Click here to switch to the map view.

The map label for this gene is dut

Identifier: 195952524

GI number: 195952524

Start: 129506

End: 129940

Strand: Direct

Name: dut

Synonym: HY04AAS1_0144

Alternate gene names: 195952524

Gene position: 129506-129940 (Clockwise)

Preceding gene: 195952523

Following gene: 195952525

Centisome position: 8.3

GC content: 37.7

Gene sequence:

>435_bases
ATGAAGCTTTTAGTAAAAAAACTAAGAGAAGATGCTGCACTTCCTTTTTATGCTACCTCTGGTGCTTCTGGACTCGATCT
TTTTTGCGTTGATGATGTAGTAGTGATAAGACCTTTTGAAAGAGTGTTGGTGTCTACTGGAATAGCCATAGAGCTTCCAG
AAGGCTATGAAGCTCAAATAAGACCAAGAAGCGGATTGGCTCTTAAAAAGGGTGTAACTGTGTTAAACAGCCCAGGTACT
ATAGACCATGACTATAGAGGTGAAATAAAAGTCATACTTATAAATTTATCGGACAAGGAAGTAATTATTGAAAAAGGTGA
AAGGATAGCTCAGATGGTTATAGTTCCTGTTTTAAAGGTAGAAGTTGTAGAGGCCAAAGAACTCTCTAATACACAAAGAC
AAGATGGTGGTTTTGGTTCCACAGGTATGAAATGA

Upstream 100 bases:

>100_bases
TAGGTTAAACCTCTGTAGTCCAGACTATCAAAAGCCAGAAAACCAAGAAAGACCACGAAAAGAACAACTTAATAGGAAAC
CCCATCACAGAAAATAGTTT

Downstream 100 bases:

>100_bases
TAGATGTTCTTTCTAAATTATCGATAAAGGAAAATCTAACAAAAGAAGATATAAGACAAATGTTAGAATCCATTGCTTCA
AACGAAGCCACAGAAGCTCA

Product: deoxyuridine 5'-triphosphate nucleotidohydrolase Dut

Products: NA

Alternate protein names: dUTPase; dUTP pyrophosphatase

Number of amino acids: Translated: 144; Mature: 144

Protein sequence:

>144_residues
MKLLVKKLREDAALPFYATSGASGLDLFCVDDVVVIRPFERVLVSTGIAIELPEGYEAQIRPRSGLALKKGVTVLNSPGT
IDHDYRGEIKVILINLSDKEVIIEKGERIAQMVIVPVLKVEVVEAKELSNTQRQDGGFGSTGMK

Sequences:

>Translated_144_residues
MKLLVKKLREDAALPFYATSGASGLDLFCVDDVVVIRPFERVLVSTGIAIELPEGYEAQIRPRSGLALKKGVTVLNSPGT
IDHDYRGEIKVILINLSDKEVIIEKGERIAQMVIVPVLKVEVVEAKELSNTQRQDGGFGSTGMK
>Mature_144_residues
MKLLVKKLREDAALPFYATSGASGLDLFCVDDVVVIRPFERVLVSTGIAIELPEGYEAQIRPRSGLALKKGVTVLNSPGT
IDHDYRGEIKVILINLSDKEVIIEKGERIAQMVIVPVLKVEVVEAKELSNTQRQDGGFGSTGMK

Specific function: This enzyme is involved in nucleotide metabolism:it produces dUMP, the immediate precursor of thymidine nucleotides and it decreases the intracellular concentration of dUTP so that uracil cannot be incorporated into DNA

COG id: COG0756

COG function: function code F; dUTPase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dUTPase family

Homologues:

Organism=Homo sapiens, GI70906444, Length=142, Percent_Identity=40.1408450704225, Blast_Score=105, Evalue=1e-23,
Organism=Homo sapiens, GI4503423, Length=142, Percent_Identity=40.1408450704225, Blast_Score=105, Evalue=1e-23,
Organism=Homo sapiens, GI70906441, Length=142, Percent_Identity=40.1408450704225, Blast_Score=105, Evalue=2e-23,
Organism=Escherichia coli, GI1790071, Length=144, Percent_Identity=40.2777777777778, Blast_Score=108, Evalue=9e-26,
Organism=Caenorhabditis elegans, GI71988561, Length=138, Percent_Identity=44.2028985507246, Blast_Score=115, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6319729, Length=143, Percent_Identity=37.0629370629371, Blast_Score=93, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24583610, Length=143, Percent_Identity=38.4615384615385, Blast_Score=96, Evalue=1e-20,
Organism=Drosophila melanogaster, GI19921126, Length=143, Percent_Identity=38.4615384615385, Blast_Score=96, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DUT_HYDS0 (B4U6R8)

Other databases:

- EMBL:   CP001130
- RefSeq:   YP_002120814.1
- ProteinModelPortal:   B4U6R8
- SMR:   B4U6R8
- GeneID:   6742931
- GenomeReviews:   CP001130_GR
- KEGG:   hya:HY04AAS1_0144
- HOGENOM:   HBG436079
- OMA:   HGIALVN
- HAMAP:   MF_00116
- InterPro:   IPR008180
- InterPro:   IPR008181
- TIGRFAMs:   TIGR00576

Pfam domain/function: PF00692 dUTPase

EC number: =3.6.1.23

Molecular weight: Translated: 15723; Mature: 15723

Theoretical pI: Translated: 5.97; Mature: 5.97

Prosite motif: NA

Important sites: BINDING 76-76 BINDING 90-90

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLLVKKLREDAALPFYATSGASGLDLFCVDDVVVIRPFERVLVSTGIAIELPEGYEAQI
CHHHHHHHHHCCCCCEEECCCCCCCEEEEECCEEEECCHHHHHHHCCCEEECCCCCCEEE
RPRSGLALKKGVTVLNSPGTIDHDYRGEIKVILINLSDKEVIIEKGERIAQMVIVPVLKV
CCCCCCEEECCEEEECCCCCCCCCCCCCEEEEEEECCCCEEEECCCHHHHHHHHHHHHEE
EVVEAKELSNTQRQDGGFGSTGMK
EEECHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKLLVKKLREDAALPFYATSGASGLDLFCVDDVVVIRPFERVLVSTGIAIELPEGYEAQI
CHHHHHHHHHCCCCCEEECCCCCCCEEEEECCEEEECCHHHHHHHCCCEEECCCCCCEEE
RPRSGLALKKGVTVLNSPGTIDHDYRGEIKVILINLSDKEVIIEKGERIAQMVIVPVLKV
CCCCCCEEECCEEEECCCCCCCCCCCCCEEEEEEECCCCEEEECCCHHHHHHHHHHHHEE
EVVEAKELSNTQRQDGGFGSTGMK
EEECHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA