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The map label for this gene is rfaF [H]

Identifier: 194737811

GI number: 194737811

Start: 3781606

End: 3782652

Strand: Direct

Name: rfaF [H]

Synonym: SeSA_A3910

Alternate gene names: 194737811

Gene position: 3781606-3782652 (Clockwise)

Preceding gene: 194734790

Following gene: 194737435

Centisome position: 80.3

GC content: 58.17

Gene sequence:

>1047_bases
ATGAAAATTTTGGTCATTGGCCCGTCCTGGGTGGGCGACATGATGATGTCGCAAAGTCTCTATCGCACGCTTAAAGCGCG
CTATCCCCAGGCGATAATCGACGTGATGGCGCCAGCCTGGTGCCGTCCGTTATTATCGCGTATGCCGGAAGTTAACGAGG
CGATACCCATGCCGCTGGGCCACGGCGCGCTGGAAATCGGCGAGCGCCGTAGATTGGGCCATAGCCTGCGTGAGAAGCGC
TACGATCGCGCCTGGGTGCTGCCAAATTCGTTTAAATCGGCGCTGATTCCTTTCTTTGCCAATATCCCGCACCGTACCGG
CTGGCGCGGCGAAATGCGCTATGGCCTGCTGAACGATGCGCGCGTCCTTGATAAAGACGCCTGGCCGCTGATGGTGGAGC
GCTACGTGGCGCTGGCCTATGACAAGGGCGTGATGCGCACGGCGAAAGATCTGCCGCAGCCGCTACTCTGGCCACAGCTC
CAGGTTAGCGAGGGTGAAAAGTCGCTGATGTGCAGCGACTTTTCACTATCTTCTGAACGTCCTCTGATCGGCTTTTGCCC
CGGCGCGGAATTTGGCCCGGCAAAACGTTGGCCGCACTATCACTACGCCGAACTGGCAAAACAGCTCATTAACGAAGGGT
ATCAGGTCGTACTGTTTGGCTCGGCAAAAGACCATGAAGCCGGAAATGAGATCCTGGCGGCGCTGAATAGCGAGCAGCAG
GCATGGTGTCGCAACCTGGCGGGGGAAACCCAGCTGGAACAGGCCGTCATTCTGATAGCCGCCTGTAAAGCCATCGTCAC
TAACGATTCCGGGCTGATGCACGTCGCGGCGGCGCTCGACCGCCCGCTGGTCGCCTTGTATGGCCCAAGTAGCCCGGATT
TCACGCCGCCGCTGTCTCATAAGGCCCGGGTGATTCGTCTCATTACGGGTTATCACAAAGTGCGTAAAGGTGATACGGCG
CAAGGCTATCACCAGAGCCTGATCGATATCACGCCGCAGCGGGTTCTGGAAGAGCTTCATTCGCTGTTGTCGGAAGAGGG
CGTTTAA

Upstream 100 bases:

>100_bases
ATTTAACCAATCTGCGCAACGCGGGCTACGACAAACCCTTTAAGACCGTCGCCGAAGGCGTCACGGAGTATATGGCCTGG
CTGAACCGCGACGCGTAAGT

Downstream 100 bases:

>100_bases
TGCGGGTTTTGATCGTTAAAACATCATCAATGGGCGACGTATTACATACCCTGCCTGCGCTTACCGACGCGCAACAGGCG
ATTCCGGGGATTCAATTTGA

Product: ADP-heptose:LPS heptosyltransferase II

Products: NA

Alternate protein names: ADP-heptose--LPS heptosyltransferase II [H]

Number of amino acids: Translated: 348; Mature: 348

Protein sequence:

>348_residues
MKILVIGPSWVGDMMMSQSLYRTLKARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLGHGALEIGERRRLGHSLREKR
YDRAWVLPNSFKSALIPFFANIPHRTGWRGEMRYGLLNDARVLDKDAWPLMVERYVALAYDKGVMRTAKDLPQPLLWPQL
QVSEGEKSLMCSDFSLSSERPLIGFCPGAEFGPAKRWPHYHYAELAKQLINEGYQVVLFGSAKDHEAGNEILAALNSEQQ
AWCRNLAGETQLEQAVILIAACKAIVTNDSGLMHVAAALDRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDTA
QGYHQSLIDITPQRVLEELHSLLSEEGV

Sequences:

>Translated_348_residues
MKILVIGPSWVGDMMMSQSLYRTLKARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLGHGALEIGERRRLGHSLREKR
YDRAWVLPNSFKSALIPFFANIPHRTGWRGEMRYGLLNDARVLDKDAWPLMVERYVALAYDKGVMRTAKDLPQPLLWPQL
QVSEGEKSLMCSDFSLSSERPLIGFCPGAEFGPAKRWPHYHYAELAKQLINEGYQVVLFGSAKDHEAGNEILAALNSEQQ
AWCRNLAGETQLEQAVILIAACKAIVTNDSGLMHVAAALDRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDTA
QGYHQSLIDITPQRVLEELHSLLSEEGV
>Mature_348_residues
MKILVIGPSWVGDMMMSQSLYRTLKARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLGHGALEIGERRRLGHSLREKR
YDRAWVLPNSFKSALIPFFANIPHRTGWRGEMRYGLLNDARVLDKDAWPLMVERYVALAYDKGVMRTAKDLPQPLLWPQL
QVSEGEKSLMCSDFSLSSERPLIGFCPGAEFGPAKRWPHYHYAELAKQLINEGYQVVLFGSAKDHEAGNEILAALNSEQQ
AWCRNLAGETQLEQAVILIAACKAIVTNDSGLMHVAAALDRPLVALYGPSSPDFTPPLSHKARVIRLITGYHKVRKGDTA
QGYHQSLIDITPQRVLEELHSLLSEEGV

Specific function: Lipopolysaccharide core biosynthesis. [C]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790050, Length=346, Percent_Identity=92.7745664739884, Blast_Score=677, Evalue=0.0,
Organism=Escherichia coli, GI1790051, Length=149, Percent_Identity=31.5436241610738, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011910 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 39016; Mature: 39016

Theoretical pI: Translated: 8.22; Mature: 8.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILVIGPSWVGDMMMSQSLYRTLKARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLG
CEEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCHHHHHHHHHHCCCCHHCCCCCCC
HGALEIGERRRLGHSLREKRYDRAWVLPNSFKSALIPFFANIPHRTGWRGEMRYGLLNDA
CCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCC
RVLDKDAWPLMVERYVALAYDKGVMRTAKDLPQPLLWPQLQVSEGEKSLMCSDFSLSSER
HHHCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEECCCCCCEEECCCCCCCCC
PLIGFCPGAEFGPAKRWPHYHYAELAKQLINEGYQVVLFGSAKDHEAGNEILAALNSEQQ
CEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCHHH
AWCRNLAGETQLEQAVILIAACKAIVTNDSGLMHVAAALDRPLVALYGPSSPDFTPPLSH
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCH
KARVIRLITGYHKVRKGDTAQGYHQSLIDITPQRVLEELHSLLSEEGV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKILVIGPSWVGDMMMSQSLYRTLKARYPQAIIDVMAPAWCRPLLSRMPEVNEAIPMPLG
CEEEEECCHHHHHHHHHHHHHHHHHHHCHHHHHHHHCHHHHHHHHHHCCCCHHCCCCCCC
HGALEIGERRRLGHSLREKRYDRAWVLPNSFKSALIPFFANIPHRTGWRGEMRYGLLNDA
CCHHHHHHHHHHHHHHHHHHCCCEEECCHHHHHHHHHHHHCCCCCCCCCCCCEECCCCCC
RVLDKDAWPLMVERYVALAYDKGVMRTAKDLPQPLLWPQLQVSEGEKSLMCSDFSLSSER
HHHCCCCCHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCCEEECCCCCCEEECCCCCCCCC
PLIGFCPGAEFGPAKRWPHYHYAELAKQLINEGYQVVLFGSAKDHEAGNEILAALNSEQQ
CEEEECCCCCCCCCCCCCCCHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHHHCCHHH
AWCRNLAGETQLEQAVILIAACKAIVTNDSGLMHVAAALDRPLVALYGPSSPDFTPPLSH
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEECCCCCCCCCCCCH
KARVIRLITGYHKVRKGDTAQGYHQSLIDITPQRVLEELHSLLSEEGV
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8041620; 9278503; 2198271; 8478319 [H]