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The map label for this gene is dnaA [H]

Identifier: 195952381

GI number: 195952381

Start: 265

End: 1599

Strand: Direct

Name: dnaA [H]

Synonym: HY04AAS1_0001

Alternate gene names: 195952381

Gene position: 265-1599 (Clockwise)

Preceding gene: NA

Following gene: 195952384

Centisome position: 0.02

GC content: 33.93

Gene sequence:

>1335_bases
ATGGCCCTTGTTAAAAGCTTAAAACCAATAGCTATTGAAGGAAAAAGCCTTGTATTGGAATGCCCCACAGAGCAATCAAA
AAACTGGATACATCAAACCCTTGGTAACATACTAAGAGATGAAGCTTATAAGAAAGGCTTTTCCATAAAACTTGTGATAA
AAGAGCAAGATAATCCTAATATAGACATAAGTTATTCAACTTACAGAGATAGTGTTGATAGGCAAACTGCCGTAGATAAA
GCGCCTTTTAGCATGTCTATACCTTCATACCCTAACGCTGAAACTAAGTTGAACCCCTCTGATGTAAAAAATAAAACCGC
TTTTAAAGAAGGTATATCTTCTAAGTATACATTTGATAACTTTATAGTGGGTAAACAAAACGAAGTGGCTTATAGGGCAT
CTTTAGAAGTGGCTACAGATGAAAATTCTATATATAACCCCCTTTTTATATACGGGAAGGTAGGAAGCGGTAAAACGCAT
CTTTTGCAAGCTATAGGCAACAGAGCTTACTCTCTTGGAAAAAGCGTTATATATACATCCATGAACGATTTTACAGAGGA
AATGGTTTATTATTTAAAGGCTGGCAATATAATAAGCTTTAGAGAAAAATACAAAGGCGTAGATATCTTACTCATAGATG
ATATACAGTTTTTGTCTGGTAAAGAACGTACGCAGATGGAGCTTTTTAACATATTTAACTATCTTTTTCAATACAACAAA
CATATAGTATTTGCAAGCGATAAGCATCCAAGAGACATAAAAGATATCTCCGAAAGGCTTGTAAGTAGGTTTGAAGGTGG
TCTTTTGGTGGAAACCAGCATAGATGATCAAATAAAGCTTTCTATTATAAAACAAAAGATTCAGATTTATGGACTAAACG
TAGACGAAAGGCTTATATCCTATATAAAAGACAATACCACCAACAACGCTAGAGAGATTGAAGGTCTTGTGCTTCAGATA
AAGGCAAAAGGCGTTTCTATATTAGAAGCTTACAACTCTGCCACGCATTACAACTCTACCACAGTTACCAACACACCAAA
AGAAATAAGCATAGACAAGATTAAAAAAGCTGTAGCAAACTATTTTAACATTGGTATGGATATCTTTAATAAAAGCTATA
AAAACAAAAAGTACGCTACAGCAAAACATATAGCTATCTTTTTATCAAGAGAGTTTACGGGCTTTTCTCTTGCTGAGATA
GCAAAGGCTTTTAACATAAAGTCCCATTCAAGCGTTAACCATTCTATCAAAAAGATAGAAAAACAGATAAAAGAGGACAA
AACGATTCAGTACTCTGTATCCTCTTTAAGAGAGATTATAAGAAAATTAGATTGA

Upstream 100 bases:

>100_bases
TGTTTTAGGATTTTCAAATCAAAAAAATACCAATAAAGACTGCAAAGGCATATGGGAAAACATACTTGTTGGTATAAGCA
AGAAAATTGACAAACCTACA

Downstream 100 bases:

>100_bases
TTATCTTATGTTTCTGTATATAATGTATAAGCCTACAAGTATAACCGCTATAGCGTAAAAGGTTTTTAGTTTTTTTGCGT
TTAATCTAGTTGCCAGTTTC

Product: chromosomal replication initiator protein DnaA

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 444; Mature: 443

Protein sequence:

>444_residues
MALVKSLKPIAIEGKSLVLECPTEQSKNWIHQTLGNILRDEAYKKGFSIKLVIKEQDNPNIDISYSTYRDSVDRQTAVDK
APFSMSIPSYPNAETKLNPSDVKNKTAFKEGISSKYTFDNFIVGKQNEVAYRASLEVATDENSIYNPLFIYGKVGSGKTH
LLQAIGNRAYSLGKSVIYTSMNDFTEEMVYYLKAGNIISFREKYKGVDILLIDDIQFLSGKERTQMELFNIFNYLFQYNK
HIVFASDKHPRDIKDISERLVSRFEGGLLVETSIDDQIKLSIIKQKIQIYGLNVDERLISYIKDNTTNNAREIEGLVLQI
KAKGVSILEAYNSATHYNSTTVTNTPKEISIDKIKKAVANYFNIGMDIFNKSYKNKKYATAKHIAIFLSREFTGFSLAEI
AKAFNIKSHSSVNHSIKKIEKQIKEDKTIQYSVSSLREIIRKLD

Sequences:

>Translated_444_residues
MALVKSLKPIAIEGKSLVLECPTEQSKNWIHQTLGNILRDEAYKKGFSIKLVIKEQDNPNIDISYSTYRDSVDRQTAVDK
APFSMSIPSYPNAETKLNPSDVKNKTAFKEGISSKYTFDNFIVGKQNEVAYRASLEVATDENSIYNPLFIYGKVGSGKTH
LLQAIGNRAYSLGKSVIYTSMNDFTEEMVYYLKAGNIISFREKYKGVDILLIDDIQFLSGKERTQMELFNIFNYLFQYNK
HIVFASDKHPRDIKDISERLVSRFEGGLLVETSIDDQIKLSIIKQKIQIYGLNVDERLISYIKDNTTNNAREIEGLVLQI
KAKGVSILEAYNSATHYNSTTVTNTPKEISIDKIKKAVANYFNIGMDIFNKSYKNKKYATAKHIAIFLSREFTGFSLAEI
AKAFNIKSHSSVNHSIKKIEKQIKEDKTIQYSVSSLREIIRKLD
>Mature_443_residues
ALVKSLKPIAIEGKSLVLECPTEQSKNWIHQTLGNILRDEAYKKGFSIKLVIKEQDNPNIDISYSTYRDSVDRQTAVDKA
PFSMSIPSYPNAETKLNPSDVKNKTAFKEGISSKYTFDNFIVGKQNEVAYRASLEVATDENSIYNPLFIYGKVGSGKTHL
LQAIGNRAYSLGKSVIYTSMNDFTEEMVYYLKAGNIISFREKYKGVDILLIDDIQFLSGKERTQMELFNIFNYLFQYNKH
IVFASDKHPRDIKDISERLVSRFEGGLLVETSIDDQIKLSIIKQKIQIYGLNVDERLISYIKDNTTNNAREIEGLVLQIK
AKGVSILEAYNSATHYNSTTVTNTPKEISIDKIKKAVANYFNIGMDIFNKSYKNKKYATAKHIAIFLSREFTGFSLAEIA
KAFNIKSHSSVNHSIKKIEKQIKEDKTIQYSVSSLREIIRKLD

Specific function: Plays an important role in the initiation and regulation of chromosomal replication. Binds to the origin of replication; it binds specifically double-stranded DNA at a 9 bp consensus (dnaA box):5'-TTATC[CA]A[CA]A-3'. DnaA binds to ATP and to acidic phosph

COG id: COG0593

COG function: function code L; ATPase involved in DNA replication initiation

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dnaA family [H]

Homologues:

Organism=Escherichia coli, GI2367267, Length=337, Percent_Identity=34.4213649851632, Blast_Score=199, Evalue=2e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR001957
- InterPro:   IPR020591
- InterPro:   IPR018312
- InterPro:   IPR013159
- InterPro:   IPR013317
- InterPro:   IPR010921 [H]

Pfam domain/function: PF00308 Bac_DnaA; PF08299 Bac_DnaA_C [H]

EC number: NA

Molecular weight: Translated: 50521; Mature: 50390

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS01008 DNAA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALVKSLKPIAIEGKSLVLECPTEQSKNWIHQTLGNILRDEAYKKGFSIKLVIKEQDNPN
CCCHHCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
IDISYSTYRDSVDRQTAVDKAPFSMSIPSYPNAETKLNPSDVKNKTAFKEGISSKYTFDN
EEEEEHHHHHCCHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECC
FIVGKQNEVAYRASLEVATDENSIYNPLFIYGKVGSGKTHLLQAIGNRAYSLGKSVIYTS
EEECCCCCEEEEEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHEEHH
MNDFTEEMVYYLKAGNIISFREKYKGVDILLIDDIQFLSGKERTQMELFNIFNYLFQYNK
HHHHHHHHHHHHHCCCEEEHHHHHCCCEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHCC
HIVFASDKHPRDIKDISERLVSRFEGGLLVETSIDDQIKLSIIKQKIQIYGLNVDERLIS
EEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHEEECCHHHHHHH
YIKDNTTNNAREIEGLVLQIKAKGVSILEAYNSATHYNSTTVTNTPKEISIDKIKKAVAN
HHHCCCCCCHHHHCEEEEEEEECCCHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHHH
YFNIGMDIFNKSYKNKKYATAKHIAIFLSREFTGFSLAEIAKAFNIKSHSSVNHSIKKIE
HHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
KQIKEDKTIQYSVSSLREIIRKLD
HHHHHCCEEEHHHHHHHHHHHHCC
>Mature Secondary Structure 
ALVKSLKPIAIEGKSLVLECPTEQSKNWIHQTLGNILRDEAYKKGFSIKLVIKEQDNPN
CCHHCCCCEEECCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCC
IDISYSTYRDSVDRQTAVDKAPFSMSIPSYPNAETKLNPSDVKNKTAFKEGISSKYTFDN
EEEEEHHHHHCCHHHHHHCCCCCEEECCCCCCCCCCCCHHHHHHHHHHHHCCCCCCEECC
FIVGKQNEVAYRASLEVATDENSIYNPLFIYGKVGSGKTHLLQAIGNRAYSLGKSVIYTS
EEECCCCCEEEEEEEEEEECCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHCCHHEEHH
MNDFTEEMVYYLKAGNIISFREKYKGVDILLIDDIQFLSGKERTQMELFNIFNYLFQYNK
HHHHHHHHHHHHHCCCEEEHHHHHCCCEEEEECCCHHHCCCCHHHHHHHHHHHHHHHHCC
HIVFASDKHPRDIKDISERLVSRFEGGLLVETSIDDQIKLSIIKQKIQIYGLNVDERLIS
EEEEECCCCCCHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHEEECCHHHHHHH
YIKDNTTNNAREIEGLVLQIKAKGVSILEAYNSATHYNSTTVTNTPKEISIDKIKKAVAN
HHHCCCCCCHHHHCEEEEEEEECCCHHHHHHCCCCCCCCCEECCCCCCCCHHHHHHHHHH
YFNIGMDIFNKSYKNKKYATAKHIAIFLSREFTGFSLAEIAKAFNIKSHSSVNHSIKKIE
HHHHHHHHHHHHHCCCCCCCHHHEEEEEECCCCCCCHHHHHHHHCCCCCCHHHHHHHHHH
KQIKEDKTIQYSVSSLREIIRKLD
HHHHHCCEEEHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9537320; 12234917 [H]