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The map label for this gene is gltD [H]

Identifier: 194737546

GI number: 194737546

Start: 3398126

End: 3399544

Strand: Direct

Name: gltD [H]

Synonym: SeSA_A3524

Alternate gene names: 194737546

Gene position: 3398126-3399544 (Clockwise)

Preceding gene: 194737893

Following gene: 194736738

Centisome position: 72.16

GC content: 57.01

Gene sequence:

>1419_bases
ATGAGCCAGAATGTTTATCAATTTATCGACCTGCAGCGCGTTGATCCGCCGAAGAAACCGCTGAAGATCCGTAAGATCGA
GTTTGTTGAAATCTACGAACCGTTTTCTGAAGGTCAGGCGAAAGCCCAGGCGGATCGCTGTTTGTCCTGCGGGAACCCCT
ACTGCGAGTGGAAATGTCCGGTACATAACTACATCCCGAACTGGCTGAAACTGGCTAACGAAGGGCGTATTTTTGAGGCG
GCTGAGCTATCGCACCAGACCAACACCCTGCCGGAAGTGTGTGGCCGCGTCTGCCCGCAGGACCGCCTGTGCGAAGGCTC
CTGTACGCTGCACGACGAGTTCGGCGCAGTAACCATCGGCAACATCGAACGTTATATCAACGATAAAGCGTTCGAAATGG
GCTGGCGCCCGGATATGACCGGCGTGCGCCAGACGGACAAACGGGTAGCGATTATCGGCGCAGGTCCGGCAGGGCTGGCG
TGCGCCGACGTGCTGACCCGCAACGGCGTGAAGGCGGTGGTCTTTGATCGCCATCCGGAAATCGGCGGCCTGCTGACCTT
CGGTATTCCAGCTTTCAAACTGGAAAAAGAGGTGATGACCCGCCGTCGCGAGATCTTCACCGGCATGGGTATTGAGTTCA
AACTTAATACCGAAGTGGGCCGCGACGTCGAACTTGACGAGCTGTTGAAAGAGTACGACGCGGTATTCCTTGGCGTCGGC
ACCTATCAGTCGATGCGCGGCGGGCTGGAAAATGAAAATGCGGACGGCGTATTCGACGCTCTGCCGTTCCTGATCGCCAA
TACGAAGCAGATCATGGGGTTTGGCGAAACCAGCGACGAACCATATGTCAGCATGGAAGGCAAACGCGTGGTGGTGCTGG
GCGGCGGCGATACCGCAATGGACTGCGTACGTACCTCCATTCGTCAGGGCGCAACGCACGTAACCTGTGCTTATCGTCGT
GATGAAGAGAACATGCCGGGCTCCAGACGCGAAGTGAAGAACGCGCGCGAGGAAGGTGTCGAATTTCAGTTTAACGTTCA
ACCGCTGGGCATTGAAGTCAATGCCAACGGCAAAGTGAGCGGCGTAAAAATGGTGCGCACTGAAATGGGCGAACCGGATG
CCAAAGGCCGTCGGCGGGCGGAGATCGTCGCCGGTTCCGAGCATGTGGTTCCGGCTGATGCGGTGGTGATGGCGTTTGGT
TTTCGTCCACACAGCATGGAATGGCTGGCGAAACACAGCGTGGAACTGGACTCGCAGGGACGAATTATCGCGCCGGAACG
CAGCGATAATGCTTTCCAGACCAGCAACCCGAAAATCTTCGCCGGTGGCGACATCGTGCGCGGTTCCGATCTGGTGGTAA
CGGCGATTGCCGAAGGCCGTAAGGCGGCTGACGGGATTATGAACTACCTCGAAGTGTAA

Upstream 100 bases:

>100_bases
CGCGTTAGTGAAGCCCAAGTCCAGCGATGTCAAAGCCCTGTTGGGCCACCGTAGTCGTAGTGCGGCAGAATTGCGCGTGC
AGGCGCAGTAAGGAATTGAA

Downstream 100 bases:

>100_bases
CCCTTCCGGCTCCCCGGTTGATGCCTTGTGCTGATGGCTGGGGAGCCGATATTCCTCTCCTCAATATCTCTCCTGCCGAT
TCTGGAAACCCGCTCGTAAA

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEA
AELSHQTNTLPEVCGRVCPQDRLCEGSCTLHDEFGAVTIGNIERYINDKAFEMGWRPDMTGVRQTDKRVAIIGAGPAGLA
CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVELDELLKEYDAVFLGVG
TYQSMRGGLENENADGVFDALPFLIANTKQIMGFGETSDEPYVSMEGKRVVVLGGGDTAMDCVRTSIRQGATHVTCAYRR
DEENMPGSRREVKNAREEGVEFQFNVQPLGIEVNANGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHVVPADAVVMAFG
FRPHSMEWLAKHSVELDSQGRIIAPERSDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV

Sequences:

>Translated_472_residues
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEA
AELSHQTNTLPEVCGRVCPQDRLCEGSCTLHDEFGAVTIGNIERYINDKAFEMGWRPDMTGVRQTDKRVAIIGAGPAGLA
CADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVELDELLKEYDAVFLGVG
TYQSMRGGLENENADGVFDALPFLIANTKQIMGFGETSDEPYVSMEGKRVVVLGGGDTAMDCVRTSIRQGATHVTCAYRR
DEENMPGSRREVKNAREEGVEFQFNVQPLGIEVNANGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHVVPADAVVMAFG
FRPHSMEWLAKHSVELDSQGRIIAPERSDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV
>Mature_471_residues
SQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCPVHNYIPNWLKLANEGRIFEAA
ELSHQTNTLPEVCGRVCPQDRLCEGSCTLHDEFGAVTIGNIERYINDKAFEMGWRPDMTGVRQTDKRVAIIGAGPAGLAC
ADVLTRNGVKAVVFDRHPEIGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVELDELLKEYDAVFLGVGT
YQSMRGGLENENADGVFDALPFLIANTKQIMGFGETSDEPYVSMEGKRVVVLGGGDTAMDCVRTSIRQGATHVTCAYRRD
EENMPGSRREVKNAREEGVEFQFNVQPLGIEVNANGKVSGVKMVRTEMGEPDAKGRRRAEIVAGSEHVVPADAVVMAFGF
RPHSMEWLAKHSVELDSQGRIIAPERSDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=492, Percent_Identity=28.4552845528455, Blast_Score=165, Evalue=8e-41,
Organism=Escherichia coli, GI1789606, Length=472, Percent_Identity=94.9152542372881, Blast_Score=937, Evalue=0.0,
Organism=Escherichia coli, GI1788811, Length=461, Percent_Identity=66.3774403470716, Blast_Score=655, Evalue=0.0,
Organism=Escherichia coli, GI87082180, Length=450, Percent_Identity=58.6666666666667, Blast_Score=556, Evalue=1e-160,
Organism=Escherichia coli, GI1788468, Length=442, Percent_Identity=31.447963800905, Blast_Score=182, Evalue=3e-47,
Organism=Escherichia coli, GI1789243, Length=425, Percent_Identity=32, Blast_Score=154, Evalue=9e-39,
Organism=Caenorhabditis elegans, GI17570289, Length=494, Percent_Identity=33.6032388663968, Blast_Score=224, Evalue=9e-59,
Organism=Caenorhabditis elegans, GI71984108, Length=466, Percent_Identity=30.68669527897, Blast_Score=164, Evalue=1e-40,
Organism=Caenorhabditis elegans, GI17543860, Length=161, Percent_Identity=29.8136645962733, Blast_Score=75, Evalue=9e-14,
Organism=Saccharomyces cerevisiae, GI6320030, Length=469, Percent_Identity=32.409381663113, Blast_Score=226, Evalue=9e-60,
Organism=Drosophila melanogaster, GI28574881, Length=485, Percent_Identity=39.7938144329897, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24665539, Length=485, Percent_Identity=39.7938144329897, Blast_Score=297, Evalue=1e-80,
Organism=Drosophila melanogaster, GI24665547, Length=485, Percent_Identity=39.7938144329897, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24665543, Length=485, Percent_Identity=39.7938144329897, Blast_Score=296, Evalue=2e-80,
Organism=Drosophila melanogaster, GI24640763, Length=460, Percent_Identity=27.3913043478261, Blast_Score=152, Evalue=4e-37,
Organism=Drosophila melanogaster, GI18858217, Length=460, Percent_Identity=27.3913043478261, Blast_Score=152, Evalue=4e-37,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52233; Mature: 52102

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCP
CCCCHHHHHHHCCCCCCCCCCEEEEEEEEEEECCHHCCHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDRLCEGSCTLHDEFGAVTIG
HHHHCCHHHHHCCCCCEEEHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEECCCCEEEEC
NIERYINDKAFEMGWRPDMTGVRQTDKRVAIIGAGPAGLACADVLTRNGVKAVVFDRHPE
CHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVELDELLKEYDAVFLGVG
CCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCEEEEECC
TYQSMRGGLENENADGVFDALPFLIANTKQIMGFGETSDEPYVSMEGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHEECCCCCCCCEEEECCCEEEEECCCCHHH
DCVRTSIRQGATHVTCAYRRDEENMPGSRREVKNAREEGVEFQFNVQPLGIEVNANGKVS
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEEEECCCCCC
GVKMVRTEMGEPDAKGRRRAEIVAGSEHVVPADAVVMAFGFRPHSMEWLAKHSVELDSQG
CEEEEEHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCC
RIIAPERSDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV
CEECCCCCCCCEECCCCEEEECCCEEECCCEEEEEECCCCHHHHHHHHHHCC
>Mature Secondary Structure 
SQNVYQFIDLQRVDPPKKPLKIRKIEFVEIYEPFSEGQAKAQADRCLSCGNPYCEWKCP
CCCHHHHHHHCCCCCCCCCCEEEEEEEEEEECCHHCCHHHHHHHHHHHCCCCCEEEECC
VHNYIPNWLKLANEGRIFEAAELSHQTNTLPEVCGRVCPQDRLCEGSCTLHDEFGAVTIG
HHHHCCHHHHHCCCCCEEEHHHHCCCCCCHHHHHHHHCCCCCCCCCCCEEECCCCEEEEC
NIERYINDKAFEMGWRPDMTGVRQTDKRVAIIGAGPAGLACADVLTRNGVKAVVFDRHPE
CHHHHHCCHHHCCCCCCCCCCCCCCCCEEEEEECCCCHHHHHHHHHCCCCEEEEEECCCC
IGGLLTFGIPAFKLEKEVMTRRREIFTGMGIEFKLNTEVGRDVELDELLKEYDAVFLGVG
CCCEEEECCCHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHCEEEEECC
TYQSMRGGLENENADGVFDALPFLIANTKQIMGFGETSDEPYVSMEGKRVVVLGGGDTAM
CHHHHHCCCCCCCCCCHHHHHHHHHCCCHHHEECCCCCCCCEEEECCCEEEEECCCCHHH
DCVRTSIRQGATHVTCAYRRDEENMPGSRREVKNAREEGVEFQFNVQPLGIEVNANGKVS
HHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHCCCEEEEEEEEEEEEEECCCCCC
GVKMVRTEMGEPDAKGRRRAEIVAGSEHVVPADAVVMAFGFRPHSMEWLAKHSVELDSQG
CEEEEEHHCCCCCCCCCCEEEEEECCCCCCCCCCEEEEECCCCCCHHHHHHCCCCCCCCC
RIIAPERSDNAFQTSNPKIFAGGDIVRGSDLVVTAIAEGRKAADGIMNYLEV
CEECCCCCCCCEECCCCEEEECCCEEECCCEEEEEECCCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]