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The map label for this gene is gidA [H]

Identifier: 194737365

GI number: 194737365

Start: 3957909

End: 3959798

Strand: Reverse

Name: gidA [H]

Synonym: SeSA_A4086

Alternate gene names: 194737365

Gene position: 3959798-3957909 (Counterclockwise)

Preceding gene: 194737178

Following gene: 194734008

Centisome position: 84.09

GC content: 55.61

Gene sequence:

>1890_bases
ATGTTTTATCAGGATCCTTTTGACGTCATTATTATTGGCGGGGGTCATGCAGGCACTGAGGCCGCGATGGCCGCGGCGCG
TATGGGTCAACAGACTCTGCTTTTGACACACAATATTGACACGTTGGGACAGATGAGCTGCAACCCGGCGATCGGCGGTA
TTGGAAAGGGACATCTGGTAAAAGAGGTGGATGCGCTCGGCGGTCTGATGGCGAAAGCGATCGATCAGGCAGGTATTCAG
TTTAGGATACTAAACGCCAGCAAAGGTCCGGCCGTTCGCGCTACCCGGGCTCAGGCGGATCGAGTCTTATACCGCCAGGC
GGTGCGTACCGCGCTGGAGAACCAGCCGAATCTGATGATCTTCCAGCAAGCGGTTGAAGATCTGATTGTCGAAAACGATC
GCGTTGTCGGCGCAGTGACCCAAATGGGGCTCAAATTCCGCGCGAAAGCGGTGGTGCTGACCGTCGGAACCTTCCTGGAC
GGCAAAATTCATATTGGTCTGGATAACTACAGCGGCGGTCGTGCTGGCGATCCGCCATCCATCCCGCTGTCTCGCCGTCT
GCGTGAGCTCCCGCTACGCGTCAGCCGCCTGAAAACCGGCACACCGCCGCGTATTGATGCGCGTACTATCGATTTCAGCG
TGTTGGCTCAACAGCATGGCGATAACCCGATGCCGGTTTTCTCGTTTATGGGGAATGCTTCTCAGCATCCGCAACAGGTG
CCGTGTTACATCACGCATACCAATGAGAAAACCCATGACGTGATTCGTAATAACCTCGATCGTAGCCCAATGTACGCTGG
CGTGATCGAAGGGATCGGCCCACGCTACTGTCCTTCGATCGAAGACAAGGTGATGCGTTTTGCCGATCGTAACCAGCATC
AGATCTTTCTTGAGCCGGAAGGACTGACCTCTAACGAGATTTACCCTAACGGTATCTCCACCAGCCTGCCGTTTGACGTG
CAGATGCAGATTGTCCGTTCCATGCAGGGTATGGAGAACGCGAAGATCGTTCGCCCTGGTTACGCGATTGAGTACGATTT
CTTCGACCCGCGCGATCTGAAACCGACTCTGGAAAGTAAATTTATTCACGGCCTGTTCTTTGCTGGTCAAATCAACGGTA
CTACCGGCTATGAAGAGGCTGCGGCGCAGGGCTTGCTCGCTGGTCTGAATGCCGCGCGTCTTTCCGCCGACAAAGAGGGC
TGGGCGCCTGCGCGCTCTCAGGCTTATCTCGGCGTGCTGGTGGACGATCTCTGTACGCTGGGCACCAAAGAGCCGTATCG
CATGTTTACCTCCCGCGCCGAATATCGCCTGATGCTGCGTGAGGACAACGCCGATCTGCGTCTGACCGAAATGGGCCGCG
AGCTGGGTCTGGTGGATGATGAACGCTGGGCGCGCTTCAACGAGAAGCTGGAAAATATCGAACGCGAACGTCAGCGGCTG
AAATCGACATGGGTAACGCCGTCTGCGGAATCCGCCGATGAAGTGAATACGCATTTGACCACGCCTCTCTCCCGTGAGGC
CAGCGGCGAAGATCTGTTACGTCGTCCGGAAATGACCTACGCGCAGCTCACTTCGCTTGCCGCATTCGCGCCTGCGCTTG
AAGATGAGCAAGCCGCTGAGCAGGTGGAAATCCAGGTCAAATACGAAGGGTATATCGCTCGCCAGCAGGATGAGATTGAA
AAACAACTACGTAACGAGAATACGTTGCTGCCTGCCACGCTGGATTATCGTCAGGTTTCCGGTCTGTCGAACGAAGTTAT
CGCCAAGCTGAACGATCATAAACCGGCGTCTATCGGCCAGGCATCGCGTATCTCCGGCGTTACGCCTGCGGCAATCTCCA
TTCTGCTGGTATGGCTGAAAAAACAAGGTATGCTGCGTCGTAGCGCTTGA

Upstream 100 bases:

>100_bases
TAGCCCGGATCCCGGGGCTTTCTCCATCGGCTAAAGTTGAGTAGAATCCACCGCCCGGGCTTCAATCCATTTTCACACCG
CGTTATGCGAGGCAATTACC

Downstream 100 bases:

>100_bases
TAACACTAATTTGGCGGGGGCGCTTCGCTTACCCGCCCTACAAATTTGTAGGCCCGATAAGCGAAAGCGCATCGGGCATT
TGATTATTAACAGGTAATGA

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 629; Mature: 629

Protein sequence:

>629_residues
MFYQDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVSRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
PCYITHTNEKTHDVIRNNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESKFIHGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSADKEG
WAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEMGRELGLVDDERWARFNEKLENIERERQRL
KSTWVTPSAESADEVNTHLTTPLSREASGEDLLRRPEMTYAQLTSLAAFAPALEDEQAAEQVEIQVKYEGYIARQQDEIE
KQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGMLRRSA

Sequences:

>Translated_629_residues
MFYQDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVSRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
PCYITHTNEKTHDVIRNNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESKFIHGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSADKEG
WAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEMGRELGLVDDERWARFNEKLENIERERQRL
KSTWVTPSAESADEVNTHLTTPLSREASGEDLLRRPEMTYAQLTSLAAFAPALEDEQAAEQVEIQVKYEGYIARQQDEIE
KQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGMLRRSA
>Mature_629_residues
MFYQDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLVKEVDALGGLMAKAIDQAGIQ
FRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMIFQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLD
GKIHIGLDNYSGGRAGDPPSIPLSRRLRELPLRVSRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
PCYITHTNEKTHDVIRNNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADRNQHQIFLEPEGLTSNEIYPNGISTSLPFDV
QMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESKFIHGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSADKEG
WAPARSQAYLGVLVDDLCTLGTKEPYRMFTSRAEYRLMLREDNADLRLTEMGRELGLVDDERWARFNEKLENIERERQRL
KSTWVTPSAESADEVNTHLTTPLSREASGEDLLRRPEMTYAQLTSLAAFAPALEDEQAAEQVEIQVKYEGYIARQQDEIE
KQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQASRISGVTPAAISILLVWLKKQGMLRRSA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=634, Percent_Identity=47.3186119873817, Blast_Score=525, Evalue=1e-149,
Organism=Homo sapiens, GI19882217, Length=659, Percent_Identity=45.5235204855842, Blast_Score=511, Evalue=1e-145,
Organism=Homo sapiens, GI183227703, Length=674, Percent_Identity=44.5103857566766, Blast_Score=505, Evalue=1e-143,
Organism=Escherichia coli, GI2367273, Length=629, Percent_Identity=97.456279809221, Blast_Score=1257, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=642, Percent_Identity=40.6542056074766, Blast_Score=478, Evalue=1e-135,
Organism=Saccharomyces cerevisiae, GI6321202, Length=619, Percent_Identity=45.2342487883683, Blast_Score=512, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24658174, Length=643, Percent_Identity=46.8118195956454, Blast_Score=526, Evalue=1e-149,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 69671; Mature: 69671

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYQDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCCCCCCCCHHH
KEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMI
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEH
FQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLDNYSGGRAGDPPS
HHHHHHHHHHCCCCEEHHHHHHCHHHCCEEEEEEEECEECCEEEEEECCCCCCCCCCCCC
IPLSRRLRELPLRVSRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
CCHHHHHHHCCHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
PCYITHTNEKTHDVIRNNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADRNQHQIFLEPE
CEEEEECCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCCHHHHH
FIHGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSADKEGWAPARSQAYLGVLVDDLCTL
HHHHEEEEEEECCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
GTKEPYRMFTSRAEYRLMLREDNADLRLTEMGRELGLVDDERWARFNEKLENIERERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
KSTWVTPSAESADEVNTHLTTPLSREASGEDLLRRPEMTYAQLTSLAAFAPALEDEQAAE
HHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCHHCCC
QVEIQVKYEGYIARQQDEIEKQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQ
EEEEEEEECCEEECCHHHHHHHHCCCCCEECEECCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGVTPAAISILLVWLKKQGMLRRSA
HHHCCCCCHHHHHHHHHHHHHCCCHHCCC
>Mature Secondary Structure
MFYQDPFDVIIIGGGHAGTEAAMAAARMGQQTLLLTHNIDTLGQMSCNPAIGGIGKGHLV
CCCCCCCCEEEECCCCCCHHHHHHHHHCCCEEEEEEECCCHHHCCCCCCCCCCCCCCHHH
KEVDALGGLMAKAIDQAGIQFRILNASKGPAVRATRAQADRVLYRQAVRTALENQPNLMI
HHHHHHHHHHHHHHHHCCCEEEEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCCCCEEH
FQQAVEDLIVENDRVVGAVTQMGLKFRAKAVVLTVGTFLDGKIHIGLDNYSGGRAGDPPS
HHHHHHHHHHCCCCEEHHHHHHCHHHCCEEEEEEEECEECCEEEEEECCCCCCCCCCCCC
IPLSRRLRELPLRVSRLKTGTPPRIDARTIDFSVLAQQHGDNPMPVFSFMGNASQHPQQV
CCHHHHHHHCCHHHHHHCCCCCCCCCCEEEHHHHHHHHHCCCCCCHHHHCCCCCCCCCCC
PCYITHTNEKTHDVIRNNLDRSPMYAGVIEGIGPRYCPSIEDKVMRFADRNQHQIFLEPE
CEEEEECCCHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCEEEECCC
GLTSNEIYPNGISTSLPFDVQMQIVRSMQGMENAKIVRPGYAIEYDFFDPRDLKPTLESK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCEEECCCEEEEECCCCCCCCCCHHHHH
FIHGLFFAGQINGTTGYEEAAAQGLLAGLNAARLSADKEGWAPARSQAYLGVLVDDLCTL
HHHHEEEEEEECCCCCHHHHHHHHHHHCCCHHHHCCCCCCCCCCCCCHHHHHHHHHHHHC
GTKEPYRMFTSRAEYRLMLREDNADLRLTEMGRELGLVDDERWARFNEKLENIERERQRL
CCCCHHHHHHCCCCEEEEEEECCCCEEHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHH
KSTWVTPSAESADEVNTHLTTPLSREASGEDLLRRPEMTYAQLTSLAAFAPALEDEQAAE
HHHCCCCCCCCHHHHHHHCCCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCCCCHHCCC
QVEIQVKYEGYIARQQDEIEKQLRNENTLLPATLDYRQVSGLSNEVIAKLNDHKPASIGQ
EEEEEEEECCEEECCHHHHHHHHCCCCCEECEECCHHHHCCCCHHHHHHCCCCCCCCCCC
ASRISGVTPAAISILLVWLKKQGMLRRSA
HHHCCCCCHHHHHHHHHHHHHCCCHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA