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The map label for this gene is aes [H]

Identifier: 194738198

GI number: 194738198

Start: 556112

End: 557083

Strand: Reverse

Name: aes [H]

Synonym: SeSA_A0552

Alternate gene names: 194738198

Gene position: 557083-556112 (Counterclockwise)

Preceding gene: 194735414

Following gene: 194736637

Centisome position: 11.83

GC content: 56.38

Gene sequence:

>972_bases
ATGAAGCCGGAAAATAAAATTCCCGTACTCACCCGACTGTCAGACGAAATGACAGCCGTCGTAAACTTCCAGCAACCAGG
CCTTCCCCCCTGGCCTGCCGATGGCGATATTGAGACTCAGCGTCAGTATTATCTTCTTGAGCGCCGTTTCTGGAATGCCG
ACGCGCCGTCCATGACCACCCGCACCTGCGCCGTCCCTACGCCTTATGGCGATGTAACAACCCGGCTCTACTCGCCGCAG
CCGACCAGTCAGGCCATCTTATACTATCTGCACGGCGGCGGATTTATCCTCGGTAATCTGGATACGCATGACCGGATAAT
GCGTCTGCTGGCGCGTTATACCGGCTGCACCGTGATCGGTATTGATTATTCGCTGTCGCCGCAAGCGCGTTATCCGCAGG
CCATCGAAGAAACCGTCGCGGTATGCAGCTACTTCTCTCAACATGCCGATGAATACTCGCTCAACGTGGAGAAAATCGGT
TTCGCCGGGGATTCCGCAGGCGCCATGCTGGCGCTTGCCAGCGCGCTCTGGCTACGTGATAAGCACATTCGCTGCGGTAA
CGTTATCGCGATTCTGTTGTGGTATGGGCTGTATGGGTTGCAGGATTCGGTAAGCCGCCGTCTGTTCGGCGGCGCGTGGG
ACGGCCTGACGCGTGAAGATCTGGACATGTACGAAAAGGCCTATCTGCGTAACGAAGACGATCGGGAATCGCCCTGGTAT
TGTCTGTTTAATAACGATCTCACGCGCGATGTGCCGCCCTGCTTTATTGCCAGCGCGGAGTTTGATCCGCTCATTGATGA
CAGCCGCTTGCTGCACCAGACATTACAGGCGCACCAGCAGCCCTGCGAATACAAAATGTATCCCGGCACGCTGCACGCCT
TTCTGCACTATTCGCGAATGATGACAATCGCCGACGATGCCTTGCAGGACGGCGCGCGCTTCTTTATGGCGAGGATGAAG
ACGCCGCGTTAA

Upstream 100 bases:

>100_bases
GCTGCGCGCGACGGACGATGGCGTTTCAATATCGGACGACGAGATCGCAGTTTCTCCGGCCGCGTTTTTTACAATGGTTC
ACTTTCACAAGGAGCGTTTT

Downstream 100 bases:

>100_bases
CGGTACGGCGCCGTGAGTTTTAACATCATATCAATATGTTCCGGCGTCGCGTTCAGCGCCGGAATGTACGCATATTTTTT
ACCGCCCGCTTCAAGGAAAA

Product: acetyl esterase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MKPENKIPVLTRLSDEMTAVVNFQQPGLPPWPADGDIETQRQYYLLERRFWNADAPSMTTRTCAVPTPYGDVTTRLYSPQ
PTSQAILYYLHGGGFILGNLDTHDRIMRLLARYTGCTVIGIDYSLSPQARYPQAIEETVAVCSYFSQHADEYSLNVEKIG
FAGDSAGAMLALASALWLRDKHIRCGNVIAILLWYGLYGLQDSVSRRLFGGAWDGLTREDLDMYEKAYLRNEDDRESPWY
CLFNNDLTRDVPPCFIASAEFDPLIDDSRLLHQTLQAHQQPCEYKMYPGTLHAFLHYSRMMTIADDALQDGARFFMARMK
TPR

Sequences:

>Translated_323_residues
MKPENKIPVLTRLSDEMTAVVNFQQPGLPPWPADGDIETQRQYYLLERRFWNADAPSMTTRTCAVPTPYGDVTTRLYSPQ
PTSQAILYYLHGGGFILGNLDTHDRIMRLLARYTGCTVIGIDYSLSPQARYPQAIEETVAVCSYFSQHADEYSLNVEKIG
FAGDSAGAMLALASALWLRDKHIRCGNVIAILLWYGLYGLQDSVSRRLFGGAWDGLTREDLDMYEKAYLRNEDDRESPWY
CLFNNDLTRDVPPCFIASAEFDPLIDDSRLLHQTLQAHQQPCEYKMYPGTLHAFLHYSRMMTIADDALQDGARFFMARMK
TPR
>Mature_323_residues
MKPENKIPVLTRLSDEMTAVVNFQQPGLPPWPADGDIETQRQYYLLERRFWNADAPSMTTRTCAVPTPYGDVTTRLYSPQ
PTSQAILYYLHGGGFILGNLDTHDRIMRLLARYTGCTVIGIDYSLSPQARYPQAIEETVAVCSYFSQHADEYSLNVEKIG
FAGDSAGAMLALASALWLRDKHIRCGNVIAILLWYGLYGLQDSVSRRLFGGAWDGLTREDLDMYEKAYLRNEDDRESPWY
CLFNNDLTRDVPPCFIASAEFDPLIDDSRLLHQTLQAHQQPCEYKMYPGTLHAFLHYSRMMTIADDALQDGARFFMARMK
TPR

Specific function: Displays esterase activity towards short chain fatty esters (acyl chain length of up to 8 carbons). Able to hydrolyze triacetylglycerol (triacetin) and tributyrylglycerol (tributyrin), but not trioleylglycerol (triolein) or cholesterol oleate. Negatively

COG id: COG0657

COG function: function code I; Esterase/lipase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDXG' lipolytic enzyme family [H]

Homologues:

Organism=Homo sapiens, GI206597554, Length=142, Percent_Identity=33.0985915492958, Blast_Score=70, Evalue=3e-12,
Organism=Homo sapiens, GI68299767, Length=132, Percent_Identity=30.3030303030303, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI21328446, Length=125, Percent_Identity=32, Blast_Score=69, Evalue=8e-12,
Organism=Escherichia coli, GI1786682, Length=319, Percent_Identity=74.6081504702194, Blast_Score=511, Evalue=1e-146,
Organism=Caenorhabditis elegans, GI17540028, Length=182, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI72001146, Length=158, Percent_Identity=28.4810126582279, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI24656084, Length=108, Percent_Identity=32.4074074074074, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI24656076, Length=108, Percent_Identity=32.4074074074074, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI20130169, Length=108, Percent_Identity=32.4074074074074, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013094
- InterPro:   IPR002168 [H]

Pfam domain/function: PF07859 Abhydrolase_3 [H]

EC number: 3.1.1.-

Molecular weight: Translated: 36811; Mature: 36811

Theoretical pI: Translated: 5.37; Mature: 5.37

Prosite motif: PS01174 LIPASE_GDXG_SER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPENKIPVLTRLSDEMTAVVNFQQPGLPPWPADGDIETQRQYYLLERRFWNADAPSMTT
CCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
RTCAVPTPYGDVTTRLYSPQPTSQAILYYLHGGGFILGNLDTHDRIMRLLARYTGCTVIG
CEEECCCCCCCHHEEEECCCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHCCCEEEE
IDYSLSPQARYPQAIEETVAVCSYFSQHADEYSLNVEKIGFAGDSAGAMLALASALWLRD
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCEEEECCCCCCHHHHHHHHHHHHHC
KHIRCGNVIAILLWYGLYGLQDSVSRRLFGGAWDGLTREDLDMYEKAYLRNEDDRESPWY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEE
CLFNNDLTRDVPPCFIASAEFDPLIDDSRLLHQTLQAHQQPCEYKMYPGTLHAFLHYSRM
EEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHH
MTIADDALQDGARFFMARMKTPR
HHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MKPENKIPVLTRLSDEMTAVVNFQQPGLPPWPADGDIETQRQYYLLERRFWNADAPSMTT
CCCCCCCCEEEECCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCC
RTCAVPTPYGDVTTRLYSPQPTSQAILYYLHGGGFILGNLDTHDRIMRLLARYTGCTVIG
CEEECCCCCCCHHEEEECCCCCCCEEEEEEECCEEEEECCCHHHHHHHHHHHHCCCEEEE
IDYSLSPQARYPQAIEETVAVCSYFSQHADEYSLNVEKIGFAGDSAGAMLALASALWLRD
ECCCCCCCCCCHHHHHHHHHHHHHHHHCCHHHCCCCEEEECCCCCCHHHHHHHHHHHHHC
KHIRCGNVIAILLWYGLYGLQDSVSRRLFGGAWDGLTREDLDMYEKAYLRNEDDRESPWY
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEE
CLFNNDLTRDVPPCFIASAEFDPLIDDSRLLHQTLQAHQQPCEYKMYPGTLHAFLHYSRM
EEECCCCCCCCCCEEEECCCCCCCCCHHHHHHHHHHHHCCCCCEEECCHHHHHHHHHHHH
MTIADDALQDGARFFMARMKTPR
HHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds; Carboxylic ester hydrolases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA