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The map label for this gene is queA

Identifier: 195952881

GI number: 195952881

Start: 444947

End: 445978

Strand: Direct

Name: queA

Synonym: HY04AAS1_0506

Alternate gene names: 195952881

Gene position: 444947-445978 (Clockwise)

Preceding gene: 195952880

Following gene: 195952884

Centisome position: 28.53

GC content: 32.66

Gene sequence:

>1032_bases
ATGGATATATCACTGTTTGATTACGATTTACCAGAAGAGCTCATTGCCAAATACCCGGTAAATCCAAGACATAGCGCTAA
GCTGATGGTTTTAGATAGAAAAAGCTCCAATATAACTCACTCAACGTTTTGGCATATAGATGAGTTTTTGGAAGAAGGGG
ATTTATTGATATTCAACGACACCAAGGTATTACCGGCAAGACTTTTAGGAAAGAAAAAGGGGATAGAAAACTCAAATGTA
GAAATACTTCTTTTAAGACACTTAGAAAATGAAAAATGGGAAGCTCTTGTGGGGGGTAAAAATATAAAACCAGGGCTCGT
GATAGAAATATCTTATGATTTTGAAGCAATAGTAGAGTCTCAAATAGAAAAATCTAAGTTTTTGGTGCTTTTAAAAGCTA
AAAACCAAAATCAAATTGAGGCAATAAACAAATACGGCAAAATACCAATACCACCATATTTAGGAAGAGATGAAGAGCCA
ATAGATAAAGAATTTTATCAAACAGTATTTGCAAAAAAAGAATTTTCTGTGGCAGCCCCAACGGCATCGCTTCACTTTTC
TAATGAGCTTTTGGAAAAACTAAAGAAAAAAGTAAATATAGATTTTGTAACACTCCACGTATCATACGGAACTTTTAAAC
CAGTAACTGTGCAAAACATAGAAGAGCACAAAGTAGATGAAGAGTATATAGAAGTCTCAGAATCCCTTATAAAAAATATT
AAAAAAACAAAAAAAATGTGTAAAAGGGTAATAGCGGTTGGCACTACAGTTACAAGGGCATTAGAAACCGCTATCGATAA
ACCATATTTTGGTTTTACAGATCTTTATATAAAACCTGGATTTAAATTTAAAGTCCTAGATGGGTTTATTACAAACTTTC
ATCTTCCAAAATCATCACTTCTTATACTTGTAAGCGCTTTTTGTAATGAAGAAAACTTAGACGGTAGAGAGTTTATATTA
AAATCCTACAAAGAGGCTGTAAAACACAAATATAGATTTTACTCATACGGAGATGGCATGCTTATACTTTAG

Upstream 100 bases:

>100_bases
GGAAAAGGTATAGGTCTTGATTTTTATAGTGATTTTTCAAAGATATACAAAGGAAGTCTTCAAATTATCGACGATACCAC
AGATTTGTTGGAAATTTACA

Downstream 100 bases:

>100_bases
AAACTTAATTTTATGGTAAACTGCCGGGAAACCCTCCCGGCTATAAGTTTTGATTAGAAATAATTTACAGTGTTAGAACC
AGATACCAAAACAGGTTGAG

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MDISLFDYDLPEELIAKYPVNPRHSAKLMVLDRKSSNITHSTFWHIDEFLEEGDLLIFNDTKVLPARLLGKKKGIENSNV
EILLLRHLENEKWEALVGGKNIKPGLVIEISYDFEAIVESQIEKSKFLVLLKAKNQNQIEAINKYGKIPIPPYLGRDEEP
IDKEFYQTVFAKKEFSVAAPTASLHFSNELLEKLKKKVNIDFVTLHVSYGTFKPVTVQNIEEHKVDEEYIEVSESLIKNI
KKTKKMCKRVIAVGTTVTRALETAIDKPYFGFTDLYIKPGFKFKVLDGFITNFHLPKSSLLILVSAFCNEENLDGREFIL
KSYKEAVKHKYRFYSYGDGMLIL

Sequences:

>Translated_343_residues
MDISLFDYDLPEELIAKYPVNPRHSAKLMVLDRKSSNITHSTFWHIDEFLEEGDLLIFNDTKVLPARLLGKKKGIENSNV
EILLLRHLENEKWEALVGGKNIKPGLVIEISYDFEAIVESQIEKSKFLVLLKAKNQNQIEAINKYGKIPIPPYLGRDEEP
IDKEFYQTVFAKKEFSVAAPTASLHFSNELLEKLKKKVNIDFVTLHVSYGTFKPVTVQNIEEHKVDEEYIEVSESLIKNI
KKTKKMCKRVIAVGTTVTRALETAIDKPYFGFTDLYIKPGFKFKVLDGFITNFHLPKSSLLILVSAFCNEENLDGREFIL
KSYKEAVKHKYRFYSYGDGMLIL
>Mature_343_residues
MDISLFDYDLPEELIAKYPVNPRHSAKLMVLDRKSSNITHSTFWHIDEFLEEGDLLIFNDTKVLPARLLGKKKGIENSNV
EILLLRHLENEKWEALVGGKNIKPGLVIEISYDFEAIVESQIEKSKFLVLLKAKNQNQIEAINKYGKIPIPPYLGRDEEP
IDKEFYQTVFAKKEFSVAAPTASLHFSNELLEKLKKKVNIDFVTLHVSYGTFKPVTVQNIEEHKVDEEYIEVSESLIKNI
KKTKKMCKRVIAVGTTVTRALETAIDKPYFGFTDLYIKPGFKFKVLDGFITNFHLPKSSLLILVSAFCNEENLDGREFIL
KSYKEAVKHKYRFYSYGDGMLIL

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA)

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family

Homologues:

Organism=Escherichia coli, GI1786606, Length=352, Percent_Identity=42.0454545454545, Blast_Score=278, Evalue=4e-76,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): QUEA_HYDS0 (B4U7T2)

Other databases:

- EMBL:   CP001130
- RefSeq:   YP_002121171.1
- ProteinModelPortal:   B4U7T2
- SMR:   B4U7T2
- GeneID:   6743302
- GenomeReviews:   CP001130_GR
- KEGG:   hya:HY04AAS1_0506
- HOGENOM:   HBG286076
- OMA:   ITRNPAP
- ProtClustDB:   PRK00147
- GO:   GO:0005737
- HAMAP:   MF_00113
- InterPro:   IPR003699
- TIGRFAMs:   TIGR00113

Pfam domain/function: PF02547 Queuosine_synth; SSF111337 Queuosine_synth

EC number: 5.-.-.- [C]

Molecular weight: Translated: 39462; Mature: 39462

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDISLFDYDLPEELIAKYPVNPRHSAKLMVLDRKSSNITHSTFWHIDEFLEEGDLLIFND
CCCEEECCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEC
TKVLPARLLGKKKGIENSNVEILLLRHLENEKWEALVGGKNIKPGLVIEISYDFEAIVES
CCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCEEEEEECCHHHHHHH
QIEKSKFLVLLKAKNQNQIEAINKYGKIPIPPYLGRDEEPIDKEFYQTVFAKKEFSVAAP
HHCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECC
TASLHFSNELLEKLKKKVNIDFVTLHVSYGTFKPVTVQNIEEHKVDEEYIEVSESLIKNI
CHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEECCCHHHCCCHHHHHHHHHHHHHH
KKTKKMCKRVIAVGTTVTRALETAIDKPYFGFTDLYIKPGFKFKVLDGFITNFHLPKSSL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECHHHHHCCCCCHHH
LILVSAFCNEENLDGREFILKSYKEAVKHKYRFYSYGDGMLIL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCEEEC
>Mature Secondary Structure
MDISLFDYDLPEELIAKYPVNPRHSAKLMVLDRKSSNITHSTFWHIDEFLEEGDLLIFND
CCCEEECCCCCHHHHHHCCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEC
TKVLPARLLGKKKGIENSNVEILLLRHLENEKWEALVGGKNIKPGLVIEISYDFEAIVES
CCHHHHHHHHHHCCCCCCCEEEEEEEECCCCCEEEEECCCCCCCCEEEEEECCHHHHHHH
QIEKSKFLVLLKAKNQNQIEAINKYGKIPIPPYLGRDEEPIDKEFYQTVFAKKEFSVAAP
HHCCCEEEEEEECCCCCHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEECC
TASLHFSNELLEKLKKKVNIDFVTLHVSYGTFKPVTVQNIEEHKVDEEYIEVSESLIKNI
CHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCCCEEECCCHHHCCCHHHHHHHHHHHHHH
KKTKKMCKRVIAVGTTVTRALETAIDKPYFGFTDLYIKPGFKFKVLDGFITNFHLPKSSL
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEEECHHHHHCCCCCHHH
LILVSAFCNEENLDGREFILKSYKEAVKHKYRFYSYGDGMLIL
HHHHHHHCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA