Click here to switch to the map view.

The map label for this gene is alaS [H]

Identifier: 194737652

GI number: 194737652

Start: 2878565

End: 2881195

Strand: Reverse

Name: alaS [H]

Synonym: SeSA_A2978

Alternate gene names: 194737652

Gene position: 2881195-2878565 (Counterclockwise)

Preceding gene: 194738077

Following gene: 194734626

Centisome position: 61.18

GC content: 54.85

Gene sequence:

>2631_bases
ATGAGCAAGAGCACCGCTGAGATCCGTCAGGCGTTTCTCGACTTTTTCCATAGTAAAGGGCATCAGGTAGTTGCCAGCAG
TTCCCTGGTGCCTAACAATGACCCCACTCTGTTGTTTACCAATGCCGGGATGAACCAGTTCAAGGATGTTTTCCTTGGGC
TCGATAAACGTAATTATTCCCGTGCAACCACTTCCCAGCGTTGCGTACGCGCGGGGGGTAAACACAACGATCTGGAAAAC
GTCGGATACACTGCACGTCACCATACTTTCTTCGAAATGCTGGGCAACTTCAGCTTCGGCGATTACTTCAAACACGACGC
CATTCAGTTCGCATGGGAATTACTGACCGGTGAAAACTGGTTTGCTCTGCCAAAAGAGCGTTTATGGGTCACCGTCTATG
AAACCGACGACGAAGCCTATGAGATCTGGGAAAAAGAAGTCGGCATCCCGCGCGAGCGTATCATCCGTATCGGCGACAAT
AAAGGCGCGCCATACGCATCGGATAACTTCTGGCAGATGGGCGACACCGGTCCCTGCGGTCCGTGCACCGAAATCTTCTA
CGATCATGGCGACCACATCTGGGGCGGCCCTCCGGGAAGCCCGGAAGAAGACGGCGATCGCTATATTGAGATCTGGAACA
TCGTCTTTATGCAGTTCAACCGTCAGGCTGACGGGACGATGGAACCATTGCCGAAACCGTCTGTAGATACCGGTATGGGG
CTGGAGCGTATTGCCGCGGTACTGCAACACGTTAACTCTAACTATGACATCGACCTGTTCCGCACGTTGATTGAAGCGGT
AGCGAAAGTCACCGGTGCCACCGATCTGGGTAATAAATCGCTGCGCGTTATCGCCGACCACATCCGTTCCTGCGCATTCC
TGGTTGCCGATGGCGTGCTGCCGTCGAATGAAAACCGTGGCTATGTGCTGCGTCGCATTATTCGTCGCGCGGTGCGTCAC
GGCAACATGCTGGGCGCGAAAGAGACCTTCTTCTACAAGCTGGTTGGGCCGCTGATTGAGGTAATGGGCTCCGCAGGCGA
AGAGTTGAAACGTCAGCAGGCGCAGGTCGAGCAGGTTCTGAAAACGGAAGAAGAGCAGTTCGCCCGTACGCTGGAGCGCG
GTCTGGCGCTGCTGGATGAAGAACTGGCGAAATTGCAGGGTGATACCCTGGACGGCGAAACCGCTTTCCGTCTGTATGAT
ACTTATGGTTTCCCGGTCGATTTAACGGCGGACGTCTGCCGTGAGCGCAATATCAAAGTCGACGAAGCCGGTTTTGAAGC
AGCCATGGAAGAGCAACGCCGTCGCGCGCGCGAAGCTAGCGGTTTTGGCGCGGACTACAACGCGATGATCCGCGTTGATA
GCGCTTCTGAATTTAAAGGTTATGACCATCTGGAACTGAACGGCAAAGTGACTGCGCTGTTTGTCGATGGTAAAGCGGTT
GAAGCGATTAACGCGGGACAGGAAGCGGTTGTCGTGCTGGATCAGACCCCATTCTATGCGGAATCAGGCGGTCAGGTTGG
CGATAAAGGCGAGCTGAAAGGCGCTGGATTTACTTTCGCTGTGGACGACACGCAAAAATATGGTCAGGCGATTGGTCATA
TCGGCAAATTGTCTGCGGGCGCTCTGAAAGTGGGCGATGCGGTACAGGCTGACGTAGATGAAGCGCGCCGCGCACGTATC
CGTCTGAACCACTCCGCCACGCACCTGATGCACGCGGCGTTGCGCCAGGTGCTTGGGACACACGTTGCACAGAAAGGTTC
GCTGGTTAGCGATAAAGTGCTGCGCTTCGACTTCTCGCACAATGAAGCGATGAAGCCGTCGGAGATTCGTCAGGTTGAAG
ATTTGGTGAATGCGCAAATTCGCCGCAACCTGCCGATTGAAACGAACATCATGGATCTCGACGCGGCGAAGGCGAAAGGC
GCCATGGCGCTGTTTGGCGAGAAATATGACGAACGTGTACGCGTTCTGAGCATGGGCGATTTCTCTACCGAACTGTGCGG
CGGTACTCACGCCAGTCGTACCGGTGATATCGGTCTATTCCGTATTATCTCTGAGTCCGGCACCGCAGCGGGCATTCGTC
GTATTGAGGCGGTAACCGGTGAAGGCGCTATGGCCACCGTTCATGCGCAAAGCGATCGCTTAAACGATATTGCGCATCTG
CTGAAGGGCGACAGCCAGAATCTGGGCGACAAAGTGCGTGCCGTGCTGGAACGTACACGTCAGCTGGAAAAAGAGTTGCA
GCAGTTGAAGGACCAGGCCGCAGCGCAGGAGAGTGCAAATCTTTCCAGTAAAGCGGTCGATCTCAACGGCGTGAAGCTCC
TGGTCAGCGAGCTTGCTAGTATTGAGCCGAAAATGCTGCGAACCATGGTTGATGATCTGAAAAATCAACTGGGGTCTACC
GTTATCGTACTGGCAACGGTTGTTGAAGGTAAGGTTTCTCTGATTGCGGGCGTGTCGAAGGATGTGACCGACCGGGTTAA
AGCAGGGGAATTGATTGGTATGGTCGCTCAGCAGGTAGGCGGTAAGGGTGGCGGTCGTCCGGACATGGCGCAAGCCGGTG
GTACGGATGCGGCAGCTCTGCCTGCAGCGTTAGCCAGTGTACAAGGCTGGGTCAGCGCAAAATTGCAATAA

Upstream 100 bases:

>100_bases
TCAAAGCGGCAAGTGAGCCTCGGGAACGCGTGACCGGGAAACTCGCAGCCAACGCAGAGGCGGCCTGAAGGATGAAGTGT
AGCTTGATTTCAGGATAATT

Downstream 100 bases:

>100_bases
TTATAAGCGTTCGTTGGCGCCGCAGCCCGTGCTGCGGCGTTTACGTCAACGCTATCGACAACGATAAAGTCAGGTTGAAG
TTTTGTATATCGGCTAAACT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 876; Mature: 875

Protein sequence:

>876_residues
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLEN
VGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTGENWFALPKERLWVTVYETDDEAYEIWEKEVGIPRERIIRIGDN
KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
LERIAAVLQHVNSNYDIDLFRTLIEAVAKVTGATDLGNKSLRVIADHIRSCAFLVADGVLPSNENRGYVLRRIIRRAVRH
GNMLGAKETFFYKLVGPLIEVMGSAGEELKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLQGDTLDGETAFRLYD
TYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAV
EAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGAGFTFAVDDTQKYGQAIGHIGKLSAGALKVGDAVQADVDEARRARI
RLNHSATHLMHAALRQVLGTHVAQKGSLVSDKVLRFDFSHNEAMKPSEIRQVEDLVNAQIRRNLPIETNIMDLDAAKAKG
AMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGIRRIEAVTGEGAMATVHAQSDRLNDIAHL
LKGDSQNLGDKVRAVLERTRQLEKELQQLKDQAAAQESANLSSKAVDLNGVKLLVSELASIEPKMLRTMVDDLKNQLGST
VIVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTDAAALPAALASVQGWVSAKLQ

Sequences:

>Translated_876_residues
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLEN
VGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTGENWFALPKERLWVTVYETDDEAYEIWEKEVGIPRERIIRIGDN
KGAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
LERIAAVLQHVNSNYDIDLFRTLIEAVAKVTGATDLGNKSLRVIADHIRSCAFLVADGVLPSNENRGYVLRRIIRRAVRH
GNMLGAKETFFYKLVGPLIEVMGSAGEELKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLQGDTLDGETAFRLYD
TYGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAV
EAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGAGFTFAVDDTQKYGQAIGHIGKLSAGALKVGDAVQADVDEARRARI
RLNHSATHLMHAALRQVLGTHVAQKGSLVSDKVLRFDFSHNEAMKPSEIRQVEDLVNAQIRRNLPIETNIMDLDAAKAKG
AMALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGIRRIEAVTGEGAMATVHAQSDRLNDIAHL
LKGDSQNLGDKVRAVLERTRQLEKELQQLKDQAAAQESANLSSKAVDLNGVKLLVSELASIEPKMLRTMVDDLKNQLGST
VIVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTDAAALPAALASVQGWVSAKLQ
>Mature_875_residues
SKSTAEIRQAFLDFFHSKGHQVVASSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRNYSRATTSQRCVRAGGKHNDLENV
GYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTGENWFALPKERLWVTVYETDDEAYEIWEKEVGIPRERIIRIGDNK
GAPYASDNFWQMGDTGPCGPCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMGL
ERIAAVLQHVNSNYDIDLFRTLIEAVAKVTGATDLGNKSLRVIADHIRSCAFLVADGVLPSNENRGYVLRRIIRRAVRHG
NMLGAKETFFYKLVGPLIEVMGSAGEELKRQQAQVEQVLKTEEEQFARTLERGLALLDEELAKLQGDTLDGETAFRLYDT
YGFPVDLTADVCRERNIKVDEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAVE
AINAGQEAVVVLDQTPFYAESGGQVGDKGELKGAGFTFAVDDTQKYGQAIGHIGKLSAGALKVGDAVQADVDEARRARIR
LNHSATHLMHAALRQVLGTHVAQKGSLVSDKVLRFDFSHNEAMKPSEIRQVEDLVNAQIRRNLPIETNIMDLDAAKAKGA
MALFGEKYDERVRVLSMGDFSTELCGGTHASRTGDIGLFRIISESGTAAGIRRIEAVTGEGAMATVHAQSDRLNDIAHLL
KGDSQNLGDKVRAVLERTRQLEKELQQLKDQAAAQESANLSSKAVDLNGVKLLVSELASIEPKMLRTMVDDLKNQLGSTV
IVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVGGKGGGRPDMAQAGGTDAAALPAALASVQGWVSAKLQ

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=979, Percent_Identity=38.8151174668029, Blast_Score=563, Evalue=1e-160,
Organism=Homo sapiens, GI38569417, Length=963, Percent_Identity=35.202492211838, Blast_Score=479, Evalue=1e-135,
Organism=Escherichia coli, GI1789048, Length=876, Percent_Identity=95.4337899543379, Blast_Score=1722, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=990, Percent_Identity=37.0707070707071, Blast_Score=547, Evalue=1e-156,
Organism=Caenorhabditis elegans, GI17536681, Length=743, Percent_Identity=33.9165545087483, Blast_Score=387, Evalue=1e-107,
Organism=Saccharomyces cerevisiae, GI6324911, Length=768, Percent_Identity=43.2291666666667, Blast_Score=582, Evalue=1e-167,
Organism=Drosophila melanogaster, GI24582809, Length=956, Percent_Identity=38.0753138075314, Blast_Score=565, Evalue=1e-161,
Organism=Drosophila melanogaster, GI45552267, Length=956, Percent_Identity=38.0753138075314, Blast_Score=565, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24658214, Length=806, Percent_Identity=32.1339950372208, Blast_Score=357, Evalue=2e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 95929; Mature: 95798

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKSTAEIRQAFLDFFHSKGHQVVASSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRNYS
CCCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCC
RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTGENW
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
FALPKERLWVTVYETDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCG
EECCCCCEEEEEEECCCHHHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCEECCCCCCCC
PCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
LERIAAVLQHVNSNYDIDLFRTLIEAVAKVTGATDLGNKSLRVIADHIRSCAFLVADGVL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIEVMGSAGEELKRQQAQVEQVL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KTEEEQFARTLERGLALLDEELAKLQGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKV
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCC
DEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAV
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCCEEEECCEEEEEEECCCHH
EAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGAGFTFAVDDTQKYGQAIGHIGKLSAG
HHHCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCC
ALKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVAQKGSLVSDKVLRFDFSH
CEECCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCC
NEAMKPSEIRQVEDLVNAQIRRNLPIETNIMDLDAAKAKGAMALFGEKYDERVRVLSMGD
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHCCHHHHHHCCHHCCEEEEEECC
FSTELCGGTHASRTGDIGLFRIISESGTAAGIRRIEAVTGEGAMATVHAQSDRLNDIAHL
CCHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
LKGDSQNLGDKVRAVLERTRQLEKELQQLKDQAAAQESANLSSKAVDLNGVKLLVSELAS
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHC
IEPKMLRTMVDDLKNQLGSTVIVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVG
CCHHHHHHHHHHHHHHHCCEEEEEHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GKGGGRPDMAQAGGTDAAALPAALASVQGWVSAKLQ
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKSTAEIRQAFLDFFHSKGHQVVASSSLVPNNDPTLLFTNAGMNQFKDVFLGLDKRNYS
CCCHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCCC
RATTSQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKHDAIQFAWELLTGENW
HHHHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCC
FALPKERLWVTVYETDDEAYEIWEKEVGIPRERIIRIGDNKGAPYASDNFWQMGDTGPCG
EECCCCCEEEEEEECCCHHHHHHHHHHCCCHHHEEEECCCCCCCCCCCCCEECCCCCCCC
PCTEIFYDHGDHIWGGPPGSPEEDGDRYIEIWNIVFMQFNRQADGTMEPLPKPSVDTGMG
HHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCC
LERIAAVLQHVNSNYDIDLFRTLIEAVAKVTGATDLGNKSLRVIADHIRSCAFLVADGVL
HHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCC
PSNENRGYVLRRIIRRAVRHGNMLGAKETFFYKLVGPLIEVMGSAGEELKRQQAQVEQVL
CCCCCCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
KTEEEQFARTLERGLALLDEELAKLQGDTLDGETAFRLYDTYGFPVDLTADVCRERNIKV
HCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCHHHHHCCCCCC
DEAGFEAAMEEQRRRAREASGFGADYNAMIRVDSASEFKGYDHLELNGKVTALFVDGKAV
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCHHCCCCCEEEECCEEEEEEECCCHH
EAINAGQEAVVVLDQTPFYAESGGQVGDKGELKGAGFTFAVDDTQKYGQAIGHIGKLSAG
HHHCCCCCEEEEEECCCCEECCCCCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHCCCCC
ALKVGDAVQADVDEARRARIRLNHSATHLMHAALRQVLGTHVAQKGSLVSDKVLRFDFSH
CEECCCHHHHHHHHHHHHHEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHEEEECCC
NEAMKPSEIRQVEDLVNAQIRRNLPIETNIMDLDAAKAKGAMALFGEKYDERVRVLSMGD
CCCCCHHHHHHHHHHHHHHHHCCCCCCCCEEECCHHHHCCHHHHHHCCHHCCEEEEEECC
FSTELCGGTHASRTGDIGLFRIISESGTAAGIRRIEAVTGEGAMATVHAQSDRLNDIAHL
CCHHHCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCHHHHHHHHHH
LKGDSQNLGDKVRAVLERTRQLEKELQQLKDQAAAQESANLSSKAVDLNGVKLLVSELAS
HCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCHHHHHHHHHHC
IEPKMLRTMVDDLKNQLGSTVIVLATVVEGKVSLIAGVSKDVTDRVKAGELIGMVAQQVG
CCHHHHHHHHHHHHHHHCCEEEEEHHHHCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHC
GKGGGRPDMAQAGGTDAAALPAALASVQGWVSAKLQ
CCCCCCCCHHHCCCCCHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA