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The map label for this gene is rnt [H]

Identifier: 194737431

GI number: 194737431

Start: 1475617

End: 1476264

Strand: Reverse

Name: rnt [H]

Synonym: SeSA_A1531

Alternate gene names: 194737431

Gene position: 1476264-1475617 (Counterclockwise)

Preceding gene: 194737858

Following gene: 194736408

Centisome position: 31.35

GC content: 53.7

Gene sequence:

>648_bases
ATGTCCGATAACGCTCAACTTTCCGGTCTGTGCGACCGTTTTCGTGGTTTTTATCCTGTCGTCATCGACGTTGAAACTGC
AGGATTTAACGCTAAAACCGATGCGCTGCTCGAAATCGCCGCCATCACGCTGAAAATGGATGAACAAGGCTGGCTGATGC
CGGACATGACGCTGCATTTCCATGTGGAGCCATTCGCTGGAGCCAACTTACAGCCGGAAGCGCTCGCGTTTAACGGTATC
GATCCCTCTAACCCGCTACGCGGCGCGGTGAGTGAATATGAGGCGCTACACGCTATTTTCAAAATGGTGCGTAAAGGTAT
CAAAGACAGCGGTTGTAGCCGCGCCATTATGGTCGCGCATAACGCCACTTTTGATCACAGTTTTATGATGGCCGCCGCAG
AGCGCGCCTCGCTGAAACGTAACCCGTTTCATCCGTTCGTCACCTTTGATACCGCGGCATTGAGCGGACTGGCGCTGGGA
CAAACGGTGTTATCAAAAGCGTGCCTGGCGGCAGGTATGGAGTTTGACGGCGAAAAGGCCCATTCCGCTTTGTATGATAC
CGAGCGGACAGCGGTACTGTTTTGTGAAATCGTCAATCGCTGGAAGCGCCTGGGCGGCTGGCCGTTACCTTTGCCGACGG
ACAAATAA

Upstream 100 bases:

>100_bases
TCCCCCGCCGGGCGCAGGTCACTGCGCCCGTTGTTTTACTGCATCCCGGCGTAAAATTTGCCATAATACGCGCTGCATTT
TTTCGTATTAAGAGACTCAG

Downstream 100 bases:

>100_bases
CGTGGCTAACGACGCCGCTTTTGCTCACGATGGCAGTAAAAAAGCGACCTTTACAGGTCGCTTTCTCCTTTCACAGATTA
CTCTGCGTCCGGCTCCTGAG

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSDNAQLSGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEQGWLMPDMTLHFHVEPFAGANLQPEALAFNGI
DPSNPLRGAVSEYEALHAIFKMVRKGIKDSGCSRAIMVAHNATFDHSFMMAAAERASLKRNPFHPFVTFDTAALSGLALG
QTVLSKACLAAGMEFDGEKAHSALYDTERTAVLFCEIVNRWKRLGGWPLPLPTDK

Sequences:

>Translated_215_residues
MSDNAQLSGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEQGWLMPDMTLHFHVEPFAGANLQPEALAFNGI
DPSNPLRGAVSEYEALHAIFKMVRKGIKDSGCSRAIMVAHNATFDHSFMMAAAERASLKRNPFHPFVTFDTAALSGLALG
QTVLSKACLAAGMEFDGEKAHSALYDTERTAVLFCEIVNRWKRLGGWPLPLPTDK
>Mature_214_residues
SDNAQLSGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEQGWLMPDMTLHFHVEPFAGANLQPEALAFNGID
PSNPLRGAVSEYEALHAIFKMVRKGIKDSGCSRAIMVAHNATFDHSFMMAAAERASLKRNPFHPFVTFDTAALSGLALGQ
TVLSKACLAAGMEFDGEKAHSALYDTERTAVLFCEIVNRWKRLGGWPLPLPTDK

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=209, Percent_Identity=90.9090909090909, Blast_Score=400, Evalue=1e-113,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.-

Molecular weight: Translated: 23535; Mature: 23404

Theoretical pI: Translated: 6.15; Mature: 6.15

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
4.2 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDNAQLSGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEQGWLMPDMTLHF
CCCCCHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHEEEEEEEEECCCCCCCCCCEEEE
HVEPFAGANLQPEALAFNGIDPSNPLRGAVSEYEALHAIFKMVRKGIKDSGCSRAIMVAH
EECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
NATFDHSFMMAAAERASLKRNPFHPFVTFDTAALSGLALGQTVLSKACLAAGMEFDGEKA
CCCCCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
HSALYDTERTAVLFCEIVNRWKRLGGWPLPLPTDK
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
SDNAQLSGLCDRFRGFYPVVIDVETAGFNAKTDALLEIAAITLKMDEQGWLMPDMTLHF
CCCCHHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHEEEEEEEEECCCCCCCCCCEEEE
HVEPFAGANLQPEALAFNGIDPSNPLRGAVSEYEALHAIFKMVRKGIKDSGCSRAIMVAH
EECCCCCCCCCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEE
NATFDHSFMMAAAERASLKRNPFHPFVTFDTAALSGLALGQTVLSKACLAAGMEFDGEKA
CCCCCHHHHHHHHHHHHHCCCCCCCEEEECHHHHHHHHHHHHHHHHHHHHHCCCCCCHHH
HSALYDTERTAVLFCEIVNRWKRLGGWPLPLPTDK
HHHHHCCCCHHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA