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The map label for this gene is pncA [H]

Identifier: 194737759

GI number: 194737759

Start: 1337787

End: 1338443

Strand: Reverse

Name: pncA [H]

Synonym: SeSA_A1387

Alternate gene names: 194737759

Gene position: 1338443-1337787 (Counterclockwise)

Preceding gene: 194737093

Following gene: 194734935

Centisome position: 28.42

GC content: 57.84

Gene sequence:

>657_bases
ATGACAAACCGGGCCTTATTACTGGTCGACTTGCAAAATGACTTTTGCGCCGGCGGCGCGCTGGCTGTAGCGGAAGGCGA
CAGCACCATCGACATCGCCAACGCTCTTATTGACTGGTGCCAGCCCCGGCAGATTCCGGTGCTGGCCAGCCAGGACTGGC
ATCCCGCGCAGCATGGCAGCTTCGCCAGCCAGCATCAGGCGGAACCCTATAGTCAGGGAAAGCTGGACGGGCTGCCGCAA
ACGCTCTGGCCGGATCACTGCGTTCAACATACCGACGGCGCGGCGTTACATCCCCTGCTTAACCAACATGCGATTGACGC
CTGCATCTTTAAGGGTGAAAACCCGCTGATTGATAGCTACAGCGCGTTTTTTGACAACGAACATCGGCAGAAAACTACCC
TTGATACCTGGCTTCGCGAGCATGACGTTACCGAGCTTATCGTGATGGGGTTGGCGACCGATTACTGCGTAAAATTTACG
GTGCTGGATGCGCTAGAGTTGGGCTATGCCGTTAACGTTATCACCGATGGCTGTCGCGGCGTGAATATTCACCCGCAGGA
TAGCGCACACGCGTTTATGGAGATGGCGGCGGCAGGCGCGACGCTGTATACGCTGGCAGACTGGGAAGAGACGCAGGGCC
AGGCTGTCGCCAGGTAG

Upstream 100 bases:

>100_bases
AGGGTCTGGACACTCAGGCGATTCGTAGCGCGATGGCGCAAAACTTGCGTGGTGAATTGACGCCGGATGATTAAAAAATC
CCGACGACAAGGAGAAGATA

Downstream 100 bases:

>100_bases
CGGAGCAGGTATCGTATCCGGTCAATACATCACGCAGCGGTGAGACCATTGCATAGATCTTTGGGCCGGATACGTCACGT
CAGCGTCGCTACCAGGCAAA

Product: nicotinamidase/pyrazinamidase

Products: NA

Alternate protein names: PZAase; Nicotine deamidase; NAMase [H]

Number of amino acids: Translated: 218; Mature: 217

Protein sequence:

>218_residues
MTNRALLLVDLQNDFCAGGALAVAEGDSTIDIANALIDWCQPRQIPVLASQDWHPAQHGSFASQHQAEPYSQGKLDGLPQ
TLWPDHCVQHTDGAALHPLLNQHAIDACIFKGENPLIDSYSAFFDNEHRQKTTLDTWLREHDVTELIVMGLATDYCVKFT
VLDALELGYAVNVITDGCRGVNIHPQDSAHAFMEMAAAGATLYTLADWEETQGQAVAR

Sequences:

>Translated_218_residues
MTNRALLLVDLQNDFCAGGALAVAEGDSTIDIANALIDWCQPRQIPVLASQDWHPAQHGSFASQHQAEPYSQGKLDGLPQ
TLWPDHCVQHTDGAALHPLLNQHAIDACIFKGENPLIDSYSAFFDNEHRQKTTLDTWLREHDVTELIVMGLATDYCVKFT
VLDALELGYAVNVITDGCRGVNIHPQDSAHAFMEMAAAGATLYTLADWEETQGQAVAR
>Mature_217_residues
TNRALLLVDLQNDFCAGGALAVAEGDSTIDIANALIDWCQPRQIPVLASQDWHPAQHGSFASQHQAEPYSQGKLDGLPQT
LWPDHCVQHTDGAALHPLLNQHAIDACIFKGENPLIDSYSAFFDNEHRQKTTLDTWLREHDVTELIVMGLATDYCVKFTV
LDALELGYAVNVITDGCRGVNIHPQDSAHAFMEMAAAGATLYTLADWEETQGQAVAR

Specific function: NAD biosynthesis; nicotinamide to NaMN; first step. [C]

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pncA family [H]

Homologues:

Organism=Escherichia coli, GI87081970, Length=213, Percent_Identity=80.7511737089202, Blast_Score=363, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI193206735, Length=216, Percent_Identity=34.2592592592593, Blast_Score=117, Evalue=4e-27,
Organism=Caenorhabditis elegans, GI17543130, Length=216, Percent_Identity=34.2592592592593, Blast_Score=116, Evalue=7e-27,
Organism=Caenorhabditis elegans, GI71999339, Length=213, Percent_Identity=32.3943661971831, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6321401, Length=201, Percent_Identity=33.3333333333333, Blast_Score=104, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648247, Length=204, Percent_Identity=37.2549019607843, Blast_Score=117, Evalue=5e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: =3.5.1.19 [H]

Molecular weight: Translated: 23909; Mature: 23778

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNRALLLVDLQNDFCAGGALAVAEGDSTIDIANALIDWCQPRQIPVLASQDWHPAQHGS
CCCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCC
FASQHQAEPYSQGKLDGLPQTLWPDHCVQHTDGAALHPLLNQHAIDACIFKGENPLIDSY
CCHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHEEECCCCCCHHHH
SAFFDNEHRQKTTLDTWLREHDVTELIVMGLATDYCVKFTVLDALELGYAVNVITDGCRG
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
VNIHPQDSAHAFMEMAAAGATLYTLADWEETQGQAVAR
CCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC
>Mature Secondary Structure 
TNRALLLVDLQNDFCAGGALAVAEGDSTIDIANALIDWCQPRQIPVLASQDWHPAQHGS
CCCEEEEEECCCCCCCCCEEEEECCCCHHHHHHHHHHHCCCCCCCEEECCCCCCCCCCC
FASQHQAEPYSQGKLDGLPQTLWPDHCVQHTDGAALHPLLNQHAIDACIFKGENPLIDSY
CCHHHCCCCCCCCCCCCCCCCCCCHHHHHCCCCCHHHHHHHHHHHHHEEECCCCCCHHHH
SAFFDNEHRQKTTLDTWLREHDVTELIVMGLATDYCVKFTVLDALELGYAVNVITDGCRG
HHHHCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEEEECCCCC
VNIHPQDSAHAFMEMAAAGATLYTLADWEETQGQAVAR
CCCCCCCHHHHHHHHHHCCCEEEEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2670682; 8726014; 9097039; 9278503 [H]