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The map label for this gene is purR [H]

Identifier: 194737554

GI number: 194737554

Start: 3703217

End: 3704224

Strand: Direct

Name: purR [H]

Synonym: SeSA_A3832

Alternate gene names: 194737554

Gene position: 3703217-3704224 (Clockwise)

Preceding gene: 194737871

Following gene: 194736349

Centisome position: 78.64

GC content: 60.22

Gene sequence:

>1008_bases
ATGGCTGTTCAAAATAAAAAACGCGCAAAGTTGATTGATGTTGCCCGCCATGCAGGCGTGTCGCCAGGGACGGTATCCAA
TGCCTTGCACAACACCCGCTTTGTCGAGCCGCAGACGCGACGGCGTATTGAAGAGGCCATTGTTGCGCTCAACTACACGC
CGAATATTCGCGCCCGCCAGTTGCGAACCGGCAAAACCAATACCATTGCCTTGCTCTCTTCGGTGCCGCTGGCGATTGCC
TCCGGTGCGTCACGACTGGGATTTATGATGGAGGTGGCGTTAACGTCCGCGATGATGGCGCTGGAAAAACAGCATGCGCT
GATTCTGGTGCCGCCGGGGGCAAATCCACTGGATGCCGTCAGCTTTGACGCGGCGATCCTGATTGAGCCGGCGGAGAACG
ACCCGCAGCTCCAGGCGCTGGCGCAAGCGGGCATTCCCTGCGTCACCATTGGCCGCACGCCGGGCGCCGACACGCCTGTG
CCGTGGGTTGAGCTGCACTCGGCGGCAACAGCACAGCTTCTGCTAACGCATCTGGAGGCCTCCGGCGCCAGCAAATGTGC
GTTATTTGTCGGTAACACTCGGCGAACATCAGTTCAGGAGAGCGAAGCGGCTTACCAGCGCTGGTGCGCGGGGCGCCAGG
ACCCCGTCGTCTACTCTCTCAATGAAAGCGAGGGTGAAAATGCCGGCTACCAGGCCGCGCAGCAGCTATTACAGGCGCAT
CCCGACGTTGACGGCGTGCTGGTGCTGATCGATACCTTTGCCAGCGGCGCGGTACGCGCTTTTCAGGAACAGGCAGTAGC
TATCCCGGAGCGGATGCGCGTGGTCACCCGCTATGATGGTATCCGCGCGCGCGAATCGCTGCCGCCGCTGACGGCAGTGA
ATATGCATCTTGATGAGGTGGCGCGACAGGCAATCACACTATTATTTGATTTGCTGGCGGGCAAGAAGGTCAGTCACAGC
GACGGGATCATGCCTGAACTGGTGGTGCGAGCGTCAACCTGCTGGTGA

Upstream 100 bases:

>100_bases
TTAATGAAGAGTTTTTTACGATCCTCACCGAAAAAAAGCAAACGTTACCGTCTGGTAAATATGCTACCCTGTGCGCAAAC
TTTCGAAGGTCAGGAAGCAG

Downstream 100 bases:

>100_bases
TAGAGGATGACTCGCCACTGCGTGGCTCGCCCTGCGGGCCGTTGCTGACGCAACGTTCTCTCGCTGCGCTCGAGTCGAAC
CTCTTGCCCCCGGAGGTTCT

Product: regulatory protein LacI:Periplasmic binding protein/LacI transcriptional regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 335; Mature: 334

Protein sequence:

>335_residues
MAVQNKKRAKLIDVARHAGVSPGTVSNALHNTRFVEPQTRRRIEEAIVALNYTPNIRARQLRTGKTNTIALLSSVPLAIA
SGASRLGFMMEVALTSAMMALEKQHALILVPPGANPLDAVSFDAAILIEPAENDPQLQALAQAGIPCVTIGRTPGADTPV
PWVELHSAATAQLLLTHLEASGASKCALFVGNTRRTSVQESEAAYQRWCAGRQDPVVYSLNESEGENAGYQAAQQLLQAH
PDVDGVLVLIDTFASGAVRAFQEQAVAIPERMRVVTRYDGIRARESLPPLTAVNMHLDEVARQAITLLFDLLAGKKVSHS
DGIMPELVVRASTCW

Sequences:

>Translated_335_residues
MAVQNKKRAKLIDVARHAGVSPGTVSNALHNTRFVEPQTRRRIEEAIVALNYTPNIRARQLRTGKTNTIALLSSVPLAIA
SGASRLGFMMEVALTSAMMALEKQHALILVPPGANPLDAVSFDAAILIEPAENDPQLQALAQAGIPCVTIGRTPGADTPV
PWVELHSAATAQLLLTHLEASGASKCALFVGNTRRTSVQESEAAYQRWCAGRQDPVVYSLNESEGENAGYQAAQQLLQAH
PDVDGVLVLIDTFASGAVRAFQEQAVAIPERMRVVTRYDGIRARESLPPLTAVNMHLDEVARQAITLLFDLLAGKKVSHS
DGIMPELVVRASTCW
>Mature_334_residues
AVQNKKRAKLIDVARHAGVSPGTVSNALHNTRFVEPQTRRRIEEAIVALNYTPNIRARQLRTGKTNTIALLSSVPLAIAS
GASRLGFMMEVALTSAMMALEKQHALILVPPGANPLDAVSFDAAILIEPAENDPQLQALAQAGIPCVTIGRTPGADTPVP
WVELHSAATAQLLLTHLEASGASKCALFVGNTRRTSVQESEAAYQRWCAGRQDPVVYSLNESEGENAGYQAAQQLLQAHP
DVDGVLVLIDTFASGAVRAFQEQAVAIPERMRVVTRYDGIRARESLPPLTAVNMHLDEVARQAITLLFDLLAGKKVSHSD
GIMPELVVRASTCW

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790194, Length=317, Percent_Identity=27.1293375394322, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1786540, Length=341, Percent_Identity=25.8064516129032, Blast_Score=76, Evalue=4e-15,
Organism=Escherichia coli, GI1790369, Length=350, Percent_Identity=24, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1787580, Length=328, Percent_Identity=24.6951219512195, Blast_Score=68, Evalue=1e-12,
Organism=Escherichia coli, GI1790715, Length=331, Percent_Identity=23.2628398791541, Blast_Score=65, Evalue=7e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36047; Mature: 35916

Theoretical pI: Translated: 7.24; Mature: 7.24

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVQNKKRAKLIDVARHAGVSPGTVSNALHNTRFVEPQTRRRIEEAIVALNYTPNIRARQ
CCCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCCCHHH
LRTGKTNTIALLSSVPLAIASGASRLGFMMEVALTSAMMALEKQHALILVPPGANPLDAV
CCCCCCCEEEEHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
SFDAAILIEPAENDPQLQALAQAGIPCVTIGRTPGADTPVPWVELHSAATAQLLLTHLEA
CCCEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
SGASKCALFVGNTRRTSVQESEAAYQRWCAGRQDPVVYSLNESEGENAGYQAAQQLLQAH
CCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHC
PDVDGVLVLIDTFASGAVRAFQEQAVAIPERMRVVTRYDGIRARESLPPLTAVNMHLDEV
CCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEHHHHHHHH
ARQAITLLFDLLAGKKVSHSDGIMPELVVRASTCW
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCC
>Mature Secondary Structure 
AVQNKKRAKLIDVARHAGVSPGTVSNALHNTRFVEPQTRRRIEEAIVALNYTPNIRARQ
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHEEECCCCCCCHHH
LRTGKTNTIALLSSVPLAIASGASRLGFMMEVALTSAMMALEKQHALILVPPGANPLDAV
CCCCCCCEEEEHHHCCHHHHCCCHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCC
SFDAAILIEPAENDPQLQALAQAGIPCVTIGRTPGADTPVPWVELHSAATAQLLLTHLEA
CCCEEEEEECCCCCHHHHHHHHCCCCEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHC
SGASKCALFVGNTRRTSVQESEAAYQRWCAGRQDPVVYSLNESEGENAGYQAAQQLLQAH
CCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHHHHHHC
PDVDGVLVLIDTFASGAVRAFQEQAVAIPERMRVVTRYDGIRARESLPPLTAVNMHLDEV
CCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCEEHHHHHHHH
ARQAITLLFDLLAGKKVSHSDGIMPELVVRASTCW
HHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA