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The map label for this gene is polB [H]

Identifier: 194737454

GI number: 194737454

Start: 113225

End: 115576

Strand: Reverse

Name: polB [H]

Synonym: SeSA_A0109

Alternate gene names: 194737454

Gene position: 115576-113225 (Counterclockwise)

Preceding gene: 194734850

Following gene: 194738085

Centisome position: 2.45

GC content: 56.72

Gene sequence:

>2352_bases
GTGGCGCAGGCAGGTTTCATTTTAACCCGGCACTGGCGGGATACGCCGCAAGGAACGGAGGTCTCTTTCTGGCTGGCGAC
AGACAACGGCCCTGTGCAGGCGACGCTGGCGCCGCAGGAGTCCGTGGCGTTTATTCCGACCAGCCAGACCTCCCGCGCCG
CCTCGCTGCTACAGGCGGAAAAAGACTATCGCCTGACGCCGCTCCAGCTGCGAGACTTTCATCGTCAGCCGGTATCCGGT
CTGTATTGCCGCACTCACCGCCAGCTTATGCGGATGGAGAAACTGCTGCGCGAAAACGGCGTCACCGTTTATGAAGCGGA
TGTACGCCCGCCGGAACGTTACCTGATGGAACGTTTTATTACGTCGCCCGTTTGGGTAGACGGCGAGATGCGCAACGGCG
TTATCCGCAATGCGCGTCTTAAACCCCACCCCGACTATCGACCGCCGCTAAAATGGGTCTCGCTGGATATCGAAACCACG
CGACACGGCGAACTGTATTGCATTGGTCTGGAAGGCTGCGGCCAGCGTACCGTTTATATGCTGGGCCCGGCGAATGGCGA
TGACCGCCAACTGGACTTCGAGCTTGTGTATGTCGCCAGCCGCCCGCAGCTGCTGGAAAAACTCAACGCCTGGTTTGCCG
AACATGATCCCGACGTCATCATCGGCTGGAACGTGGTGCAGTTCGACCTGCGTATGCTGCAAAAACATGCCGAACGCTAC
CGTATCCCTTTACGGCTGGGGCGAGACAATAGCGAACTGGAGTGGCGCGAACATGGCTTCAAAAACGGCGTTTTTTTCGC
CCAGGCCAGAGGGCGAGTCATTATCGATGGTATCGACGCGCTTAAATCCGCGTTCTGGAATTTTTCGTCTTTTTCGCTGG
AAGCCGTCTCGCAGGAACTGCTCGGCGAAGGAAAATCCATTGATAATCCCTGGGATCGTATGGATGAGATCGATCGCCGC
TTTGCCCAGGATAAACCCGCCCTCGCCACCTACAACCTGAAAGACTGCGAGCTGGTCACGCGCATTTTCCATAAAACCGA
GATCATGCCGTTTTTGCTGGAACGCGCGACGATCAACGGCCTGCCGGTCGATCGCCACGGCGGTTCGGTCGCCGCATTTG
GGCATCTTTACTTTCCGCGAATGCACCGCGCCGGCTATGTCGCGCCCAATCTCGGCGAAGTGCCGCCGCTGGCAAGTCCC
GGCGGCTACGTAATGGATTCACAACCCGGATTATACGATTCAGTACTGGTGCTGGATTATAAAAGCCTGTACCCGTCGAT
TATCCGCACGTTTTTGATTGATCCCGTCGGGCTGGTCGAAGGCATGGCGCAGCCGGATCCGGAACACAGTACCGAAGGTT
TCCTTGACGCCTGGTTTTCACGAGAAAAGCACTGCCTGCCGGAAATTGTTAGCCAAATCTGGCACGGGCGCGATGAAGCC
AAACGTCAGGGTAATAAACCGCTCTCGCAGGCGCTTAAAATCATTATGAACGCCTTTTATGGCGTACTCGGCACCACGGC
CTGTCGCTTTTTCGATCCCCGTCTGGCGTCGTCAATCACCATGCGCGGCCATGCGATTATGCGCCAGACCAAAGCGCTGA
TTGAGGCGCAGGGTTATAACGTAATTTATGGCGATACCGATTCCACTTTCGTCTGGCTCCGGCGCGCGCATTCAGAAGCC
GACGCGGCGGAAATCGGGCACAGGCTGGTGCGTCACGTGAACGAATGGTGGGCGCAGACGTTACAACAGCAAAATTTGAC
CAGCGCGCTGGAGCTGGAGTTTGAAACCCACTTTTGCCGTTTTCTGATGCCGACCATCCGCGGCGCGGATACTGGCAGTA
AAAAACGCTACGCGGGCCTGATTCAGGAAGGCGACAGTCAGCGCATGGTATTTAAAGGTCTGGAAACGGTGCGGACCGAC
TGGACGCCGCTGGCGCAGCGCTTTCAACAGGAGCTGTATCTGCGCGTTTTTCGCAATGAACCTTATCAAGACTATGTGCG
CGAGACTATTGATAAACTGATGGCGGGCGAACTGGACGCGCAGCTGGTTTATCGCAAGCGTCTGCGTCGCCCTTTACATG
AGTACCAACGTAACATCCCGCCGCACGTTCGCGCCGCCCGGTTGGCGGATGAACAAAACCTGAAGCGGGGTCGTCCGGCG
CAGTATCAGAATCGCGGCGCCATAAAATATGTCTGGACTGTTAACGGCCCCGAGCCCGTGGATTACCAGCAGTCGCCGCT
GGATTACGAACACTATCTTACCCGCCAGCTACAACCCGTGGCGGAAGGAATTCTTCCCTTCGTTGAAGATAATTTTGCTA
CACTACTGACCGGGCAACTGGGGCTATTTTGA

Upstream 100 bases:

>100_bases
AGCGGATATCGGCTTTTACGTTATATGCCTGTACAAAATAACAGTATTACCCCCTAAAACAGGTTATAATTAGCGTGAAT
TTTTTAGACAGGTAATCATC

Downstream 100 bases:

>100_bases
CGCGTGACGAAAGCGCCGCCTTCCAGTACCATAGCGCCCTTTCCATTCCTGGACCTAATTAACGCCACTACCACATTAGC
CTGGTAGCGGGGTCGTATAC

Product: DNA polymerase II

Products: NA

Alternate protein names: Pol II [H]

Number of amino acids: Translated: 783; Mature: 782

Protein sequence:

>783_residues
MAQAGFILTRHWRDTPQGTEVSFWLATDNGPVQATLAPQESVAFIPTSQTSRAASLLQAEKDYRLTPLQLRDFHRQPVSG
LYCRTHRQLMRMEKLLRENGVTVYEADVRPPERYLMERFITSPVWVDGEMRNGVIRNARLKPHPDYRPPLKWVSLDIETT
RHGELYCIGLEGCGQRTVYMLGPANGDDRQLDFELVYVASRPQLLEKLNAWFAEHDPDVIIGWNVVQFDLRMLQKHAERY
RIPLRLGRDNSELEWREHGFKNGVFFAQARGRVIIDGIDALKSAFWNFSSFSLEAVSQELLGEGKSIDNPWDRMDEIDRR
FAQDKPALATYNLKDCELVTRIFHKTEIMPFLLERATINGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLGEVPPLASP
GGYVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVSQIWHGRDEA
KRQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHAIMRQTKALIEAQGYNVIYGDTDSTFVWLRRAHSEA
DAAEIGHRLVRHVNEWWAQTLQQQNLTSALELEFETHFCRFLMPTIRGADTGSKKRYAGLIQEGDSQRMVFKGLETVRTD
WTPLAQRFQQELYLRVFRNEPYQDYVRETIDKLMAGELDAQLVYRKRLRRPLHEYQRNIPPHVRAARLADEQNLKRGRPA
QYQNRGAIKYVWTVNGPEPVDYQQSPLDYEHYLTRQLQPVAEGILPFVEDNFATLLTGQLGLF

Sequences:

>Translated_783_residues
MAQAGFILTRHWRDTPQGTEVSFWLATDNGPVQATLAPQESVAFIPTSQTSRAASLLQAEKDYRLTPLQLRDFHRQPVSG
LYCRTHRQLMRMEKLLRENGVTVYEADVRPPERYLMERFITSPVWVDGEMRNGVIRNARLKPHPDYRPPLKWVSLDIETT
RHGELYCIGLEGCGQRTVYMLGPANGDDRQLDFELVYVASRPQLLEKLNAWFAEHDPDVIIGWNVVQFDLRMLQKHAERY
RIPLRLGRDNSELEWREHGFKNGVFFAQARGRVIIDGIDALKSAFWNFSSFSLEAVSQELLGEGKSIDNPWDRMDEIDRR
FAQDKPALATYNLKDCELVTRIFHKTEIMPFLLERATINGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLGEVPPLASP
GGYVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVSQIWHGRDEA
KRQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHAIMRQTKALIEAQGYNVIYGDTDSTFVWLRRAHSEA
DAAEIGHRLVRHVNEWWAQTLQQQNLTSALELEFETHFCRFLMPTIRGADTGSKKRYAGLIQEGDSQRMVFKGLETVRTD
WTPLAQRFQQELYLRVFRNEPYQDYVRETIDKLMAGELDAQLVYRKRLRRPLHEYQRNIPPHVRAARLADEQNLKRGRPA
QYQNRGAIKYVWTVNGPEPVDYQQSPLDYEHYLTRQLQPVAEGILPFVEDNFATLLTGQLGLF
>Mature_782_residues
AQAGFILTRHWRDTPQGTEVSFWLATDNGPVQATLAPQESVAFIPTSQTSRAASLLQAEKDYRLTPLQLRDFHRQPVSGL
YCRTHRQLMRMEKLLRENGVTVYEADVRPPERYLMERFITSPVWVDGEMRNGVIRNARLKPHPDYRPPLKWVSLDIETTR
HGELYCIGLEGCGQRTVYMLGPANGDDRQLDFELVYVASRPQLLEKLNAWFAEHDPDVIIGWNVVQFDLRMLQKHAERYR
IPLRLGRDNSELEWREHGFKNGVFFAQARGRVIIDGIDALKSAFWNFSSFSLEAVSQELLGEGKSIDNPWDRMDEIDRRF
AQDKPALATYNLKDCELVTRIFHKTEIMPFLLERATINGLPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLGEVPPLASPG
GYVMDSQPGLYDSVLVLDYKSLYPSIIRTFLIDPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVSQIWHGRDEAK
RQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHAIMRQTKALIEAQGYNVIYGDTDSTFVWLRRAHSEAD
AAEIGHRLVRHVNEWWAQTLQQQNLTSALELEFETHFCRFLMPTIRGADTGSKKRYAGLIQEGDSQRMVFKGLETVRTDW
TPLAQRFQQELYLRVFRNEPYQDYVRETIDKLMAGELDAQLVYRKRLRRPLHEYQRNIPPHVRAARLADEQNLKRGRPAQ
YQNRGAIKYVWTVNGPEPVDYQQSPLDYEHYLTRQLQPVAEGILPFVEDNFATLLTGQLGLF

Specific function: Thought to be involved in DNA repair and/or mutagenesis [H]

COG id: COG0417

COG function: function code L; DNA polymerase elongation subunit (family B)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA polymerase type-B family [H]

Homologues:

Organism=Homo sapiens, GI156616275, Length=657, Percent_Identity=26.7884322678843, Blast_Score=135, Evalue=1e-31,
Organism=Homo sapiens, GI106507301, Length=642, Percent_Identity=24.4548286604361, Blast_Score=122, Evalue=1e-27,
Organism=Escherichia coli, GI1786246, Length=783, Percent_Identity=89.9106002554278, Blast_Score=1484, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17559488, Length=714, Percent_Identity=25.9103641456583, Blast_Score=146, Evalue=5e-35,
Organism=Caenorhabditis elegans, GI32565317, Length=642, Percent_Identity=24.2990654205607, Blast_Score=132, Evalue=8e-31,
Organism=Saccharomyces cerevisiae, GI6320101, Length=632, Percent_Identity=25, Blast_Score=129, Evalue=2e-30,
Organism=Saccharomyces cerevisiae, GI6324227, Length=549, Percent_Identity=23.1329690346084, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6325090, Length=655, Percent_Identity=22.1374045801527, Blast_Score=82, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24648780, Length=645, Percent_Identity=23.5658914728682, Blast_Score=123, Evalue=4e-28,
Organism=Drosophila melanogaster, GI24664937, Length=800, Percent_Identity=24, Blast_Score=120, Evalue=2e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006172
- InterPro:   IPR017964
- InterPro:   IPR006133
- InterPro:   IPR006134
- InterPro:   IPR023211
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00136 DNA_pol_B; PF03104 DNA_pol_B_exo [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 90064; Mature: 89932

Theoretical pI: Translated: 7.26; Mature: 7.26

Prosite motif: PS00116 DNA_POLYMERASE_B

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAGFILTRHWRDTPQGTEVSFWLATDNGPVQATLAPQESVAFIPTSQTSRAASLLQAE
CCCCCEEEEECCCCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEECCCHHHHHHHHHHC
KDYRLTPLQLRDFHRQPVSGLYCRTHRQLMRMEKLLRENGVTVYEADVRPPERYLMERFI
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
TSPVWVDGEMRNGVIRNARLKPHPDYRPPLKWVSLDIETTRHGELYCIGLEGCGQRTVYM
CCCEEECCHHHCCCEECCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEEE
LGPANGDDRQLDFELVYVASRPQLLEKLNAWFAEHDPDVIIGWNVVQFDLRMLQKHAERY
EECCCCCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHE
RIPLRLGRDNSELEWREHGFKNGVFFAQARGRVIIDGIDALKSAFWNFSSFSLEAVSQEL
ECCEEECCCCCCCHHHHCCCCCCEEEEECCCCEEEECHHHHHHHHCCCCCHHHHHHHHHH
LGEGKSIDNPWDRMDEIDRRFAQDKPALATYNLKDCELVTRIFHKTEIMPFLLERATING
HCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLGEVPPLASPGGYVMDSQPGLYDSVLVLDY
CCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHEEEH
KSLYPSIIRTFLIDPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVSQIWHGRDEA
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHH
KRQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHAIMRQTKALIEAQGYN
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VIYGDTDSTFVWLRRAHSEADAAEIGHRLVRHVNEWWAQTLQQQNLTSALELEFETHFCR
EEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHH
FLMPTIRGADTGSKKRYAGLIQEGDSQRMVFKGLETVRTDWTPLAQRFQQELYLRVFRNE
HHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
PYQDYVRETIDKLMAGELDAQLVYRKRLRRPLHEYQRNIPPHVRAARLADEQNLKRGRPA
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCCCCC
QYQNRGAIKYVWTVNGPEPVDYQQSPLDYEHYLTRQLQPVAEGILPFVEDNFATLLTGQL
CCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHH
GLF
CCC
>Mature Secondary Structure 
AQAGFILTRHWRDTPQGTEVSFWLATDNGPVQATLAPQESVAFIPTSQTSRAASLLQAE
CCCCEEEEECCCCCCCCCEEEEEEEECCCCEEEEECCCCCEEEEECCCHHHHHHHHHHC
KDYRLTPLQLRDFHRQPVSGLYCRTHRQLMRMEKLLRENGVTVYEADVRPPERYLMERFI
CCCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCHHHHHHHHHH
TSPVWVDGEMRNGVIRNARLKPHPDYRPPLKWVSLDIETTRHGELYCIGLEGCGQRTVYM
CCCEEECCHHHCCCEECCCCCCCCCCCCCCEEEEEEEEECCCCCEEEEEECCCCCEEEEE
LGPANGDDRQLDFELVYVASRPQLLEKLNAWFAEHDPDVIIGWNVVQFDLRMLQKHAERY
EECCCCCCCEEEEEEEEEECCHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHE
RIPLRLGRDNSELEWREHGFKNGVFFAQARGRVIIDGIDALKSAFWNFSSFSLEAVSQEL
ECCEEECCCCCCCHHHHCCCCCCEEEEECCCCEEEECHHHHHHHHCCCCCHHHHHHHHHH
LGEGKSIDNPWDRMDEIDRRFAQDKPALATYNLKDCELVTRIFHKTEIMPFLLERATING
HCCCCCCCCHHHHHHHHHHHHHCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHCCC
LPVDRHGGSVAAFGHLYFPRMHRAGYVAPNLGEVPPLASPGGYVMDSQPGLYDSVLVLDY
CCCCCCCCCEEEEHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEECCCCCCCHHHHEEEH
KSLYPSIIRTFLIDPVGLVEGMAQPDPEHSTEGFLDAWFSREKHCLPEIVSQIWHGRDEA
HHHHHHHHHHHHCCCHHHHHCCCCCCCCCCCHHHHHHHHCCCCCHHHHHHHHHHCCCHHH
KRQGNKPLSQALKIIMNAFYGVLGTTACRFFDPRLASSITMRGHAIMRQTKALIEAQGYN
HHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCE
VIYGDTDSTFVWLRRAHSEADAAEIGHRLVRHVNEWWAQTLQQQNLTSALELEFETHFCR
EEECCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEHHHHHHHH
FLMPTIRGADTGSKKRYAGLIQEGDSQRMVFKGLETVRTDWTPLAQRFQQELYLRVFRNE
HHHHHHCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCC
PYQDYVRETIDKLMAGELDAQLVYRKRLRRPLHEYQRNIPPHVRAARLADEQNLKRGRPA
CHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCHHHHCCCCCC
QYQNRGAIKYVWTVNGPEPVDYQQSPLDYEHYLTRQLQPVAEGILPFVEDNFATLLTGQL
CCCCCCCEEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHCHH
GLF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2034216; 2261080; 1630901; 9278503; 2217198; 9079692 [H]