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The map label for this gene is ycdT [H]

Identifier: 194738368

GI number: 194738368

Start: 4665458

End: 4666372

Strand: Reverse

Name: ycdT [H]

Synonym: SeSA_A4804

Alternate gene names: 194738368

Gene position: 4666372-4665458 (Counterclockwise)

Preceding gene: 194736271

Following gene: 194734691

Centisome position: 99.09

GC content: 50.82

Gene sequence:

>915_bases
ATGGTCCTGAGCGTATTACTGTTGATATGGCATGGGAAATATGGACAAAAAAGAATTAATCTCCCCTTTATCTCCCTACT
CTTTGGGGGAATATGGGCGGCGCATATTGCGCTAAAGTATCCGGCATTAGGCCATTATGATTTCTCTTTTTTACTGATTT
CCCTGCTTAGCGTTCTGTTTATCGGCTCGATAGCCTTTGCCGCGAATATCGTCGCGTTTACGCTCTATTCGCTACCGCCA
GTCGCGGTCTGCCTGTGGCTGAACGGTAATGAACAAGGTTTGCGAATTCTCTATTTGCTGGCCCTGCCAATGGTGGGAAT
CGCTATTCAACATGTTATCCAGAAACGCTACGATAACTTTGCCCAGCAGCTCATGTTTAAGCTGCTGGCCGAGCGGGAAA
CCCTCAATGGCCTGAGTATGCTTGATCCATTAACCGGCTTATATAACCGCCGTGGCCTGCAAAATCGGCTGGATACGTTA
CAGGCGCCGGGCAGCCATGAACACTACGTCCTGCTATTGGATATCGATCATTTCAAAGCGTATAACGATCACTACGGTCA
TATGATGGGCGATCAGGCGTTAATCCGCGTCTCAGCGGCAATTCGGGACGCCGTGCGATCGCGCGATGTCGTCTGCCGTT
TTGGCGGGGAAGAATTTCTGGTGTTGCTGACCACCGCCGAACCACAGCAAGCCCGCGCTACCGCAGAACGTATCCGACAG
GAAGTATACGATCTTAAAATCCCGCATATGTTTAACGAAAGCGTCGCCACCAATGTCACTGTCAGTATCGGCATTGCGCC
CCTGGCGGATCGCAATATCGGAGACGCTATCGAAAAAGCGGATAAAGCGCTCTACGAAGCCAAGCATCTTGGACGTAACC
ACATTCTGGTCAGCGACGACATGCGCGCCCTATGA

Upstream 100 bases:

>100_bases
TTTACGTCTTTTCTTGTTTCTCAACGCCGTGTCGGCGCTATTTACTCTCGTTTTCCCCCTTTATCAAATCAATGTATTTT
GCACGCCAATGATTGGGATA

Downstream 100 bases:

>100_bases
ATCCTGCTGGCGTAATCGTGCTACGCCAACAGCGTTAACCATAAACCGCTTGCCATCGTTTATCGCTATCGATAGGATTA
GCAATCATTATCATTTAGAT

Product: diguanylate cyclase

Products: NA

Alternate protein names: DGC [H]

Number of amino acids: Translated: 304; Mature: 304

Protein sequence:

>304_residues
MVLSVLLLIWHGKYGQKRINLPFISLLFGGIWAAHIALKYPALGHYDFSFLLISLLSVLFIGSIAFAANIVAFTLYSLPP
VAVCLWLNGNEQGLRILYLLALPMVGIAIQHVIQKRYDNFAQQLMFKLLAERETLNGLSMLDPLTGLYNRRGLQNRLDTL
QAPGSHEHYVLLLDIDHFKAYNDHYGHMMGDQALIRVSAAIRDAVRSRDVVCRFGGEEFLVLLTTAEPQQARATAERIRQ
EVYDLKIPHMFNESVATNVTVSIGIAPLADRNIGDAIEKADKALYEAKHLGRNHILVSDDMRAL

Sequences:

>Translated_304_residues
MVLSVLLLIWHGKYGQKRINLPFISLLFGGIWAAHIALKYPALGHYDFSFLLISLLSVLFIGSIAFAANIVAFTLYSLPP
VAVCLWLNGNEQGLRILYLLALPMVGIAIQHVIQKRYDNFAQQLMFKLLAERETLNGLSMLDPLTGLYNRRGLQNRLDTL
QAPGSHEHYVLLLDIDHFKAYNDHYGHMMGDQALIRVSAAIRDAVRSRDVVCRFGGEEFLVLLTTAEPQQARATAERIRQ
EVYDLKIPHMFNESVATNVTVSIGIAPLADRNIGDAIEKADKALYEAKHLGRNHILVSDDMRAL
>Mature_304_residues
MVLSVLLLIWHGKYGQKRINLPFISLLFGGIWAAHIALKYPALGHYDFSFLLISLLSVLFIGSIAFAANIVAFTLYSLPP
VAVCLWLNGNEQGLRILYLLALPMVGIAIQHVIQKRYDNFAQQLMFKLLAERETLNGLSMLDPLTGLYNRRGLQNRLDTL
QAPGSHEHYVLLLDIDHFKAYNDHYGHMMGDQALIRVSAAIRDAVRSRDVVCRFGGEEFLVLLTTAEPQQARATAERIRQ
EVYDLKIPHMFNESVATNVTVSIGIAPLADRNIGDAIEKADKALYEAKHLGRNHILVSDDMRAL

Specific function: A probable diguanylate cyclase, overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di- GMP i

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 GGDEF domain [H]

Homologues:

Organism=Escherichia coli, GI1787262, Length=161, Percent_Identity=40.9937888198758, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1786584, Length=182, Percent_Identity=32.4175824175824, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1788085, Length=177, Percent_Identity=34.4632768361582, Blast_Score=101, Evalue=6e-23,
Organism=Escherichia coli, GI145693134, Length=170, Percent_Identity=41.1764705882353, Blast_Score=99, Evalue=4e-22,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=35.7575757575758, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1787816, Length=207, Percent_Identity=31.8840579710145, Blast_Score=92, Evalue=6e-20,
Organism=Escherichia coli, GI87081881, Length=175, Percent_Identity=35.4285714285714, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1787541, Length=168, Percent_Identity=35.7142857142857, Blast_Score=88, Evalue=7e-19,
Organism=Escherichia coli, GI1787802, Length=161, Percent_Identity=35.4037267080745, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1788381, Length=167, Percent_Identity=34.1317365269461, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI87081974, Length=153, Percent_Identity=32.0261437908497, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1788956, Length=175, Percent_Identity=30.2857142857143, Blast_Score=73, Evalue=3e-14,
Organism=Escherichia coli, GI1787056, Length=162, Percent_Identity=29.6296296296296, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI87081977, Length=107, Percent_Identity=37.3831775700935, Blast_Score=67, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR000160 [H]

Pfam domain/function: PF00990 GGDEF [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 34104; Mature: 34104

Theoretical pI: Translated: 7.82; Mature: 7.82

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLSVLLLIWHGKYGQKRINLPFISLLFGGIWAAHIALKYPALGHYDFSFLLISLLSVLF
CHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
IGSIAFAANIVAFTLYSLPPVAVCLWLNGNEQGLRILYLLALPMVGIAIQHVIQKRYDNF
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQQLMFKLLAERETLNGLSMLDPLTGLYNRRGLQNRLDTLQAPGSHEHYVLLLDIDHFKA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEHHHHH
YNDHYGHMMGDQALIRVSAAIRDAVRSRDVVCRFGGEEFLVLLTTAEPQQARATAERIRQ
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHH
EVYDLKIPHMFNESVATNVTVSIGIAPLADRNIGDAIEKADKALYEAKHLGRNHILVSDD
HHHHCCCCCHHCCHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCC
MRAL
HHCC
>Mature Secondary Structure
MVLSVLLLIWHGKYGQKRINLPFISLLFGGIWAAHIALKYPALGHYDFSFLLISLLSVLF
CHHHHHHHHHCCCCCCCEECCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
IGSIAFAANIVAFTLYSLPPVAVCLWLNGNEQGLRILYLLALPMVGIAIQHVIQKRYDNF
HHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQQLMFKLLAERETLNGLSMLDPLTGLYNRRGLQNRLDTLQAPGSHEHYVLLLDIDHFKA
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCEEEEEEEHHHHH
YNDHYGHMMGDQALIRVSAAIRDAVRSRDVVCRFGGEEFLVLLTTAEPQQARATAERIRQ
CCCCCCCCCCCHHHHHHHHHHHHHHHCCCEEEEECCCEEEEEEECCCCHHHHHHHHHHHH
EVYDLKIPHMFNESVATNVTVSIGIAPLADRNIGDAIEKADKALYEAKHLGRNHILVSDD
HHHHCCCCCHHCCHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCC
MRAL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]