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The map label for this gene is smbA [H]

Identifier: 194737631

GI number: 194737631

Start: 443206

End: 444426

Strand: Direct

Name: smbA [H]

Synonym: SeSA_A0430

Alternate gene names: 194737631

Gene position: 443206-444426 (Clockwise)

Preceding gene: 194735140

Following gene: 194737237

Centisome position: 9.41

GC content: 51.68

Gene sequence:

>1221_bases
ATGTTTAAATCTTTTTTCCCAAAGCCAGGGCCGTTTTTCATGTCGGCGTTTGTTTGGGCGCTGATCGCCGTCATTTTCTG
GCAGGCGGGAGGAGGAGATTGGGTGGCGCGACTTGTAGGGGCATCCGATGAAGTGCCTATCAGCGCAGCGCGTTTCTGGT
CGCTGGACTATCTGATTTTTTATGCTTATTACCTGATTTGCGTGGGATTATTTGCAACGTTCTGGTTCATCTATAGCCCG
CACCGCTGGCAGTACTGGTCGATACTTGGCACCTCGCTCATTATTTTTGTCACCTGGTTTTTGGTGGAGGTTGGCGTCGC
GGTTAACGCCTGGTACGCGCCGTTCTATGATTTGATTCAAACGGCGCTCAGCTCGCCGCACAAGGTCACTCTTGGTCAAT
TTTATCATGAAGTGGGGGTGTTTCTCGGTATTGCGCTTATCGCCGTGGTGATCGGCGTGCTGAACAACTTCTTTGTCAGC
CACTATGTATTTCGCTGGCGTACCGCAATGAATGAGCACTATATGGCGCACTGGCAGTACCTGCGCCATATCGAAGGGGC
CGCCCAACGTGTGCAGGAAGACACCATGCGTTTTGCCTCTACACTGGAGAATATGGGCGTGAGTTTTATCAATGCCATCA
TGACGCTCATCGCCTTTCTGCCGGTGTTGGTCACCCTGTCAGCGCATGTGCCGAATTTACCCATCGTAGGGCATATCCCT
TACGGCCTGGTCATTGCCGCTATAGTCTGGTCATTAATGGGGACGGGCCTGCTGGCGGTCGTCGGGATCAAATTGCCGGG
GCTGGAATTTAAAAATCAGCGAGTGGAAGCCGCTTATCGTAAGGAGCTGGTCTACGGTGAAGATGACGCCAGCCGCGCCA
CGCCGCCAACCGTCCGTGAACTGTTTAGCGCGGTGCGCCATAACTACTTCCGTCTCTACTTTCACTATATGTATTTCAAC
ATCGCGCGCATTCTCTATTTGCAGGTCGATAACGTTTTCGGTTTGTTCCTGCTGTTTCCTTCCATTGTTGCCGGTACGAT
TACGCTGGGGCTGATGACGCAAATCACCAACGTGTTTGGCCAGGTGCGCGGTTCGTTCCAGTATTTGATTAACTCCTGGA
CGACTCTGGTGGAGCTGATGTCTATTTATAAACGTTTACGTAGCTTTGAACGCCAGTTGGATGGTCAGCCAGTTCAGGAA
GTGACCCACTCTTTTAGCTAA

Upstream 100 bases:

>100_bases
CACAATGCCTGGTTTAGCCCTTTCATTTGCGTGGGCATGGCGTGATATTGCCGCCGTTACAGCAGCCGTAATAAAGAGAA
GAAGTTAGCAGGAGAGCATG

Downstream 100 bases:

>100_bases
CAGAAGGAAACGTTATGTCGGCGGCGTCCCGTCTTTATCCTCTGCCTTTTCTGGCGGTTGCCATATTGGCTGGCTGTAGT
AGTCAATCCGGGCAGCCGGT

Product: transport protein

Products: ADP; phosphate; microcin B17 [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 406; Mature: 406

Protein sequence:

>406_residues
MFKSFFPKPGPFFMSAFVWALIAVIFWQAGGGDWVARLVGASDEVPISAARFWSLDYLIFYAYYLICVGLFATFWFIYSP
HRWQYWSILGTSLIIFVTWFLVEVGVAVNAWYAPFYDLIQTALSSPHKVTLGQFYHEVGVFLGIALIAVVIGVLNNFFVS
HYVFRWRTAMNEHYMAHWQYLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIAFLPVLVTLSAHVPNLPIVGHIP
YGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVEAAYRKELVYGEDDASRATPPTVRELFSAVRHNYFRLYFHYMYFN
IARILYLQVDNVFGLFLLFPSIVAGTITLGLMTQITNVFGQVRGSFQYLINSWTTLVELMSIYKRLRSFERQLDGQPVQE
VTHSFS

Sequences:

>Translated_406_residues
MFKSFFPKPGPFFMSAFVWALIAVIFWQAGGGDWVARLVGASDEVPISAARFWSLDYLIFYAYYLICVGLFATFWFIYSP
HRWQYWSILGTSLIIFVTWFLVEVGVAVNAWYAPFYDLIQTALSSPHKVTLGQFYHEVGVFLGIALIAVVIGVLNNFFVS
HYVFRWRTAMNEHYMAHWQYLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIAFLPVLVTLSAHVPNLPIVGHIP
YGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVEAAYRKELVYGEDDASRATPPTVRELFSAVRHNYFRLYFHYMYFN
IARILYLQVDNVFGLFLLFPSIVAGTITLGLMTQITNVFGQVRGSFQYLINSWTTLVELMSIYKRLRSFERQLDGQPVQE
VTHSFS
>Mature_406_residues
MFKSFFPKPGPFFMSAFVWALIAVIFWQAGGGDWVARLVGASDEVPISAARFWSLDYLIFYAYYLICVGLFATFWFIYSP
HRWQYWSILGTSLIIFVTWFLVEVGVAVNAWYAPFYDLIQTALSSPHKVTLGQFYHEVGVFLGIALIAVVIGVLNNFFVS
HYVFRWRTAMNEHYMAHWQYLRHIEGAAQRVQEDTMRFASTLENMGVSFINAIMTLIAFLPVLVTLSAHVPNLPIVGHIP
YGLVIAAIVWSLMGTGLLAVVGIKLPGLEFKNQRVEAAYRKELVYGEDDASRATPPTVRELFSAVRHNYFRLYFHYMYFN
IARILYLQVDNVFGLFLLFPSIVAGTITLGLMTQITNVFGQVRGSFQYLINSWTTLVELMSIYKRLRSFERQLDGQPVQE
VTHSFS

Specific function: Required for the uptake of the peptide antibiotic microcin B17 (MccB17) [H]

COG id: COG1133

COG function: function code I; ABC-type long-chain fatty acid transport system, fused permease and ATPase components

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: To R.meliloti BacA [H]

Homologues:

Organism=Escherichia coli, GI1786575, Length=406, Percent_Identity=92.3645320197044, Blast_Score=772, Evalue=0.0,
Organism=Escherichia coli, GI1787772, Length=326, Percent_Identity=25.1533742331288, Blast_Score=82, Evalue=4e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011527
- InterPro:   IPR009248 [H]

Pfam domain/function: PF05992 SbmA_BacA [H]

EC number: NA

Molecular weight: Translated: 46450; Mature: 46450

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
>Mature Secondary Structure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HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; microcin B17 [Periplasm]; H2O [C]

Specific reaction: ATP + microcin B17 [Periplasm] + H2O = ADP + phosphate + microcin B17 [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]