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The map label for this gene is thiF [C]

Identifier: 195977200

GI number: 195977200

Start: 61312

End: 62433

Strand: Direct

Name: thiF [C]

Synonym: Sez_0048

Alternate gene names: 195977200

Gene position: 61312-62433 (Clockwise)

Preceding gene: 195977199

Following gene: 195977201

Centisome position: 3.03

GC content: 29.86

Gene sequence:

>1122_bases
ATGAATAATTTTTTTCCAAAGTGGAATCAGGATAGGATTGTATATTCATGGCAAAATGATAAATTAAGGATAGGAGCTGA
TGACAATGATGTTCTAGAAATCAAAGGAAACTCAATTTTTTGGTCAGATTTTATTTCTTGTTGTGACGGGAATAATAGTA
TCAAAGACATTCAAAAACTATTGAATATAAAATATAATATTTCAGAAGAAATAATTACAGAGTATTTAGATAGATTTTCA
AATCGAAATTTATTGGAAATATTGGATAATCCAATAAATCAATTACAGGATTATAGATCAATTGAGTCGTTAGAAACATA
TTATGCTAGTGAAGGAATTGGTGGGATTAAACTATTAAAAATGTTAGGGAATCTAAAGATTTCTATTCTGGGATGTGGTG
GTGGCGGCTCTCATATTGCATTACAATTAGCTCAATTAGGAGTGGGAAGAATTCATTTGGTGGATCACGATATCATTGAA
GAAAACAATATCAATAGACAATCCATGTTCACATTTAACGATGTCGGAAATTACAAAGTTGATTGTGTAGCCAAAAGTAT
TCAGCAAAGAAATTCTAAATGTTTGGTTACCAAAAGCACTTCTAAAATGATTAATGTACCTGATGTAAAAAAAGAAATAT
CTGAAAGCGATTGGGTATTCTGTTGCATGGATGAAGCTCCTTATATAGCACAGAGGATTGTAAATAGAGCATGTTATTTA
TTAAAAATTCCAAGTATATACTGTTTTAGCCAACGTAGTGCGGGGAAACTTTTGTTTTGTAATCCTAATATTGAAGATTC
TGGCTGTATTGATTGTTTGTTATCTGAACAAGATAATGAAAACTTTCAAAAGCTGGTGACAACGTTCACTACCTATAATA
GGAAATTGGTAACTGCAAATATCTTACCAAATATTTTATTATTGACATCGTGGGTGGTTAAACGATGGTTAGATTGTGTA
ATAGATAAAAATAGTAATGTATGGAATATGTTGTTGAGATTTGATTTTTATAAATATATGGAAGAAAAATTTCAATATTT
TTCGAAACAAGATAGTTGTCCAACATGTGCTAAGGGTCCTAGTACAAGTAAATTATGGGAGATTTTAAAAATAGATGAAT
AA

Upstream 100 bases:

>100_bases
AATGAACAAACCACAAATTGGTATTTTGTTTAATCACAAAGGCATGCAATGAGAATTTGCATGCCTTTGTAAAATATAGA
AAGGACAATAAGACAATAAA

Downstream 100 bases:

>100_bases
AAAAATTATTTTTAAAGGTGATCAAGATAGTAAATTTGCTTATTTTTCATTGATGTTTTCTGCAGGTACTGCCATTGAAA
ATACAGAAGAACTTGGTTTT

Product: HesA/MoeB/ThiF family protein

Products: ThiS-COSH; AMP; ThiS-COAMP; pyrophosphate [C]

Alternate protein names: HesA/MoeB/ThiF Family Protein; Molybdopterin Biosynthesis Protein MoeB; Molybdopterin Biosynthesis MoeB Protein; Molybdopterin Synthase Sulfurylase MoeB; Adenylyltransferase ThiF; ThiF Protein; Dinucleotide-Utilizing; Adenylyltransferase; ThiF Family Protein; Rhodanese/MoeB/ThiF Domain Protein; Dinucleotide-Utilizing Protein; Adenylyl Transferase; Molybdenum Cofactor Biosynthesis Protein; Dinucleotide-Utilizing S

Number of amino acids: Translated: 373; Mature: 373

Protein sequence:

>373_residues
MNNFFPKWNQDRIVYSWQNDKLRIGADDNDVLEIKGNSIFWSDFISCCDGNNSIKDIQKLLNIKYNISEEIITEYLDRFS
NRNLLEILDNPINQLQDYRSIESLETYYASEGIGGIKLLKMLGNLKISILGCGGGGSHIALQLAQLGVGRIHLVDHDIIE
ENNINRQSMFTFNDVGNYKVDCVAKSIQQRNSKCLVTKSTSKMINVPDVKKEISESDWVFCCMDEAPYIAQRIVNRACYL
LKIPSIYCFSQRSAGKLLFCNPNIEDSGCIDCLLSEQDNENFQKLVTTFTTYNRKLVTANILPNILLLTSWVVKRWLDCV
IDKNSNVWNMLLRFDFYKYMEEKFQYFSKQDSCPTCAKGPSTSKLWEILKIDE

Sequences:

>Translated_373_residues
MNNFFPKWNQDRIVYSWQNDKLRIGADDNDVLEIKGNSIFWSDFISCCDGNNSIKDIQKLLNIKYNISEEIITEYLDRFS
NRNLLEILDNPINQLQDYRSIESLETYYASEGIGGIKLLKMLGNLKISILGCGGGGSHIALQLAQLGVGRIHLVDHDIIE
ENNINRQSMFTFNDVGNYKVDCVAKSIQQRNSKCLVTKSTSKMINVPDVKKEISESDWVFCCMDEAPYIAQRIVNRACYL
LKIPSIYCFSQRSAGKLLFCNPNIEDSGCIDCLLSEQDNENFQKLVTTFTTYNRKLVTANILPNILLLTSWVVKRWLDCV
IDKNSNVWNMLLRFDFYKYMEEKFQYFSKQDSCPTCAKGPSTSKLWEILKIDE
>Mature_373_residues
MNNFFPKWNQDRIVYSWQNDKLRIGADDNDVLEIKGNSIFWSDFISCCDGNNSIKDIQKLLNIKYNISEEIITEYLDRFS
NRNLLEILDNPINQLQDYRSIESLETYYASEGIGGIKLLKMLGNLKISILGCGGGGSHIALQLAQLGVGRIHLVDHDIIE
ENNINRQSMFTFNDVGNYKVDCVAKSIQQRNSKCLVTKSTSKMINVPDVKKEISESDWVFCCMDEAPYIAQRIVNRACYL
LKIPSIYCFSQRSAGKLLFCNPNIEDSGCIDCLLSEQDNENFQKLVTTFTTYNRKLVTANILPNILLLTSWVVKRWLDCV
IDKNSNVWNMLLRFDFYKYMEEKFQYFSKQDSCPTCAKGPSTSKLWEILKIDE

Specific function: Catalyzes The Adenylation By ATP Of The Carboxyl- Terminal Glycine Of This. [C]

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI87082356, Length=233, Percent_Identity=24.8927038626609, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17540406, Length=250, Percent_Identity=24.4, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

NA

Pfam domain/function: NA

EC number: 2.7.7.- [C]

Molecular weight: Translated: 42958; Mature: 42958

Theoretical pI: Translated: 5.54; Mature: 5.54

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.0 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
4.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNFFPKWNQDRIVYSWQNDKLRIGADDNDVLEIKGNSIFWSDFISCCDGNNSIKDIQKL
CCCCCCCCCCCEEEEEECCCEEEECCCCCCEEEEECCEEEHHHHHHHCCCCCCHHHHHHH
LNIKYNISEEIITEYLDRFSNRNLLEILDNPINQLQDYRSIESLETYYASEGIGGIKLLK
HCCCCCCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
MLGNLKISILGCGGGGSHIALQLAQLGVGRIHLVDHDIIEENNINRQSMFTFNDVGNYKV
HHCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEECHHHCCCCCCHHHEEEEECCCCEEH
DCVAKSIQQRNSKCLVTKSTSKMINVPDVKKEISESDWVFCCMDEAPYIAQRIVNRACYL
HHHHHHHHHCCCEEEEEECCCCEECCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHCEEE
LKIPSIYCFSQRSAGKLLFCNPNIEDSGCIDCLLSEQDNENFQKLVTTFTTYNRKLVTAN
EECCCEEEECCCCCCEEEEECCCCCCCCHHEEEECCCCCHHHHHHHHHHHHCCCEEEEHH
ILPNILLLTSWVVKRWLDCVIDKNSNVWNMLLRFDFYKYMEEKFQYFSKQDSCPTCAKGP
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
STSKLWEILKIDE
CHHHHHHHHCCCC
>Mature Secondary Structure
MNNFFPKWNQDRIVYSWQNDKLRIGADDNDVLEIKGNSIFWSDFISCCDGNNSIKDIQKL
CCCCCCCCCCCEEEEEECCCEEEECCCCCCEEEEECCEEEHHHHHHHCCCCCCHHHHHHH
LNIKYNISEEIITEYLDRFSNRNLLEILDNPINQLQDYRSIESLETYYASEGIGGIKLLK
HCCCCCCCHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
MLGNLKISILGCGGGGSHIALQLAQLGVGRIHLVDHDIIEENNINRQSMFTFNDVGNYKV
HHCCCEEEEEEECCCCCHHHHHHHHCCCCEEEEEECHHHCCCCCCHHHEEEEECCCCEEH
DCVAKSIQQRNSKCLVTKSTSKMINVPDVKKEISESDWVFCCMDEAPYIAQRIVNRACYL
HHHHHHHHHCCCEEEEEECCCCEECCCHHHHHCCCCCEEEEEECCCCHHHHHHHHHCEEE
LKIPSIYCFSQRSAGKLLFCNPNIEDSGCIDCLLSEQDNENFQKLVTTFTTYNRKLVTAN
EECCCEEEECCCCCCEEEEECCCCCCCCHHEEEECCCCCHHHHHHHHHHHHCCCEEEEHH
ILPNILLLTSWVVKRWLDCVIDKNSNVWNMLLRFDFYKYMEEKFQYFSKQDSCPTCAKGP
HHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
STSKLWEILKIDE
CHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ThiS-COAMP; L-cysteine; ThiS protein; ATP [C]

Specific reaction: ThiS-COAMP + L-cysteine = ThiS-COSH + AMP ThiS protein + ATP = ThiS-COAMP + pyrophosphate [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA