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The map label for this gene is purH [H]

Identifier: 194738357

GI number: 194738357

Start: 4258468

End: 4260057

Strand: Reverse

Name: purH [H]

Synonym: SeSA_A4387

Alternate gene names: 194738357

Gene position: 4260057-4258468 (Counterclockwise)

Preceding gene: 194737125

Following gene: 194734737

Centisome position: 90.46

GC content: 56.54

Gene sequence:

>1590_bases
ATGCAACAACGTCGTCCAGTCCGCCGCGCTCTGCTCAGTGTTTCTGACAAGGCCGGTATCATCGAATTCGCCCAGGCACT
TTCCGCACGCGGTGTGGAACTGCTGTCTACGGGGGGCACCGCCCGCCTGTTAGCAGAAAAAGGCCTGCCGGTGACCGAAG
TTTCCGATTACACCGGTTTCCCGGAAATGATGGATGGACGCGTAAAGACCCTGCATCCAAAAGTACACGGCGGCATCCTC
GGCCGTCGCGGCCAGGACGATGCCATTATGGAACAGCACCACATCGCCCCTATCGATATGGTTGTCGTTAACCTGTATCC
GTTCGCCGAAACCGTTGCACGCGTTGGTTGCTCGCTGGAAGATGCAGTAGAGAACATCGATATCGGCGGCCCGACCATGG
TGCGCTCCGCCGCGAAGAACCATAAAGACGTGGCCATCGTGGTGAAGAGCAGCGACTACGACGCCATTATTAAAGAGATG
GACGCTAACGAAGGTTCTTTGACCCTCGACACCCGTTTCGATCTCGCGATTAAAGCCTTCGAACACACCGCCGCCTACGA
CAGCATGATCGCTAACTACTTCGGCAGCATGGTTCCGGCCTACCACGGTGAAAGCAAAGAAGCCGCCGGTCGCTTCCCGC
GTACGCTGAACCTGAACTTCATTAAGAAGCAGGATATGCGCTACGGCGAGAACAGCCACCAGCAAGCTGCCTTCTATATA
GAAGAGAATGTGAAAGAAGCGTCCGTTGCCACCGCACAACAGGTGCAGGGCAAAGCGCTCTCTTATAACAACATCGCTGA
TACCGACGCGGCGCTGGAGTGTGTGAAAGAGTTCAACGAGCCAGCCTGCGTAATCGTCAAGCACGCTAACCCGTGCGGCG
TGGCGGTAAGTACCTCTATTCTCGACGCTTACGATCGCGCCTATAAAACCGACCCGACCTCCGCGTTCGGCGGCATTATC
GCCTTTAACCGTGAGCTGGATGCGGAAACCGCACAGGCCATCATTTCTCGTCAGTTTGTCGAAGTGATCATCGCCCCATC
CGCAAGCGAAGAAGCGCTGAAAATCACCGCTGCCAAGCAGAACGTCCGTGTTCTGACCTGCGGCCAATGGGCAAGCCGCG
TTCCGGGCCTGGATTTCAAACGCGTTAACGGTGGCCTGCTGGTTCAGGACAGGGATCTGGGTATGGTGAGTGAAGCTGAA
CTGCGCGTGGTGTCCAAACGCCAGCCGACCGAGCAGGAGCTGCGTGACGCGCTGTTCTGCTGGAAGGTAGCCAAGTTCGT
GAAATCCAACGCTATTGTGTATGCCAAAGAGAACATGACCATCGGTATAGGCGCAGGCCAGATGAGCCGCGTGTACTCCG
CCAAAATCGCCGGGATTAAAGCCGCTGATGAAGGTCTGGAAGTGAAAGGCTCAGCCATGGCTTCCGACGCGTTCTTCCCG
TTCCGCGATGGTATTGATGCCGCTGCCGCTGTCGGCGTGAGCTGCGTTATCCAGCCAGGCGGTTCTATCCGTGATGATGA
AGTCATTGCCGCTGCCGATGAACACGGTATTGCGATGATCTTCACCGACATGCGCCACTTCCGCCATTAA

Upstream 100 bases:

>100_bases
TCGCCGCAAAATGAGAATGCCCCATAAGGGGCGTTAGCTGAGTTTTTTACGAAAAATTCAGCTAACGCTCTCTGTAATCG
TCAAATCCAGGGGATTTACC

Downstream 100 bases:

>100_bases
TTCAGGGAGCAGACGATGAAAGTTTTAGTCATTGGTAACGGCGGGCGCGAACACGCGCTGGCCTGGAAAGCCGCACAGTC
GCCGCTGGTTGATACCGTTT

Product: bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Products: NA

Alternate protein names: Phosphoribosylaminoimidazolecarboxamide formyltransferase; AICAR transformylase; IMP cyclohydrolase; ATIC; IMP synthase; Inosinicase [H]

Number of amino acids: Translated: 529; Mature: 529

Protein sequence:

>529_residues
MQQRRPVRRALLSVSDKAGIIEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDAIMEQHHIAPIDMVVVNLYPFAETVARVGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
DANEGSLTLDTRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATAQQVQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVAVSTSILDAYDRAYKTDPTSAFGGII
AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWASRVPGLDFKRVNGGLLVQDRDLGMVSEAE
LRVVSKRQPTEQELRDALFCWKVAKFVKSNAIVYAKENMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSAMASDAFFP
FRDGIDAAAAVGVSCVIQPGGSIRDDEVIAAADEHGIAMIFTDMRHFRH

Sequences:

>Translated_529_residues
MQQRRPVRRALLSVSDKAGIIEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDAIMEQHHIAPIDMVVVNLYPFAETVARVGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
DANEGSLTLDTRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATAQQVQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVAVSTSILDAYDRAYKTDPTSAFGGII
AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWASRVPGLDFKRVNGGLLVQDRDLGMVSEAE
LRVVSKRQPTEQELRDALFCWKVAKFVKSNAIVYAKENMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSAMASDAFFP
FRDGIDAAAAVGVSCVIQPGGSIRDDEVIAAADEHGIAMIFTDMRHFRH
>Mature_529_residues
MQQRRPVRRALLSVSDKAGIIEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGFPEMMDGRVKTLHPKVHGGIL
GRRGQDDAIMEQHHIAPIDMVVVNLYPFAETVARVGCSLEDAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEM
DANEGSLTLDTRFDLAIKAFEHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
EENVKEASVATAQQVQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVAVSTSILDAYDRAYKTDPTSAFGGII
AFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQNVRVLTCGQWASRVPGLDFKRVNGGLLVQDRDLGMVSEAE
LRVVSKRQPTEQELRDALFCWKVAKFVKSNAIVYAKENMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSAMASDAFFP
FRDGIDAAAAVGVSCVIQPGGSIRDDEVIAAADEHGIAMIFTDMRHFRH

Specific function: De novo purine biosynthesis; ninth step. De novo purine biosynthesis; tenth step. [C]

COG id: COG0138

COG function: function code F; AICAR transformylase/IMP cyclohydrolase PurH (only IMP cyclohydrolase domain in Aful)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purH family [H]

Homologues:

Organism=Homo sapiens, GI20127454, Length=486, Percent_Identity=39.3004115226337, Blast_Score=280, Evalue=3e-75,
Organism=Escherichia coli, GI1790439, Length=529, Percent_Identity=95.0850661625709, Blast_Score=1034, Evalue=0.0,
Organism=Caenorhabditis elegans, GI71985564, Length=614, Percent_Identity=34.2019543973941, Blast_Score=291, Evalue=8e-79,
Organism=Caenorhabditis elegans, GI71985574, Length=352, Percent_Identity=27.8409090909091, Blast_Score=91, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI71985556, Length=115, Percent_Identity=39.1304347826087, Blast_Score=79, Evalue=6e-15,
Organism=Saccharomyces cerevisiae, GI6323056, Length=490, Percent_Identity=35.9183673469388, Blast_Score=280, Evalue=5e-76,
Organism=Saccharomyces cerevisiae, GI6323768, Length=485, Percent_Identity=36.4948453608247, Blast_Score=275, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24649832, Length=611, Percent_Identity=35.5155482815057, Blast_Score=308, Evalue=6e-84,

Paralogues:

None

Copy number: 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 640 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002695
- InterPro:   IPR013982
- InterPro:   IPR016193
- InterPro:   IPR011607 [H]

Pfam domain/function: PF01808 AICARFT_IMPCHas; PF02142 MGS [H]

EC number: =2.1.2.3; =3.5.4.10 [H]

Molecular weight: Translated: 57369; Mature: 57369

Theoretical pI: Translated: 5.82; Mature: 5.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQQRRPVRRALLSVSDKAGIIEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGF
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEHHHCCCCCEECCCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDAIMEQHHIAPIDMVVVNLYPFAETVARVGCSLE
HHHHCCCEEEECCHHHCCCCCCCCCCCHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEMDANEGSLTLDTRFDLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEECCCEEHHHH
EHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEEHHHCCCCCCCCCCEEEEE
EENVKEASVATAQQVQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVAVSTSI
ECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEHHHHH
LDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQ
HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECC
NVRVLTCGQWASRVPGLDFKRVNGGLLVQDRDLGMVSEAELRVVSKRQPTEQELRDALFC
CCEEEECCCHHHHCCCCCEEECCCCEEEEECCCCCCCHHHHHEECCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYAKENMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSAMASDAFFP
HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCCEECCCEEECCCCCC
FRDGIDAAAAVGVSCVIQPGGSIRDDEVIAAADEHGIAMIFTDMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC
>Mature Secondary Structure
MQQRRPVRRALLSVSDKAGIIEFAQALSARGVELLSTGGTARLLAEKGLPVTEVSDYTGF
CCCCCHHHHHHHHCCCCCCHHHHHHHHHHCCEEEEECCCCEEEHHHCCCCCEECCCCCCC
PEMMDGRVKTLHPKVHGGILGRRGQDDAIMEQHHIAPIDMVVVNLYPFAETVARVGCSLE
HHHHCCCEEEECCHHHCCCCCCCCCCCHHHHHCCCCCCEEEEEEHHHHHHHHHHHCCCHH
DAVENIDIGGPTMVRSAAKNHKDVAIVVKSSDYDAIIKEMDANEGSLTLDTRFDLAIKAF
HHHHHCCCCCHHHHHHHHCCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEECCCEEHHHH
EHTAAYDSMIANYFGSMVPAYHGESKEAAGRFPRTLNLNFIKKQDMRYGENSHQQAAFYI
HHHHHHHHHHHHHHHHHCCCCCCCCHHHHCCCCCEEEEEEEEHHHCCCCCCCCCCEEEEE
EENVKEASVATAQQVQGKALSYNNIADTDAALECVKEFNEPACVIVKHANPCGVAVSTSI
ECCCHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCCCCCEEHHHHH
LDAYDRAYKTDPTSAFGGIIAFNRELDAETAQAIISRQFVEVIIAPSASEEALKITAAKQ
HHHHHHHHCCCCCHHHCCEEEECCCCCHHHHHHHHHHHHHHEEECCCCCCCEEEEEEECC
NVRVLTCGQWASRVPGLDFKRVNGGLLVQDRDLGMVSEAELRVVSKRQPTEQELRDALFC
CCEEEECCCHHHHCCCCCEEECCCCEEEEECCCCCCCHHHHHEECCCCCCHHHHHHHHHH
WKVAKFVKSNAIVYAKENMTIGIGAGQMSRVYSAKIAGIKAADEGLEVKGSAMASDAFFP
HHHHHHHHCCEEEEEECCEEEEECCCHHHHHHHHHHCCCEECCCCCEECCCEEECCCCCC
FRDGIDAAAAVGVSCVIQPGGSIRDDEVIAAADEHGIAMIFTDMRHFRH
CCCCCCHHHHCCEEEEECCCCCCCCCCEEEECCCCCEEEEEECHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA