCAAGAGACTGCTAAAGAGATCAAAACGATATTTGAGTCTGATCATTATGTGCTTGACCCACATACAGCTGTAGCTTCA
GCTGTTTATCGTGCTTATCGGGAGCAATCAGGTGACTCTAGACCGACTATTGTTGCGTCAACTGCTAGTCCTTATAAGTT
TCCGCGGGTAGTTGTGGAAGCTATTAGTGGTCAGCAGGTTGCCAGTGATTTTGAGGCGCTTGAGCAGTTGGCTGATATAT
CAGGTGTAGTCGTGCCACAAGCGGTCAATGGCTTACAGGAGGCGCCCATTCGTCATAGCCTTTTAGTGACAGTAGCAGAT
ATGCAGCAGGCAGTAGAGGCTTATCTTGGAGTATAG

Upstream 100 bases:

>100_bases
AGCACAGAAAATGGCTGTCTCAAAATCCTAGAAGGCTTATGATTGGAATATGATATAATAGTCAAAAGAAATGATGAGGT
GATGTGAAGCAATGCCAATG

Downstream 100 bases:

>100_bases
GAGGCATAAAGGAGCTCCCTTTCAATTGATATGAATATAATGACTCACAATAGAAAAAAGATACTGTCCTTGGCCCTTCC
ATCAATGGCAGAAAATATAT

Product: threonine synthase

Products: NA

Alternate protein names: TS [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MYQSTRNAANKVTASQAILGGLADDGGLFTPTSMPVLDLDFEELKTKSYQEVTKLILSAFFDDYTEAELDACISSAYDDK
FDTAVIAPLATLKDYHNLELFHGPTIAFKDMALSILPYLLTTAAKKQGVERKIAILTATSGDTGKAAMAGFADVPGTDII
VFYPKSGVSRIQELQMITQAGHNTHVVAIEGNFDDAQTEVKRLFNDPDLRERLAAQQIQLSSANSMNIGRLIPQVVYYIY
AYAQLVRHQRLEAGETINIVVPTGNFGNLLAAYYAKEIGLPVAKFICASNENNVLTDFFASGRYDKKRPFKVTTSPSMDI
LVSSNLERLVFHLLGNDAQKTAVLMKSLAETGQYQLADADQSILDLFAAGFATEQETAKEIKTIFESDHYVLDPHTAVAS
AVYRAYREQSGDSRPTIVASTASPYKFPRVVVEAISGQQVASDFEALEQLADISGVVVPQAVNGLQEAPIRHSLLVTVAD
MQQAVEAYLGV

Sequences:

>Translated_491_residues
MYQSTRNAANKVTASQAILGGLADDGGLFTPTSMPVLDLDFEELKTKSYQEVTKLILSAFFDDYTEAELDACISSAYDDK
FDTAVIAPLATLKDYHNLELFHGPTIAFKDMALSILPYLLTTAAKKQGVERKIAILTATSGDTGKAAMAGFADVPGTDII
VFYPKSGVSRIQELQMITQAGHNTHVVAIEGNFDDAQTEVKRLFNDPDLRERLAAQQIQLSSANSMNIGRLIPQVVYYIY
AYAQLVRHQRLEAGETINIVVPTGNFGNLLAAYYAKEIGLPVAKFICASNENNVLTDFFASGRYDKKRPFKVTTSPSMDI
LVSSNLERLVFHLLGNDAQKTAVLMKSLAETGQYQLADADQSILDLFAAGFATEQETAKEIKTIFESDHYVLDPHTAVAS
AVYRAYREQSGDSRPTIVASTASPYKFPRVVVEAISGQQVASDFEALEQLADISGVVVPQAVNGLQEAPIRHSLLVTVAD
MQQAVEAYLGV
>Mature_491_residues
MYQSTRNAANKVTASQAILGGLADDGGLFTPTSMPVLDLDFEELKTKSYQEVTKLILSAFFDDYTEAELDACISSAYDDK
FDTAVIAPLATLKDYHNLELFHGPTIAFKDMALSILPYLLTTAAKKQGVERKIAILTATSGDTGKAAMAGFADVPGTDII
VFYPKSGVSRIQELQMITQAGHNTHVVAIEGNFDDAQTEVKRLFNDPDLRERLAAQQIQLSSANSMNIGRLIPQVVYYIY
AYAQLVRHQRLEAGETINIVVPTGNFGNLLAAYYAKEIGLPVAKFICASNENNVLTDFFASGRYDKKRPFKVTTSPSMDI
LVSSNLERLVFHLLGNDAQKTAVLMKSLAETGQYQLADADQSILDLFAAGFATEQETAKEIKTIFESDHYVLDPHTAVAS
AVYRAYREQSGDSRPTIVASTASPYKFPRVVVEAISGQQVASDFEALEQLADISGVVVPQAVNGLQEAPIRHSLLVTVAD
MQQAVEAYLGV

Specific function: Catalyzes the gamma-elimination of phosphate from L- phosphohomoserine and the beta-addition of water to produce L- threonine [H]

COG id: COG0498

COG function: function code E; Threonine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the threonine synthase family [H]

Homologues:

Organism=Homo sapiens, GI153792148, Length=451, Percent_Identity=39.0243902439024, Blast_Score=293, Evalue=3e-79,
Organism=Homo sapiens, GI149193323, Length=454, Percent_Identity=32.15859030837, Blast_Score=214, Evalue=1e-55,
Organism=Escherichia coli, GI1786185, Length=430, Percent_Identity=33.953488372093, Blast_Score=199, Evalue=5e-52,
Organism=Saccharomyces cerevisiae, GI6319901, Length=471, Percent_Identity=35.244161358811, Blast_Score=263, Evalue=4e-71,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR004450 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: =4.2.3.1 [H]

Molecular weight: Translated: 53524; Mature: 53524

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQSTRNAANKVTASQAILGGLADDGGLFTPTSMPVLDLDFEELKTKSYQEVTKLILSAF
CCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEECCHHHHHCCHHHHHHHHHHHHH
FDDYTEAELDACISSAYDDKFDTAVIAPLATLKDYHNLELFHGPTIAFKDMALSILPYLL
HHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
TTAAKKQGVERKIAILTATSGDTGKAAMAGFADVPGTDIIVFYPKSGVSRIQELQMITQA
HHHHHHCCCCEEEEEEEECCCCCCCHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHC
GHNTHVVAIEGNFDDAQTEVKRLFNDPDLRERLAAQQIQLSSANSMNIGRLIPQVVYYIY
CCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AYAQLVRHQRLEAGETINIVVPTGNFGNLLAAYYAKEIGLPVAKFICASNENNVLTDFFA
HHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEHHHHH
SGRYDKKRPFKVTTSPSMDILVSSNLERLVFHLLGNDAQKTAVLMKSLAETGQYQLADAD
CCCCCCCCCEEEECCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCH
QSILDLFAAGFATEQETAKEIKTIFESDHYVLDPHTAVASAVYRAYREQSGDSRPTIVAS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHCCCCCCEEEEE
TASPYKFPRVVVEAISGQQVASDFEALEQLADISGVVVPQAVNGLQEAPIRHSLLVTVAD
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHCCCHHHHHHHHHH
MQQAVEAYLGV
HHHHHHHHHCC
>Mature Secondary Structure
MYQSTRNAANKVTASQAILGGLADDGGLFTPTSMPVLDLDFEELKTKSYQEVTKLILSAF
CCCCHHHHHHHHHHHHHHHCCCCCCCCEECCCCCCEEECCHHHHHCCHHHHHHHHHHHHH
FDDYTEAELDACISSAYDDKFDTAVIAPLATLKDYHNLELFHGPTIAFKDMALSILPYLL
HHCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCEEEECCCHHHHHHHHHHHHHHH
TTAAKKQGVERKIAILTATSGDTGKAAMAGFADVPGTDIIVFYPKSGVSRIQELQMITQA
HHHHHHCCCCEEEEEEEECCCCCCCHHHCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHC
GHNTHVVAIEGNFDDAQTEVKRLFNDPDLRERLAAQQIQLSSANSMNIGRLIPQVVYYIY
CCCEEEEEEECCCCHHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHH
AYAQLVRHQRLEAGETINIVVPTGNFGNLLAAYYAKEIGLPVAKFICASNENNVLTDFFA
HHHHHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHHCCHHHHHHHCCCCCCEEHHHHH
SGRYDKKRPFKVTTSPSMDILVSSNLERLVFHLLGNDAQKTAVLMKSLAETGQYQLADAD
CCCCCCCCCEEEECCCCCEEEECCCHHHHHHHHHCCCHHHHHHHHHHHHHCCCEEECCCH
QSILDLFAAGFATEQETAKEIKTIFESDHYVLDPHTAVASAVYRAYREQSGDSRPTIVAS
HHHHHHHHHCCCCHHHHHHHHHHHHCCCCEEECCHHHHHHHHHHHHHHHCCCCCCEEEEE
TASPYKFPRVVVEAISGQQVASDFEALEQLADISGVVVPQAVNGLQEAPIRHSLLVTVAD
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCEEHHHHHHHHHHCCCHHHHHHHHHH
MQQAVEAYLGV
HHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]